The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Dipsaci Radix on Muscle Fiber Atrophy and MyoD Expression in Gastrocnemius of MeAO Rats

속단(續斷)이 중풍모델 흰쥐 비목근의 근섬유위축 및 MyoD 발현에 미치는 영향

  • Han, Sang-Woo (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Ryu, Sa-Hyun (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Shim, Eun-Sheb (Department of Anatomy & Institute, Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Dong-Eun (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Park, Min-Hee (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Bum-Hoi (Department of Anatomy & Institute, Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Hyun (Hamsoa Pharmaceutical Co. R&D Center) ;
  • Jung, Hyuk-Sang (Department of Anatomy & Institute, Oriental Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Nak-Won (Department of Neuroscience and Brain Disease, Graduate School of East-West Medicine, Kyung Hee University) ;
  • Sohn, Young-Joo (Department of Gynecology, College of Oriental Medicine, Sangji University)
  • 한상우 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 류사현 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 심은섭 (경희대학교 한의과대학 해부학교실, 한의학연구소) ;
  • 이동은 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 박민희 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 김범회 (경희대학교 한의과대학 해부학교실, 한의학연구소) ;
  • 최현 ((주)함소아제약 연구개발센터) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실, 한의학연구소) ;
  • 손낙원 (경희대학교 동서의학대학원 신경과학 및 뇌질환전공) ;
  • 손영주 (상지대학교 한의과대학 부인과학교실)
  • Published : 2008.06.30
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Abstract

Objectives : The present study has been undertaken to investigate the effects of Dipsaci Radix on Muscle Fiber Atrophy and MyoD Expression in Gastrocnemius of MCAO Rats Methods : In order to investigate effects of Dipsaci radix on the skeletal muscle atrophy following stroke, cerebral infarct was induced by the middle cerebral artery occlusion (MCAO) in the rats. Water extract of Dipsaci radix (184.4 mg/100 g) was treated for 4 weeks, once a day orally, after the MCAO. Effects were evaluated with muscle fiber type composition and cross-sectioned area of muscle fibers in gastrocnemius of the unaffected & affected hind limbs. And MyoD protein expression in gastrocnemius was demonstrated with immunohistochemistry and western blotting. Results : Obtained results were as follows; 1. Infarct volume was not attenuated by Dipsaci radix treatment in the MCAO rats. 2. At the affected-side hind limb of the MCAO rats, the increase of type-I fibers and the decrease of type-II fibers were induced by Dipsaci radix treatment. 3. At the affected-side hind limb of the MCAO rats, decreases of cross-sectioned areas of type-I and type-II fibers were attenuated by Dipsaci radix treatment. 4. At the affected-side hind limb of the MCAO rats, MyoD positive cells were increased by Dipsaci radix treatment. 5. At the affected-side hind limb of the MCAO rats, MyoD expressions were increased by Dipsaci radix treatment. Conclusions : These results suggest that Dipsaci radix has a protective effect against muscle atrophy, through the inhibition of the muscle cell apoptosis, following the central nervous system demage.

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