• Title/Summary/Keyword: Direction Vectors

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Screening of Promoters from Metagenomic DNA and Their Use for the Construction of Expression Vectors

  • Han, Sang-Soo;Lee, Jin-Young;Kim, Won-Ho;Shin, Hyun-Jae;Kim, Geun-Joong
    • Journal of Microbiology and Biotechnology
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    • v.18 no.10
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    • pp.1634-1640
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    • 2008
  • This study was focused on the screening of valuable genetic resources, such as promoters from metagenome, and describes a promoter trapping system with a bidirectional probe concept, which can select promoters or operons from various biological resources including metagenomic DNA. A pair of reporters, GFP and DsRed, facing the opposite direction without promoters, is an effective system that can function regardless of the direction of inserted promoters. The feasibility of this system was tested for the isolation of constitutively expressed promoters in E. coli from a soil metagenome, resulting in a potential pool of various promoters for practical application. The analyses of structural organization of the trapped genes demonstrated that constitutively expressible promoters in E. coli were broadly distributed within the metagenome, and suggested that some promoters were useful for the construction of expression vectors. Based on these observations, three constitutive promoters were employed in the expression vector system and their potentials for practical application were evaluated in terms of expression level, protein solubility, and effects on host growth.

A Spatial Error Concealment Technique Using Edge-Oriented Interpolation (방향성 보간을 이용한 공간적 에러 은닉 기법)

  • Yoo Hyun sun;Kim Won ki;Jeong Je chang
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.3C
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    • pp.133-140
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    • 2005
  • This paper introduces a spatial error concealment technique using directional interpolation in block-based compression. The first step involves finding the spatial direction vectors represented an edge-direction in the lost block using spatial boundary matching algorithm. Then, the error blocks are recovered by directional interpolation through these vectors and concealed by using the recovered blocks which have lower directional boundary matching error out of them relatively. This proposed method is able to deal with errors on macroblock or slice level adaptively. And it has lower complexity and maintains better performance compared to the conventional methods.

Optical Flow Measurement Based on Boolean Edge Detection and Hough Transform

  • Chang, Min-Hyuk;Kim, Il-Jung;Park, Jong an
    • International Journal of Control, Automation, and Systems
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    • v.1 no.1
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    • pp.119-126
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    • 2003
  • The problem of tracking moving objects in a video stream is discussed in this pa-per. We discussed the popular technique of optical flow for moving object detection. Optical flow finds the velocity vectors at each pixel in the entire video scene. However, optical flow based methods require complex computations and are sensitive to noise. In this paper, we proposed a new method based on the Hough transform and on voting accumulation for improving the accuracy and reducing the computation time. Further, we applied the Boo-lean based edge detector for edge detection. Edge detection and segmentation are used to extract the moving objects in the image sequences and reduce the computation time of the CHT. The Boolean based edge detector provides accurate and very thin edges. The difference of the two edge maps with thin edges gives better localization of moving objects. The simulation results show that the proposed method improves the accuracy of finding the optical flow vectors and more accurately extracts moving objects' information. The process of edge detection and segmentation accurately find the location and areas of the real moving objects, and hence extracting moving information is very easy and accurate. The Combinatorial Hough Transform and voting accumulation based optical flow measures optical flow vectors accurately. The direction of moving objects is also accurately measured.

Estimation of Sea Surface Current Vector based on Satellite Ocean Color Image around the Korean Marginal Sea

  • Kim, Eung;Ro, Young-Jae;Ahn, Yu-Hwan
    • Proceedings of the KSRS Conference
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    • v.2
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    • pp.816-819
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    • 2006
  • One of the most difficult parameters to measure in the sea is current speed and direction. Recently, efforts are being made to estimate the ocean current vectors by utilizing sequential satellite imageries. In this study, we attempted to estimated sea surface current vector (sscv) by using satellite ocean color imageries of SeaWifs around the Korean Peninsula. This ocean color image data has 1-day sampling interval and spatial resolution of 1x1 km. Maximum cross-correlation method is employed which is aimed to detect similar patterns between sequential images. The estimated current vectors are compared to the surface geostrophic current vectors obtained from altimeter of sea level height data. In utilizing the color imagery data, some limitations and drawbacks exist so that in warm water region where phytoplankton concentration is relatively lower than in cold water region, estimation of sscv is poor and unreliable. On the other hand, two current vector fields agree reasonably well in the Korean South Sea region where high concentration of chlorophyll-a and weak tide is observed. In the future, with ocean color images of shorter sampling interval by COMS satellite, the algorithm and methodology developed in the study would be useful in providing the information for the ocean current around Korean Peninsula.

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Error Prediction Considering the Measurement Direction in OMM System (OMM 시스템에서 측정방향을 고려한 가공물의 오차평가)

  • 최진필;이상조;권혁동
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.05a
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    • pp.632-635
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    • 2002
  • In this paper, a general procedure to determine machine tool errors from the on-machine measurement (OMM) data is described. First, a parameterized error model of a machine tool is illustrated by approximating error components as linear function of axis positions, and a modified error model is proposed which includes backlash effects. To determine the unknown model coefficient vectors of the forward and backward error model, an artifact with 8 cutes is made and calibrated on CMM. Then, lower-left and upper-right cube corners are measured with a touch-trigger probe mounted on the machine tool spindle. Measured error data are used to determine the coefficient vectors. The positioning errors in the XY plane at the fixed z position are simulated for the forward and backward error model.

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A new approach for content-based video retrieval

  • Kim, Nac-Woo;Lee, Byung-Tak;Koh, Jai-Sang;Song, Ho-Young
    • International Journal of Contents
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    • v.4 no.2
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    • pp.24-28
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    • 2008
  • In this paper, we propose a new approach for content-based video retrieval using non-parametric based motion classification in the shot-based video indexing structure. Our system proposed in this paper has supported the real-time video retrieval using spatio-temporal feature comparison by measuring the similarity between visual features and between motion features, respectively, after extracting representative frame and non-parametric motion information from shot-based video clips segmented by scene change detection method. The extraction of non-parametric based motion features, after the normalized motion vectors are created from an MPEG-compressed stream, is effectively fulfilled by discretizing each normalized motion vector into various angle bins, and by considering the mean, variance, and direction of motion vectors in these bins. To obtain visual feature in representative frame, we use the edge-based spatial descriptor. Experimental results show that our approach is superior to conventional methods with regard to the performance for video indexing and retrieval.

Smart Control System Using Fuzzy and Neural Network Prediction System

  • Kim, Tae Yeun;Bae, Sang Hyun
    • Journal of Integrative Natural Science
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    • v.12 no.4
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    • pp.105-115
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    • 2019
  • In this paper, a prediction system is proposed to control the brightness of smart street lamps by predicting the moving path through the reduction of consumption power and information of pedestrian's past moving direction while meeting the function of existing smart street lamps. The brightness of smart street lamps is adjusted by utilizing the walk tracking vector and soft hand-off characteristics obtained through the motion sensing sensor of smart street lamps. In addition, the motion vector is used to analyze and predict the pedestrian path, and the GPU is used for high-speed computation. Pedestrians were detected using adaptive Gaussian mixing, weighted difference imaging, and motion vectors, and motions of pedestrians were analyzed using the extracted motion vectors. The preprocessing process using linear interpolation is performed to improve the performance of the proposed prediction system. Fuzzy prediction system and neural network prediction system are designed in parallel to improve efficiency and rough set is used for error correction.

Low Power Scan Testing and Test Data Compression for System-On-a-Chip (System-On-a-Chip(SOC)에 대한 효율적인 테스트 데이터 압축 및 저전력 스캔 테스트)

  • 정준모;정정화
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.39 no.12
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    • pp.1045-1054
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    • 2002
  • We present a new low power scan testing and test data compression mothod lot System-On-a-Chip (SOC). The don't cares in unspecified scan vectors are mapped to binary values for low Power and encoded by adaptive encoding method for higher compression. Also, the scan-in direction of scan vectors is determined for low power. Experimental results for full - scanned versions of ISCAS 89 benchmark circuits show that the proposed method has both low power and higher compression.

Camera Motion Detection Using Estimation of Motion Vector's Angle (모션 벡터의 각도 성분 추정을 통한 카메라 움직임 검출)

  • Kim, Jae Ho;Lee, Jang Hoon;Jang, Soeun
    • Journal of Korea Multimedia Society
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    • v.21 no.9
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    • pp.1052-1061
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    • 2018
  • In this paper, we propose a new algorithm that is robust against the effects of objects that are relatively unaffected by camera motion and can accurately detect camera motion even in high resolution images. First, for more accurate camera motion detection, a global motion filter based on entropy of a motion vector is used to distinguish the background and the object. A block matching algorithm is used to find exact motion vectors. In addition, a matched filter with the angle of the ideal motion vector of each block is used. Motion vectors including 4 kinds of diagonal direction, zoom in, and zoom out are added additionally. The experiment shows that the precision, recall, and accuracy of camera motion detection compared to the recent results is improved by 12.5%, 8.6% and 9.5%, respectively.

Image Forgery Detection Using Gabor Filter (가보 필터를 이용한 이미지 위조 검출 기법)

  • NININAHAZWE, Sheilha;Rhee, Kyung-Hyune
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.520-522
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    • 2014
  • Due to the availability of easy-to-use and powerful image editing tools, the authentication of digital images cannot be taken for granted and it gives rise to non-intrusive forgery detection problem because all imaging devices do not embed watermark. Forgery detection plays an important role in this case. In this paper, an effective framework for passive-blind method for copy-move image forgery detection is proposed, based on Gabor filter which is robust to illumination, rotation invariant, robust to scale. For the detection, the suspicious image is selected and Gabor wavelet is applied from whole scale space and whole direction space. We will extract the mean and the standard deviation as the texture features and feature vectors. Finally, a distance is calculated between two textures feature vectors to determine the forgery, and the decision will be made based on that result.