• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 추계학술대회논문집
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• pp.394-400
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• 2008

This paper presents an analytical method to investigate the stability of FDBs (fluid dynamic bearings) considering the tilting motion. The perturbed equations of motion are derived with respect to translational and tilting motion for the general rotor-bearing system with five degrees of freedom. The Reynolds equations and their perturbed equations are solved by using the FEM in order to calculate the pressure, load capacity, and the stiffness and damping coefficients. This research introduces the radius of gyration to the equations of notion in order to express the mass moment of interia with respect to the critical mass. Then the critical mass of FDBs is determined by solving the eigenvalue problem of the linear equations of motion. This research is numerically validated by comparing the stability chart of FDBs with the time response of the whirl radius obtained from the direct integration of the equations of motion. This research shows that the tilting motion is one of the major design considerations to determine the stability of rotating system. It also shows that the stability of FDBs considering only translation is overestimated in comparison with the stability of FDBs considering both translational and tilting motion.

• Journal of Microbiology and Biotechnology
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• 제13권5호
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• pp.823-827
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• 2003

Streptomyces venezuelae ATCC 15439 is notable in its ability to produce two distinct groups of macrolactones. It has been reported that the generation of two macrolactone structures results from alternative expression of pikromycin (Pik) polyketide synthase (PKS). It was previously reported that the hybrid pikromycin-tylosin PKS can also produce two different macrolactones but its mechanistic basis remains unclear. In order to address this question, a series of site-directed mutagenesis of tentative alternative ribosome binding site and translation start codons in tylGV were performed. The results suggest that macrolactone ring size is not determined by the alternative expression of TylGV but through other mechanism(s) involving direct interaction between the PikAIII and TE domain or skipping of the final chain elongation step. This provides new insight into the mechanism of macrolactone ring size determination in hybrid PKS as well as an opportunity to develop novel termination activities for combinatorial biosynthesis.

• Geomechanics and Engineering
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• 제16권2호
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• pp.205-215
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• 2018

Strain-based criteria are known as a direct method in determining the stability of the geomechanical structures. In spite of the widely use of Sakurai critical strain criterion, it is so conservative to make use of them in rocks with initial plastic deformation on account of the considerable difference between the failure and critical strains. In this study, a new criterion has been developed on the basis of the failure strain to attain more reasonable results in determining the stability status of the tunnels excavated in the rocks mostly characterized by plastic-elastic/plastic behavior. Firstly, the stress-strain curve was obtained having conducted uniaxial compression strength tests on 91 samples of eight rock types. Then, the initial plastic deformation was omitted making use of axis translation technique and the criterion was presented allowing for the modified secant modulus and by use of the failure strain. The results depicted that the use of failure strain criterion in such rocks not only decreases the conservativeness of the critical strain criterion up to 42%, but also it determines the stability status of the tunnel more accurately.

• 한국지능시스템학회논문지
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• 제21권2호
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• pp.276-282
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• 2011

CoreNet은 한-중-일 다국어 텍스트의 분석, 언어 간 변환을 포함한 자연어처리에 유용한 자원이다. CoreNet의 보다 광범위한 분야 및 응용에의 활용을 장려하고 다국어 어휘의미망으로서의 국제적 위상을 제고하기 위해 SUMO에 연결하는 작업을 하였다. CoreNet과 SUMO를 매핑하기 위해 간접 매핑과 직접 매핑 방법을 모두 사용하였는데, CoreNet-KorLex-PWN-SUMO에 이르는 간접 매핑 작업을 통하여 한국어 중심의 CoreNet과 영어로 기술된 SUMO의 언어 간 변환의 어려움을 완화하고 CoreNet 개념에 대응하는 SUMO 클래스의 재현율을 극대화하였다.

• Journal of Ginseng Research
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• 제41권4호
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• pp.469-476
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• 2017

Background: Panax ginseng Meyer (Asian ginseng) has a large nuclear genome size of > 3.5 Gbp in haploid genome equivalent of 24 chromosomes. Tandem repeats (TRs) occupy significant portions of the genome in many plants and are often found in specific genomic loci, making them a valuable molecular cytogenetic tool in discriminating chromosomes. In an effort to understand the P. ginseng genome structure, we characterized an ultrahigh copy 167-bp TR (Pg167TR) and explored its chromosomal distribution as well as its utility for chromosome identification. Methods: Polymerase chain reaction amplicons of Pg167TR were labeled, along with 5S and 45S rDNA amplicons, using a direct nick-translation method. Direct fluorescence in situ hybridization (FISH) was used to analyze the chromosomal distribution of Pg167TR. Results: Recently, we reported a method of karyotyping the 24 chromosome pairs of P. ginseng using rDNA and DAPI (4',6-diamidino-2-phenylindole) bands. Here, a unique distribution of Pg167TR in all 24 P. ginseng chromosomes was observed, allowing easy identification of individual homologous chromosomes. Additionally, direct labeling of 5S and 45S rDNA probes allowed the identification of two additional 5S rDNA loci not previously reported, enabling the refinement of the P. ginseng karyotype. Conclusion: Identification of individual P. ginseng chromosomes was achieved using Pg167TR-FISH. Chromosome identification is important in understanding the P. ginseng genome structure, and our method will be useful for future integration of genetic linkage maps and genome scaffold anchoring. Additionally, it is a good tool for comparative studies with related species in efforts to understand the evolution of P. ginseng.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2009년 추계학술대회논문집
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• pp.151-158
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• 2009

To implement the insects' flapping flight for developing flapping MAVs(micro air vehicles), the unsteady flow characteristics of the insects' forward flight is investigated. In this paper, two-dimensional FSI(Fluid-Structure Interaction) simulations are conducted to examine realistic flow features of insects' flapping flight and to examine the flexibility effects of the insect's wing. The unsteady incompressible Navier-Stokes equations with an artificial compressibility method are implemented as the fluid module while the dynamic finite element equations using a direct integration method are employed as the solid module. In order to exchange physical information to each module, the common refinement method is employed as the data transfer method. Also, a simple and efficient dynamic grid deformation technique based on Delaunay graph mapping is used to deform computational grids. Compared to the earlier researches of two-dimensional rigid wing simulations, key physical phenomena and flow patterns such as vortex pairing and vortex staying can still be observed. For example, lift is mainly generated during downstroke motion by high effective angle of attack caused by translation and lagging motion. A large amount of thrust is generated abruptly at the end of upstroke motion. However, the quantitative aspect of flow field is somewhat different. A flexible wing generates more thrust but less lift than a rigid wing. This is because the net force acting on wing surface is split into two directions due to structural flexibility. As a consequence, thrust and propulsive efficiency was enhanced considerably compared to a rigid wing. From these numerical simulations, it is seen that the wing flexibility yields a significant impact on aerodynamic characteristics.

• 한국통신학회논문지
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• 제32권1B호
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• pp.61-70
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• 2007

IPv4/IPv6 통신 서비스를 위한 변환기는 호스트의 변경 없이 IPv6 호스트와 IPv4 호스트간의 통신을 지원해 준다. 그러나 호스트가 모바일 노드일 경우 삼각형 라우팅 문제가 발생한다. Mobile IPv4 모바일 노드가 이동할 경우 홈 에이전트와 IPv6의 CN(Correspondent Node)에게 반드시 이동 정보를 보내 주어야 하지만 현재 NAT-PT는 IPv4와 IPv6의 이동성 헤더인 모바일 IPv4/Mobile IPv6의 헤더 변환은 지원해 주지 않기 때문에 삼각형 라우팅 문제가 발생하여 Mobile IPv4와 Mobile IPv6간에 비효율적인 통신을 일으킨다. 본 논문에서는 Mobile IPv4와 Mobile IPv6 통신에서 발생하는 삼각형 라우팅 문제를 해결하기 위한 모바일 헤더 변환 메커니즘을 제안하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2287-2290
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• 2014

Background: In the past few years, a considerable number of preclinical studies have been proposed metformin as a potential anticancer agent, but some of these studies suffer from a number of methodological limitations such as assessment of cytotoxicity in the presence of supraphysiological glucose concentrations or applying suprapharmacological levels of the drug. These objections have limited the translation of published preclinical data to the clinical setting. The present study aimed to investigate direct anticancer effects of metformin on different molecular subtypes of breast cancer with pharmacological concentrations and under normoglycemic conditions in vitro. Materials and Methods: Breast cancer cell lines from luminal A, luminal B, ErbB2 and triple-negative molecular subtypes were treated with a pharmacological concentration of metformin (2mM) at a glucose concentration of 5.5mM. Time-dependant cell viability was assessed by dye exclusion assay. MTTbased cytotoxicity assays were also performed with metformin alone or in combination with paclitaxel. Results: Metformin did not show any growth inhibitory effects or time-dependant cytotoxicity on breast cancer cell lines in the presence of normal glucose concentrations at the therapeutic plasma level. No augmentation of the antineoplastic properties of paclitaxel was apparent under the tested conditions. Conclusions: Metformin is probably unable to exert cytotoxic or cytostatic effects on breast cancer subtypes at pharmacological concentrations and normal plasma glucose levels. These results highlight the importance of establishing a higher steady-state plasma concentration of metformin in the clinical setting for assessment of anticancer effects in normoglycemic patients.

• 대한치과교정학회지
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• 제45권1호
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• pp.20-28
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• 2015

Objective: The purpose of this study was to observe stress distribution and displacement patterns of the entire maxillary arch with regard to distalizing force vectors applied from interdental miniscrews. Methods: A standard three-dimensional finite element model was constructed to simulate the maxillary teeth, periodontal ligament, and alveolar process. The displacement of each tooth was calculated on x, y, and z axes, and the von Mises stress distribution was visualized using color-coded scales. Results: A single distalizing force at the archwire level induced lingual inclination of the anterior segment, and slight intrusive distal tipping of the posterior segment. In contrast, force at the high level of the retraction hook resulted in lingual root movement of the anterior segment, and extrusive distal translation of the posterior segment. As the force application point was located posteriorly along the archwire, the likelihood of extrusive lingual inclination of the anterior segment increased, and the vertical component of the force led to intrusion and buccal tipping of the posterior segment. Rotation of the occlusal plane was dependent on the relationship between the line of force and the possible center of resistance of the entire arch. Conclusions: Displacement of the entire arch may be dictated by a direct relationship between the center of resistance of the whole arch and the line of action generated between the miniscrews and force application points at the archwire, which makes the total arch movement highly predictable.

• The Plant Pathology Journal
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• 제40권2호
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• pp.106-114
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• 2024

Fusarium head blight (FHB), predominantly caused by Fusarium graminearum and F. asiaticum, is a significant fungal disease impacting small-grain cereals. The absence of highly resistant cultivars underscores the need for vigilant FHB surveillance to mitigate its detrimental effects. In 2023, a notable FHB outbreak occurred in the southern region of Korea. We assessed FHB disease severity by quantifying infected spikelets and grains. Isolating fungal pathogens from infected samples often encounters interference from various microorganisms. We developed a cost-effective, selective medium, named BGT (Burkholderia glumae Toxoflavin) medium, utilizing B. glumae, which is primarily known for causing bacterial panicle blight in rice. This medium exhibited selective growth properties, predominantly supporting Fusarium spp., while substantially inhibiting the growth of other fungi. Using the BGT medium, we isolated F. graminearum and F. asiaticum from infected wheat and barley samples across Korea. To further streamline the process, we used a direct PCR approach to amplify the translation elongation factor 1-α (TEF-1α) region without a separate genomic DNA extraction step. Phylogenetic analysis of the TEF-1α region revealed that the majority of the isolates were identified as F. asiaticum. Our results demonstrate that BGT medium is an effective tool for FHB diagnosis and Fusarium strain isolation.