• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.179-186
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• 2005

In oriental medicine, Achyranthis Bidentatae Radix & Dipsaci Radix are frequently used materials for strenthing lumbar and knees. Bang-Yak-Hap-Pyun(方藥合編) is widely used for clinical herbal prescription book. In Bang-Yak-Hap-Pyun, Achyranthis Bidentatae Radix is used 1g~8g. and Dipsaci Radix is used 1g~3.2g. The most important fact is that the prescription used chyranthis Bidentatae Radix & Dipsaci Radix at the same time is only 1(Man-Geum-Tang-the ratio of Achyranthis Bidentatae Radix & Dipsaci Radix is 1:1). The number of prescription Achyranthis Bidentatae Radix & Dipsaci Radix in Bang-Yak-Hap-Pyun is total 19. 14 in sang-tong(上統), 2 in jung-tong(中統), 3 in ha-tong(下統). The group of mostly used disease is about lumbar and knees. The ratio of Achyranthis Bidentatae Radix in Bang-Yak-Hap-Pyun is 3.88%~25%. And the ratio of Dipsaci Radix is 8.33%~12.5%. The mostly quoted medical literature what prescription included Achyranthis Bidentatae Radix & Dipsaci Radix in Bang-Yak-Hap-Pyun is Dong-Yi-Bo-Gam and Gyung-Ak-Jun-Su Sin-Bang-Pal-Jin.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.159-168
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• 2008

Objectives : The present study has been undertaken to investigate the effects of Dipsaci Radix on Muscle Fiber Atrophy and MyoD Expression in Gastrocnemius of MCAO Rats Methods : In order to investigate effects of Dipsaci radix on the skeletal muscle atrophy following stroke, cerebral infarct was induced by the middle cerebral artery occlusion (MCAO) in the rats. Water extract of Dipsaci radix (184.4 mg/100 g) was treated for 4 weeks, once a day orally, after the MCAO. Effects were evaluated with muscle fiber type composition and cross-sectioned area of muscle fibers in gastrocnemius of the unaffected & affected hind limbs. And MyoD protein expression in gastrocnemius was demonstrated with immunohistochemistry and western blotting. Results : Obtained results were as follows; 1. Infarct volume was not attenuated by Dipsaci radix treatment in the MCAO rats. 2. At the affected-side hind limb of the MCAO rats, the increase of type-I fibers and the decrease of type-II fibers were induced by Dipsaci radix treatment. 3. At the affected-side hind limb of the MCAO rats, decreases of cross-sectioned areas of type-I and type-II fibers were attenuated by Dipsaci radix treatment. 4. At the affected-side hind limb of the MCAO rats, MyoD positive cells were increased by Dipsaci radix treatment. 5. At the affected-side hind limb of the MCAO rats, MyoD expressions were increased by Dipsaci radix treatment. Conclusions : These results suggest that Dipsaci radix has a protective effect against muscle atrophy, through the inhibition of the muscle cell apoptosis, following the central nervous system demage.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.678-684
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• 2009

It was reported that Dipsaci Radix has decrease pain effect on the Complex Region Pain Syndrome(CRPS). the CRPS was induced by unilateral loose occlusion in 4 part of the sciatic nerve of the rats. For the fingding significantly change on CRPS rats were divided into 4 different experimental groups. and each groups were induced CRPS. Experimental group I (control group; n=15), experimental group II (100 mg/kg Dipsaci Radix dieted rats; n=15), experimental group III (300 mg/kg Dipsaci Radix dieted rats; n=15), and experimental group IV(500 mg/kg Dipsaci Radix dieted rats; n=15). The study of Dipsaci Radix concentration was that foot withdrawal threshold to the thermal stimuli(Hot plate test), foot withdrawal threshold to the mechanical stimuli(von Frey's filament) and immunohistochemistry staining that were substance P. Hot plate test and von Frey Filament were increase in experimental group II, III, IV than group I, especially group III was most significantly change than group II and IV in post-hoc(Duncan's multiple range). and In immunohistochemistry observation; group I showed increase in the group II, III, IV. especially group III had the minimal level of the substance P expression while the experimental group II, III. These results suggested that the Dipsaci Radix dieted made the decrease of pain in CRPS.

• Korean Journal of Pharmacognosy
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• v.43 no.2
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• pp.137-146
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• 2012

Dipsaci Radix (Dipsacaceae) has been used as a tonic, an analgesic, anti-inflammatory and anti-complement agents in traditional herbal medicine for the therapy of low back pain, knee pain, rheumatic arthritis, traumatic hematoma, and bone fractures. A high-performance liquid chromatography-electrospray ionization-mass spectrometric method (HPLC-ESI-MS) was developed for the simultaneous quantitation method of the five compounds from the herbal drug: asperosaponin VI and asperosaponin XII (terpene glycosides), sweroside, loganin and dipsacus A(iridoid glycosides). HPLC separation of the analytes was achieved on a C18 column ($150{\times}2.0$ mm i.d., 5 ${\mu}m$) using the aqueous methanol containing 5 mM ammonium acetate with gradient flow of the mobile phase. Detection of the analytes was performed by positive ion electrospray ionization, and selected ion monitoring was used for data acquisition using m/z corresponding molecular adduct ion, $[M+NH_4]^+$ and $[M+H]^+$. Calibration graphs showed good linearity ($r^2$=0.9997) over the wide range of the analytes; intra- and inter-day precisions (RSD, %) were within 9.1% and the accuracy between 94.0-111.0%. Recoveries of the analytes through the assay procedure were in the range of 93.7-110.8%. Analytical results of the herbal drugs of Dipsaci Radix (17 samples) show wide distribution of the five marker compounds and clear difference of the species from Phlomidis Radix (4 samples). The developed method would provide a practical guide for the quality control of the herbal drug.

• Korean Journal of Oriental Medicine
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• v.13 no.1 s.19
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• pp.147-152
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• 2007

As a result to amplifying 12 samples of 'Sok-dan' through an random amplified polymorphic DNA (RAPD) method using eighteen DEC and URP primers, distinct band forms enabling discrimination of Phlomus umbrosa and Dipsacus asperoides were observable in the UBC 320 primer, UBC 367 primer, UBC 385 primer, UBC 414 primer, UBC 423 primer, URP 3 primer, URP 5 primer and URP 9 primer. The polymorph result amplified with a random primer was evaluated through Gelcompar II, showing a result dividable into two groups. The divided groups were the dried sample group of Dipsacus asperoides and the group of Phlomis umbrosa. In order to recognize the distinction between Dipsaci Radix types, the genetic variation of 'Sok-dan' produced domestically and imported was evaluated through RAPD, and the potential to distinguish these in forms of dried medicine was identified, presenting a method to authentification of Phlomis umbrosa and Dispacus asperoides.

• Korean Journal of Pharmacognosy
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• v.43 no.1
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• pp.79-84
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• 2012

A simple and reliable reverse phase HPLC method was developed to determine pharmacologically active marker compounds of Dipsaci Radix, Leonuri Herba and Cyperi Rhizoma. The stability test of water-extract of three natural medicines were examined for six months. However, no significant change in the content of the marker compounds of each extract observed during the time of investigation.

• The Korea Journal of Herbology
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• v.24 no.4
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• pp.189-195
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• 2009

Objectives : In this study, the effect of Dipsaci Radix(DR, Dipsacus asperoides C.Y. Cheng et T. M. Ai) water extract on LPS-induced inflammatory response in RAW264.7 cells were investigated. Methods : Dried roots of DR was extracted with water for 3 h(DR-W extract). RAW264.7 cells, a mouse macrophage line, were incubated with different concentrations of DR-W extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expression of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. Results : DR-W extract was significantly inhibited LPS-induced productions of NO and PGE2 in RAW264.7 cells. DR-W extract was not suppressed the expressions of iNOS mRNA and protein in LPS-stimulated RAW264.7 cells. Conclusions : This study suggests that DR-W extract can attenuate inflammatory response via inhibition of the NO and PGE2 production in activated macrophages.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.200.1-200.1
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• 2001

• The Korea Journal of Herbology
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• v.35 no.2
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• pp.15-29
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• 2020

Objectives : This study aimed to review the change in the origin of Sokdan(續斷) by diachronically analyzing literature data from Korea and China. Methods : Literature records describing the origin(synonyms, location of production, description) of Sokdan were collected, records were divided into periods. The main contents were included in the results, and original texts were edited and summarized in the table. Results : Sokdan, whose leaves resemble Jeoma(苧麻)(SJ), was first recorded in 《Xinxiubencao(新修本草)》, and described in detail in 《Bencaotujing(本草圖經)》 during the Tang and Song dynasties in China. In modern times, SJ was assumed to be genus Phlomoides or Lamium; however, records of the plant have decreased. Finally, SJ was not included in the 《Pharmacopoeia of the People's Republic of China》 as Sokdan. However, 《Diannanbencao(滇南本草)》, 《Diannanbencaotushuo(滇南本草圖說)》, 《Zhiwumingshitukao(植物名實圖考)》 described Sokdan of dian(滇). It was assumed genus Dipsacus. From the 1950s onwards, Sokdan is described in the literature as a member of the genus Dipsacus. In korea, SJ was recorded in 《Hyangyakjipseongbang(鄕藥集成方)》 and 《Dongeuibogam(東醫寶鑑)》 during the Joseon dynasty. In modern times, the genera Phlomoides and Lamium were mostly recorded as the origin of Sokdan. Conclusions : Several species have been described as Sokdan over the years in China, but since the 1950s, the genus Dipsacus was noted as the origin of Sokdan. In Korea, SJ was used in the past, thus Sokdan was recorded as P. umbrosa in 《The Korean Herbal Pharmacopoeia(KHP)·1985》. However, 《KHP·1998》 referred to P. umbrosa as Hansokdan and D. asperoides as Sokdan.

• Journal of Nutrition and Health
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• v.55 no.6
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• pp.617-629
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• 2022

Purpose: Osteoporosis is characterized by structural deterioration of the bone tissue because of the loss of osteoblastic activity or the increase in osteoclastic activity, resulting in bone fragility and an increased risk of fractures. Hederagenin (Hed) is a pentacyclic triterpenoid saponin isolated from Dipsaci Radix, the dried root of Dipsacus asper Wall. Dipsaci Radix has been used in Korean herbal medicine to treat bone fractures. In this study, we attempted to demonstrate the potential anti-osteoporotic effect of Hed by examining its effect on osteoblast differentiation in MC3T3-E1 cells. Methods: Osteoblastic MC3T3-E1 cells were cultured in 0, 1, and 10 ㎍/mL Hed for 3 and 7 days. The activity of alkaline phosphatase (ALP), bone nodule formation and level of expression of bone-related genes and proteins were measured in MC3T3-E1 cells exposed to Hed. The western blot test was used to detect the activation of the bone morphogenetic protein-2 (BMP2)/ Suppressor of Mothers against Decapentaplegic (SMAD)1 pathway. Results: Hed significantly increased the proliferation of MC3T3-E1 cells. Intracellular ALP activity was significantly increased in the 1 ㎍/mL Hed-treated group. Hed significantly increased the concentration of calcified nodules. Furthermore, Hed significantly upregulated the expression of genes and proteins associated with osteoblast proliferation and differentiation, such as Runt-related transcription factor 2 (Runx2), ALP, osteopontin (OPN), and type I procollagen (ProCOL1). Induction of osteoblast differentiation by Hed was associated with increased BMP2. In addition, Hed induced osteoblast differentiation by increasing the activity of SMAD1/5/8. These results suggest that Hed has the potential to prevent osteoporosis by promoting osteoblastogenesis in osteoblastic MC3T3-E1 cells via the modulation of the BMP2/SMAD1 pathway. Conclusion: The results presented in this study indicate that Hed isolated from Dipsaci Radix has the potential to be developed as a healthcare food and functional material possessing anti-osteoporosis effects.