• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.226-227
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• 2011

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.156-156
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.157-157
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.146-146
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• 2003

• Proceedings of the Korean Society of Crop Science Conference
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• 2002.10a
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• pp.58-58
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• 2002

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.127.2-127
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• 2003

YMV-K strains were purified from D. oppostita Thunb. tv. Dung-Gun-Ma showing mosaic symptom on their leaves. YMV-K strains were filamentous particles of 780nm in length and induced cytoplasmic disorder such as inclusion body formation. Nucleotide and amino acid sequences of 5'-UTR, Pl and CP of YMV-K strains shared 80.8, 64.7 and 98.3％ identity respectively to JYMV J1 in the mean value. Purification of YMV-K strains according to JYMV purification method(S. Fuji) was conducted to product antiserum. With antiserum against YMV-K strains, the Various diagnosis methods such as IC-RT-PCR, DIBA, RIPA and indirect-ELISA were used to detect YMV-K strains in Chinese yam plant.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.99-103
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• 2007

Tuber yield and content of general component and diosgenin which is a main bioactive property were investigated in order to determine the growth characteristics of round typed yam (Dungeon-ma, Dioscorea opposita L.) and the potential of artificial culture at Suwon, Korea. Sprouting rates of segmented small tubers such as 3, 5, 7, 9, and 12g were respectively 60.3, 80.5, 85.3, 98.6 and 99.3% when they were investigated 30 days after planting. Segmented small tubers of 3, 5, 7, 9, and 12g enlarged respectively to 66, 87, 98, 120, and 140g in the green-house cultivation. Segmented small tubers of 3, 7, 12, 18, and 23g enlarged respectively to 35.2, 124.7, 142.7, 174.8, and 200,7g in the open-air field cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.68-72
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• 2003

The metabolism of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in the Chinese yam (Dioscorea opposita Thunb. var. Tsukune) was examined to determine the identification of endogenous gibberellins. [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ was metabolized to $\textrm{GA}_{53}$, $\textrm{GA}_{44}$, $\textrm{GA}_{19}$, $\textrm{GA}_{20}$, $\textrm{GA}_1$, $\textrm{GA}_8$, $\textrm{GA}_{15}$, $\textrm{GA}_{24}$, $\textrm{GA}_9$, $\textrm{GA}_{36}$ and $\textrm{GA}_4$. Radioactivity of GAs in non C-13 hydroxylation route was five-fold higher than that of early C-13 hydroxylation in analyzed GA-metabolites. Radioactivity of $\textrm{GA}_4$ was always four times higher than that of $\textrm{GA}_1$ at every feeding time. $\textrm{GA}_1$ radioactivity has always a lower level to below 200 DPM. The major pathway of endogenous GA metabolism in seedlings of the Chinese yam might be the non C-13 hydroxylation pathway.

• Journal of Plant Biotechnology
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• v.6 no.2
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• pp.97-102
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• 2004

This study was carried out to establish The regeneration of healthy seedlings from the nodal segment culture of Chinese yam (Dioscorea opposita cv. Danma), cultivated in Korea. Different explants such as leaves, petioles, roots and nodal pieces, excised from the in vitro grown seedlings of Chinese yam, were cultured on MS medium supplemented with various combinations of growth regulators. All the growth regulators used induced plantlet regeneration from the nodal segments at a high frequency, while there was no induction of shoot or callus from leaf, petiole or root tissues. The medium supplemented with 0.01mg/L NAA, 0.5mg/L BA, 0.5-1.0mg/L kinetin and without plant growth regulator was effective for shoot development of buds from the nodal segment culture. The concentration of BA and NAA was an important factor in the bud induction of buds from the nodal segments of Chinese yam. Nodal segments cultured on the medium containing 1.0mg/L NAA and 0.5-1.0mg/L BA gave the best response to bud formation. The addition of GA$_3$ to the culture medium suppressed shoot induction and growth, while it increased microtuber formation. The shoot growth and microtuber formation were also affected by medium strength and solidity. The MS basal medium containing 1 g/L gelrite was suitable for microtuber formation from the nodal segment of Chinese yam.

• Food Industry And Nutrition
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• v.17 no.2
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• pp.20-22
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• 2012

본 연구에서는 둥근마와 장마의 일반성분을 분석하여 둥근마의 우수한 성질을 밝히며, 에탄올 농도를 달리(0~95%, v/v)한 용매로 둥근마를 추출한 후 항산화활성을 측정함으로써 둥근마의 항산화성분을 최대한 함유할 수 있는 최적의 에탄올농도를 찾아, 이 추출물을 활용한 다양한 기능성 함유 음료의 제조방법을 개발하고자 하였다. 일반 성분 분석결과 둥근마의 경우 탄수화물, 열량, 단백질 및 철 함량이 장마와 비교하여 월등히 높은 값을 나타내었고, 식이섬유 및 칼륨 함량 또한 비교적 높았다. 47.5~95% (v/v) 에탄올 함유 용매로 추출할 경우 순수한 물로 추출한 경우보다 더 높은 항산화 활성을 나타내었다. 전자공여능의 경우 추출용매의 에탄올 농도가 높을수록 높은 값을 나타내었으며, 반면에 총 폴리페놀성 화합물함량의 경우 71.25% 농도의 에탄올로 추출한 추출액에서 가장 높은 값을 나타내었다. 따라서 에탄올농도 약 70~95% 정도의 용매로 추출한 둥근마 추출액 함유 음료를 제조함으로써 항산화 성분을 다량함유 한 음료를 제공할 수 있을 것이며, 또한 둥근마 추출액에 첨가물 및 기타 기능성 성분을 혼합하여 음료를 제조함으로써 기호성이 증가되고 기능성이 향상된 다양한 음료를 제공할 수 있을 것으로 사료된다.