• 제목/요약/키워드: Discrimination of origins

검색결과 36건 처리시간 0.023초

RAPD마커를 이용한 가시오갈피의 기원판별 및 유전적 다양성 (Discrimination and Genetic Diversity of Acanthopanax senticosus Using RAPD Markers)

  • 허만규;최영현;최병태;김경철
    • 동의생리병리학회지
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    • 제19권4호
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    • pp.1046-1049
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    • 2005
  • Acanthopanx senticosus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important woody plants in Korea. To identify the variation of the RAPD patterns between domestic and foreign A. senticosus species, 22 random primers were applied to Korean A. senticosus and A. senticosus for. inermis, Chinese and Russian A. senticosus. Six primers of them could be used to discriminate the origins and 58 polymorphisms among 92 scored DNA fragments. Six bands are specific for Korean A. senticosus and A. senticosus for. inermis. Especially, three primers, OPD04, OPD11 and OPE10, were useful to differentiate between domestic and foreign Acanthopanax species. RAPD analysis was a useful method to discriminate among A. senticosus populations or accessions and Korean accessions are distinct genetically.

당귀의 해부형태학적 특징에 따른 기원판별 (Discrimination of Angelicae Radix Based on Anatomical Characters)

  • 성정숙;방경환;박충헌;박춘근;유홍섭;박희운;성낙술
    • 한국약용작물학회지
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    • 제12권1호
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    • pp.67-72
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    • 2004
  • 당귀류 약재의 기원식물에 대해 해부형태적 특징을 관찰하고, 이를 통하여 당귀 기원 및 국내유통 당귀류 약재의 판별에 이용코자 실험한 결과는 다음과 같다. 기원식물 뿌리의 해부형태특징은 정상 2기생장, 주피발달, 유세포내 전분함유 등이 공통점이었고, 후각세포층은 참당귀와 왜당귀에서 관찰되었으며 피층의 유실분포는 참당귀와 중국당귀에서 관찰되었다. 또한 참당귀에서만 속성후벽세포가 다량 관찰되었다. 잎의 표피세포는 중국당귀가 가장 컸으며 세포표면의 형태는 참당귀와 중국당귀는 거친 주름이져 있었고, 왜당귀는 완만한 굴곡이 관찰되었다. 3종의 기공은 모두 불규칙형 부세포를 가지고 있었고 참당귀가 $mm^2$당 600개 이상으로 가장 많은 기공이 분포하고 있었다. 모용은 참당귀에서만 원뿔형으로 관찰되었다. 엽병은 3종식물에서 모두 유조직이 잘 발달된 형태였고 환상으로 병립유관속이 발달하고 있었다. 국내 유통 당귀류 약재의 판별에 있어서 해부형태적 특징을 정리하여 간단한 동정 KEY를 적용하여 만든 검색표가 유용하게 활용될 수 있었다. 검색표를 이용하여 전국 7개 지점에서 수집한 약재를 판별한 결과, 당귀 (국산) 또는 토당귀로 유통되는 약재는 참당귀 (A. gigas)로 동정되었고, 일당귀로 유통되는 약재는 왜당귀(A. acutiloba)로 동정되었다. 중국당귀는 현재 시중 유통되지 않고 있으며 본연구에서 사용된 약재 중국당귀는 중국 현지에서 직접 구입해 온 것으로 중국당귀 (A. sinensis)의 뿌리와 동일한 횡단면 형태를 관찰할 수 있었다. 그러므로, 현재 국내 유통되는 당귀류 약재는 (토)당귀의 경우는 KP에서 규정하는 것과 동일기원이었으며, 일당귀는 일본약국방(JP,2001)에서 규정하는 것과 동일한 기원으로 당귀류 유통약재의 기원상에 오류는 없었다.

X-선 형광 분석방법을 이용한 숙지황의 무기원소 함량분석과 지리적 특성 규명 (Discrimination of Geographic Origin by Trace Elements Contents in Rehmannia Radix Preparat using X-ray Fluorescence Analysis)

  • 배혜리;이시경;황인재;강정미;이진호;김정한
    • Journal of Applied Biological Chemistry
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    • 제58권4호
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    • pp.345-348
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    • 2015
  • 한국산 및 중국산 숙지황(Dried Rehmannia Radix Preparat)의 지리적 원산지에 따른 특성을 알아보기 위하여 X-선 형광분석기(X-ray fluorescence spectrometry)를 이용하여 미량 원소 함량을 측정하였다. 143종의 시료에서 검출된 K, P, S, Cl, Si, Al, Fe, Sn 등 35종의 원소를 정준판별분석한 결과 평균 판별 정확도는 92.3%로 나타났으며, 유의수준(p=)은 0.0001 미만으로 나타났다. 통계분석에 사용하는 원소수를 35종에서 8종, 3종으로 줄였을 때 판별정확도는 각각 88.1, 84.6%로 감소하는 것으로 나타났다. 중국산내에서의 판별정확도는 94.6-96.0%였으나 한국산내에서의 판별정확도는 72.5-89.9%로 나타났다.

근적외선분광법을 이용한 택사의 산지 판별법 연구 (Discrimination of Alismatis Rhizoma According to Geographical Origins using Near Infrared Spectroscopy)

  • 이동영;김승현;김효진;성상현
    • 생약학회지
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    • 제44권4호
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    • pp.344-349
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    • 2013
  • Near infrared spectroscopy (NIRS) combined with multivariate analysis was used to discriminate the geographical origin of Alisma orientale from Korea (n=94) and China (n=72). Two-thirds of samples were selected randomly for the training set, and one-third of samples for the test set. Second derivative was used for the pretreatment of NIR spectra. Partial least square discriminant analysis (PLS-DA) models correctly discriminated 100% of the Korean and Chinese A. orientale samples. These results demonstrate the potential use of NIR spectroscopy combined with multivariate analysis as a rapid and accurate method to discriminate A. orientale according to their geographical origin.

Metabolomics Approach for Classification of Medicinal Plants

  • Lee, Dong-Ho
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2010년도 정기총회 및 춘계학술발표회
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    • pp.5-5
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    • 2010
  • Selection of specific medicinal sources as well as bioactive compounds is important for the preparation of medicine and related products with good quality. It is necessary to pay close attention for choosing correct medicinal sources, particularly in case of medicinal plants, because of their diversity, which can affect the quality and efficacy of medicine. Discrimination of plants based on morphological or genetic characteristics has been used as a conventional classification method of pharmaceutical sources so far; however, more need demands more general methods for accurate quality assessment of medicinal plants. In this study, ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) technique applied to this metabolic profiling is a powerful tool due to its higher sensitivity, resolution, and speed compared to conventional HPLC technique. The metabolite profiling of several medicinal plants including Panax ginseng was carried out using UPLC/Q-TOF MS and total metabolites were then subsequently applied to various statistical tools to compare the patterns. The developed metabolomics tool with UPLC/Q-TOF MS successfully identified and classified the samples tested according to their origins.

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한국산 및 중국산 참깨로부터 착유한 참기름의 주요성분 비교 (Comparison of Major Components of Sesame Oil Extracted from Korean and Chinese Sesames)

  • 서정희;김제란;이기동;권중호
    • 한국식품위생안전성학회지
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    • 제11권3호
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    • pp.215-220
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    • 1996
  • Korean and Chinese sesames were subjected to microscopic observation and instrumental determination of fatty acid composition and sesamolin/sesamin ratio to obtain basic data for discriminating each other. The overall appearance of both samples was differently observed by stereo microscope (X8). Fatty acid composition of sesame oils, extracted from both samples with different roasting degrees, showed a similar pattern although Chinese samples cointained about 6% higher content of stearic acid and 47% lower content of linolenic acid then Korean ones. The sesamolin/sesamin ratio was remarkably lower(.039) in Chinese samples than Korean (0.67∼0.72). showing a variation depending on producing districts. Roasting degrees of raw sesames little influenced their composition of fatty acid and sesamolin/sesamin ratio. Based on the above results, it is considered that the comparison between domestic and Chinese sesames in view of their stearic and linolenic acid contents and sesamolin/sesamin ratio might be one of the potential criteria in discriminating their production origins.

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Classification based on Near-IR spectra with application to Cnidium Rhizome

  • Cho, Chang-H.;Ze, Keum-R.;Sung, Rak-S.;Lee, Jog-P.;Park, Ju-Y.;Park, Sang-Y.;Jung, Young-J.;Cho, So-Y.;Jin, Zhexiong
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.405.2-405.2
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    • 2002
  • A near infrared (NIR) method was developed to analyze specious diversity for morphologically similar umbelliferous herbal medicine. Cnidium officinale Makino. This herbal medicine has been widely used as 'chungung' without any discrimination of its quality and original plants. though it has the ambiguous origins of plants between various countries especially Korea. China and Japan. It is named by Cnidium officinale Makino in Korea and Japan. in comparison with Ligusticum chuanxiong Hort. in China. (omitted)

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한약재 품질 관리에 관한 연구 (Studies on the Administration of the Quality of Herbal Medicine)

  • 최선미;정희진;윤유식;이미영;최환수;성현제
    • 대한한의학회지
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    • 제21권3호
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    • pp.99-112
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    • 2000
  • Objective: This study was conducted to suggest a proper administration system of the quality of herbal medicine. Methods: Related literature was carefully inspected and discussions among specialists in the field of herbal medicine's growth, production, circulation and administration were conducted. Results: 1. The administration of herbal medicine's quality should be done in each stage of its circulation; growing stage, harvesting stage, importing stage, production stage, quality examination stage, wrapping stage, circulating stage, and administration stage. 2. A safety standard should be established for items, parts and origins of each medicinal herb. A standard for the identification of forged products and objective standards for quality discrimination should also be established. 3. A national system for the training of herbal medicine quality administration personnel and herbal medicine discrimination personnel should be established. A data base of standardized product specimens should be established and a pharmacopoeia of herbal medicine should be published as a standard of quality administration in herbal medicine. 4. Research into the following preliminary areas should be conducted to investigate quality adminstration of herbal medicine: research related to herbal medicine's raw materials and herbal medicinal drugs; quality estimation of raw materials and drugs; quality administration in oriental medical hospitals; herbal medicine administration law; herbal medicine administration organization; herbal medicinal pharmacology; and policy-related research for the quality improvement of domestically grown medicinal herbs. Conclusion: For the proper administration of herbal medicine's quality, governmental support and intensive research among specialists should be done for the supply of good quality medicinal herbs and the improvement of the efficacy of herbal medicine.

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브라질의 인종과 정치: 발생, 전개, 특징 (Race and Politics in Brazil: Occurrence, Development, Characteristics)

  • 김달관
    • 이베로아메리카
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    • 제21권1호
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    • pp.1-55
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    • 2019
  • 브라질에서는 인종에 대한 명확한 개념의 규정이 존재하지 않으면서도 인종차별주의가 존재한다. 즉 명확한 인종의 개념이 없이 인종주의는 존재하기 때문에, 브라질에서 인종문제는 복잡하다고 하겠다. 인종주의는 단순한 사회적 문제가 아니다. 그것은 무엇보다도 경제적 분리, 배제, 주변화 등으로 나타나는 정치적 문제이다. 유럽 식민주의 확대 과정에서부터 인종주의는 브라질의 사회적, 물질적, 심리적, 정치적 지배의 가장 뿌리 깊고 효율적인 통치방식이었다. 이런 관점에서 본 연구는 인종의 정치로서 브라질의 인종주의에 대해 연구하려 한다. 결론적으로 브라질에는 인종에 따른 인종 차별과 인종불평등이 존재한다는 것이다. 이에 따라, 브라질에는 인종에 따른 인종차별과 인종불평등이 존재한다는 것을 연구하기 위해, 제2장은 브라질에서 인종정치의 기원을 살펴본다. 제3장에서는 브라질 사회와 인종에 대해 살펴보고자 한다. 제4장은 브라질의 흑인운동으로서 인종주의에 대한 흑인의 저항을 살펴본다. 제5장의 전반부는 '브라질의 인종불평등'을 살펴보고, 후반부는 '브라질의 인종차별'에 대해 살펴보려 한다. 제6장은 결론부분으로서 브라질의 인종정치의 도전과 한계는 무엇인지 논의하려 한다.

Chemotype Discrimination and Rapid Identification of Angelica Roots by DART-TOF-MS

  • Kim, Hye-Jin;Piao, Xiang-Lan;Jang, Young-Pyo
    • Natural Product Sciences
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    • 제17권3호
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    • pp.202-205
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    • 2011
  • The Angelica root has been used as a medicinal herb in many Asian countries including Korea, China, and Japan. Angelica gigas, A. sinensis, and A. acutiloba have been considered as Angelicae radix in Korean, Chinese, and Japanese Pharmacopoeia, respectively. Since the origins of Angelicae radix differ from country to country, there is a need to develop an efficient analytical method to identify the origin of the Angelica root. In order to obtain chemical fingerprints, three different Angelicae Radices were analyzed by direct analysis in real time mass spectrometry (DART-MS). Significantly different DART-MS spectra were observed from three different species of Angelicae Radix. Strong peaks of decursin or decusinol angelate, and its dimer were exclusively found from A. gigas. Ligustilide and linoleic acid were detected as the major component from A. acutiloba. The strongest ligustilide peak was observed from A. sinensis. DART-MS fingerprinting is a promising method for the rapid identification and/or quality control of Angelicae Radix.