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Effect of Energy Sources (Glucose, Pyruvate and Lactate) Added to Dulbecco's Modified Eagle Medium (DMEM) on the Mouse 2-cell Embryo Development (Glutamine 함유 배양액에 첨가한 에너지원이 마우스의 배 발달에 미치는 영향)

  • Kim, Ju-Hwan;Park, Kee-Sang;Lee, Taek-Hoo;Chun, Sang-Sik;Song, Hai-Bum
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.1
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    • pp.1-7
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    • 2000
  • Objective: Mammalian embryos undergo changes of energy environment for transfer from oviduct to uterus. Also, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration (oviduct - glucose: 0.5 mM, pyruvate: 0.32 mM, lactate: 10.5 mM; uterus - goucose: 3.15 mM, pyruvate: 0.1mM, lactate: 5.87 mM, respectively). This study was conducted to examine the effect of these energy sources added in DMEM with glutamine on the mouse embryo development. Methods: There was used ICR female mouse. Two cell embryos of mouse are collected by method of 'flushing'. Flushing fluid was used Ham's F-10 added to 20% FBS. The collected 2 cell embryos were cultured in media such as Control (only DMEM), group A and B (DMEM supplemented with 0.5 mM and 3.15 mM glucose), and group C and D (DMEM supplemented with 0.1 mM and 0.32 mM pyruvate), and group E and F (DMEM supplemented with 5.87 mM and 10.5 mM lactate). All experimental media supplemented with 20% hFF, respectively. Pattern of embryo development was observed to interval at 24hr during 96hr. Results : The media with glutamine added glucose (group A: 51.0%; group B: 48.4%) was significantly (p<0.05) higher than other experimental group in development into the morula stage after 24 hr in culture, but not significantly different compared with control and the rate of development into the blastocyst was significantly (p<0.05) low in the both of pyruvate (group C: 7.9% group D: 6.8%) and lactate (group E: 7.1%, group F: 7.1%) treatment group after 48 hr in culture. Development into the blastocyst and hatched balstocyst after 72 hr in culture revealed similarly in control (81.9%) and glucose treatment group (group A: 83.3%, group B: 82.8%). However, development into the hatched and attached blastocyst after 96hr in culture revealed significantly (p<0.05) development in the glucose treatment group (group A: 82.3%, group B: 78.5%) than control (63.2%), and its of pyruvate (group C: 34.1%, group D: 34.1%) and lactate (group E: 25.9%, group F: 33.3%) treatment group were significantly (p<0.05) lower than control similar to previous observations. Conclusion : The glucose added to the DMEM with only glutamine, as energy source, was highly to the rate of development compared with control, but the other energy sources were not, synthetically. Above refer to, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration. Thus, further studies are will examine continuously to effects by interaction of different energy sources in the mouse embryo development, and these results will provide to foundation on the human embryo culture.

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Experiment on the Cooling Characteristics of Protruding Heat Sources in a Channel (채널 내 돌출된 열원의 냉각특성 실험)

  • 신지영;손영석;김상민
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.16 no.9
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    • pp.788-795
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    • 2004
  • Cooling characteristics of protruding heat sources in a parallel channel with or without baffles are studied experimentally, The test section consists of two channels formed by two covers and one board made of polycarbonate which has three uniform heat source blocks. Five different cooling methods are considered to find out the most efficient cooling method in a given geometry and heat sources. The velocity and temperature of cooling medium, the temperature of the block surface are measured. The results are compared to examine the cooling characteristics of the different cooling methods.

Dynamic Paralleling Behaviors of High Power Trench and Fieldstop IGBTs

  • Wu, Yu;Sun, Yaojie;Lin, Yandan
    • Journal of Power Electronics
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    • v.14 no.4
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    • pp.788-795
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    • 2014
  • This paper demonstrates the dynamic behaviors of paralleled high power IGBTs using trench and fieldstop technologies. Four IGBTs are paralleled and standard deviation is adopted to represent the imbalance. Experiments are conducted under three different operation conditions and at different temperatures ranging from $-25^{\circ}C$ to $125^{\circ}C$. The experimental results show that operation at very low and very high temperatures usually aggravates the switching behaviors. There is a trade-off between the balance and the losses at low temperatures. These results can help in the design of heat sinks in paralleling applications confronting very low temperatures.

The Relationship between the Type of New Product Idea Sources and the Rate of Success of NEW Product Development and Commercialization (신제품 아이디어 창출유형과 개발 및 사업화 성패에 관한 연구)

  • 홍종원;용세중
    • Journal of Technology Innovation
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    • v.2 no.1
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    • pp.219-241
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    • 1994
  • This paper presents the results of an empirical study on the relationship between the type of new product idea sources and the rate of success of new product development and commercialization in Korean industries. The sample was taken from various Korean industries including telecommunication, electronics, chemical, machinery, textile, etc. and composed of 45 failure and 73 success cases. The findings are not much different from those of previous studies done in the developed countries, but show some typical characteristics of new product development activities and the idea sources, information acquisition methods, type of information , incentives for idea generation, etc. of the firms in developing country like Korea. Especially the survival rate curve and the accumulative investment curve from the idea generation to commercialization phase show a little different behavior from the previous study, which also reflect the manpower, duration of each phase of the new product development process in the Korean firms.

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Effect of High Dietary Fiber Diet on Lead Absorption and Metabolic Changes in Growing Rats (종류가 다른 고 섬유질식이가 흰쥐의 납 흡수 및 체내대사에 미치는 영향)

  • 김미경
    • Journal of Nutrition and Health
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    • v.23 no.7
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    • pp.492-503
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    • 1990
  • This study was performed to investigate nutritional effect of various dietary fibers on lead absorption, and protein and lipid metabolisms in growing rats. Sixty male rats of Sprague-Dawley strain weighing 140$\pm$1.1g were blocked into 10 groups according to body weight and fed 10 kinds of diet different with fiber sources [non-fiber, cellulose, pectin, guar gum or carboxymethylcellulose(CMC)] and lead levels (0 or 1%) for 4 weeks. Results were summerized as follows : 1) Food intake, weight gain, FER and PER were remarkably decreased in lead(Pb)-added groups. Weight gain, FER and PER in Pb-added pectin group were significantly lower than those in Pb-added non-fiber group. 2) Liver and kidney weights, femur weight and length, hematocrit and hemoglobin content were decreased in Pb-added groups. Especially femur and liver weights in pectin groups were the lowest among groups. 3) Total protein content in serum was significantly decreased in Pb-added groups but was not different with dietary fiber sources. Total lipid content in serum was not different with dietary Pb levels and fiber sources, but cholesterol content in serum of guar gum group was significantly decreased by Pb addition. 4) Nitrogen, lipid and cholesteol contents in liver were significantly decreased in Pb-added groups, and lipid content in liver of pectin and CMC groups was lower than other groups. 5) Daily urinary and fecal excretions of nitrogen, kipid and cholesterol were decreased in Pb-added groups, and fecal nitrogen was significantly increased in Pb-added groups, and fecal nitrogen of cellulose and guar gum groups was significantly higher than other groups. Fecal excretions of lipid and cholesterol were increased by dietary fibers, and especially fecal lipid was remarkably increased in pectin and guar hum group. 6) Pb contents in liver and femur were decreased by dietary fibers. Especially Pb contents in liver, kidney and femur were significantly decreased in guar gum group. 7) Daily urinary and fecal excretions of Pb were significantly increased in cellulose and guar gum groups, and fecla excretion of Pb in guar gum group was twice of non-fiber group. Pb absorption ratio was significantly decreased in guar gum group. In conclusion, dietary fibers have effect on protein and lipid metabolisms, and decreased intestinal absorption of Pb by increasing fecal excretion. But the degree of effect was different with dietary fiber sources.

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Fluorescence Properties of Size Fractions of Dissolved Organic Matter Originated From Different Sources (생성 기원에 따른 용존 자연유기물질 분자량별 형광특성 비교)

  • Hur, Jin;Park, Min-Hye
    • Journal of Korean Society on Water Environment
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    • v.23 no.4
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    • pp.482-489
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    • 2007
  • Fluorescence properties and carbohydrate content were investigated using ultrafiltrated size fractions of dissolved organic matters (DOM) originated from different sources. The materials included a treated sewage, an algal organic matter, and a soil leachate, all of which are major constituents of dissolved organic matter in a typical urban river. Four different size fractions were separated from the three sources of each DOM. The size distribution demonstrated that a higher molecular weight fraction was more present in soil leachate compared to two other source DOMs. A higher content of carbohydrates was observed in the following order - algal DOM > treated sewage > soil leachate. A wide range of specific UV absorbance was observed from size fractions of a single source DOM, indicating that aromatic carbon structures are heterogeneously distributed within one source of DOM. The structural heterogeneity was the most pronounced for the soil leachate. The fluorescence index ($F_{450}/F_{500}$) of the treated sewage was similar to that (2.0) typically obtained from autochthonous DOM, suggesting that the treated sewage exhibited autochthonous organic matter-like properties. No protein-like fluorescence intensities were observed for all of the soil leachate size fractions whereas they were observed with two other source DOMs. Based upon the fluorescence peak ratios from fluorescence excitation-emission matrix (EEM), two discrimination indices could be suggested to distinguish three different source DOMs. It is expected that the suggested discrimination indices will be useful to predict the sources of DOM in a typical urban river affected by treated sewage.

The Effect of Sesame Oil, Perilla Oil and Beef Tallow on Body Lipid Metabolism and Immune Response (한국에서 상용되는 식용유지로 사육된 흰쥐의 체내 지방대사 및 면역능력에 대한 연구)

  • 김우경
    • Journal of Nutrition and Health
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    • v.22 no.1
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    • pp.42-53
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    • 1989
  • The research was designed to study the effect of different fat sources and levels on Body lipid metabolizm and immune responses in Sprague-Dawely strain male rats. These effect of different fat sources compared with sesame Oil, Perilla oil and Beet tallow. Fat sources were divided into 3 groups respectively 7%, 15%, 30% fat level on diet weight basis. The experimental period was 54days. 1) The body weight gain was significantly low in NF group. In Sesame oil group and perilla oil group, low fat level groups were higher than medium, high fat level groups. But in Beef tallow group, high fat level groups were higher than low and Medium groups. 2) The weight of liver, kidney and epididymal fat ped tend to increse with increasing body weight. 3) The contents of triglyceride and total lipid in serum were significantly different with dietary fat sources and Perilla oil group was the lowest. 4) The contents of triglyceride and total lipid in liver were significantly different with dietary fat levels and high fat level group was higher than low fat level group. 5) Perilla oil group, compared with Beef tallow group, showed the higher excretion of cholesterol through feces and the higher deposit of cholesterol in liver. Therefore serum cholesterol level of Perilla oil group was lower than that of Beef tallow group. But eventhough Sesame oil is vegetable oil, Sesame oil did not showed an effect like Perllia oil on serum cholesterol level. 6) Weight of thymus decreased with fat levels particularly in vegetale oil. And it had on effect on mitogen response, mitogen response decreased with fat level in vegetable oil. But in Beef tallow, there was no difference in fat level.

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Intraspecific diet shifts of the sesarmid crab, Sesarma dehaani, in three wetlands in the Han River estuary, South Korea

  • Yang, Dongwoo;Han, Donguk;Park, Sangkyu
    • Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.31-42
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    • 2019
  • Background: Han River estuary is a national wetland reserve near the Demilitarized Zone (DMZ) between South Korea and North Korea. This trans-boundary estuary area has been well preserved and shows distinctive plant communities along the salinity gradient. To elucidate energy flows and nutrient cycling in this area, we studied trophic relations between the dominant sesarmid crab, Sesarma dehaani, and food sources in three wetlands with different environments along the estuarine gradients. Results: Stable isotope signatures (${\delta}^{13}C$ and ${\delta}^{15}N$) of the crabs were significantly different among the sites and body size classes. Seasonal changes in ${\delta}^{13}C$ of small crabs were distinct from those of large individuals at all the sites. The isotopic values and fatty acid profiles of the crabs were more different among the sites in September than in May. In May, large-sized crabs utilized more plant materials compared to other dietary sources in contrast to small-sized crabs as revealed by a stable isotope mixing modeling, whereas contributions to diets of crabs were not dominated by a specific diet for different body size in September except at site 1. Based on PCA loadings, fatty acid content of $18:3{\omega}3$, known as a biomarker of plant materials, was the main factor to separate size groups of crabs in May and September. The ${\delta}^{13}C$ value of sediment had high correlation with those of small-sized crabs at site 1 and 2 when 1-month time lag was applied to the value for crabs during the surveyed period. Conclusions: Based on the stable isotope and fatty acid results, the consumption habits of S. dehaani appear to be distinguished by sites and their size. In particular, smaller size of S. dehaani appears to be more dependent on fewer food sources and is influenced more by the diet sources from the sediments in Han River estuary.

Enhancing Recommender Systems by Fusing Diverse Information Sources through Data Transformation and Feature Selection

  • Thi-Linh Ho;Anh-Cuong Le;Dinh-Hong Vu
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.5
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    • pp.1413-1432
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    • 2023
  • Recommender systems aim to recommend items to users by taking into account their probable interests. This study focuses on creating a model that utilizes multiple sources of information about users and items by employing a multimodality approach. The study addresses the task of how to gather information from different sources (modalities) and transform them into a uniform format, resulting in a multi-modal feature description for users and items. This work also aims to transform and represent the features extracted from different modalities so that the information is in a compatible format for integration and contains important, useful information for the prediction model. To achieve this goal, we propose a novel multi-modal recommendation model, which involves extracting latent features of users and items from a utility matrix using matrix factorization techniques. Various transformation techniques are utilized to extract features from other sources of information such as user reviews, item descriptions, and item categories. We also proposed the use of Principal Component Analysis (PCA) and Feature Selection techniques to reduce the data dimension and extract important features as well as remove noisy features to increase the accuracy of the model. We conducted several different experimental models based on different subsets of modalities on the MovieLens and Amazon sub-category datasets. According to the experimental results, the proposed model significantly enhances the accuracy of recommendations when compared to SVD, which is acknowledged as one of the most effective models for recommender systems. Specifically, the proposed model reduces the RMSE by a range of 4.8% to 21.43% and increases the Precision by a range of 2.07% to 26.49% for the Amazon datasets. Similarly, for the MovieLens dataset, the proposed model reduces the RMSE by 45.61% and increases the Precision by 14.06%. Additionally, the experimental results on both datasets demonstrate that combining information from multiple modalities in the proposed model leads to superior outcomes compared to relying on a single type of information.

Association Between Protein Intake From Different Animal and Plant Origins and the Risk of Non-Alcoholic Fatty Liver Disease: A Case-Control Study

  • Yasaman Khazaei;Narges Dehghanseresht;Sara Ebrahimi Mousavi;Matin Nazari;Shekoufeh Salamat;Omid Asbaghi;Anahita Mansoori
    • Clinical Nutrition Research
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    • v.12 no.1
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    • pp.29-39
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    • 2023
  • Previous studies have frequently reviewed how different macronutrients affect liver health. Still, no study centered around protein intake and the non-alcoholic fatty liver disease (NAFLD) risk relationship. This study aimed to examine the association between the consumption of total and different sources of protein and NAFLD risk. We allocated 243 eligible subjects to the case and control groups, including 121 incidence cases of NAFLD, and 122 healthy controls. Two groups were matched in age, body mass index, and sex. We evaluated the usual food intake of participants using FFQ. Binary logistic regression was conducted to estimate the risk of NAFLD in relation to different sources of protein intake. The age of participants was 42.7 years on average, and 53.1% were male. We found Higher intake of protein in total (odds ratio [OR], 0.24; 95% confidence interval [CI], 0.11-0.52) was significantly associated with a lower risk of NAFLD, despite adjusting for multiple confounders. in detail, higher tendency to the vegetables (OR, 0.28; 95% CI, 0.13-0.59), grains (OR, 0.24; 95% CI, 0.11-0.52), and nuts (OR, 0.25; 95% CI, 0.12-0.52) as the main sources of protein, were remarkably correlated with lower NAFLD risk. In contrary, increased intake of meat protein (OR, 3.15; 95% CI, 1.46-6.81) was positively associated with a higher risk. Totally, more calorie intake from proteins was inversely associated with lower NAFLD risk. This was more likely when the protein sources were selected less from meats and more from plants. Accordingly, increasing the consumption of proteins, particularly from plants, may be a good recommendation to manage and prevent NAFLD.