• Title/Summary/Keyword: Dk

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Isolation and Identification of Alkalophilic Microorganism Producing Xylanase (Xylanase를 생산하는 호알칼리성 균주의 분리 및 동정)

  • Choi, Ji-Hwi;Bai, Dong-Hoon
    • Food Engineering Progress
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    • v.14 no.3
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    • pp.263-270
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    • 2010
  • An alkalophilic microorganism named DK-2386, which produces xylanase, was isolated from soil of Taejo-mountain, Cheonan-si, Chungnam, Korea. The isolated strain was characterized as Gram-positive, with size of 0.4${\times}$2.5 ${\mu}$m, spore forming, anaerobic, catalase positive, possessed with hydrolysis abilities of casein, starch, sodium carboxy methyl cellulose, and xylan, reduction of nitrate to nitrite, resistant against lysozyme, urease positive, and motility positive. The color of culture broth was reddish yellow. The strain DK-2386 was identified as Bacillus agaradhaerens by whole cell fatty-acid composition analysis and 16S rDNA sequence analysis. However, it was not identical to Bacillus agaradhaerens 40952 obtained from the Korean Culture Center of Microorganism in its colour of culture broth. Therefore, we have named the newly isolated strain as Bacillus agaradhaerens DK-2386.

An Excel Program for Dk Calculation of Contact Lens (엑셀 프로그램을 이용한 콘택트렌즈의 Dk 결정)

  • Kim, Dae Soo
    • Journal of Korean Ophthalmic Optics Society
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    • v.16 no.1
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    • pp.107-116
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    • 2011
  • Purpose: The review article was written to establish an excel program that could calculate minimal Dk of contact lens without $O^2$ deficiency and actual $O^2$ concentration on cornea when contact lens were being fitted by changing lens-related factors. Methods: An excel program was formulated to calculate the thickness of post-lens lacrimal layer, Dk of contact lens and $O^2$ concentration on cornea. Results: With the excel program established, minimal $O^2$ concentration needed on cornea could be calculated when the thickness of post-lens lacrimal layer was changed by varying lens-related factors. A different route in the excel program was needed to choose based on the shape of lacrimal layer. The thickness of lacrimal layer was determined by the shape of meniscus made of tear between lens edge and cornea with flat fit. Thus, the $O^2$ concentration showing negative number in calculation decreased on peripheral cornea with flatter fitting and actual $O^2$ concentration would be zero on cornea. With tight fitting, the thickness of post-lens lacrimal layer is much thicker than lens itself thus negative number in calculation by the excel program is shown indicating zero oxygen on cornea. It can cause $O^2$ deficiency regardless of Dk of contact lens. Conclusions: The calculation of thickness of post-lens lacrimal layer and $O^2$ concentration on cornea by the established excel program is suggested to avoid $O^2$ deficiency when fitting state is varied by changing lens-related factors.

Isolation of a Bacillus licheniformis DK42 Producing Cellulase and Xylanase, and Properties of the Enzymes (Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성)

  • Kim, Min Jeong;Lim, Soo Jin;Kang, Dae-Kyung
    • Journal of Animal Science and Technology
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    • v.50 no.3
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    • pp.429-436
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    • 2008
  • A bacterium producing cold-active cellulase and xylanase was isolated from pig feces. The isolate, DK42 strain, was found to be the Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus licheniformis DK42 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The characterization of crude cellulase and xylanase from B. licheniformis DK42 was investigated. Cellulase exhibited an optimum temperature and pH at 45℃ and 6.0, whereas xylanase exhibited an optimum temperature and pH at 55℃ and 6.0. Especially cellulase maintained approx. 50% of its maximum activity even at 10℃, indicating that it is cold-active. Both cellulase and xylanase were stable after 2hr at 35℃, whereas they lost their activities after 30min at 65℃.

Comparative Study on the Productivity for Silage Corn (Zea mays L.) Variety Certified Import Adaptability in Pyeongchang Area (사일리지용 옥수수 수입적응성 인증 품종의 평창지역 생산성 비교 연구)

  • Kim, Jong Geun;Li, Yu Wei;Park, Hyung Soo;Kim, Jong Duk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.2
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    • pp.161-167
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    • 2017
  • This experiment was conducted to comparison study on the productivity for certified varieties of import adaptability of silage corn in Pyeongchang area. Total eight varieties (Gangpyeongok, 31N27, 32P75, 32W86, P3156, P3394, DK 689 및 DK 729) were evaluated. The experimental design was 8 treatment of randomized block with three replications. Corn varieties were cultivated in experimental field of Pyeongchang campus, SNU from 1 May to 2 September, 2015 and plot size was $15m^2$. Plant height of 32W86 and ear height of Gwanpyeongok was the highest (p<0.05). Tasseling and silking date was 27 July-3 August and silking occurred after 1-3 day of tasseling. Average day to silking was 92 days and that of 31N27 variety was short (p<0.05). The varieties of DeKalbo Company (DK 689 and DK 729) required more times to silking. Average GDD (growth degree day) of eight varieties was $1,023^{\circ}C$ and P3352 was the lowest GDD. In the trials of resistance evaluation, P3394 was strong in disease, P3156 was the highest in insect. All varieties did not show the lodging and variety of DK 729 showed the highest stay green score (p<0.05). Average dry matter content was 30.77%, it showed higher trend in DM. 32W86 was the highest DM content among the varieties, but there was no significant difference among varieties (p>0.05). The weight per ear was the highest in 32W86 and the lowest in Gwanpyeongok. The ration of ear to whole plant was higher in 32W86 and P3394, but it was not found the significant difference (p>0.05). Average yield of fresh and DM was 59,017 and 13,476 kg/ha, respectively. DK 689 showed higher DM and TDN yield than others, but there was not significant difference (p>0.05). According to results, the difference of productivity was not found among certified variety of import adaptability of silage corn. The varieties Gwanpyeongok, 32W86 and 32P75 would be recommendable in Pyeongchang area for stable cultivation.

Immune Responses of NIH Mice Infected with Avirulent and Virulent Strains of Plasmodium chabaudi adami Single and Mixed Infections

  • Namazi, M.J.;Phillips, R.S.
    • Parasites, Hosts and Diseases
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    • v.48 no.1
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    • pp.23-33
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    • 2010
  • An understanding of the nature of the immune response to asexual erythrocytic stages of malaria parasites will facilitate vaccine development by identifying which responses the vaccine should preferentially induce. The present study examined and compared the immune responses of NIH mice in either single or mixed infections with avirulent (DK) or virulent (DS) strains of Plasmodium chabaudi adami using the ELISA test for detecting and measurement of cytokines and antibody production. In both single and mixed infections, the study showed that both cell- and antibody-mediated responses were activated. In all experiments, an early relatively high level of IFN-$\gamma$ and IgG2a during the acute phase of the infection, and later elevation of IL-4 and IgG1, suggested that there was a sequential Th1/Th2 response. However, in the avirulent DK strain infection a stronger Th1 response was observed compared to the virulent DS strain-infection or in mixed infections. In the virulent DS infection, there was a stronger Th2 response compared to that in the DK and mixed infections. The faster proliferation rate of the virulent DS strain compared to the DK strain was also evident.

The Analysis of Space in the Multi-housing unit of the Winners in the German architectural competition since 1990 (최근 20년간 독일 공동주택설계경기 당선작의 단위세대 공간 구조 분석)

  • Cho, Ja-Yeon;Choi, Chan-Hwan
    • Korean Institute of Interior Design Journal
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    • v.21 no.2
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    • pp.163-173
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    • 2012
  • In this study, the spatial analysis of the multi-housing unit plans was investigated since 1990 for the winners at the architectural competition which was held nationwide in Germany. The investigation was carried out from January in 1990 to December in 2010. 201Unit plans were analyzed. Space syntax model is used as analyzing tool of the Unit plans. The results of the study indicate followings: 1) The interior spaces in 3LDK types and 4LDK types, ordered by high value of integration are; (1) corridor, (2) entrance hall, (3) living room, (4) dining room. 2) The value of integration for the kitchen is higher in L/DK type than in LD/K type, and that for the living room is higher in LD/K type than in L/DK type. The mean value of integration for L/DK type is higher than that for LD/K type. This means that traffic line efficiency of L/DK type is higher than that of LD/K type. 3) The value of integration for corridor and entrance hall is gradually lower in recent years. That is, the type of Unit plans are changing from corridor types to hall types.

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Model on the Oxygen Transmission of Contact Lenses according to the Fitting (콘택트렌즈의 산소투과에 대한 피팅의 영향)

  • Kim, Daesoo;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.8 no.2
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    • pp.1-11
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    • 2003
  • A mathematical model was proposed to analyze the oxygen diffusion reaching the cornea through the tear layers and contact lens based on Fick's law and the principle of continuity of the diffusion flux through the each layers. The model predicts how the parameters such as the thickness of tear layer on the cornea, both the Dk and thickness of contact lenses etc., affect the oxygen tension at cornea and oxygen flux entering the cornea. It is found that either too flat or too tightly fitted contact lenses can cause the oxygen deficiency inside the periphery of the cornea because of the reduction of oxygen flux resulted from too thickened tear layer.

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First Record of Two Euplotes Ciliates (Ciliophora: Spirotrichea: Euplotida) from Korea

  • Park, Mi-Hyun;Kim, Se-Joo;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.26 no.1
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    • pp.21-27
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    • 2010
  • Two marine euplotid ciliates, i.e. Euplotes cristatus Kahl, 1932 and E. minuta Yocom, 1930, were collected from the public waterfront of Incheon on the Yellow Sea and from the Songjeong Beach, Busan, in the Strait of Korea, respectively. These two species were verified as unrecorded species in Korea. These species were described based on live observation, protargol impregnation, and silver nitrate impregnation. In addition, the small subunit ribosomal DNA (SSU rDNA) sequences of the two species were compared with previously known sequences of the Euplotes species. Euplotes cristatus has an elongated oval form, size in vivo of $60-84{\times}38-68\;{\mu}m$, 35-50 adoral zone of membranelles (AZM), 10 frontoventral cirri (FVC), 5 transverse cirri (TC), 4-5 caudal cirri (CC), 8 dorsal kineties (DK), 10-16 dorsal cilia of middle DK, and silverline system of single-vannus type. Euplotes minuta has a small ovoid form ($44-53{\times}26-35\;{\mu}m$ in vivo), 31-41 AZM, 10 FVC, 5 TC, 4 CC, 9 DK, 10-12 dorsal cilia of middle DK, and silverline system of single-vannus type.

Guassian pdfs Clustering Using a Divergence Measure-based Neural Network (발산거리 기반의 신경망에 의한 가우시안 확률 밀도 함수의 군집화)

  • 박동철;권오현
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.5C
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    • pp.627-631
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    • 2004
  • An efficient algorithm for clustering of GPDFs(Gaussian Probability Density Functions) in a speech recognition model is proposed in this paper. The proposed algorithm is based on CNN with the divergence as its distance measure and is applied to a speech recognition. The algorithm is compared with conventional Dk-means(Divergence-based k-means) algorithm in CDHMM(Continuous Density Hidden Markov Model). The results show that it can reduce about 31.3% of GPDFs over Dk-means algorithm without suffering any recognition performance. When compared with the case that no clustering is employed and full GPDFs are used, the proposed algorithm can save about 61.8% of GPDFs while preserving the recognition performance.

THE STABILITY OF LINEAR MAPPINGS IN BANACH MODULES ASSOCIATED WITH A GENERALIZED JENSEN MAPPING

  • Lee, Sung Jin
    • Journal of the Chungcheong Mathematical Society
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    • v.24 no.2
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    • pp.287-301
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    • 2011
  • Let X and Y be vector spaces. It is shown that a mapping $f\;:\;X{\rightarrow}Y$ satisfies the functional equation $$(\ddag)\hspace{50}dk\;f\left(\frac{\sum_{j=1}^{dk}x_j}{dk}\right)=\displaystyle\sum_{j=1}^{dk}f(x_j)$$ if and only if the mapping $f$ : X ${\rightarrow}$ Y is Cauchy additive, and prove the Cauchy-Rassias stability of the functional equation ($\ddag$) in Banach modules over a unital $C^{\ast}$-algebra. Let $\mathcal{A}$ and $\mathcal{B}$ be unital $C^{\ast}$-algebras. As an application, we show that every almost homomorphism $h\;:\;\mathcal{A}{\rightarrow}\mathcal{B}$ of $\mathcal{A}$ into $\mathcal{B}$ is a homomorphism when $h((k-1)^nuy)=h((k-1)^nu)h(y)$ for all unitaries $u{\in}\mathcal{A}$, all $y{\in}\mathcal{A}$, and $n$ = 0,1,2,$\cdots$. Moreover, we prove the Cauchy-Rassias stability of homomorphisms in $C^{\ast}$-algebras.