• Molecules and Cells
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• 제47권3호
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• pp.100033.1-100033.13
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• 2024

Considering the recent increase in the number of colorectal cancer (CRC) cases in South Korea, we aimed to clarify the molecular characteristics of CRC unique to the Korean population. To gain insights into the complexities of CRC and promote the exchange of critical data, RNA-sequencing analysis was performed to reveal the molecular mechanisms that drive the development and progression of CRC; this analysis is critical for developing effective treatment strategies. We performed RNA-sequencing analysis of CRC and adjacent normal tissue samples from 214 Korean participants (comprising a total of 381 including 169 normal and 212 tumor samples) to investigate differential gene expression between the groups. We identified 19,575 genes expressed in CRC and normal tissues, with 3,830 differentially expressed genes (DEGs) between the groups. Functional annotation analysis revealed that the upregulated DEGs were significantly enriched in pathways related to the cell cycle, DNA replication, and IL-17, whereas the downregulated DEGs were enriched in metabolic pathways. We also analyzed the relationship between clinical information and subtypes using the Consensus Molecular Subtype (CMS) classification. Furthermore, we compared groups clustered within our dataset to CMS groups and performed additional analysis of the methylation data between DEGs and CMS groups to provide comprehensive biological insights from various perspectives. Our study provides valuable insights into the molecular mechanisms underlying CRC in Korean patients and serves as a platform for identifying potential target genes for this disease. The raw data and processed results have been deposited in a public repository for further analysis and exploration.

• 대한토목학회논문집
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• 제4권1호
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• pp.93-101
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• 1984

본(本) 논문(論文)에서는 일정변단면장주(一定變斷面長柱)의 탄성임좌굴하중(彈性臨挫屈荷重)을 구하는 각산식(咯算式)을 제시(提示)하였다. 특히, 고가도로설계시(高架道路設計時) 자주 나타나는 일단고정(一端固定) 타단자유(他端自由)인 중실원형(中實圓形) 및 구형단면(矩形斷面)에 한(限)하였다. 처짐곡선 미분방정식(微分方程式)의 정밀해(精密解)는 Bessel 함수(凾數)로 나타나는데, Bisection method를 이용하여 Computer로 수치해석(數値解析)하였다. 또 자중(自重)고려시 F.E.M에 의한 해석(解析)은 고유치(固有値) 문제(問題)가 되므로 Jacobi method를 이용하여 수치해석(數値解析)하였고, 균일단면장주(均一斷面長柱)의 정밀해(精密解)와 비교(比較)하여 F.E.M에 의한 근사해(近似解)의 신뢰도(信賴度)를 높였다. 그 결과(結果) 일정변단면장주(一定變斷面長柱)의 임계좌굴하중(臨界挫屈荷重)은 형상계수(形狀係數)와 단면변화율(斷面變化率)에 대해 균일단면장주(均一斷面長柱)의 비례식(比例式)으로 나타낼 수 있었다.

• Molecules and Cells
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• 제25권1호
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• pp.78-85
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• 2008

In a search for new molecular pathways associated with asthma, we performed an mRNA differential display analysis using total RNA extracted from the tracheal tissues of ovalbumin (OVA)-challenged mice and sham controls. cDNAs corresponding to mRNAs for which expression levels were altered by OVA-challenge were isolate and sequenced. Twenty-eight genes differentially expressed in sham and OVA challenged mice were identified. A GenBank BLAST homology search revealed that they were related to cytoskeleton remodeling, transcription, protein synthesis and modification, energy production, and cell growth and differentiation. Two were selected for further characterization. Up-regulation of both the perinatal skeletal myosin heavy chain (skMHC) and fast skeletal muscle myosin light chain (skMLC) genes was confirmed by RT-PCR of trachea tissue from OVA challenged mice. Overexpression of skMLC protein was observed in the smooth muscle layers of OVA-challenged mice by immunohistochemistry, and the surface areas stained with skMLC antibody increased in the OVA-challenged mice. The overexpression of skMLC in murine asthma may be associated with the changes of bronchial smooth muscle.

• 한국의류학회지
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• 제18권5호
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• pp.646-660
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• 1994

The purpose of this study was to investigate the difference of the visual evaluation about Clothing form and the surface image of detail. This study consists of pre-experiment for selecting the method of expression among detail which shows difference of the image and main experiment for identifying the clothing image as clothing form and the suface image of detial. Main experiment is made of factorial design for three variables-clothing form (H-line, A-line, V-line, X-line), detail (frill, tape), direction (width, length). Questionaire consists of 24 semantic differential scale expressing clothing form and detail. The subjects were 100 female students majoring in clothing and textile.7he data were analyzed by Frequencey, Factor analysis, Anova, scheffe's test and MCA method. The major findings were; 1) The image of clothing form and the surface image of detail were composed of 5 factors; attractiveness, prettiness, attention, modern, young. 2) For the visual evaluation of clothing form as the surface image of detail, there were significant differences in prettiness and attention factors. For the pretty and attentive image, we should express by the image of frill. 3) For the visual evaluation of the image of detail as clothing forms variation, there were significant difference in prettiness by A-line and X-line. 4) For the effect of clothing form and the surface image of detail, main effect was significant in attractiveness, prettiness, attention, modern factor. For the pretty image of clothing, it will be expressed by the image of frill and A-line, X-line. For the attentive image of clothing, it will be expressed by the image of frill and V-line. For the modern image of clothing, it will be expressed by the image of tape and V-line.

• 한국발생생물학회지:발생과생식
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• 제9권1호
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• pp.33-41
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• 2005

배반포 단계의 난자에서 차이 나게 발현하는 유전자의 발굴을 통해 초기 동물 발생과 분화에 관한 기전을 알 수 있다. 본 연구에서는 새로운 차별발현 역전사효소중합법, 이름하여 에닐링 콘트롤 프라이머(ACP) 방법에 의해 생쥐 배반포에서 차이 나게 발현하는 유전자를 줄기세포와 비교하여 발굴하였다. 총 100개의 ACP를 사용하여 26개의 유전자 단편을 확인하였고, BLAST 탐색에 의해 유전자 정보 은행(GeneBank/EMBL)에 저장된 유전자와 동일하다는 것을 알았다. 이들 유전자 중에 15개의 유전자를 선별하여 역전사효소중합법에 의해 조사한 결과, 배반포에서 차이 나게 발현함을 재확인하였다. 이러한 결과는 ACP 방법이 특정 발달 단계에 있는 소량의 배아 샘플로부터 전사되는 유전자를 확인하는데 실용적으로 사용될 수 있다는 것을 보여주고 있다.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4397-4402
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• 2011

The NF-${\kappa}B$ system of transcription factors plays a crucial role in inflammatory diseases, making it an important drug target. We combined quantitative structure activity relationships for predicting the activity of new compounds and quantitative dynamic models for the NF-${\kappa}B$ network with intracellular concentration models. GFA-MLR QSAR analysis was employed to determine the optimal QSAR equation. To validate the predictability of the $IKK{\beta}$ QSAR model for an external set of inhibitors, a set of ordinary differential equations and mass action kinetics were used for modeling the NF-${\kappa}B$ dynamic system. The reaction parameters were obtained from previously reported research. In the IKKb QSAR model, good cross-validated $q^2$ (0.782) and conventional $r^2$ (0.808) values demonstrated the correlation between the descriptors and each of their activities and reliably predicted the $IKK{\beta}$ activities. Using a developed simulation model of the NF-${\kappa}B$ signaling pathway, we demonstrated differences in $I{\kappa}B$ mRNA expression between normal and different inhibitory states. When the inhibition efficiency increased, inhibitor 1 (PS-1145) led to long-term oscillations. The combined computational modeling and NF-${\kappa}B$ dynamic simulations can be used to understand the inhibition mechanisms and thereby result in the design of mechanism-based inhibitors.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.120-120
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• 2017

Since the composition of proteins from the Korean cultivars of Proso millet is unknown; thereby, the present study was conducted to obtain a reference map of millet seed proteins and identify the functional characteristics of the identified proteins. Proteins extracted from the millet seeds of various cultivars, were investigated using proteomic techniques as 2D electrophoresis coupled with mass fingerprinting. The 1152 (differentially expressed) proteins were detected on 2-D gel. Among them, 26 reproducible protein spots were analyzed by MALDI-TOF-TOF mass spectrometry. Out of 26 proteins, 2 proteins were up-regulated towards all cultivars of millet, while 7 proteins were up-regulated and 13 proteins were down-regulated against only one cultivar. However, abundance in most identified protein species, associated with metabolism, transcription and transcription was significantly enhanced, while that of another protein species involved in polysaccharide metabolism, stress response and pathogenesis were severely reduced. Taken together, the results observed from the study suggest that the differential expression of proteins from the four cultivars of millet may be cultivar-specific. Taken together, a proteomic investigation of millet seeds from different cultivars, we sought to better understand the genetic variation of millet cultivars representing the future millet research, and the functional categorization of individual proteins on the basis of their molecular function.

• 대한수학교육학회지:수학교육학연구
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• 제27권1호
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• pp.1-22
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• 2017

본 연구에서는 고등학교 1학년 학생들을 대상으로 학생들이 함숫값의 변화를 인식하는 과정에서 어떠한 변화율 개념을 가지고 있는지 확인하고, 변화율 개념에 따라 어떻게 구간에서 함수의 변화를 인식하고 표현하는지에 대하여 6차시에 걸친 교수실험을 실시하였다. 그 결과 학생들이 함수의 변화를 분석하는데 변화율 개념을 이용되기는 하지만, 학생들의 변화율에 대한 인식과 표현이 다양하고 이에 따라 평균변화율에 대한 인식에 있어서도 차이가 나타나는 것으로 관찰되었다. 다만 이 차이를 질적인 수준차로 보아야할 것인지에 대하여는 추후 연구가 필요할 것으로 보인다. 본 연구는 변화율에 대한 학생의 인식을 세밀하게 조사한 연구로써 향후 변화율 관점에서의 미분학습에 대한 연구에 기초자료가 될 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1353-1362
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• 2016

Hanwoo, a Korean native cattle (Bos taurus coreana), has great economic value due to high meat quality. Also, the breed has genetic variations that are associated with production traits such as health, disease resistance, reproduction, growth as well as carcass quality. In this study, next generation sequencing technologies and the availability of an appropriate reference genome were applied to discover a large amount of single nucleotide polymorphisms (SNPs) in ten Hanwoo bulls. Analysis of whole-genome resequencing generated a total of 26.5 Gb data, of which 594,716,859 and 592,990,750 reads covered 98.73% and 93.79% of the bovine reference genomes of UMD 3.1 and Btau 4.6.1, respectively. In total, 2,473,884 and 2,402,997 putative SNPs were discovered, of which 1,095,922 (44.3%) and 982,674 (40.9%) novel SNPs were discovered against UMD3.1 and Btau 4.6.1, respectively. Among the SNPs, the 46,301 (UMD 3.1) and 28,613 SNPs (Btau 4.6.1) that were identified as Hanwoo-specific SNPs were included in the functional genes that may be involved in the mechanisms of milk production, tenderness, juiciness, marbling of Hanwoo beef and yellow hair. Most of the Hanwoo-specific SNPs were identified in the promoter region, suggesting that the SNPs influence differential expression of the regulated genes relative to the relevant traits. In particular, the non-synonymous (ns) SNPs found in CORIN, which is a negative regulator of Agouti, might be a causal variant to determine yellow hair of Hanwoo. Our results will provide abundant genetic sources of variation to characterize Hanwoo genetics and for subsequent breeding.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.895-902
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• 2012

The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.