• 제목/요약/키워드: Differential interference contrast

검색결과 24건 처리시간 0.021초

편광 격자 기반 정량적 위상 이미징을 위한 미분 간섭 현미경 모듈 개발 (Differential Interference Contrast Microscopic Module Using a Polarization Grating for Quantitative Phase Imaging)

  • 조진희;주기남
    • 한국광학회지
    • /
    • 제34권6호
    • /
    • pp.261-268
    • /
    • 2023
  • 본 연구에서는 간단한 구조의 층밀림 간섭계 기반의 정량적 위상 이미징을 위한 미분 간섭 현미경 모듈을 제안한다. 제안하는 모듈은 복굴절성과 편광 광 분할기의 특성을 갖는 기하 위상 소자 중의 하나인 편광 격자를 이용하여 층밀림 간섭계 구조로 구성되고, 편광 카메라를 통해 획득된 4개의 편광 영상으로 정량적인 위상차 영상뿐만 아니라 일반적인 명시야 현미경 영상을 동시에 추출할 수 있다. 또한, 제안하는 모듈의 실시간 영상 획득 가능성, 안정성 및 소형화로 인해 기존의 현미경에 편리하게 적용할 수 있다. 제안하는 시스템을 검증하기 위해 다양한 시편에 대한 명시야 영상 및 위상차 영상을 획득하였고, 또한 이들 영상들을 합성하여 보다 가독성이 높은 시편 영상을 획득하였다.

Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse

  • Yong Soo Park;Yeonji Kim;Sung Won Kim; In-Beom Kim
    • Applied Microscopy
    • /
    • 제50권
    • /
    • pp.11.1-11.3
    • /
    • 2020
  • The human turbinate-derived mesenchymal stem cells (hTMSCs), which were DiI-labeled and transplanted into the subretinal space in degenerating mouse retina, were observed in retinal vertical sections processed for rhodopsin (a marker for rod photoreceptor) by confocal microscope with differential interference contrast (DIC) filters. The images clearly demonstrated that DiI-labeled hTMSCs have rhodopsin-immunoreactive appendages, indicating differentiation of transplanted hTMSC into rod photoreceptor. Conclusively, the finding suggests therapeutic potential of hTMSCs in retinal degeneration.

Redescription of Free-Living Marine Nematode, Dracograllus filipjevi Allen and Noffsinger, 1978 (Nematoda: Draconematidae) from Korea

  • Rho, Hyun-Soo;Kim, Dong-Sung;Kim, Won
    • Ocean Science Journal
    • /
    • 제41권3호
    • /
    • pp.163-173
    • /
    • 2006
  • A draconematid nematode species, Dracograllus filipjevi Allen and Noffsinger, 1978, is described from the washings of shallow littoral coralline algae at Guryongpo in the eastern coast of Korea. This species is recognized by the following characteristics: larger body size, number of posterior sublateral adhesion tubes (9 in male and 12-13 in female) and subventral adhesion tubes (8-9 in male and 9-10 in female), the absence of cephalic acanthiform setae on rostrum, the presence of longitudinally areolated body cuticle with dot-like punctations, the presence of some cuticular collar in swollen pharyngeal region, the shape of amphidial fovea (large, elongate, and loop-shaped in both sexes), the absence of preanal corniform setae, and the absence of lateral differentiation on narrow body region. Morphological features of the species using differential interference contrast photomicrographs are described in detail in the present work. The is the first report of D. filipjevi in Korea.

A new free-living marine nematode species of the genus Phanoderma Bastian, 1865(Enoplida: Phanodermatidae) from the East Sea, Korea

  • Lee, Hyo Jin;Rho, Hyun Soo
    • 환경생물
    • /
    • 제37권3호
    • /
    • pp.396-405
    • /
    • 2019
  • A new species of free-living marine nematode is described from intertidal sediments of the East Sea, Korea. Phanoderma koreense sp. nov. is characterized by the presence of well-developed pharyngeal and cephalic capsule, six inner labial sensilla present as minute papillae around with circular groove, long and slender spicules with 4-5 serrated distal end, located at the base of the precloacal supplement, a series of eight to nine stout and short setae on the ventral cloacal region and conico-cylindrical tail with two pairs of blunt setae. In this study, we provide taxonomic descriptions and illustrations of a new species by differential interference contrast microscope and a pictorial key to the valid species of Phanoderma Bastian, 1865. This is the first record of the genus Phanoderma in the East Sea, Korea.

Two unrecorded marine nematode species of Paracanthonchus (Nematoda: Cyatholaimidae) from the East Sea of Korea

  • Lee, Hyo Jin;Jung, Jongwoo;Rho, Hyun Soo
    • Journal of Species Research
    • /
    • 제5권3호
    • /
    • pp.503-513
    • /
    • 2016
  • Two unrecorded marine nematode species, belonging to genus Paracanthonchus Micoletzky, 1924, are described on the basis of the specimens collected from rocky intertidal seagrass bed on the eastern coast of Korea. Paracanthonchus macrodon (Ditlevsen, 1918) is characterized by presence of cuticular differentiation, strongly dilated gubernaculum with numerous cusps, three post-cloacal setae, and arrangement of six (4+2) precloacal supplement. Paracanthonchus kamui Kito, 1981 is distinguished from other species of the genus by having the 4.25 turns amphideal fovea, six tubular supplement, rib-shaped gubernaculums with proximally hook-shaped and distally broaden. In this study, we provide taxonomic description and illustration of two unrecorded species of the genus Paracanthonchus by differential interference contrast microscopy. This is the first report on the species of the genus Paracanthonchus from Korea.

플랑크톤 모니터링 네트워크를 위한 Triadinium속의 동정 (Identification of Genus Triadinium for Plankton Monitoring Network)

  • 여환구
    • 한국산학기술학회:학술대회논문집
    • /
    • 한국산학기술학회 2009년도 춘계학술발표논문집
    • /
    • pp.842-844
    • /
    • 2009
  • The major group which contains all the organisms that are commonly termed Dinoflagellates is called the division Pyrrophyta or Dinophyta in botanical systems and the order Dinoflagellida or Dinoflagellata in zoological systems. Most dinoflagellates are biflagellated solitary planktonic cells. A taxonomic survey of the dinoflagellate family, Triadiniaceae Dodge was conducted for plankton monitoring network in the coastal waters of Korea. Identification of species in water samples was usually done by using differential interference contrast (DIC), which revealed especially well lighted thecal structures. For the apparent three-dimensional image, scanning electron microscope (SEM) was used. Two species were identified and described, which were Triadinium orientale(Lindemann)Dodge and Triadinium sphaericum(Murray and Whitting)Dodge.

  • PDF

First Record of Desmoscolex Nematoda (Desmoscolecida: Desmoscolecidae) from Korea

  • Lim, Hyung-Wook;Chang, Cheon-Young
    • Animal cells and systems
    • /
    • 제10권4호
    • /
    • pp.219-225
    • /
    • 2006
  • As a result of the faunistic survey of the free-living marine nematodes from coastal algal beds and sublittoral sands in South Korea, we report a meiobenthic species, Desmoscolex cosmopolites Timm, 1970, for the first time from the western Pacific as well as Korean waters. Among 17-ring Desmoscolex species, D. cosmopolites is represented by the following combination of characters: absence of subventral setae on 10th and 14th main rings and the presence of subdorsal setae with large angular lance-shaped tip. A detailed morphological redescription is provided using a scanning electron microscope and a differential interference contrast microscope. Character comparison between D. cosmopolites and its allied congeneric species is also analysed.

A New Species of the Genus Agauopsis (Acari: Halacaridae) from Jeju Island, Korea

  • Shin, Jong Hak;Chang, Cheon Young
    • Animal Systematics, Evolution and Diversity
    • /
    • 제35권2호
    • /
    • pp.49-56
    • /
    • 2019
  • A new marine halacarid species of the genus Agauopsis Viets, 1927 (Acari: Halacaridae) is recorded from Jeju Island, Korea. Agauopsis tetrasetosa n. sp. belongs to the microrhyncha group in sharing one ventral and three ventromedial spines on tibia of leg I, of which two ventromedial ones are adjacent to each other, with its 20 congeneric members. Agauopsis tetrasetosa n. sp. is most similar to A. miliaris Bartsch, 2005 and A. similis Bartsch, 1979 in sharing the similar chaetotaxy of legs I-IV, positions of the second and fourth dorsal setae, and non 'H'- or 'M'-shaped costae on anterior dorsal plate. However, the new species is distinguished from them by four pairs of perigenital setae in female, the relatively anterior location of gland pores on posterior dorsal plate, and three ventral spines on the tibia of leg II. We describe the new species herein, with detailed illustrations under a differential interference contrast microscope. We also make some brief comments on the affinities between the congeneric species of the microrhyncha group and the new species, based on a tabular key to species of the group.

A New Free-Living Marine Nematode, Chaetonema longicorpus sp. nov. (Enoplida: Anoplostomatidae) from a Subtidal Zone of the East Sea, Korea

  • Lee, Hyo Jin;Lee, Heegab;Rho, Hyun Soo
    • Animal Systematics, Evolution and Diversity
    • /
    • 제38권4호
    • /
    • pp.205-213
    • /
    • 2022
  • A new free-living marine nematode, Chaetonema longicorpus sp. nov., was discovered in a subtidal benthic habitat around the Uljin nuclear power plant in the East Sea. Chaetonema longicorpus sp. nov. differs from other Chaetonema species in its very long body length, relatively long cephalic setae, long and narrow buccal cavity, bottle-shaped amphideal fovea, short spicules, only one pre-cloacal seta instead of a pre-cloacal supplement, and conspicuous ventral swelling at the middle of the tail. Herein, we provide a taxonomic description and illustrations of this new species using differential interference contrast microscopy. Furthermore, an illustrated pictorial key to all valid species, including the new species and comparative tables on the biogeographical and morphological characteristics of the genus Chaetonema, are provided.

Admirandus multicavus Belogurov and Belogurova, 1979 (Nematoda, Enoplida, Oncholaimidae), a new record of free-living marine nematode discovered from the intertidal zone of Korea

  • Hyun Soo Rho;Hyo Jin Lee;Heegab Lee
    • 환경생물
    • /
    • 제39권4호
    • /
    • pp.463-470
    • /
    • 2021
  • Admirandus multicavus Belogurov and Belogurova, 1979, a new record of free-living marine nematode species belonging to family Oncholaimidae is reported based on the specimens collected from the intertidal sediments of Korea. Admirandus multicavus is characterized by the following unique characteristics: cephalic setae (1-2 ㎛ long), spicules (71-126 ㎛ long) and gubernaculum (15-31 ㎛ long), midventral preanal supplementary organ, three glandular duct openings of the Demanian system, and stoma length measuring twice the width. A comparative analysis of the biogeographical and ecological characteristics of Admirandus species is presented. DIC (Differential interference contrast) photomicrographs of the species are also provided herein. This report represents the first taxonomic analysis of the genus Admirandus Belogurov and Belogurova, 1979 discovered from the Korean seawaters.