• 식물조직배양학회지
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• 제22권3호
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• pp.169-173
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• 1995

근관 표피세포(border cell)는 근관(root cap)의 최외각 세포층에서 분화된 단일세포로서, 물리적인 힘을 가하면 예를 들어 근관의 근관 표피세포를 물로 씻어내면, 20∼25시간 이후에는 새로운 층의 근관 표피세포가 근관에 형성된다. 이 새로운 층의 완두 근관 표피세포가 형성되는 동안 근관 표피세포가 제거된 근관에서는 새로운 유전자 발현이 일어나고 있음이 mRNA differential display로 확인되었다. 즉, 이들 근관에서 새롭게 발현되는 유전자들의 일부는 근관 표피 세포의 분화에 관련되어 있다고 볼 수 있다. 또한, 단일 세포로 분화된 근관 표피세포에서는 독특한 유전자 발현이 일어나고 있음이 mRNA differential display로 확인되었다. 이 결과로 알 수 있는 것은 근관 표피세포는 다른 조직(잎, 줄기, 뿌리와 근관 표피세포가 제거된 근관)과는 다른 독특한 기능을 가졌다는 것이다.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1146-1151
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• 2005

The yeast Candida albicans has a distinguishing feature, dimorphism, which is the ability to switch between two morphological forms: a budding yeast form and a multicellular invasive filamentous form. This ability has been postulated to contribute to the virulence of this organism. Previously, we reported that the yeast-to-hypha transition in this organism is suppressed by farnesoic acid, a morphogenic autoregulatory substance that accumulates in the medium as the cells proliferate. In this study, using a differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique, we have identified several genes induced in C. albicans by farnesoic acid treatment. These observations indicate that farnesoic acid can alter the expressivity of multiple genes, including the DNA replication machinery and cell-cycle-control proteins.

• BMB Reports
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• 제29권3호
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• pp.272-275
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• 1996

The expression of genes induced by Dichloroacetate (DCA) treatment was analyzed by mRNA differential display. Purified total RNAs from rat liver treated with saline or DCA (100 mg/100 g b.w.) were reverse transcribed by using a set of oligonucleotide primers. The PCR products were resolved on a denaturing sequencing gel. PCR band representing mRNA expressed specifically in DCA-treated liver was excised and reamplified by PCR. A 120-bp c-DNA clone named IC1 was isolated and the DNA sequence of IC1 was analyzed. IC1 revealed 50% homology with 3' end of a mouse fibroblast growth factor mRNA This result indicates that DCA induces the expression of a gene which has a 50% homology with a Mouse fibroblast growth factor, and expression of this gene might be involved in non genotoxic process caused by DCA.

• Toxicological Research
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• 제25권1호
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• pp.35-40
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• 2009

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. Toxicity and carcinogenicity of TCDD is well established but the molecular mechanism is still poorly understood. Here, we found the noble responsive genes to TCDD using the differential display analysis. Treatment of HepG2 cells with TCDD showed a significantly different mRNA expression pattern from the untreated cells in differential display analysis. The differentially displayed bands were isolated and used as probes in dot blot and Northern blot analyses. Of thirty-five isolated differentially displayed bands, only two bands were confirmed as positive in dot blot and Northern blot analyses. The nucleotides sequences of these clones were analyzed and the search of Genebank database revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 92%) and the other is an unknown gene. RanBP2 is a nucleoporin with SUMO E3 ligase activity that functions in both nucleocytoplasmic transport and mitosis and its role as a novel tumor suppressor has been recently proposed. Thus, these results may suggest the clue elucidating the toxic mechanism of TCDD through RanBP2.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.307-313
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• 2003

파리지옥풀 first trap leaf에만 발현하는 유전자군을 탐색하기 위하여 기내배양 식물체와 포충능력을 가진 3년생 실생주을 이용하여 각각의 포충잎 (leaf base), 꽃조직 (flower tissue) 및 포충잎트랩 (first trap leaf)으로부터 분리한 RNA로 Fluorescent differential display (FDD)를 실시하였다. First trap leaf특이발현 유전자 15개를 screening하여 염기서열을 분석하였다. 분리된 DNA들은 protease inhibitor (Pl), myo-inositol-1-phosphate synthase 및 lipocalin-type prostaglandin D syn-thase 유전자들과 매우 유사하였다. 또한 Northern blot분석 결과, 이들 유전자들이 first trap leaf에 특이적으로 발현하고 있는 것을 확인하였다 FDD방법은 세포, 조직 및 기관에 특이적으로 발현하고 있는 유전자들을 선발하는데 매우 유용한 수단으로 사용될 수 있다.

• 대한전자공학회논문지SD
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• 제46권10호
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• pp.70-78
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• 2009

본 논문에서는 고속 저전압 차동 신호(Low-Voltage Differential Signaling, LVDS) 전송방식의 응용을 위한 전송선 분석 및 설계 최적화 방법을 제안한다. 차동 전송 경로 및 저전압 스윙 방법의 발전으로 인해 저전압 차동 신호 전송방식은 데이터 통신 분야, 고 해상도 디스플레이 분야, 평판 디스플레이 분야에서 매우 적은 소비전력, 개선된 잡음 특성 및 고속 데이터 전송률을 제공한다. 본 논문은 차동 유연성 인쇄 회로 보드(flexible printed circuit board, FPCB) 전송선에서 선 폭, 선 두께 및 선간격과 같은 전송선 설계 변수들의 최적화 기법을 이용하여 직렬 접속된 전송선에서 발생하는 임피던스 부정합과 신호 왜곡을 감소시키기 위해 개선 모델과 개발된 수식을 제안한다. 이러한 차동 FPCB 전송선의 고주파 특성을 평가하기 위해 주파수 영역에서 전파(full-wave) 전자기 시뮬레이션 및 시간 영역 시뮬레이션을 각각 수행하였다. 본 논문에서 제안하는 방법은 저전압 차동 신호 방식의 응용을 위한 고속 차동 FPCB 전송선을 최적화하는데 매우 도움이 되리라 믿는다.

• 한국유전체학회:학술대회논문집
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• 한국유전체학회 2003년도 The 12th Korea Genome Conference
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• pp.60.2-60.2
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• 2003

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1684-1690
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• 2005

Using mRNA differential display technique, we investigated differential gene expression in hybrids relative to their parents in a diallel cross involving four chicken breeds in order to provide an insight into the molecular basis of heterosis in chicken. The results indicated that there was extensive differential gene expression between chicken F1 hybrids and their parents which was classified into four kinds of patterns as following: (1) bands only detected in hybrid F1; (2) bands only absent in hybrid F1; (3) bands only detected in parent P1 or P2; (4) bands absent in parent P1 or P2. Forty-two differentially expressed cDNAs were cloned and sequenced, and their expression patterns were confirmed by Reverse-Northern dot blot. Sequence analysis and database searches revealed that genes showed differential expression between hybrid and parents were regulatory and functional genes involved in metabolism, mRNA splicing, transcriptional regulation, cell cycles and protein modification. These results indicated that hybridization between two parents can cause changes in expression of a variety of genes. In conclusion, that the altered pattern of gene expression in hybrids may be responsible for heterosis in chickens.

• BMB Reports
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• 제30권4호
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• pp.285-291
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• 1997

It is becoming increasingly evident that significant changes in gene expression occur during the course of neuronal differentiation. Thus, it should be possible to gain information about the biochemical events by identifying differentially expressed genes in neuronal differentiation The PC12 cell line is a useful model system to investigate the molecular mechanism underlying neuronal differentiation and has been used extensively for the study of the molecular events that underlie the biological actions of nerve growth factor (NGF). In this study, we report an application of the recently described mRNA differential display method to analyze differential gene expression during neuronal differentiation. Using this technique, we have identified several cDNA tags expressed differentially during neuronal differentiation. Interestingly, one of these clones was cytochrome c oxidase subunit I (COX I) gene. The differential expression of COX I gene was confirmed by Northern blot analysis as well as RT-PCR. Southern blot analysis of the genomic DNA of PC12 cells revealed that COX I is a single gene. Induction of the oxidative enzyme might reflect the energy requirement in neuronal differentiation.

• Journal of Plant Biotechnology
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• 제7권1호
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• pp.45-50
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• 2005

To clone blue and white flower color specific genes, mRNA differential display was carried out with two different Commelina species, C. communis Linne for blue color and C. coreana Leveille for. leucantha Nakai for white color. Fifty two and 100 cDNA clones specific for blue or white flower color, respectively, were ranging from 200 to 700 bp in size. From the reverse northern blot analysis, 12 and 7 positive clones were selected for blue and white flower, respectively. These clones appear to be novel cDNAs for these Commelina plants, but not color specific. This finding was supported by the northern blot analysis. However, two clones, B18 and B19, derived from blue flowered Commelina were highly expressed than in the white Commelina species, implying that further study will be valuable. The results indicated that both mRNA display experiment and dot blot analysis may not sensitive enough to clone color-determining gene from the plant, leading to explore more advanced method, like high-density colony array study (HDCA).