• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.323-327
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• 2016

A man with only yellowing of the skin and eye sclera was diagnosed with clonorchiasis, which rarely manifested jaundice as the initial symptom. However, because of a lack of evidence for a diagnostic gold standard, the time until definitive diagnosis was more than a week. The diagnostic process relied on inquiring about the patient's history, including the place of residence, dietary habits, and symptoms, as well as on serological findings, an imaging examination, and pathological findings. MRCP and CT results showed mild dilatation of intrahepatic ducts and increased periductal echogenicity. The eggs were ultimately found in stool by water sedimentation method after the negative report through direct smear. DNA sequencing of PCR production of the eggs demonstrated 98-100% homology with ITS2 of Clonorchis sinensis. After anti-parasite medical treatment, the patient's symptoms were gradually relieved. Throughout the diagnostic procedure, besides routine examinations, the sedimentation method or concentration method could be used as a sensitive way for both light and heavy C. sinensis infection in the definite diagnosis.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6269-6273
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• 2014

Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.479-483
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• 2012

The secretory efficiency of recombinant xylanase xynB from yeast Pichia pastoris between the ${\alpha}$-factor preprosequence and a classical mammalian signal peptide derived from bovine ${\beta}$-casein was compared. The results showed that although the bovine ${\beta}$-casein signal peptide could direct high-level secretion of recombinant xylanase, it was relatively less efficient than the ${\alpha}$-factor preprosequence. In contrast, the bovine ${\beta}$-casein signal peptide caused remarkably more recombinant xylanase trapped intracellularly. Real-time RT-PCR analysis indicated that the difference in the secretory level between the two signal sequences was not due to the difference in the transcriptional efficiency.

• 한국응급구조학회지
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• 제9권1호
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• pp.101-109
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• 2005

The purpose of this study which was done by 250 Prehospital Care Reports(PCRs) survey of some squads in Seoul Metropolitan Fire & Disaster Management Department was to improve prehospital emergency care by means of quality management. The data were collected in 3 squads from Jun. 21 to Jul. 18, 2004 and analyzed by using SPSS Win 12.0 Version. The conclusions from this study were summarized as follows. The mean time of Event to treatment interval was $4.6{\pm}4.3$ minutes and 49.2% arrived at patient within 4 minutes. Platinum minute was observed 61.1% of verbal response, 73.3% of painful response, 77.8% of unresponsive. The great majority of patients couldn't receive advanced life support on account of limited scope of practice and strict direct medical control in the Emergency Medical Services Act. Data from quality improvement activity will be useful to expand indirect medical control which is able to activate prehospital care. To utilize PCR for quality improvement. It has to have data elements, run data, patient data, check boxes, narrative including US DOT's minimum data set.

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.309-312
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• 2019

Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.

• The Plant Pathology Journal
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• 제23권4호
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• pp.260-265
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• 2007

Testing a large number of samples from field monitoring and routine indexing is cumbersome and the available virus detection tools were labor intensive and expensive. To circumvent these problems we established tissue blot immunoassay (TBIA) method an alternative detection tool to detect Barley mild mosaic virus (BaMMV) and Barley yellow mosaic virus (BaYMV) infection in the field and greenhouse inoculated plants for monitoring and routine indexing applications, respectively. Initially, leaf and stem were tested to determine suitable plant tissue for direct blotting on nitrocellulose membrane. The dilutions of antibodies were optimized for more efficient and economical purposes. Results showed that stem tissue was more suitable for direct blotting for it had no background that interferes in the reaction. Therefore, this technique was referred as direct stem blot immunoassay or DSBIA, in this study. Re-used diluted (1:1000) antiserum and conjugate up to 3 times with the addition of half strength amount of concentrated antibodies was more effective in detecting the virus. The virus blotted on the nitrocellulose membrane from stem tissues kept at room temperature for 3 days were still detectable. The efficiency of DSBIA and RT-PCR in detecting BaMMV and BaYMV were relatively comparable. Results further proved that DSBIA is a rapid, reliable and economical detection method suitable for monitoring BaMMV and BaYMV infection in the field and practical method in indexing large scale of barley materials for virus resistance screening.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.960-966
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• 2012

The diversity and composition of yeast populations may greatly impact wine quality. This study investigated the yeast microbiota in two different types of wine fermentations: direct inoculation of a commercial starter versus pied de cuve method at an industrial scale. The pied de cuve fermentation entailed growth of the commercial inoculum used in the direct inoculation fermentation for further inoculation of additional fermentations. Yeast isolates were collected from different stages of wine fermentation and identified to the species level using Wallersterin Laboratory nutrient (WLN) agar followed by analysis of the 26S rDNA D1/D2 domain. Genetic characteristics of the Saccharomyces cerevisiae strains were assessed by a rapid PCR-based method, relying on the amplification of interdelta sequences. A total of 412 yeast colonies were obtained from all fermentations and eight different WL morphotypes were observed. Non-Saccharomyces yeast mainly appeared in the grape must and at the early stages of wine fermentation. S. cerevisiae was the dominant yeast species using both fermentation techniques. Seven distinguishing interdelta sequence patterns were found among S. cerevisiae strains, and the inoculated commercial starter, AWRI 796, dominated all stages in both direct inoculation and pied de cuve fermentations. This study revealed that S. cerevisiae was the dominant species and an inoculated starter could dominate fermentations with the pied de cuve method under controlled conditions.

• 생명과학회지
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• 제19권9호
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• pp.1328-1332
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• 2009

고래회충유충증은 해산어류에 기생하는 고래회충과(family Anisakidae)에 속하는 선충류 유충에 의한 질병으로 유충의 직접적인 위장관내 침입으로 인한 병변과 더불어 유충의 분비 배설물에 의한 알레르기 질환도 유발될 수 있다. 고래회충유충증은 A. simplex를 비롯하여 Contracaecum, Pseudoterranova, Hysterothylacium 등의 유충에 의해 야기될 수 있으나 이들에 대한 형태학적 감별 진단은 유충의 형태적 유사성으로 인하여 매우 어려운 경우가 많다. 이러한 형태학적 진단의 어려움을 극복하고 분자생물학적 감별진단 방법을 확립하기 위하여 A. simplex, Contracaecum type A. type C' 및 Goezia 유충을 숭어, 도다리, 고등어, 아나고, 참돔 등 5종의 어류에서 분리하였다. 각각의 유충으로부터 분리한 18S rDNA를 PCR로 증폭한 후 Taq 1, Hinf I, Hha I, Alu 1, Dde I, Hae III, Sau 96I, Sau 3AI 등 8종의 제한효소를 사용하여 PCR-RFLP를 시행하였다. PCR product의 크기는 약 2.0 Kb였으며 Hinf l, Alu 1, Hha I, Dde 1 및 Hae III로 A. simplex와 Contracaecum type C'을 구분할 수 있었다. 그러나 Contracaecum type A의 경우에는 Taq I, Hinf I, Alu I 및 Dde I의 경우에는 2가지 패턴으로 나타났으며 이들 가운데 일부는 A. simplex, Contracaecum type C', 및 Goezia와 동일한 분석 패턴을 보이기도 하였다. Goezia는 사용한 8개의 제한 효소 모두에서 A. simplex 및 Contracaecum type A 및 type C'과 각기 다른 양상을 보였다. 이러한 결과로 18S rDNA PCR-RFLP 방법은 A. simplex와 Contracaecum type C'의 감별 진단에 유용한 것으로 밝혀졌으며, Contracaecum type A의 분류에는 제한적으로 사용되어야 함은 물론 형태학적 분류 기준에 대한 재검토가 뒤따라야 할 것으로 사료되었다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제4권2호
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• pp.213-217
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• 2001

목적: 당원병 Ia형(von Gierke disease)은 glucose 6-phosphatase (G6Pase)의 결함으로 인하여 출생 시부터 복부팽만과 간종대가 나타나고 저혈당, 락트 산혈증(lactic acidemia), 고지방혈증(hyperlipidemia), 고뇨산혈증(hyperuricemia) 등을 초래하며 점차 성장발육부전이 진행되는 상염색체 열성 유전성 질환이다. 본 연구에서는 한국인 GSD Ia 환자 9명을 대상으로 G6Pase 유전자의 돌연변이형에 관한 분석을 처음으로 시행하고자 하였다. 방법: 임상적인 증상과 G6Pase 효소 측정결과를 통하여 GSD Ia로 진단된 9명 환자의 혈액에서 genomic DNA를 분리하여 G6Pase 유전자의 5개 exon 부분이 포함되도록 polymerase chain reaction(PCR) 한 후 PCR 산물을 direct sequencing 하였다. 결과: 9명의 GSD Ia 환자 중 7명 환자에서 g727t homozygote 돌연변이를 발견하였고, 한 환자는 heterozygote로서 g727t 돌연변이와 c611g 돌연변이가 발견되었다. 나머지 한 명에서는 g727t 돌연변이 하나만 발견할 수 있었다. 한 개의 allele에서 발견된 c611g 돌연변이는 exon 4의 178번 아미노산 proline이 alanine으로 변경되는 것으로 지금까지 보고된 바 없는 새로운 돌연변이이다. 결론: 현재까지는 GSD Ia병의 정확한 진단을 위해서 환자의 간 생검 조직에서 G6Pase 효소의 활성도를 측정하는 것이 필요하였으나 한국인 GSD Ia 환자의 경우 g727t 돌연변이가 대부분을 차지하고 있기 때문에 앞으로는 상당수의 환자에서 혈액 채취만으로도 G6Pase 유전자 분석을 통한 GSD 진단이 손쉽게 이루어질 수 있으리라고 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10387-10391
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• 2015

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.