• Title/Summary/Keyword: Direct method

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Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

  • Oh, Hee-Mock;Lee, Seog-June;Kim, Jee-Hwan;Park, Chan-Sun;Yoon, Byung-Dae
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.6
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    • pp.418-421
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    • 2000
  • A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

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Design of a Direct Adaptive Pole Placement Controller Without Persistency of Excitation (영구 여기 조건이 불필요한 직접 적응 극배치 제어기의 설계)

  • 신강욱;최홍규;박준열
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.17 no.10
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    • pp.1157-1163
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    • 1992
  • The development of adaptive control algorithms for possibly nonminimum phase systems has been hampered by singularities that may arise in the control law. To solve this problem, one securing convergence of the estimates to their true values by inducing persistency of excitation in the plant signals using direct adaptive control method and indirect adaptive control method, and another in which the estimates are adequately modified to meet the controllability requirements using indirect adaptive control method, without persistency of excitation. This paper presents an adaptive scheme that achieves regulation without persistent excitation condition using direct adaptive control method and reduces estimation algorithms with direct estimation of controller parameters without estimation of plant parameters.

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Using the PNF Approach to Improve Respiratory Function in Patients with Cervical Spinal Cord Injuries (고유수용성촉진법을 이용한 호흡운동이 경수 손상환자의 호흡기능에 미치는 영향 : 증례보고)

  • Song, Gui-Bin;Kim, Jung-Bin
    • PNF and Movement
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    • v.12 no.2
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    • pp.115-121
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    • 2014
  • Purpose: The study aimed to examine the influence of PNF direct and indirect breathing treatments for patients with cervical spinal cord injuries who had breathing problems. Methods: For each cervical spinal cord patient, force vital capacity (FVC), peak expiratory flow, maximum phonation time (MPT), rib cage width, and VAS were measured pre-intervention and four weeks after post-intervention. The indirect method and the direct method were used for interventions. We treated patients with the indirect method using scapular anterior depression pattern, bilateral extensor pattern with rhythmic initiation, and a combination of isotonic. We treated patients with the direct method, applying pressure on the sternum and using rhythmic initiation (hold relax and stretch reflex) for the rib cage. Training occurred for 50 minutes a day and three days per week for four weeks. Results: FVC, MPT, peak expiratory flow, and rib cage width were increased and decreased at the VAS point for rolling after treatment. Conclusion: Patients with cervical spinal cord injuries who had breathing problems felt uncomfortable when they had conversations on a couch. We found that PNF direct and indirect treatments improved rib cage width and breathing functions of patients with cervical spinal cord injuries.

RF Front-end Design of Direct Conversion Receiver using Six-Port (6-단자를 이용한 직접 변환 수신 전 처리부 설계)

  • Jang Myoung-shin;Kim Young-wan;Ko Nam-young
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.7
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    • pp.1534-1540
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    • 2005
  • The direct conversion method is classified into the structure using the mixing technology and six-port scheme. In the view point of complexity and integration the direct conversion method using the six-port scheme is superior to that with mixing technology. Expecially, the six-port direct conversion technology provides the low power consumption and the broad-band characteristic. In this paper, the six-port direct conversion receiver with the branch-line coupler and the ring hybrid coupler is desisted respectively. The performances of the designed six-port schemes are analyzed and the six-port scheme with superior performance characteristics is proposed.

Evaluation of interface shear strength between geosynthetics using three kinds of testing methods (다양한 시험법에 의한 토목섬유 사이의 접촉 전단 강도 평가)

  • Seo, Min-Woo;Park, Jun-Boum;Park, Inn-Jun
    • Proceedings of the Korean Geotechical Society Conference
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    • 2004.03b
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    • pp.356-363
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    • 2004
  • In this research, the shear behavior of four different interfaces consisting of 4 types of geosynthetics was examined, and both static and dynamic tests for the geosynthetic interface were conducted. The monotonic shear experiments were performed by using an inclined board apparatus and large direct shear device. The interface shear strength obtained from the inclined board tests were compared with those calculated from large direct shear tests. The comparison results indicated that direct shear tests are likely to overestimate the shear strength in low normal stress range where direct shear tests were not performed. Curved failure envelopes were also obtained for interface cases where two static shear tests were conducted. By comparing the friction angles measured from three tests, i.e. direct shear, inclined board, and shaking table tests, it was found that the friction angle might be different depending on the test method and normal stresses applied in this research. Therefore, it was concluded that the testing method should be determined carefully by considering the type of loads and the normal stress expected in the field.

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RF Front-end Design of Direct Conversion Receiver using Six-Port (6-단자를 이용한 직접 변환 수신 전 처리부 설계)

  • Jang, Myoung-Shin;Yang, Woo-Jin;Oh, Dae-Ho;Kim, Young-Wan;Ko, Nam-Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.2
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    • pp.481-484
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    • 2005
  • The direct conversion method is classified into the structure using the mixing technology and six-port scheme. In the view point of complexity and integration the direct conversion method using the six-port scheme is superior to that with mixing technology. Expecially, the six-port direct conversion technology provides the low power consumption and the broad-band characteristic. In this paper, the six-port direct conversion receiver with the branch-line coupler and the ring hybrid coupler is designed respectively. The performances of the designed six-port schemes are analyzed and the six-port scheme with superior performance characteristics is proposed.

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A Modified Method for the Boundary Fitted Coordinate Systems to Analysis of Gas Bearings Considering Upstream In Extremely High Compressibility Number Region

  • Khan, Polina;Hwang, Pyung;Park, Sang-Shin
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.10b
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    • pp.105-106
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    • 2002
  • An expanded scheme of direct numerical solution method for solving the Reynolds' equation in the boundary fitted coordinate systems for the gas lubrication with ultra low clearance is presented. Skewed slider is calculated by this scheme and results are compared to the original direct numerical solution. The modified scheme has advantages in stability in high compressibility number region. At the lower A region the difference in results of original and modified method is several percents.

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Depth Control of Underwater Glider by Lyapunov's Direct Method (리야푸노프 직접법에 의한 수중 글라이더의 깊이 제어)

  • Joo, Moon Gab
    • IEMEK Journal of Embedded Systems and Applications
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    • v.12 no.2
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    • pp.105-112
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    • 2017
  • To control the depth of an underwater glider, a control method by using Lyapunov's direct method is proposed. The underwater glider has a torpedo-shape hull, a movable mass in the hull, and an inflatable buoyancy bag in the hull, but doesn't have large wings that increase the lift force for the conventional underwater glider. The control laws to adjust the position of the movable mass and the mass of the inflatable buoyancy bag are derived. For a selected speed and an angle of attack, we simulated the operation of the underwater glider using Matlab/Simulink. The efficiency of the proposed controller is shown in the fact that the control effort is active during only a short period of time when the zigzag trajectory is changed from downward to upward or vice versa.

Three Dimensional Finite Element Inverse Analysis of Rectangular Cup and S-Rail Forming Processes using a Direct Mesh Mapping Method (직접 격자 사상법을 이용한 직사각컵 및 S-Rail 성형공정의 3차원 유한요소 역해석)

  • Kim S. H.;Huh H.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2001.10a
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    • pp.81-84
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    • 2001
  • An inverse finite element approach is employed for more capability to design the optimum blank shape from the desired final shape with small amount of computation time and effort. In some drawing or stamping simulation with inverse method, it is difficult to apply inverse scheme due to the large aspect ratio or steep vertical angle of inclination. The reason is that initial guesses are hard to make out with present method for those cases. In this paper, a direct mesh marring scheme to generate initial guess on the sliding constraint surface described by finite element patches is suggested for one step inverse analysis to calculate initial blank shape. Radial type mapping is adopted for the simulation of rectangular cup drawing process with large aspect ratio and parallel type mapping for the simulation of S-Rail forming process with steep vertical angle of inclination.

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Development of Direct Surface Forming Process

  • Cho, Kwang-Hwan;Yoon, Kyung-Hwan
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.04a
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    • pp.73-77
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    • 2003
  • The backlight unit(BLU) is used as a light source of TFT liquid-crystalline-display (TFT-LCD) module. In this backlight unit, one of important components is the light guide, which is usually made of transparent polymers. Currently the screen-printing method is mainly used for the light guide as a manufacturing process. However, it has limitation to the flexibility of three-dimensional optical design. In the present paper a new alternative manufacturing method for the light guide with low-cost is proposed. This manufacturing method is named as direct surface forming (DSF), which is very similar to the well-known hot embossing except for partial contact between mold and substrate. The results of this new manufacturing method are presented in terms of processing condition, dimensional accuracy, productivity, etc.

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