Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization and diagnosis of YMV-K strains infecting Chinese yam(Dioscorea opposita Thunb. cv. Dung-Gun-Ma) in Korea. (oral)

  • D. K. Kang (Experiment Station of Bioresources, Gyeungbuk Provincial ATA) ;
  • H. Y. Shin (Dept. Biology, Yeungnam University) ;
  • J. H. Sung (Dept. Biology, Yeungnam University) ;
  • Park, J. H. (Dept. Biology, Yeungnam University) ;
  • M. U. Chang (Dept. Biology, Yeungnam University)
  • Published : 2003.10.01
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Abstract

YMV-K strains were purified from D. oppostita Thunb. tv. Dung-Gun-Ma showing mosaic symptom on their leaves. YMV-K strains were filamentous particles of 780nm in length and induced cytoplasmic disorder such as inclusion body formation. Nucleotide and amino acid sequences of 5'-UTR, Pl and CP of YMV-K strains shared 80.8, 64.7 and 98.3％ identity respectively to JYMV J1 in the mean value. Purification of YMV-K strains according to JYMV purification method(S. Fuji) was conducted to product antiserum. With antiserum against YMV-K strains, the Various diagnosis methods such as IC-RT-PCR, DIBA, RIPA and indirect-ELISA were used to detect YMV-K strains in Chinese yam plant.

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