• 대한한의진단학회지
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• 제18권1호
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• pp.25-31
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• 2014

Objective This study aims to understand the actual utilization of the oriental rehabilitation medicine center of the initial interdisciplinary medical care at the National Rehabilitation Center and to arrange proper development plans for more effective interdisciplinary medical services for future activation of interdisciplinary medical care. Method: The interdisciplinary patient status, composition of patient care, and major disease-related status were studied and analyzed for oriental rehabilitation medication relating to interdisciplinary medical care at the National Rehabilitation Center. Furthermore, the interdisciplinary status and its operating conditions were evaluated to devise a development plan for the National Rehabilitation Center. Results: As a result of the analysis of oriental rehabilitation medical care diagnosing status utilization at the National Rehabilitation Center regarding interdisciplinary medical care, the ratio of the number of those receiving interdisciplinary oriental rehabilitation medical care was less than half of the total receiving interdisciplinary care. As the major disease status from the patients of interdisciplinary care, stroke patients covered the large majority, and musculoskeletal pain patients covered the majority of outpatients. The establishment of additional oriental medication departments and assigning of interdisciplinary coordinators were presented as the goals of a development plan for the manpower and structural side of interdisciplinary medical care at the National Rehabilitation Center. On the institutional and systematic side, improvement in the interdisciplinary hierarchy and interdisciplinary model development based on the number of patients per disease was proposed. In addition, operation on ward bedding and construction of interdisciplinary EMR medical care is necessary on the medical and administration service side. Conclusion: To understand the actual utilization and to arrange development plans aimed at constructing a safe and effective interdisciplinary hierarchy for interdisciplinary medical care at the National Rehabilitation Center, patient-centered care needs to be implemented.

• 동의생리병리학회지
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• 제22권1호
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• pp.194-198
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• 2008

The treatment before growing into disease is the highest level of the spirit of oriental preventive public medicine. Specially the treatmentt is very important in the life style disease of the aged society, and it is closely connected the human body-form diagnosis reflecting the character of individual physiology and pathology. The human body-form diagnosis explain systematically the color of face, the form and movement of the body, the smell and the taste, the mentality, many or few of $Yin{\cdot}Yang$, the state of pulse, the Meridian, the state of pathology and the direction of the treatment, and therefore the human body-form diagnostis method has also the practical ity on classifying the type. As the spirit of oriental preventive public medicine is based on the Meridian control method and food cooking control method, it has an important effect on the general management of the disease, the making on the beautiful face and the healthy body, the prolongation of life, the management of the individual-ordered health.

• 동의생리병리학회지
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• 제22권6호
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• pp.1509-1517
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• 2008

The flowers and buds of Lonicera Flower (LF), are used in Korean herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in immune reaction. These pharmaceutical effects of LF looks like to be related to its antioxidant capacity and phytochemicals containing in LF. In this study, the antioxidant activity of extract from LF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The LF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the LF extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• 대한수의학회지
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• 제49권2호
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• pp.121-125
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• 2009

Paratuberculosis caused by Mycobacterium avium subspecies paratuberculosis (Mpt) is a chronic infectious enteric disease with deleterious impact on the performance in ruminants. In Korea, ELISA has been introduced to detect antibodies to Mpt in individual cattle. However, comparison study with ELISA has not been studied until now. In total, a panel of 899 serum samples obtained from dairy cattle was analyzed with two commercial ELISAs for Mpt to assess the performance. Two ELISAs employed in this study were both licensed worldwide. Two ELISAs applied onto same serum samples showed the moderate agreement (kappa value = 0.60). There was non-significant McNemar test (p = 0.0614) between two ELISA results indicating that each proportion detected by two kits did not differ. In addition, the percent agreement between two ELISA results was turned out to be 96.8% which interpreted excellent reproducibility. It was shown from this study that two ELISAs revealed moderate kappa agreement performance. The implication raised is that when ELISAs as diagnostics are used to detect Mpt in individual cattle, positive reaction by either ELISA should be interpreted as serologically Mpt positive due to presumed low sensitivity of ELISAs and their test agreement being less than 100%.

• Journal of Microbiology and Biotechnology
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• 제29권9호
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• pp.1488-1493
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• 2019

The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.

• 한방비만학회지
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• 제24권1호
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• pp.1-12
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• 2024

Objectives: Ephedrae herba (EH) and Coicis semen (CS) has been frequently prescribed for the treatment of obesity. However, effects of combinational extracts of these two herbs on non-alcoholic fatty liver disease are unknown. The aim of the present study was to investigate the effects of EH and CS on lipid accumulation and glucose absorption in free fatty acids (FFAs) or palmitic acid (PA)-treated HepG2 cells. Methods: Five samples of EH and CS were extracted by combination ratios (S1=0:100, S2=25:75, S3=50:50, S4=75:25, S5=100:0). Oil Red O staining was used to measure lipid accumulation in FFAs-induced steatosis cells. Intracellular triglycerides and total cholesterol levels were measured in FFAs-induced steatotic HepG2 cells. In PA-treated cells, intracellular 2-NBDG was detected using a fluorescence microplate reader and flow cytometry. Phosphorylation of key metabolism-related factors of AMP-activated protein kinase and acetyl-CoA carboxylase, expression of key lipid synthesis-related factors carnitine palmitoyltransferase 1 alpha (CPT1α), sterol regulatory element-binding protein 1 (SREBP1), peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT enhancer binding protein alpha (C/EBPα) were confirmed by western blot. Results: Treatment of EH-CS combination in the FFAs-induced steatotic HepG2 cells significantly reduced lipid accumulation. As the relative ratio of Ephedrae herba increased, the lipid-lowering effects of the combination were increased. However, S1 and S5 of Ephedrae herba and Coicis semen did not significantly reduce triglycerides and total cholesterol induced by FFAs. However, the combination of Ephedrae herba and Coicis semen restored glucose absorption in PA-induced HepG2 cells. Major makers of SREBP1, PPARγ, C/EBPα, and CPT1α expression tended to decrease with EH ratio. Conclusions: The EH-CS combination has advantages over sole EH and CS extracts in improving lipid and glucose metabolism in liver steatosis models.

• 한국임상수의학회지
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• 제18권3호
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• pp.304-310
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• 2001

The joint disease including osteoarthris (OA) and rheumatoid arthritis (RA) are common in the horse. Many studies have been performed to develop biochemical markers reflecting the abnormalities of cartilage and synovial membrane. However, no specific, sensitive and clinically well established assay systems have been yet available to characterize the severity of joint diseases. Indeed, radiography is still doctor's best choice of assessing joint damage in OA/RA. This review focuses on biochemical molecules such as proteoglycan, collagen, matrix metalloproteinases (MMPs), lectin and cytokine to assess their potential value for not only predicting stage of joint disease but also monitoring treatment efficacy.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2023년도 추계학술발표대회
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• pp.557-560
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• 2023

In this paper, we introduce a pre-training method leveraging the capabilities of the Vision Transformer (ViT) for disease diagnosis in conventional Fundus images. Recognizing the need for effective representation learning in medical images, our method combines the Vision Transformer with a Masked Autoencoder to generate meaningful and pertinent image augmentations. During pre-training, the Masked Autoencoder produces an altered version of the original image, which serves as a positive pair. The Vision Transformer then employs contrastive learning techniques with this image pair to refine its weight parameters. Our experiments demonstrate that this dual-model approach harnesses the strengths of both the ViT and the Masked Autoencoder, resulting in robust and clinically relevant feature embeddings. Preliminary results suggest significant improvements in diagnostic accuracy, underscoring the potential of our methodology in enhancing automated disease diagnosis in fundus imaging.

• Clinical and Experimental Pediatrics
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• 제55권3호
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• pp.77-82
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• 2012

Human astrovirus (HAstV) is a major cause of acute diarrhea among children, resulting in outbreaks of diarrhea and occasionally hospitalization. Improved surveillance and application of sensitive molecular diagnostics have further defined the impact of HAstV infections in children. These studies have shown that HAstV infections are clinically milder (diarrhea, vomiting, fever) than infections with other enteric agents. Among the 8 serotypes of HAstV identified, serotype 1 is the predominant strain worldwide. In addition to serotype 1, the detection rate of HAstV types 2 to 8 has increased by using newly developed assays. HAstV is less common compared with other major gastroenteritis viruses, including norovirus and rotavirus; however, it is a potentially important viral etiological agent with a significant role in acute gastroenteritis. A better understanding of the molecular epidemiology and characteristics of HAstV strains may be valuable to develop specific prevention strategies.

• 동국한의학연구소논문집
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• 제7권2호
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• pp.47-67
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• 1999

상한론(傷寒論)은 복증(腹證)에 관한 내용이 전체의 1/4에 달할 정도로 重히 다루고 있으며 방증변증(方證辨證)이 중심이 되어 복증(腹證)을 통한 변증이 매우 발달되어 있어서 진단학(診斷學)의 발전에 크게 기여하였다. 이후 진단학診斷學)의 발전(發展)은 진맥(診脈), 진설(診舌)을 위주로 했으며, 복진(腹診)의 운용(運用)에 대해서는 역사적으로 사회적인 특수한 배경으로 인하여 계속 발전되지 못하였다. 최근 한의학적(韓醫學的) 진단방법(診斷方法)과 치료방법(治療方法)이 매우 강조되어 활발한 연구가 계속 진행되면서 복증변증(腹證辨證)에 관한 관심이 매우 집중되고 있기에, 복부진단(腹部診斷)에 관한 연구(硏究)의 한 방법(方法)으로써 상한론(傷寒論) 조문(條文)에 실려있는 상견복증(常見腹證)에 관련된 내용(內容)을 정리한 결과(結果) 약간의 지견(知見)을 얻었다.