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Monitoring of Pathogenic Bacteria, Heavy Metals, and Pesticide Residues in Commercial Edible Dry Flowers (시판 23종 꽃차의 유해세균, 중금속 및 잔류농약 평가)

  • Lee, Yun-Seo;Lee, Dong-Hee;Hwang, Eun-Kyung;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.32 no.6
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    • pp.438-446
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    • 2022
  • Some flowers have a high sensual appeal owing to their unique shape, color, smell, and taste and have been used as functional food and oriental medicine. Recently, edible dry flowers (EDFs) have attracted social attention as noble sources of functional teas. In this study, for the risk assessment of EDFs, pathogenic bacteria, heavy metals, and pesticide residues were monitored in 23 types of commercial EDF. No Enterobacteria spp. and Listeria spp. were found in all EDF products. However, common aerobic bacteria (3.24~3.85 Log CFU/g) were found in EDF, namely, Pueraria lobata, Chamaemelum nobile, Acacia decurrens, Rhododendron mucronulatum Turcz, Oenothera lamarckiana, Brassica napus, and Prunus serrulata. Staphylococcus aureus was found in 11 and Salmonella sp. was found in 8 of the 23 EDFs. Considering the cold extraction of EDF for tea and beverages, the regulation of pathogenic bacteria in EDFs is necessary. No heavy metals such as Pb, Cd, Co, Cr, Cu, Ni, and As were found in all EDFs, except the dry flower of Hemerocallis fulva, which contained Pb at 0.08 ppm. Different pesticides and fungicides were found in EDFs, but their concentrations were very low (0.01~0.08 ppm) and below the maximal residue level. Only the dry flower of Chrysanthemum morifolium had a high content of chlorpyrifos (0.215 ppm), which is long-lasting pesticide. Our results suggest that the establishment of EDF regulations for pesticide residue, culture separation between edible and garden flowers, and guidelines for preventing pathogenic microbial contamination are necessary.

Effect of LED LightIrradiation on the Mycelial Growth and Fruit Body Development of Hypsizygus Marmoreus (LED 광원이 느티만가닥버섯 균의 균사 생장과 자실체 생육에 미치는 영향)

  • Kim, M.K.;Lee, Y.K.;Seo, G.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.1
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    • pp.99-112
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    • 2020
  • A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.

Component and Phase Analysis of Calcium Silicate Cement Clinker by Raw Materials Mix Design (원료 배합에 따른 칼슘 실리케이트 시멘트 클링커의 성분 및 상 분석)

  • Lee, Hyang-Sun;Song, Hun
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.3
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    • pp.251-258
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    • 2022
  • In the cement industry, in order to reduce CO2 emissions, technology for raw materials substitution and conversion, technology for improving process efficiency of utilizing low-carbon new heat sources, and technology for collecting and recycling process-generated CO2 are being developed. In this study, we conducted a basic experiment to contribute to the development of CSC that can store CO2 as carbonate minerals among process-generated CO2 capture and recycling technologies. Three types of CSC clinker with different SiO2/(CaO+SiO2) molar ratios were prepared with the clinker raw material formulation, and the characteristics of the clinker were analyzed. As a result of analysis and observation of CSC clinker, wollastonite and rankinite were formed. In addition, as a result of the carbonation test of the CSC paste, it was confirmed that calcite was produced as a carbonation product. The lower the SiO2/(CaO+SiO2) molar ratio in the CSC clinker chemical composition, the lower the wollastonite production amount, and the higher the rankinite production amount. And the amount of calcite production increased with the progress of carbonation of the CSC paste specimen. It is judged that rankinite is more reactive in mineralizing CO2 than wollastonite.

Flower Arrangement Study of Pine : Focusing on Pine Gracious Flower (소나무(松)의 화예적(華藝的) 고찰(考察) -송(松)의 문인화(文人花)를 중심(中心)으로-)

  • Kim, Jeong Min
    • Journal of the Korean Society of Floral Art and Design
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    • no.42
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    • pp.63-96
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    • 2020
  • The flower arrangement that human beings interact with the natural plants have characteristics according to their historical background and ethnicity and different aspects of culture related of the flower arrangements have been created by human inspiration and formative ability. The Korean flower arrangement is a product of civilization which originated in the natural environment of the four seasons. As background with the natural unity ideology, the Korean Flower Arrangement makes the life and philosophy of the naturalistic nature that flows in the mind of our nation to the relaxing beauty suited on cultures of ages by the Virtue, the Will, and the Vitality. The Literary Flower Arrangement is, based on the higher human personality and poetic sentiments as ideologies of our traditional oriental philosophy, the formative art made for aesthetic implications of philosophy by consisting plants' characters and tastes without expression of technical skills. In this study, focused on pine tree, in order to clarify that the flower arrangement of our spiritual culture is the Literary Flower Arrangement, make Identifying aesthetic characteristics through, the backgrounds and sources of being expressed by the Literary Flower Arrangement, poetic feelings, symbolism, metaphor, the formative form of flower arrangement, decoding of the flower arrangement genes, and the same time deduce the record of literary work and classify it each era, and establish the Literary Flower Arrangement theory which is the essence of Korean flower arrangement.

Exploring Fractional Ownership in Korean Art Market: Based on Business Model Canvas (분할소유 미술시장의 현황과 과제 - 비즈니스 모델 캔버스를 중심으로 -)

  • Lee, Yunjin;Koo, Jajoon
    • Korean Association of Arts Management
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    • no.58
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    • pp.179-204
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    • 2021
  • Not only the consumption trend after the COVID-19 pandemic but also low financial interest rates have stimulated people to invest artworks. With the recent noticeable growth, art investments that mainly conducted by younger generation through online platform can be characterized by a fractional ownership in art market which means several people share one piece of artwork. This study explores 4 fractional ownership platforms in the domestic art market including Art Together, Art & Guide, Tessa, and Pica projects, using a business model canvas that describes nine key elements: Customer Segments, Value Proposition, Channels, Customer Relationships, Revenue Streams, Key Resources, Key Activities, Key Partners and Cost Structure. The four cases have similar business models, but the details of revenue streams are different. The key sources of revenue are the profit and commission of the work. Thus, maximizing the profit margin of artworks is the core of revenue streams, so selecting and purchasing highly profitable artworks are significant. Based on the analysis, there are 3 suggestions to continue fractional ownership platform businesses in art market successfully. First, it is required to have a long-term perspective on art investments, as a way to diverse asset portfolio. Second, business confidence should be increased to maintain customer loyalty. Third, the role of platforms as competent experts is important.

Optimal Conditions for the Production of Gamma-aminobutyric Acid by Enterococcus casseliflavus PL05 Isolated from Oenanthe javanica

  • Choi, Se Mi;Kim, Jeong A;Kim, Geun Su;Kwon, Do Young;Kim, Sang Gu;Lee, Sang yun;Lee, Kang Wook
    • Journal of Food Hygiene and Safety
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    • v.37 no.1
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    • pp.21-28
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    • 2022
  • In this study, a new lactic acid bacterium (LAB) that could produce gamma-aminobutyric acid (GABA) was isolated from Oenanthe javanica (water celery) and identified as an Enteroccoccus casseliflavus strain. Until recently, there have been many studies on the gamma-aminobutyric acid producing lactic acid bacterium, as well as on some lactic acid bacterium in Enteroococcs genus, but none on the species E. casseliflavus. Therefore, in the purpose of finding the optimal conditions for GABA production of E. casseliflavus PL05, the effects of several conditions including the type of mediums, growth temperatures, initial pH, growth time, L-mono sodium glutamate (MSG) concentration, and carbon source were tested. The study revealed that the PL05 strain grew better in the Brain Heart Infusion (BHI) medium than in the Man, Rogosa, and Sharpe (MRS) or Tryptic Soy Broth (TSB) medium. Also, similar results were obtained with GABA production conditions. As a result of analysis on the GABA production yield by concentration of MSG, a GABA substrate, the highest production was found at 7% of MSG concentration. However, since similar level of production was found at 5%, it is considered to be more efficient to use 5% MSG concentration. The analysis on the growth and GABA production yield by carbon sources showed the highest results when maltose was used. From the final test under the optimal conditions found, 140.06±0.71 mM of GABA was produced over 24 hours with the conversion rate of 78.95%. Lastly, from the sensitivity analysis on the 10 different antibiotics, including vancomycin, it was found that there were not confirmed cases of resistance.

Effects of Ground Vegetation and Pyrethroid Spray on the Population Dynamics of Panonychus citri (Acari: Tetranychidae) and Natural Enemies in Citrus Orchard: A Short-term Effect (감귤원에서 초생관리와 합성피레스로이드계 조합처리가 귤응애와 천적의 발생양상에 미치는 단기효과)

  • Hyun, Seung Young;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.255-266
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    • 2022
  • This study was conducted to examine the effects of grass vegetation (W: manual weeding, NW: herbicide sprays) and pyrethroid spray (P: pyrethroid spray, NP: no pyrethroid spray) on the population dynamics of Panonychus citri and natural enemies in citrus orchards. Two essential hypothesis were made to test the population dynamics: 1) weed planting promotes natural enemies by offering habitat and alternative food sources, resulting in the reduction of P. citri populations, and 2) pyrethroid spray removes natural enemies by its non-selective toxicity, resulting in the increasement of P. citri populations. The observed natural enemy populations (mainly Phytoseiids and Agistemus sp.) were not different largely from the expected values in the hypothesis, which assumes more abundant natural enemies in weeds and no pyrethroid plots. Although some discrepancy was occurred in NW+NP and W+NP plots in 2011, the observed values were almost same with expected values in 2012. In overall, pesticide effect was strongly significant and pyrthroids removed largely natural enemies. Although habitat (weeds) effect showed a conflict result, natural enemy population increased in plots allowing weed growth, when considering the increased autumn population relatively compared to that of spring-summer population. The decreased abnormal P. citri populations in pyrethroid plots could be explained under the assumption of a strong repellent behavior of P. citri to the pyrethroids.

Analysis of Gene Diversity and Phenetic Relationship of Water Dropwort Species in Korea Using RAPD (OPB Primers) Markers (한국 미나리 집단에 대한 RAPD (OPB 프라이머)에 의한 유전적 다양성과 표현형 관계)

  • Huh, Man Kyu
    • Journal of Life Science
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    • v.32 no.8
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    • pp.595-600
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    • 2022
  • Water dropworts, Oenanthe javanica and O. javanica var. japonica are called "minari" in Korea and are eaten as a vegetable. Cicuta virosa is common European water hemlock and has toxic properties, such as cicutoxin. Molecular variations of water dropwort species in Korea were investigated using random amplified polymorphic DNA (RAPD). The six populations were studied with 10 primers (Operon, OPB) for RAPD analysis. The 72 DNA fragments (bands) were found among six populations. Among these 72 bands, 61 (84.7%) bands were polymorphic. The typical populations of Cicuta virosa in Korea were small, isolated, and patchily distributed for natural populations and they maintained a high level of genetic diversity. However, when cultivated populations of O. javanica var. japonica were large and widely grown in rice paddies as vegetables, they maintained a lower genetic diversity than those of C. virosa and wild populations of O. javanica. Although the diversity indices of wild populations were shown to be higher than those of cultivated populations, no significant difference for measures of genetic variability was shown. Total genetic diversity value (HT) was 0.342. The interlocus variation in the within-population genetic diversity (HS) was 0.201. The proportion of total genetic variation due to differences among populations (GST) range was 0.414, indicating that 41.4% of the total variation was among populations. In conclusion, the RAPD technique was a useful method for discrimination between C. virosa and O. javanica. In addition, RAPD-OPB markers could further distinguish the strains from different food sources.

Evaluation of Characteristics of Sludge generated from Active Treatment System of Mine Drainage (광산배수의 적극적 처리시설에서 발생하는 슬러지 특성 평가)

  • Jung-Eun Kim;Won Hyun Ji
    • Economic and Environmental Geology
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    • v.56 no.4
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    • pp.409-419
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    • 2023
  • Acid mine drainage(AMD) treatment is classified as both passive and active treatment. During the treatment, about 5,000 tons of neutralization sludge is generated as a by-product per year in Korea. This study was conducted to evaluate the characteristics of sludge generated from physico·chemical treatment processes as an active treatment from 5 different sources (D, H, S, T, Y) and the possibility of the sludges being recycled. The sludges have a pH range of 5.86 ~ pH 7.89, and a water content range of 51% ~ 82%. Most of particle sizes were less than 25 ㎛. In analysis of inorganic elements, the concentration of Al, Fe, and Mn were between 1,189 mg/kg ~ 129,344 mg/kg, 106,132 mg/kg ~ 338,011 mg/kg, and 3,472 mg/kg ~ 11,743 mg/kg, respectively. The concentration of As and Zn in sludge-T, Cd in sludge-D, Ni in sludge-H, Zn in sludge-S, and Cd in sludge-Y exceeded the soil contamination standards of Korea. The results from 2 separate kinds of leaching test, the Korea Standard Leaching Test(KSLT) and Toxicity Characteristic Leaching Procedure(TCLP), showed that all the sludges met the Korea groundwater standards. From the XRD and SEM-EDS analysis, the peaks of calcite and quartz were found in the sludges. The sludge also had a high proportion of Fe and O, and the majority of the composition was amorphous iron hydroxide.

Exploring the Reliability of an Assessment based on Automatic Item Generation Using the Multivariate Generalizability Theory (다변량일반화가능도 이론을 적용한 자동문항생성 기반 평가에서의 신뢰도 탐색)

  • Jinmin Chung;Sungyeun Kim
    • Journal of Science Education
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    • v.47 no.2
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    • pp.211-224
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    • 2023
  • The purpose of this study is to suggest how to investigate the reliability of the assessment, which consists of items generated by automatic item generation using empirical example data. To achieve this, we analyzed the illustrative assessment data by applying the multivariate generalizability theory, which can reflect the design of responding to different items for each student and multiple error sources in the assessment score. The result of the G-study showed that, in most designs, the student effect corresponding to the true score of the classical test theory was relatively large after residual effects. In addition, in the design where the content domain was fixed, the ranking of students did not change depending on the item types or items. Similarly, in the design where the item format was fixed, the difficulty showed little variation depending on the content domains. The result of the D-study indicated that the original assessment data achieved a sufficient level of reliability. It was also found that higher reliability than the original assessment data could be obtained by reducing the number of items in the content domains of operation, geometry, and probability and statistics, or by assigning higher weights to the domains of letters and formulas, and function. The efficient measurement conditions presented in this study are limited to the illustrative assessment data. However, the method applied in this study can be utilized to determine the reliability and to find efficient measurement conditions for the various assessment situations using automatic item generation based on measurement traits.