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Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China

  • He, Li (Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University) ;
  • Fang, Zheng-Ming (Department of Parasitology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Xue, Ting (Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University) ;
  • Zhang, Er-Fu (Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University) ;
  • An, Chun-Li (Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University)
  • Received : 2018.08.09
  • Accepted : 2019.04.28
  • Published : 2019.06.30

Abstract

Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.

Keywords

References

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