• Fisheries and Aquatic Sciences
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• v.13 no.2
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• pp.133-139
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• 2010

The effects of suspended solids size on culture water quality were determined in a commercial recirculating aquaculture system (RAS) for Japanese eel, Anguilla japonica. The particulate phase of the culture water was serially divided into six size fractions using 300, 200, 100, 75, 45, and 26 ${\mu}m$ pore size stainless sieves. The total, dissolved, and particulate nitrogen and phosphorus, and suspended solids for each fraction were determined. The concentration ranges in the fractions were: total nitrogen, 164-148 mg $L^{-1}$; total phosphorus, 20.4-15.5 mg $L^{-1}$; and total suspended solids, 8.1-6.1 mg $L^{-1}$. The concentration of total nitrogen and total phosphorus decreased significantly (P<0.05) with a 26 ${\mu}m$ and 200 ${\mu}m$ filter pore size, respectively. Nutrients from dissolved organic substances were much higher than from particulates. Analysis of particle size fractionation and its effects on water quality is useful to estimate removal efficiencies of a commercial effluent screening device for solid management and development of solid removal systems.

• 한국해양학회지
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• v.7 no.1
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• pp.1-14
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• 1972

Seasonal changes in nutrients and suspended organic particulates were measured in the estuary of Nak-dong River in relation to the black laver bed. Monthly measurements of water temperature, pH, dissolved oxygen, nutrients ( $NH_4$-N, $NO_2$-N, $NO_3$\-N, $PO_4$-P and $SiO_2$-Si) and organic suspended particulates (organic carbon and nitrogen) were determined at five stations from February through December, 1970. PH varied 7.6-8.4 with an average of 8.0, and percent saturation of dissolved oxygen were 71-147% with an average of 100.8%. Studies gave evidence that Nak-dong River estuary is strongly enriched with nutrients. Concentrations of nutrients were: 0.13-12.54 ${\mu}g$-atoms/${\iota}$ (averaging 1.63 ${\mu}g$-atoms/${\iota}$) for $NH_4-N$, 0.12-2.09 ${\mu}g$-atoms/${\iota}$ (averaging 0.71 ${\mu}g$-atoms/${\iota}$) for $NO_2-N$, 3.46-56.79 ${\mu}g$-atoms/${\iota}$ (averaging 21.54 ${\mu}g$-atoms/${\iota}$) for $NO_3$-N, 4.04-57.90 ${\mu}g$-atoms/${\iota}$ (averaging 23.79 ${\mu}g$-atoms/${\iota}$) for total soluble nitrogen, 0.18-5.05 ${\mu}g$-atoms/${\iota}$ (averaging 0.96 ${\mu}g$-atoms/${\iota}$) for $PO_4$-P, and 18.33-133.29 ${\mu}g$-atoms/${\iota}$ (averaging 71.57 ${\mu}g$-atoms/${\iota}$) for $SiO_2$-Si, respectively. These nutrient levels were considerably higher compare with other productive laver beds of Wan do and Pyung-il Do in Korea or Ise Bay in Japan. Concentrations of suspended organic particulates varied 55-648 ${\mu}g/{\iota}$ (averaging 392 ${\mu}g/{\iota}$) with organic carbon, 30-155 ${\mu}g/{\iota}$ (averaging 92 ${\mu}g/{\iota}$) with organic nitrogen, and its carbon-nitrogen ratios were varied within 1.5-8.4 with an average of 4.6.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.273-278
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• 2002

Two-step fed-batch fermentations were carried out to overproduce Bacillus licheniformis maltogenic amylase (BLMA) in recombinant Escherichia coli. The first step was to increase the cell mass by controlling the feeding of a glucose solution, while the second step was designed to improve the amylase expression efficiency by supplementing organic nitrogen sources. The linear gradient feeding method was successfully adopted to maintain the glucose concentration below 0.2 g/l during the fed-batch mode, as effectively minimizing acetic acid formation. When the dissolved oxygen (DO) level became limiting, an accumulation of acetic acid and drastic decrease in specific BLMA productivity were observed. Glucose and organic nitrogen sources consisting of yeast extract and casein hydrolysate were simultaneously supplied in the pH-stat mode to further increase the specific BLMA expression efficiency. An organic nitrogen source consisting of 200 g/1 yeast extract and 100 g/1 casein hydrolysate was found to be the best among the various combinations tested. The feeding of an organic nitrogen source in the second-step fed-batch period was highly beneficial in enhancing the BLMA production. The optimized two-step fed-batch culture resulted in 78 g/l maximum dry cell mass and 443 U/ml maximum BLMA activity, corresponding to 1.5-fold increase in the dry cell mass and 3.7-fold enhancement in BLMA production, compared with the simple fed-batch fermentation.

• Journal of Environmental Science International
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• v.21 no.10
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• pp.1171-1179
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• 2012

Headwaters initiate material export to downstream environments. A nested headwater study examined the flux of dissolved constituents and water from a perennial stream and four ephemeral/intermittent streams in the Upper Gulf Coastal Plain of Mississippi. Water was collected during storm and baseflow conditions. Multiple linear regression was used to model constituent concentration and calculate flux. Event was the major source of water discharged from the ephemeral and intermittent streams however, baseflow was the major source for water discharged by the perennial stream during events. The perennial stream had an area weighted average yields of 10.1, 0.01, 1.03, 0.65 kg/ha/yr of DON (dissolved organic nitrogen), $NO_3^-$-N, $NH_4^+$-N and $PO_4^{-3}$, respectively while large variabilities existed between the ephemeral and intermittent streams. These findings highlight the importance of headwaters in protecting the low order drainage basins as a key to water quality within perennial streams.

• Microbiology and Biotechnology Letters
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• v.25 no.3
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• pp.311-316
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• 1997

A high xylitol producing yeast was isolated from the sludge of xylose manufacturing factory and then identified as Candida tropicalis DS-72 according to physiological properties. The strain was able to produce xylitol in a high concentration up to 72g/l from 100g/l xylose in 32 hours. Medium optimization for xylitol production by C. tropicalis DS-72 was performed. Effect of various nitrogen sources on xylitol production was investigated. Of nitrogenous compounds, yeast extract was the most suitable organic nitrogen nutrient for the enhancement of xylitol production. However, inorganic nitrogen resulted in a low cell concentration and did not produce xylitol. Effect of inorganic salts such as KH$_{2}$PO$_{4}$, and MgSO$_{4}$, 7H$_{2}$O on xylitol production was also studied. Optimal medium was selected as xylose 100g/l, yeast extract 10g/l, KH$_{2}$PO$_{4}$, 5 g/l and MgSO$_{4}$, 7H$_{2}$O 0.2 g/l. Xylitol of 88 g/l was produced from 100 g/l xylose in 30 hours using the optimal medium in a flask. In a fermentor, a fed-batch culture with 300g/l xylose was carried out. A final xylitol concentration of 240 g/l in the culture could be obtained in 43 hours of culture time by maintaining the high level of dissolved oxygen during growth phase and limiting the dissolved oxygen in the same culture during production phase. This result corresponded to a xylitol yield of 80% and a xylitol productivity of 5.58 g/1-h.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.635-644
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• 1994

A mathematical model was developed to predict the concentrations of various nutrients in supernatants during aerobic digestion which is suitable to be employed in small wastewater treatment plants with such advantages as low capital cost and stable process. Significant reactions were determined with observing the behavior of nitrgen and phosphorus, and the model equations were built up in the form of simultaneous differential equations considering Mass Balance. Laboratory batch experiments were carried out at $20^{\circ}C$ and pH $7.5{\pm}0.5$ on the aerobic digestion of waste activated sludge at different solid levels. Nonlinear regression analysis was performed to estimate various reaction rate constants. The developed model can predict the behavior of Biomass N, dissolved organic N, $NH_4{^-}$-N, $NO_x{^-}$-N, and Biomass P, dissolved organic P, $PO_4{^-}$-P in aerobic digestion process. In this study, the results of simulation showed that dissolved nutrients had more effects on supernatants than nutrients in biomass, and phosphorus was more effective on supernatants than nitrogen.

• Journal of Korean Society on Water Environment
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• v.37 no.6
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• pp.425-432
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• 2021

A tidal power plant (TPP) has been in operation since the end of 2011 to improve the water quality of Shihwa Coastal Reservoir (SCR). Tidal mixing rate increased 5.6 times after the TPP operation so that in this study, its effects on water quality was assessed through statistical analysis of long-term water quality monitoring data. It was found that the increased tidal mixing contributed to solving the hypoxia problem in the bottom water by preventing the summer stratification. The analysis also showed that the increased tidal mixing had different effects depending on the relative concentration difference for each water quality substances between the SCR and the outside of SCR. The average concentrations of some substances (chemical oxygen demand, total phosphorus, chlorophyll-a) with higher concentrations than the outside of SCR decreased due to the dilution effect, but the other substances (total nitrogen, dissolved inorganic nitrogen, dissolved inorganic phosphorus) with lower concentrations compared to the outside ones increased on the contrary. Factor analysis also showed a consistent result that the first factor accounting for the water quality was changed from the organic-related substances to the nutrient-related substances after the increased tidal mixing. These results imply that the focus of future water quality management needs shifting from the organic substances to the nutrients, particularly dissolved inorganic nutrients. Considering the effect of inflow seawater on the nutrients, the management area should be extended to cover not only SCR but also a certain area outside of SCR.

• Journal of Environmental Science International
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• v.17 no.6
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• pp.611-621
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• 2008

This study intends to provide the necessary basic data needed for predicting the water quality and examining changes in water quality on the basis of the hydrological changes: an outflow or the character of a flow by investigating the interaction of the parameters through the estimation of optimal parameters need for predicting the water quality of the dam basin and the sensitivity among those estimated parameters. Im-Ha Dam in the upstream area of the Nakdong River was selected for analysis, and the water quality survey data necessary for parameter estimation was based on the monthly water quality data (water temperature, BOD, T-N and T-P) between December 1, $2005{\sim}$November 31, 2006. K1C(the saturated growth rate of plant plankton), K1RC (endogenous respiratory quotient of plankton), KDC(deoxidized ratio), K71C(minealized ratio of dissolved organic phosphorus), K83C(mineralized ratio of dissolved organic nitrogen) have been considered as the factors of the water quality performed in this water quality simulation, that is, the most effective parameters on BOD, T-N and T-P. In the result of the analysis of the sensitivity, KDC(deoxidized ratio) was the most sensitively reacted parameter on BOD and it was K71C(mineralized ratio of dissolved organic phosphorus) and K83C(mineralized ratio of dissolved organic nitrogen) on T-N and T-P. It is considered that it will be possible to apply the most optimal parameter to an analysis of the water quality simulation at Im-Ha Ho basin in the goal year by examining the interaction of the parameters through the parameters sampling which are able to applicable to prediction of the water quality and the analysis of the its sensitivity, in the future, also the analysis on the basis of the hydrological conditions: an outflow or the character of a flow will be needed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.6B
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• pp.669-675
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• 2006

Intermittent aerobic digestion experiments using a sequencing batch reactor (SBR) were carried out in this study. Aeration ratio was found to be an important operation factor for the reduction of solids and nitrogen. As the sludge digested, organic nitrogen was released from the solids and oxidized to nitrate nitrogen. Biological denitrification was also significant and the denitrification rate was limited by aeration ratio. Under the condition of 0.25-0.75 of aeration ratio, acclimation of ammonia nitrogen was not observed and pH were preserved near neutral in the intermittent aerobic digestion. As the aeration ratio increased, solids reduction was increased whereas dissolved nitrogen removal was decreased. Based on the experiments, 17-2% of VSS reduction and over 80% of dissolved nitrogen removal were practicable by intermittent aerobic digestion using a SBR when the MSRT were designed 8-32 days and aeration ratio was operated about 0.25-0.75.

• Journal of Wetlands Research
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• v.11 no.3
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• pp.9-19
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• 2009

This research addressed nonpoint source (NPS) pollution characteristics in a reclaimed rice paddy field. The paddy has an area of around 2,998 ha and is divided as two sub-watersheds, whose areas are 1,181 ha and 1,817 ha, respectively. Monitoring of hydrologic runoff and NPS pollution was undertaken during three-month period from June to August, 2008. Totally, three sampling trips were made when rainfall depth were 66.0 mm, 23.5 mm, and 23.0 mm, respectively. Generally pollution load increased with the heavier cultivation activity in Korea. Exported pollutants from the rice paddy, including TSS, TN and TP, have same levels as forest discharge. Organic nitrogen is main pattern but it mainly exists in the forms of dissolved organic nitrogen (DON). For phosphorus, dissolved inorganic phosphorus (DIP) takes the main part although part of them is associated with fine particles. This is different compared with other watersheds, where particles-associated phosphorus is the main form.