• 수산해양기술연구
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• 제51권2호
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• pp.155-162
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• 2015

Abalone (Haliotis discus hannai) is a shellfish that feeds on kelp and, as a product, it can often achieve a high market value. However, the dissolved oxygen (DO) levels in coastal waters in Korea have been negatively impacted by pollution from many anthropogenic sources. Herein, a computational fluid dynamics (CFD) software package was used to analyze the distribution of the DO concentration within an abalone containment structure. A finite volume approach was used to solve the Reynolds-averaged Navier-Stokes equations combined with a $k-{\varepsilon}$ turbulence model to describe the flow. The distribution of DO was determined within the control volume domain, and the transport equations of the pollutants were interpreted using a CFD model. The CFD analysis revealed that more than 60% and 30% of the relative oxygen concentration in one and two containers, respectively, was maintained when the flow acts along the six sheets of polyethylene plates. Therefore, it is clear that the abalone plate shelters should be placed parallel to the flow.

• 수산해양기술연구
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• 제59권4호
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• pp.387-398
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• 2023

Conventional aquaculture faces declining productivity, shifting to recirculating aquaculture system (RAS), known for minimizing water usage and maintaining consistent water temperatures for year-round fish growth. Rainbow trout (Oncorhynchus mykiss), a globally important cold-water species and the third most farmed fish in inland waters of Korea, valued for its fecundity and rapid growth. Dissolved oxygen, an important environmental factor affecting fish production and economics, highlights the need for smart aquaculture practices. Since 2018, the rise of intelligent aquaculture platforms, incorporating information and communications technology (ICT), emphasizes the essential role of RAS implementation. This eight-week study aimed to determine the optimal dissolved oxygen concentration for rainbow trout in RAS, utilizing a device for continuous monitoring, control and record. Dissolved oxygen concentrations were set at 5-6 mg/L, 9-10 mg/L, 14-15 mg/L and 17-18 mg/L. The growth rate significantly decreased at 5-6 mg/L, with no significant differences in other experimental groups. In hematological analysis, growth hormone (GH) was significantly highest at 5-6 mg/L, followed by 9-10 mg/L while Insulin-like growth factor-1 (IGF-1) was significantly lowest at 5-6 mg/L. In conclusion, the optimal dissolved oxygen concentration for rainbow trout in RAS is approximately 9-10 mg/L. Higher concentrations do not contribute to further growth or profitability.

• 에너지공학
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• 제23권2호
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• pp.49-56
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• 2014

생물학적 하수처리공정에서 안정적인 방류수질 확보와 처리 비용을 적게 하는 것은 매우 중요하다. 하지만 유입수 유량 및 성분 농도의 변화와 미생물의 비선형적인 동특성, 기타 환경 요인에 의해서 최적의 운전 제어를 하기가 쉽지 않기 때문에, 기존의 하수처리장에서는 필요한 양 이상의 폭기 및 화학물질을 과량 주입하는 방법 등을 사용하였다. 본 연구에서는 포기조에서 미생물에 필요한 용존산 소농도를 유지함과 동시에 과폭기로 인한 전력 비용을 감소할 수 있는 최적 제어 방법을 제안하였다. 최적 제어를 위하여 실시간 미생물 호흡률 측정기(Oxygen uptake rate, OUR)을 이용하여 측정하였고, 실시간 호흡률 측정값을 바탕으로 현재 미생물에 필요한 최적 DO 농도를 제안하였으며, 이에 따라 공기송풍기가 자동제어될 수 있도록 구성하였다. 최적 제어 방법을 통하여 하수처리장 포기조의 공기 송풍량에 소요되는 전력비용을 절감할 수 있었다.

• Korean Chemical Engineering Research
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• 제52권5호
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• pp.581-586
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• 2014

하수처리장을 운영함에 있어 안정적인 방류수질 확보와 이에 따른 처리 비용을 최소화하는 것이 주요 목적이다. 하지만 유입수 유량 및 성분 농도의 변화와 미생물의 비선형적인 동특성, 기타 환경 요인에 의해서 최적의 운전 제어를 하기가 쉽지 않기 때문에, 기존의 하수처리장에서는 필요한 양 이상의 폭기 및 화학물질을 과량 주입하는 방법 등을 사용하였다. 본 연구에서는 포기조에서 미생물에 필요한 용존산소농도는 유지하면서 과폭기로 인한 전력 비용을 감소하는 최적 제어 방법을 제안하였다. 하수조성와 포기조 미생물의 호흡률은 실시간 미생물 호흡률 측정기(Oxygen uptake rate, OUR)를 이용하여 측정하였고, 실시간 호흡률 측정값을 바탕으로 현재 미생물에 필요한 최적 DO 농도를 제안하였다. 유입수 부하변동에 따라 변화하는 미생물 호흡에 필요한 산소량 만큼만 폭기하도록 구성함으로써, 방류수 수질기준을 만족함과 동시에 전력 비용을 최소화할 수 있는 방안을 제시하였다.

• 해양환경안전학회지
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• 제3권1호
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• pp.99-109
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• 1997

The in situ observations and the seawater analyses were conducted in July and August, 1996 for the purpose of describing the characteristics of organic pollution, dissolved oxygen distributions, and the evaluation of water quality in Mokpo harbour. The vertical density distribution of water column was found to be in stable structure with higher water temperature and lower salinity on surface layer at ebb tide in summer. In July, dissolved oxygen was shown to be oversaturated on surface and bottom layers, while in August, which was shown to be oversaturated on surface layer, and to be unsaturated on bottom layer as 68∼93% of saturation percentage. Dissolved oxygen of bottom layer in August was evaluated to be under the regular grades, based on Korean standards of seawater quality. In view of COD, the seawater quality of Mokpo harbour in summer was evaluated to be deteriorated due to organic wastes and graded to be the third class, and TSS of Mokpo harbour in summer was graded to be the second class, based on Korean standards of seawater quality. In particular, COD of surface layer in August was found to be under the regular grades. It is, therefore, necessary to take measures for the control of pollution loads and the proper management of seawater quality in Mokpo harbour. The distribution patterns of DO, COD, VSS and Chlorophyll-a on surface layer along the downstream center line from inner harbour to harbour entrance were similar to one another at ebb tide in August.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1989년도 한국자동제어학술회의논문집; Seoul, Korea; 27-28 Oct. 1989
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• pp.790-794
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• 1989

A novel control method involving an automatic tuning of digital PID controller parameters has been developed for better regulation of DO (dissolved oxygen) concentration in batch fermentation processes. Heuristic reasoning allows the PID controller to reach improved tuning decisions based upon the supervision of certain control performance indices in the same cognitive manner as in an expert control.

• 인간식물환경학회지
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• 제24권6호
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• pp.609-617
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• 2021

Background and objective: The nanobubbles remain stable in water, and it increased dissolved oxygen (DO) in the water that promotes the seed germination and the plant growth. We evaluated the seed germination and growth of sprouts (radish, wheat, and barley) and leafy vegetables (red mustard and pak choi) when irrigated with various DO of nanobubble water (NB). Methods: The oxygen NB was generated by surface friction and treated in 4 levels: NB 0% (control, DO 9.21 mg·L^-1), NB 20% (DO 15.40 mg·L^-1), NB 33% (DO 20.93 mg·L^-1), and NB 100% (DO 39.29 mg·L^-1). Results: The root length of radish and wheat increased more in NB 33% than the control plot. The fresh weight increased in NB 33% compared to the control plot in radish and wheat, and both fresh and dry weight increased more in NB 20%, NB 33%, and NB 100% than the control plot of barley. The leaf length and width of red mustard decreased more in NB 33% and NB 100% than the control plot and NB 20%, which indicated the leaf compactness. The fresh and dry weight of shoot and root increased more in NB 100% than the control plot in red mustard. In pak choi, the shoot fresh weight increased more in NB 100% than the control plot. In leafy vegetables, the germination rate of red mustard in NB 100% was higher than the control plot, however, it was not significantly different between oxygen NB plots in sprout vegetables. Conclusion: The results showed that the root growth and biomass increased after applying NB 33% in sprout vegetables. The leaf growth properties as the number of leaves and leaf size were not significantly different or decreased in NB treatments compared to control plots, but NB 100% (DO 39.29 mg·L^-1) effectively increased the root growth and plant biomass in leafy vegetables.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1690-1698
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• 2006

In order to investigate the effect of dissolved oxygen (DO) level on AVM $B_{1a}$ production by a high yielding mutant of Streptomyces avermitilis, five sets of bioreactor cultures were performed under variously controlled DO levels. Using an online computer control system, the agitation speed and aeration rate were automatically controlled in an adaptive manner, responding timely to the oxygen requirement of the producer microorganism. In the two cultures of DO limitation, the onset of AVM $B_{1a}$ biosynthesis was observed to casually coincide with the fermentation time when oxygen-limited conditions were overcome by the producing microorganism. In contrast, this phenomenon did not occur in the parallel fermentations with DO levels controlled at around 30% and 40% throughout the entire fermentation period, showing an almost growth-associated mode of AVM $B_{1a}$ production: AVM $B_{1a}$ biosynthesis under the environments of high DO levels started much earlier than the corresponding oxygen-limited cultures, leading to a significant enhancement of AVM $B_{1a}$ production during the exponential stage. Consequently, approximately 6-fold and 9-fold increases in the final AVM $B_{1a}$ production were obtained in 30% and 40% DO-controlled fermentations, respectively, especially when compared with the culture of severe DO limitation (the culture with 0% DO level during the exponential phase). The production yield ($Y_{p/x}$), volumetric production rate (Qp), and specific production rate (${\bar{q}}_p$) of the 40% DO-controlled culture were observed to be 14%, 15%, and 15% higher, respectively, than those of the parallel cultures that were performed under an excessive agitation speed (350 rpm) and aeration rate (1 vvm) to maintain sufficiently high DO levels throughout the entire fermentation period. These results suggest that high shear damage of the high-yielding strain due to an excessive agitation speed is the primary reason for the reduction of the AVM $B_{1a}$ biosynthetic capability of the producer. As for the cell growth, exponential growth patterns during the initial 3 days were observed in the fermentations of sufficient DO levels, whereas almost linear patterns of cell growth were observed in the other two cultures of DO limitation during the identical period, resulting in apparently lower amounts of DCW. These results led us to conclude that maintenance of optimum DO levels, but not too high to cause potential shear damage on the producer, was crucial not only for the cell growth, but also for the enhanced production of AVM $B_{1a}$ by the filamentous mycelial cells of Streptomyces avermitilis.

• Environmental Engineering Research
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• 제24권4호
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• pp.582-590
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• 2019

Currently, the waterfront facility was constructed in New Songdo City, South Korea. It has the various water leisure areas and especially an artificial urban canal with filtered seawater by re-circulating flow system. However, due to excessive amount of nutrients from seawater combined with complicated geometry, it is highly vulnerable to deterioration of water quality. In this study, flow characteristics and pollutant transport were analyzed with comprehensive numerical models, MIKE 3 FM and ECO-lab. Based on these numerical results, notable sampling points were selected for field measurements and comparison between modeling and measured results were conducted. In addition, the integrated water quality evaluation index, Water Quality Index was applied to analyze various water quality issues. We also set up scenarios to control the two kinds of water quality factors, dissolved oxygen (DO), and total phosphorus (TP). As a result, the effect of 20% reduction of TP was less than 10% and it was almost ineffective for a year but it was reduced by up to 40% in case of scenario which DO is increased by 20%. Therefore, it was recommended to control the DO concentration, usually by applying re-aeration facility, rather than TP in artificial urban canal with seawater.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.333-335
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• 2001

A simple control strategy of DO-stat was introduced to the recombinant rGuamerin production process in Pichia pastoris. This induction strategy consisted of two interrelated control loops ‘by which oxygen ratio of inlet gas and methanol feeding rate was controlled. Using this control strategy, over-feeding or under-feeding of methanol could be avoided in concomitance with the efficient control of dissolved oxygen level. As a result, the cell concentration reached 130 g/L and rGuamerin expression level was 450 iu/L, which was more than 40% increased result comparing with the fed-batch process using manual control of methanol feeding rate.