Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity

  • Jiang, Huofeng (School of Life Sciences, University of Science and Technology of China) ;
  • Gao, Yamin (State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences) ;
  • Zeng, Sheng (State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences) ;
  • Wang, Shuai (State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences) ;
  • Cao, Zhizhong (State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital) ;
  • Tan, Yaoju (State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital) ;
  • Yin, Huancai (CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences) ;
  • Liu, Jianxiong (State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital) ;
  • Zhang, Tianyu (State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences)
  • Received : 2019.05.07
  • Accepted : 2019.08.01
  • Published : 2019.09.28
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Abstract

The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.

Keywords

References

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