• BMB Reports
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• v.56 no.2
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• pp.126-131
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• 2023

The abnormal accumulation and aggregation of the misfolded α-synuclein protein is the neuropathological hallmark of all α-synucleinopathies, including Parkinson's disease. The secreted proteins known as netrins (netrin-1, netrin-3, and netrin-4) are related to laminin and have a role in the molecular pathway for axon guidance and cell survival. Interestingly, only netrin-1 is significantly expressed in the substantia nigra (SN) of healthy adult brains and its expression inversely correlates with that of α-synuclein, which prompted us to look into the role of α-synuclein and netrin-1 molecular interaction in the future of dopaminergic neurons. Here, we showed that netrin-1 and α-synuclein directly interacted in pre-formed fibrils (PFFs) generation test, real time binding assay, and co-immunoprecipitation with neurotoxin treated cell lysates. Netrin-1 deficiency appeared to activate the dopaminergic neuronal cell death signal pathway via α-synuclein aggregation and hyperphosphorylation of α-synuclein S129. Taken together, netrin-1 can be a promising therapeutic molecule in Parkinson's disease.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.428-428
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• 2022

본 연구에서는 최근 RCM을 이용하여 생산된 미래 강우자료를 1시간강우량으로 변환하기 위한 Neyman-Scott Rectangular Pulse(NSRP) 모델 기반의 강우분해기법을 개발하고 이를 기반으로 짧은 지속시간에 대한 확률강우량이 어떻게 변화하는지 전망해보고자 하였다. 강우분해기법의 성능평가는 관측자료를 이용하여 수행되었으며, 관측 시계열을 우수하게 모의했으나 일최대 시간 강수량이 20mm를 초과하는 경우 불확실성이 증가함에 따라 사용에 주의가 필요할 것으로 판단된다. 미래 확률강우량 전망결과는 모든 지점(울산, 부산, 창원, 밀양)에서 향후 재현기간별 1시간 확률강우량이 증가될 것으로 전망되었다. 울산과 밀양 지점의 경우, 재현기간에 클수록 증가율 또한 증가하는 경향이 뚜렷하게 나타났는데 이는 상대적으로 복잡한 산악지역 내 위치하고 있고, 다른 지점보다 산지효과 영향이 크기 때문으로 판단된다. 부산과 창원지점은 다른 두 지점에 비해 재현 기간별 확률강우량의 변동성이 크게 나타났는데, 이는 해안에 가깝에 위치해 있어 RCM별 불확실성이 다소 크게 작용한 것으로 판단된다. 특히 과거 200년 빈도 확률강우량 보다 미래 50년미만 빈도 확률 강우량이 더 커질 수 있는 가능성을 확인하였다. 다양한 불확실성이 포함되어 있는 결과이긴 하나 이러한 결과를 기반으로 곧 도래할 미래의 도시유역 방재성능 재정비가 필요할 것으로 사료된다. 아울러, 극한 강우발생 가능성이 높아질 수 있음을 의미하기 때문에 이에 대한 새로운 수자원의 이수와 치수 대비를 위한 구조적/비구조적 대책이 시급할 것으로 판단된다.

• Electronics and Telecommunications Trends
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• v.39 no.1
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• pp.62-73
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• 2024

Artificial intelligence relies on data-driven analysis, and the data processing performance strongly depends on factors such as memory capacity, bandwidth, and latency. Fast and large-capacity memory can be achieved by composing numerous high-performance memory units connected via high-performance interconnects, such as Compute Express Link (CXL). CXL is designed to enable efficient communication between central processing units, memory, accelerators, storage, and other computing resources. By adopting CXL, a composable computing architecture can be implemented, enabling flexible server resource configuration using a pool of computing resources. Thus, manufacturers are actively developing hardware and software solutions to support CXL. We present a survey of the latest software for CXL memory utilization and the most recent CXL memory emulation software. The former supports efficient use of CXL memory, and the latter offers a development environment that allows developers to optimize their software for the hardware architecture before commercial release of CXL memory devices. Furthermore, we review key technologies for improving the performance of both the CXL memory pool and CXL-based composable computing architecture along with various use cases.

• Spatial Information Research
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• v.19 no.4
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• pp.81-89
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• 2011

This paper presents a multivariate kriging algorithm that integrates categorical data as secondary data for spatial interpolation of sparsely sampled ground survey data. Instead of using constant mean values in each attribute of categorical data, disaggregated local mean values at target grid points are first estimated by area-to-point kriging and then are used as local mean values in simple kriging with local means. This algorithm is illustrated through a case study of spatial interpolation of a geochemical copper element with geological map data. Cross validation results indicates that the presented algorithm leads to significant respective improvement of 15% and 25% in prediction capability, compared with univariate ordinary kriging and conventional simple kriging with constant mean values. It is expected that the multivariate kriging algorithm applied in this study would be effectively applied for spatial interpolation with categorical data.

• Applied Microscopy
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• v.14 no.2
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• pp.66-80
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• 1984

The organic phosphorus compounds have been widely used as an insecticide, since toxicity of these compounds is especially drastic to the insects than to men and other mammals. The organic phosphates are rapidly hydrolized and hence have little cumulative and ecologic effects. However, due to their acute toxic effects organophosphate have recorded rather high fatalities in men and domestic animals. The organic phosphorus compounds are powerful inhibitors to the carboxylic esterase enzymes such as acetylcholinesterase and pseudocholinesterase. As a result of firm binding characteristics of phosphate radicals to the active sites of enzyme, the activities of these enzymes are inhibited by the organophosphates. The organophosphates such as diazinon is easily observed from skin, gastrointestinal tract, conjunctivas and respiratory tract, and it is converted to more toxic form during metabolism in the liver The present study was carried out in order to investigate the hepatotoxicity of diazinon by observing the changes in the ultrastructure of cytoplasmic organelles of hepatic cells in albino mice. The animals were killed at 6, 12 and 24 hours after administration of 25mg/kg diazinon. The piece of hepatic tissue obtained from each animal was ultrathinly sectioned. The specimens stained by uranyl acetate and lead citrate double contrast methods were observed with JEM model 100B electron microscope. The results obtained were as follows: 1) A prominent dilatation and sacculation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane bound-ribosomes, and disaggregation of the free ribosomes were recognized. 2) The hypertrophy of the smooth endoplasmic reticulum associated with depletion of the glycogen particles was observed. 3) The atrophy of cisternae of Golgi complex was observed. 4) A large number of secondary lysosomes (autophagic vacuoles and residual bodies) were formed. Consequently it is suggested that diazinon would induce disorganization of the cytoplasmic organelles of hepatocytes in albino mice.

• Archives of Reconstructive Microsurgery
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• v.9 no.2
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• pp.179-185
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• 2000

In the field of microsurgery, the vascular obstruction of the microvascular anastomosis by thrombus is one of the most important complication. The purpose of this study is to compare the effect between aspirin and prostaglandin $E_1$($PGE_1$) which act as the peripheral vasodilatation and platelet disaggregation. We have used total 48 white male rats and divided them into three gruoups(A, B and C group). Each group consists of 16 rats respectively. A group is as control, B group is medicated with aspirin(3.0mg/kg/day) and C group with $PGE_1(1.2{\mu}g/kg/day)$. The gross and histopathologic findings at anastomosed site were observed on 3, 5, 10 and 15 days after vascular anastomosis and the results were obtained as the followings. 1. The microvascular patency rate is 81.2% in control group, 93.8% in aspirin group and 100% in $PGE_1$ group. 2. On the histologic examination, the formation of mural thrombus is decreased both in the aspirin and $PGE_1$ group as comparing with the control group and also the hypertrophy of the intima forming from media is less formed in $PGE_1$ group than aspirin group and the degree of thickeness is also less. 3. The fibrosis of media is less observed in $PGE_1$ group than aspirin group. According to the above results, the application of $PGE_1$ to the microsurgery is considered to be effective on the prevention of the thrombus formation and on providing high patency rate.

• Water for future
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• v.20 no.4
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• pp.257-266
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• 1987

The existing stochastic models for the data with hydrologic persistence can be classified into two categories; the short-term and long-term models.For the present study, the Hurst coefficients which are the dominant parameter in the Fast Fractional Gaussian Noise(FFGN)model, one of the long-term models. are estimated with historical annual and monthly streamflows. In order to verify the applicability of these estimators the statistical properties of the generated annual streamflows by FFGN model are compared with those of the historical annual streamflows. Then the generated annual streamflows by FFGN model are disaggregated into the monthly streamflows by disaggregation model at two sites, i.e. Waekman and Jindong, in the Nakdong River Basin. On the other hand, the monthly stream flows at the two sites were also generated by the two-site Matalas model which is one of the short-term models. To evaluate the applicability of the above models and to select the better model the statistical properties of the generated monthly streamflows by two models were compared with those of the historicals, respectively.

• Journal of Korean Society of Transportation
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• v.26 no.2
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• pp.147-156
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• 2008

It has been widely known that there are so many factors making travel demand errors in transportation forecasting steps. One of the reasons may stem from the level of aggregation of zone and network in analysis process. This paper investigates the effect of level of aggregation considering with number of zones in travel demand forecasting by expanding or reducing the zone and network gradually. Numerical results show that the aggregation could not make a significant impact on the travel demand, while disaggregation does. These results imply that a careful manipulation is required to add or to reduce zones and links in transportation planning process.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.696-703
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• 2014

Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-induced apoptosis, cytoskeletal disaggregation, cell detachment, and tight junction loss in cultured colonic epithelial cells. Serum samples were isolated from mice that survived a C. difficile infection following antibiotic treatment, and the antitoxin effects of these samples were investigated in toxin-A-exposed HT29 colonic epithelial cells and a toxin-A-induced animal model of gut inflammation. Unchallenged mice did not produce IgG against toxin A, whereas serum (antiserum) from C. difficile-challenged mice showed significant IgG responses against toxin A. Treatment with the antiserum markedly inhibited mucosal damage and inflammation in the toxin-A-treated mouse model. In contrast to control mouse serum, the antiserum also markedly inhibited toxin-A-induced DNA fragmentation, dephosphorylation of paxillin and Epo receptor (EpoR), deacetylation of tubulin, and upregulation of p21(WAF1/CIP1) and p53. Taken together, these results reveal that the generated antitoxin serum has biotherapeutic effects in preventing various C. difficile toxin-A-induced cellular toxicities.

• Journal of Korean Society on Water Environment
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• v.34 no.2
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• pp.210-215
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• 2018

It is difficult to count colonial cyanobacteria Microcystis cells since the thickness of colonies is constrained by amorphous mucilage, making it impossible to estimate the number of cells. Disaggregation of Microcystis colonies into single cell is needed to improve the accuracy and precision of cell density estimation of naturally collected samples. Uultrasonic treatment method is commonly used owing to the simplicity and immediacy of the procedure. However, amplitude, frequency, and duration of ultrasonic treatment also cause cell loss during the experiment. Optimal ultrasonic treatment has not been standardized yet. Therefore, the objective of this study was to investigate optimal ultrasonic treatment by analyzing cell density and colony numbers. We collected colonial Microcystis from Changnyeong-Haman weir area in Nakdong River during harmful algal boom period from September to October in 2017. Ultrasonic treatment method was applied to disrupt colonies into single cells to enumerate cell density. Among treatment conditions, results from continuously treated for 100 seconds were found to be the optimum to reduce colonies to a suspension of single cell without cell losses under high and low density of Microcystis cells. Lugol iodine fixed cells followed by sonication showed less negative impact of cell damage within the optimal treatment time (100 seconds). Furthermore, disaggregated cells treated by sonication enables microscopic observation more easily since gas vacuoles were collapsed to facilitate sedimentation of cells under the counting chamber for quantitative enumeration of buoyant Microcystis cells.