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Development studies of microalgae-based closed recirculating bivalves adults conditioning system: I. Induction of the gametogenesis (이매패류 어미관리를 위한 미세조류 기반 폐쇄-순환여과시스템 개발 연구: I. 성 성숙 유도)

  • Kim, Chung Yoo;Hur, Young Baek;Han, Jong Chul;Park, Young chul
    • The Korean Journal of Malacology
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    • v.32 no.3
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    • pp.231-240
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    • 2016
  • Techniques were developed for holding and conditioning of Pacific oysters, Crassostrea gigas, in a closed recirculating system. Experimental adults were used 500 oysters ( x two system, total 1,000 oysters) which were collected in $20^{th}$ March 2016 from long-line aquaculture farm at the south coast of Korea. During conditioning periods concentrated live microalgae as Isochrysis sp. $15{\times}10^7cells/mL$, Tetraselmis sp. $2{\times}10^7cells/mL$ and Pheaodactylum sp. $18{\times}10^7cells/mL$ were added 5 L every day, respectively which micro algae were functioned as diets and biological filter. Over all experimental periods total water exchange rate was 21.3% (daily 0.5%). Over 42 days conditioning, female and male oysters were maturated 90.9% and 94.4%, respectively. Survival rate was 98.7%. Mean shell hight (8.3 mm), total wet weight (19.2 g), meat wet weight (5.0 g) and shell wet weight (13.6 g) were significantly increased (P < 0.05). Water quality parameters including the water temperature ($22.1{\pm}0.4^{\circ}C$), salinity ($24.9{\pm}04$), dissolved oxygen (5.1-7.9 mg/L) and pH ($7.93{\pm}0.15$) were kept stable. Concentration of dissolved inorganic nutrient as ammonia (1.96-0.35 mg/L), nitrite (0.03-0.16 mg/L), nitrate (1.34-0.47 mg/L), DIP (0.42-0.03 mg/L) and silicate (3.83-0.00 mg/L) were significantly decreased throughout experiment except nitrite which was increased (P < 0.05), but nitrogenous components stayed below toxic levels (ammonia 0.0-5.5 mg/L, nitrite 0.0-460.0 mg/L) which indicated that closed recirculation system with microalgae based bio-filter could supply sufficiently environment condition to holding and conditioning of oyster.

Mineralogy and Genesis of Hydrothermal Deposits in the Southeastern Part of Korean Peninsula: (4) Kimhae Napseok Deposit (우리나라 동남부 지역의 열수광상에 대한 광물학적 및 광상학적 연구: (4) 김해납석광상)

  • Kim, Soo Jin;Choo, Chang Oh;Cho, Hyen Goo
    • Journal of the Mineralogical Society of Korea
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    • v.6 no.2
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    • pp.122-144
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    • 1993
  • The Kimhae napseok clay deposit was studied to characterize its mineralogy and genesis. Geology of the deposit is composed of Tertiary volcanic rocks and granodiorite. Tertiary volcanic rocks consist of andesitic tuff with minor interstratified tuffaceous shale, and rhyodacitic tuff. The main ore body of 2.4 to 4 m in thickness developed parallel to the bedding of andesitic tuff bed. Its strike and dip are $N70^{\circ}E-N85^{\circ}E$ and $16^{\circ}NW-32^{\circ}NW$, respectively. Two alteration zones; the propylitic zone of albite-epidote-chlorite-quartz assemblage and advanced argillic zone of pyrophyllite-dickite-alunite-diaspore assemblage are developed. Correlation of $SiO_2$ to $Al_2O_3$ shows no relation in propylitic zone, while a negative linear relation in advanced argillic zone. Chemical variation shows that $SiO_2$, $Al_2O_3$, MgO, CaO, $Na_2O$ and $K_2O$ were leached out during hydrothermal alteration. Pyrophyllite, the most abundant mineral in advanced argillic zone, occurs as low temperature 2M polytype. It is closely associated with dickite, diaspore and alunite. The Hinckley index of dickite is 0.83 showing moderate crystallinity. Na content is increasing in the M site with the increasing content of cations in the R-site. the mole percent of Na replacing K in alunite ranges from 53.2 to 71.6. It is also found that pyrophyllite grows in the dissolution site of diaspore. Plagioclase was albitized. Lowering of pH caused mainly by sulfide and sulfate decomposition resulted in preferential leaching of Si. It is inferred that aluminum released from plagioclase in the volcanic rocks as well as from the tuffaceous shale intercalated in andesitic tuff were the main sources of aluminum required for the formation of clay deposit. pH in hydrothermal fluid decreased from propylitic zone to advanced argillic zone with increasing degree of alteration. Based on experimental data reported in the literature and mineral assemblages, the formation temperature of the deposit ranges 270 to $320^{\circ}C$.

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Structural Evolution of the Eastern Margin of Korea: Implications for the Opening of the East Sea (Japan Sea) (한국 동쪽 대륙주변부의 구조적 진화와 동해의 형성)

  • Kim Han-Joon;Jou Hyeong-Tae;Lee Gwang-Hoon;Yoo Hai-Soo;Park Gun-Tae
    • Economic and Environmental Geology
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    • v.39 no.3 s.178
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    • pp.235-253
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    • 2006
  • We interpreted marine seismic profiles in conjunction with swath bathymetric and magnetic data to investigate rifting to breakup processes at the Korean margin leading to the separation of the Japan Arc. The Korean margin is rimmed by fundamental elements of rift architecture comprizing a seaward succession of a rift basin and an uplifted rift flank passing into the slope, typical of a passive continental margin. In the northern part, rifting occurred in the Korea Plateau, a continental fragment extended and partially segmented from the Korean Peninsula, that provided a relatively broader zone of extension resulting in a number of rifts. Two distinguished rift basins (Onnuri and Bandal Basins) in the Korea Plateau we bounded by major synthetic and smaller antithetic faults, creating wide and symmetric profiles. The large-offset border fault zones of these basins have convex dip slopes and demonstrate a zig-zag arrangement along strike. In contrast, the southern margin is engraved along its length with a single narrow rift basin (Hupo Basin) that is an elongated asymmetric half-graben. Rifting at the Korean margin was primarily controlled by normal faulting resulting from extension in the west and southeast directions orthogonal to the inferred line of breakup along the base of the slope rather than strike-slip deformation. Although rifting involved no significant volcanism, the inception of sea floor spreading documents a pronounced volcanic phase which seems to reflect slab-induced asthenospheric upwelling as well as rift-induced convection particularly in the narrow southern margin. We suggest that structural and igneous evolution of the Korean margin can be explained by the processes occurring at the passive continental margin with magmatism intensified by asthenospheric upwelling in a back-arc setting.

Geological Characteristics of Extra Heavy Oil Reservoirs in Venezuela (베네주엘라 초중질유 저류층 지질 특성)

  • Kim, Dae-Suk;Kwon, Yi-Kyun;Chang, Chan-Dong
    • Economic and Environmental Geology
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    • v.44 no.1
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    • pp.83-94
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    • 2011
  • Extra heavy oil reservoirs are distributed over the world but most of them is deposited in the northern part of the Orinoco River in Venezuela, in the area of 5,500 $km^2$, This region, which has been commonly called "the Orinoco Oil Belt", contains estimated 1.3 trillion barrels of original oil-in-place and 250 billion barrels of established reserves. The Venezuela extra heavy oil has an API gravity of less than 10 degree and in situ viscosity of 5,000 cP at reservoir condition. Although the presence of extra heavy oil in the Orinoco Oil Belt has been initially reported in the 1930's, the commercial development using in situ cold production started in the 1990's. The Orinoco heavy oil deposits are clustered into 4 development areas, Boyaco, Junin, Ayachoco, and Carabobo respectively, and they are subdivided into totally 31 production blocks. Nowadays, PDVSA (Petr$\'{o}$leos de Venzuela, S.A.) makes a development of each production block with the international oil companies from more than 20 countries forming a international joint-venture company. The Eastern Venezuela Basin, the Orinoco Oil Belt is included in, is one of the major oil-bearing sedimentary basins in Venezuela and is first formed as a passive margin basin by the Jurassic tectonic plate motion. The major source rock of heavy oil is the late Cretaceous calcareous shale in the central Eastern Venezuela Basin. Hydrocarbon materials migrated an average of 150 km up dip to the southern margin of the basin. During the migration, lighter fractions in the hydrocarbon were removed by biodegradation and the oil changed into heavy and/or extra heavy oil. Miocene Oficina Formation, the main extra heavy oil reservoir, is the unconsolidated sand and shale alternation formed in fluvial-estuarine environment and also has irregularly a large number of the Cenozoic faults induced by basin subsidence and tectonics. Because Oficina Formation has not only complex lithology distribution but also irregular geology structure, geological evolution and characteristics of the reservoirs have to be determined for economical production well design and effective oil recovery. This study introduces geological formation and evolution of the Venezuela extra heavy oil reservoirs and suggest their significant geological characteristics which are (1) thickness and geometry of reservoir pay sands, (2) continuity and thickness of mud beds, (3) geometry of faults, (4) depth and geothermal character of reservoir, (5) in-situ stress field of reservoir, and (6) chemical composition of extra heavy oil. Newly developed exploration techniques, such as 3-D seismic survey and LWD (logging while drilling), can be expected as powerful methods to recognize the geological reservoir characteristics in the Orinoco Oil Belt.

Effect of RGD peptide coating of implant titanium surface on human mesenchymal stem cell response (양극산화 티타늄 표면에 서로 다른 RGD 펩타이드 코팅 방법이 인간간엽줄기세포 반응에 미치는 영향)

  • Kim, Min-Su;Jeong, Chang-Mo;Jeon, Young-Chan;Ryu, Jae-Jun;Huh, Jung-Bo;Yun, Mi-Jung
    • The Journal of Korean Academy of Prosthodontics
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    • v.49 no.3
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    • pp.245-253
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    • 2011
  • Purpose: The aim of this in vitro study was to estimate surface characteristic after peptide coating and investigate biological response of human mesenchymal stem cell to anodized titanium discs coated with RGD peptide by physical adhesion and chemical fixation. Materials and methods: Fluorescence isothiocyanate (FITC) modified RGD-peptide was coated on the anodized titanium discs (diameter 12 mm, height 3 mm) using two methods. One was physical adhesion method and the other was chemical fixation method. Physical adhesion was performed by dip and dry procedure, chemical fixation was performed by covalent bond via silanization. In this study, human mesenchymal stem cell was used for experiments. The experiments consisted of surface characteristic evaluation after peptide coating, analysis about cell adhesion, proliferation, differentiation, and mineralization. Obtained data are statistically treated using Kruskal-Wallis test and Bonferroni test was performed as post hoc test (P=.05). Results: The evaluation of FE-SEM images revealed no diffenrence at micro-surfaces between each groups. Total coating dose was higher at physical adhesion experimental group than at chemical fixation experimental group. In cell adhesion and proliferation, RGD peptide coating did not show a statistical significance compared with control group (P>.05). In cell differentiation and mineralization, physical adhesion method displayed significantly increased levels compared with control group and chemical fixation method (P<.05). Conclusion: RGD peptide coating seems to enhance osseointegration by effects on the response of human mesenchymal stem cell. Especially physical adhesion method showed more effective than chemical fixation method on response of human mesenchymal stem cell.

Marine Environments and Ecological Characteristics of Phytoplankton in Southern Coastal Waters During June to October in 2004-2006 (2004-2006년 6-10월 동안의 남해중부연안 해역특성 및 식물플랑크톤의 군집생태)

  • Cho, Eun-Seob;Lee, Sang-Yong;Kim, Sang-Soo;Choi, Yoon-Seok
    • Journal of Environmental Science International
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    • v.16 no.8
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    • pp.941-957
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    • 2007
  • This study monitored marine environments and ecological characteristics of phytoplankton in southern coastal waters during June to October in 2004-2006 and provided an information to how Cochlodinium blooms have occurred. A total of sampling sites was 16 (Dukyang bay, Goheung, Yeoja bay, Gamak bay, Gwangyang bay, Yeosu, and Namhae). Temperature ranged from $19^{\circ}C\;to\;29^{\circ}C$, which all of sampling in Yeoja bay was the highest temperature of $27^{\circ}C\;and\;29^{\circ}C$ during summer. On June, July, September, and October did not show a remarkable difference regardless of sampling sites. Yeoja and Gwangyang bays had 25-27 in salinity that was lower approximately 5-6 compared with other sampling sites. Chlorophyll had considerable fluctuations depending on sampling sites on July and October, in particular, Gwangyang bay was the highest value of $15{\mu}gl^{-1}$ that had five times as much as. Unlikely to temperature, salinity, and chlorophyll, transparency ranged from 2 m to 5 m regardless of sampling sites. Gwangyang bay was the highest DIN (Dissolved Inorganic Nitrogen) of $0.53mgl^{-1}$ on July and August that had ten times as much as, whereas DIP (Dissolved Inorganic Phosphorus) did not show a significant difference between sampling sites. On July, the correlation of DIN and chlorophyll was a negative that should extremely require DIN to grow phytoplankton, but was a positive liner on August. Mean cell number of phytoplankton reached to encounter a peak of 500 cells $ml^{-1}$ in July and August, which diatoms were dominant species and attained an abundance of >60% regardless of months. In August, the occurrence of dinoflagellates ranged from 20% to 30%. Skeletonema costatum, one of dominant speicies, was the highest occurrence to throughout sampling sites during 2004 to 2006. On the basis of cluster analysis for phytoplankton, they were distributed in all of sampling sites. Consequently, significant fluctuations of marine environments were shown in summer and S. costatum was regarded as the representive phytoplankton in southern coastal waters. In particular, the outbreaks of Cochlodinium polykrikoides have occurred in Dukyang bay, Gamak bay, Goheung, Yeosu, and Namhae, but Yeoja and Gwangyang bays did not occur. The distinguish characteristics of occurring sampling sites and non-occurring in Cochlodinium blooms based on this study was DIN that was enough to persistently grow and maintain them even a litter dissolved in water. This suggests that Cochlodinium red tide seems to be occur in off waters.

The utility of ambulatory blood pressure monitoring in obese children (소아 비만아에서 ambulatory blood pressure monitoring의 유용성)

  • Kim, Myung Jin;Song, Jin Young
    • Clinical and Experimental Pediatrics
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    • v.51 no.6
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    • pp.604-609
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    • 2008
  • Purpose : Obesity in children and adolescence is highly correlated with adult obesity, which can provoke hypertension. Therefore, it is important to evaluate the blood pressure of obese children regularly. In this study, the results of ambulatory blood pressure monitoring (ABPM) in obese children and adolescents were evaluated. Methods : ABPM was conducted for selected patients who visited Handong University Sunlin Hospital from Feb. 1, 2006 to Dec. 1, 2007. The patients were classified into 3 groups: group 1 was normal, group 2 had normal casual blood pressure and a body mass index over the 95th percentile, and group 3 had high casual blood pressure over 120/80 mm Hg and a body mass index over the 95th percentile. Systolic and diastolic 24-hour blood pressure was measured, including both day and night. Results : There were 49 patients in the study. The results showed a significant difference for average systolic blood pressure between the three groups ($105.1{\pm}4.7$, $111.0{\pm}7.1$, $117.8{\pm}6.6mmHg$, P<0.001), but for average diastolic blood pressure only between groups 1 and 3 ($69.1{\pm}5.3$, $77.9{\pm}6.3mmHg$, P=0.001). In the daytime, only groups 1 and 3 showed a statistically significant difference for systolic and diastolic blood pressure. During the night the systolic pressure of group 3 was significantly higher than the other groups, but the diastolic pressure of group 3 was only higher than that group 1. No statistical difference was found in night dips among the groups. Conclusion : Ambulatory blood pressure monitoring in children and adolescents showed statistically higher blood pressure in obese patients with high casual blood pressure.

Geological Structures and Geochemical Uranium Anormal Zone Around the Shinbo Mine, Korea (신보광산 주변지역의 지질구조와 우라늄 지화학 이상대)

  • Kang, Ji-Hoon;Lee, Deok-Seon
    • Economic and Environmental Geology
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    • v.45 no.1
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    • pp.31-40
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    • 2012
  • This paper examined the characteristics of ductile and brittle structural elements with detailed mapping by lithofacies classification to clarify the relationship between the geological structure and the geochemical high-grade uranium anormal zone and to provide the basic information on the flow of groundwater in the eastern area of Shinbo mine, Jinan-gun, Jeollabuk-do, Korea. It indicates that this area is mainly composed of Precambrian quartzite, metapelite, metapsammite, which show a zonal distribution of mainly ENE-WSW trend, and age unknown pegmatite and Cretaceous porphyry which intrude them. But the Cretaceous Jinan Group which unconformably covers them, contrary to assumption, could not be observed. The main ductile deformation structures of Precambrian metasedimentary rocks were formed at least through three phases of deformation [ENE striking regional foliation (D1) -> ENE or EW striking crenulation foliation (D2) -> WNW or EW trending open, tight, kink folds (D3)]. The predominant orientation of S1 regional foliation strikes ENE and dips south, being similar to the zonal distribution of Precambrian metasedimentary rocks. Most predominant orientation of high-angled brittle fracture (dip angle ${\geq}45^{\circ}$) [ENE (frequency: 24.3%) > NS (23.9%) > (N)NW (18.8%) > WNW (16.9%) > NE (16.1%) fracture sets in descending frequency order], which is closely related to the flow of groundwater, strikes ENE and dips south. It also agrees with the zonal distribution of metasedimentary rocks and the predominant orientation of S1 regional foliation. The next one strikes NS and dips east or west. Considering the controlling factor of the geochemical uranium anormal zone in the Shinbo mine and its eastern areas from the above structural data. the uranium source rock in these areas might be pegmatite and the geochemical uranium anormal zone in the Sinbo mine area could be formed by an secondary enrichment through the flow of pegmatite aquifer's groundwater into the Sinbo mine area like the previous research's result.

Relationships between Texture and Physical Properties of Jurassic Unagsan and Cretaceous Sogrisan Granites (쥬라기 운악산 및 백악기 속리산 화강암류의 조직과 물성과의 관계)

  • Yun Hyun-Soo;Park Deok-Won;Hong Sei-Sun;Kim Ju-Yong;Yang Dong-Yoon;Chang Soobum
    • The Journal of Engineering Geology
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    • v.15 no.2 s.42
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    • pp.169-184
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    • 2005
  • Unagsan and Sogrisan granites are widely distributed in the northern Gyeonggi massif and middle Ogcheon belt, respectively, and they show different petrologic characteristics as follows. The former has compact textures and light grey colors, and the latter has spotted miarolitic textures and pink colors. Most of the samples selected for tests are fresh and coarse-grained. And bored core samples were prepared so that they are vertical to the rift plane. The results of modal analysis show that Unagsan granite has significantly higher quartz and plagioclase contents (Qz+Pl) than Sogrisan granite. In contrast, alkali feldspar content (Af) of Sogrisan granite is much higher than that of Unagsan granite. Therefore, it is believed that the light grey colors of Unagsan granite are due to relatively high Qz+Pl, and the pink colors of Sogrisan granite are caused by higher Af. Fractures in Sogrisan granite have strongly perpendicular strike patterns and more dip values close to vertical compared with the fractures in Unagsan granite. Results of the fracture pattern analysis suggest that the Sogrisan granite has better potential to produce dimension stones than the Unagsan granite. However, miarolitic textures often found in the Sogrisan granite may be one of the factors reducing the granite quality. The Unagsan and Sogrisan granites have similar specific gravity values of 2.60 and 2.57, respectively. Absorption ratios and porosity values of Sogrisan granite are higher than those of Unagsan granite, and they shows linearly positive correlations. Compressive and tensile strengths of the Unagsan granite are generally higher than those of Sogrisan granite. These differences and variation trends found in physical properties of Unagsan and Sogrisan granite can be explained by the differences in the textures of Unagsan and Sogrisan granites, namely compact and miarolitic textures respectively. For Unagsan granite, compressive and tensile strengths are negatively correlated with porosity but for Sogrisan granite no specific correlations are found. This is probably due to the irregular dispersion patterns of miarolitic textures formed during the later stages of magmatic processes. Contrary to the trends found in absorption ratios, both granites have similar values of abrasive hardness, which can be explained by higher Qz+Af of the Sogrisan granite than those of the Unagsan granite and that quartz and alkali feldspar have relatively larger hardness values. For Sogrisan granite, compressive strength shows slightly positive correlations with Qz+Af+Pl and negative correlations with biotite and accessory mineral contents (Bt+Ac).

Time-lapse crosswell seismic tomography for monitoring injected $CO_2$ in an onshore aquifer, Nagaoka, Japan (일본 Nagaoka의 육상 대수층에 주입된 $CO_2$의 관찰을 위한 시간차 시추공간 탄성파 토모그래피)

  • Saito, Hideki;Nobuoka, Dai;Azuma, Hiroyuki;Xue, Ziqiu;Tanase, Daiji
    • Geophysics and Geophysical Exploration
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    • v.9 no.1
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    • pp.30-36
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    • 2006
  • Japan's first pilot-scale $CO_2$ sequestration experiment has been conducted in Nagaoka, where 10400 t of $CO_2$ have been injected in an onshore aquifer at a depth of about 1100 m. Among various measurements conducted at the site for monitoring the injected $CO_2$, we conducted time-lapse crosswell seismic tomography between two observation wells to determine the distribution of $CO_2$ in the aquifer by the change of P-wave velocities. This paper reports the results of the crosswell seismic tomography conducted at the site. The crosswell seismic tomography measurements were carried out three times; once before the injection as a baseline survey, and twice during the injection as monitoring surveys. The velocity tomograms resulting from the monitoring surveys were compared to the baseline survey tomogram, and velocity difference tomograms were generated. The velocity difference tomograms showed that velocity had decreased in a part of the aquifer around the injection well, where the injected $CO_2$ was supposed to be distributed. We also found that the area in which velocity had decreased was expanding in the formation up-dip direction, as increasing amounts of $CO_2$ were injected. The maximum velocity reductions observed were 3.0% after 3200 t of $CO_2$ had been injected, and 3.5% after injection of 6200 t of $CO_2$. Although seismic tomography could map the area of velocity decrease due to $CO_2$ injection, we observed some contradictions with the results of time-lapse sonic logging, and with the geological condition of the cap rock. To investigate these contradictions, we conducted numerical experiments simulating the test site. As a result, we found that part of the velocity distribution displayed in the tomograms was affected by artefacts or ghosts caused by the source-receiver geometry for the crosswell tomography in this particular site. The maximum velocity decrease obtained by tomography (3.5%) was much smaller than that observed by sonic logging (more than 20%). The numerical experiment results showed that only 5.5% velocity reduction might be observed, although the model was given a 20% velocity reduction zone. Judging from this result, the actual velocity reduction can be more than 3.5%, the value we obtained from the field data reconstruction. Further studies are needed to obtain more accurate velocity values that are comparable to those obtained by sonic logging.