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Studies on Methanol-assimilating Yeasts (메탄올 자화효모에 관한 연구)

  • 전순배
    • Korean Journal of Microbiology
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    • v.19 no.4
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    • pp.163-173
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    • 1981
  • The distribution of methanol-assimilating yeasts on three different sources (elm bark, soil and fresh-water mud) and the growth conditions of a new strain of Candidaboidinii (SIO) wereexamines. From 150 samples, 91 methanol yeasts were isolated through enrichment culture ; they were identified as 77 strains of Candida boidinii including four new strains, 5 isolates of Torulopsis pinus, 3 strains of Hansenula polymorpha and one sstrain of Pichia pastoris respectively. The comparison of these yeasts with three sources indicated that decaying bark of elm tree other two, and that Gandida boidinii was most frequently distributed in all three sources. Four new strains of Candida boidinii were freshly isolated and their taxonomical properties were discussed. Of them, SIO strain was selected and characterized for its growth on methanol. This yeast could grow well on less than 1%(v/v) methanol. However, its growth was inhibited at 10% methanol. The cell yield was 3.1g (dry weight) per 1000ml of mineral mediurr, containing 1%(v/v) methanol as well as 01.% yeast extract as additive. The concentration of 0.1% yeast extract appears to be effective for the biomass production. Optimum conditions for growth on methanol was found to be : $28^{\circ}C,\;NH_4^+$ as nitrogen sources, thiamine as vitamin, and pH 4.5 to 6.0. The cell composition was as follows : crude protein and nucleic acids were 54% and 7% respectively. The amino acids were also described.

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NEAR-IR POLARIMETRY AROUND 30 DORADUS: I. SEPARATION OF THE GALACTIC SOURCES

  • Kim, Jae-Yeong;Pak, Soo-Jong;Choi, Min-Ho;Kang, Won-Seok;Kandori, Ryo;Tamura, Motohide;Nagata, Tetsuya;Kwon, Jung-Mi;Kato, Daisuke;Jaffe, Daniel T.
    • Journal of The Korean Astronomical Society
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    • v.44 no.4
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    • pp.135-142
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    • 2011
  • A $200'{\times}200'$ region around 30 Doradus in the Large Magellanic Cloud (LMC) is observed and analyzed in the near-infrared. We obtain polarimetry data in the J, H, and Ks bands using the SIRIUS polarimeter SIRPOL at the Infrared Survey Facility 1.4 m telescope. We measure the Stokes parameters of 2562 point-like sources to derive the degree of polarization and the polarization position angles. We discuss the statistics of the groups classified by color-magnitude diagram and proper motions of the sources, in order to separate the Galactic foreground sources from those present in the LMC. We notice that groups classified by the proper motion data show a tendency towards different polarimetric properties.

Comparison of the Purchase Criteria and Fashion Information Sources for the Middle-aged and Elderly Women's Fashion Markets Segmented Based on Benefits Sought (의복추구혜택에 따른 중.노년기 여성 세분시장의 구매기준 및 패션정보원 비교분석)

  • Lee, Jin-Hwa;Kim, Chil-Soon
    • Journal of the Korean Home Economics Association
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    • v.45 no.5
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    • pp.39-49
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    • 2007
  • The purpose of this study was 1) to segment the middle-aged and elderly women's fashion market based on the clothing benefits sought by the buyer and 2) to compare the purchase criteria and fashion information sources among the segmented markets. The data were collected using a self-administered questionnaire in Seoul and its surrounding suburban areas. Factor analysis, ANOVA, Duncan test, and Dunnett's T3 tests were used to conduct the data analysis from 285 out of 300 questionnaires. The middle-aged and elderly women's fashion market was segmented into four groups; value-oriented, social status/trend-oriented, uniqueness-oriented, and protection/ convenience-oriented. All four groups were significantly different in terms of purchase criteria and fashion information sources. The social status/trend-oriented group used external purchase criteria, such as country of origin or brand and obtained fashion information from personal experience, advice from the salesperson, and celebrities. The protection/convenience-oriented group sourced fashion information from newspapers, the internet, and the radio. The uniqueness-oriented group put less importance on practical use/convenience criteria. Marketing strategies for these segmented markets were discussed.

Estimation of the Number of Sources Based on Hypothesis Testing

  • Xiao, Manlin;Wei, Ping;Tai, Heng-Ming
    • Journal of Communications and Networks
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    • v.14 no.5
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    • pp.481-486
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    • 2012
  • Accurate and efficient estimation of the number of sources is critical for providing the parameter of targets in problems of array signal processing and blind source separation among other such problems. When conventional estimators work in unfavorable scenarios, e.g., at low signal-to-noise ratio (SNR), with a small number of snapshots, or for sources with a different strength, it is challenging to maintain good performance. In this paper, the detection limit of the minimum description length (MDL) estimator and the signal strength required for reliable detection are first discussed. Though a comparison, we analyze the reason that performances of classical estimators deteriorate completely in unfavorable scenarios. After discussing the limiting distribution of eigenvalues of the sample covariance matrix, we propose a new approach for estimating the number of sources which is based on a sequential hypothesis test. The new estimator performs better in unfavorable scenarios and is consistent in the traditional asymptotic sense. Finally, numerical evaluations indicate that the proposed estimator performs well when compared with other traditional estimators at low SNR and in the finite sample size case, especially when weak signals are superimposed on the strong signals.

Mathematical Models on Diffusive Loss of Non-Aqueous Phase Organic Solvents from a Disk Source (디스크소스로부터 NAPL의 확산손실에 관한 수학적 모델)

  • Yoon, In-Taek;S.E., Dickson
    • Journal of Soil and Groundwater Environment
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    • v.13 no.6
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    • pp.40-49
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    • 2008
  • Matrix diffusion from planar fractures was studied mathematically and through physical model experiments. Mathematical models were developed to simulate diffusion from 2D and 3D instantaneous disk sources and a 3D continuous disk source. The models were based on analytical solutions previously developed by Carslaw and Jaeger (1959). The mathematical simulations indicated that the 2D scenario produces significantly different results from the 3D scenario, the time for mass disappearance is significantly larger for continuous sources than for instantaneous sources, the normalized concentration generally decreased over time for instantaneous sources while it increased over time for continuous sources, diffusion rates decrease significantly over time and space, and the normalized mass loss from the source zone never reaches 1 for continuous sources due to the semi-infinite integral. The simulations also showed that disappearance times increase exponentially with increasing source radii and matrix porosity, and decrease with increasing aqueous-phase NAPL solubilities.

Preliminary Source Apportionment of Ambient VOCs Measured in Seoul Metropolitan Area by Positive Matrix Factorization (PMF를 이용한 수도권지역 VOCs의 배출원 추정)

  • Han J. S.;Moon K. J.;Kim R. H.;Shin S. A.;Hong Y. D.;Jung I. R.
    • Journal of Korean Society for Atmospheric Environment
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    • v.22 no.1
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    • pp.85-97
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    • 2006
  • The PAMS data collected at four sites in Seoul metropolitan area in 2004 were analyzed using the positive matrix factorization (PMF) technique, in order to identify the possible sources and estimate their contributions to ambient VOCs. Ten sources were then resolved at Jeongdong, Bulgwang, Yangpyeong, and Seokmo, including vehicle exhaust, LPG vehicle, petroleum evaporation, coating, solvent, asphalt, LNG, Industry & heating, open burning, and biogenic source. The PMF analysis results showed that vehicle exhaust commonly contributed the largest portion of the predicted total VOCs mass concentration, more than $30\%$ at four sites. The contribution of other resolved sources were significantly different according to the characteristics of site location. In the case of Jeongdong and bulgwang located in urban area, various anthropogenic sources such as coating, solvent, asphalt, residual LPG, and petroleum evaporation contributed about $40\%$ of total VOCs mass. On the other hand, at yangpyeong and Seokmo located in rural and remote area, the portion of these anthropogenic sources was reduced to less than $30\%$ and the contribution of natural sources including open burning and biogenic source clearly observed. These results were considerably corresponding to the emission inventory investigated in this region.

A Study on the Pollution Sources of Simple water Supply Piped System using Statistical Analysis (통계적 분석을 이용한 간이급수시설의 오염원에 관한 연구)

  • 이홍근;김현용;백도현;김지영;이태호
    • Journal of environmental and Sanitary engineering
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    • v.14 no.2
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    • pp.56-67
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    • 1999
  • This study was performed to suggest the basic data and plans for the establishment of safe water supply plans in simple water supply piped system in the rural areas. In 4 different places, 24 points of water sources 36 points of taps from water sources were sampled. Of the whole 60 points, 55 points were ground water and 5 points were surface water. 14 items were measured for the analysis of water quality on each samples. The measured items were analyzed again by statistical method ; cluster analysis and principle components analysis. The results of this study are as followed. 1) In water quality analysis on water sources, 4 items, bacteria, E.coli, NH3-N and Fe exceed the standard. Of 24 points, 20 points(83%) on bacteria, 1 point(4%) on NH3-N and Fe exceed the standard. 2) In water quality analysis on near and remote taps, 4 items, bacteria, E.coli, NH3-N and Fe , exceed the standard. Of 36 points, 20 points (81%) on bactria, 1 pint(3%) on NH3-N and Fe exceed the standard. 3)Cluster analysis on water quality shows the differences by the kinds of water sources, geographical characteristics and distance from water sources. 4) Principle components analysis on ground water shows that Factor 1 and Factor 3 are natural fluctuation by the content of soil. Also, Factor 2 and Factor 4 are penetration of pollutants to underground. Therefore, it is needed to take deeper ground water in order to prevent from pollution in the areas which have ground water as water source . 5) Principle components analysis on surface water shows that Factor 1 is penetration of vacteria from surface to water source when rainfalls. Also, Factor 2 is fluctuation of water quality by the geographical characteristics. Therefore, the counterplans against non-point pollution source must be taken. Filtration and disinfection facilities are needed in the areas which have surface water as water source.

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A Useful Material Production from Whey : Effect of Carbon Sources on Zooglan Production by Zoogloea ramigera (유청으로부터 유용물질 생산 : Zoogloea remigera에 의한 Zooglan 생산에서 탄소원의 영향)

  • 김동운;이재찬
    • KSBB Journal
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    • v.10 no.2
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    • pp.221-229
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    • 1995
  • Effects of carbon sources on zooglan production by Zoogloea ramigera were investigated. The production of zooglan was varied according to the carbon sources used. The largest amount of zooglan was obtained when lactose was used as carbon source and fermentation broth with lactose showed the higher viscosity. The effects of carbon sources were in decreasing order of lactose, glucose, galactose and sucrose. The viscosities of purified zooglan solutions (5g/L) obtained from different carbon sources were measured. When lactose was used, the viscosities of zooglan solutions was quite high and other carbon sources such as glucose and galactose gave little lower viscosities than lactose but sucrose gave very low values. On the other hand, it could be postulated that most of lactose is hydrolyzed by intracellular ${\beta}$-galactosidase.

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Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production

  • Kim, Garam;Mujtaba, Ghulam;Lee, Kisay
    • ALGAE
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    • v.31 no.3
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    • pp.257-266
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    • 2016
  • Nitrogen is one of the most critical nutrients affecting cell growth and biochemical composition of microalgae, ultimately determining the lipid or carbohydrate productivity for biofuels. In order to investigate the effect of nitrogen sources on the cell growth and biochemical composition of the marine microalga Tetraselmis sp., nine different N sources, including NaNO3, KNO3, NH4NO3, NH4HCO3, NH4Cl, CH3COONH4, urea, glycine, and yeast extract were compared at the given concentration of 8.82 mM. Higher biomass concentration was achieved under organic nitrogen sources, such as yeast extract (2.23 g L−1) and glycine (1.62 g L−1), compared to nitrate- (1.45 g L−1) or ammonium-N (0.98 g L−1). All ammonium sources showed an inhibition of cell growth, but accumulated higher lipids, showing a maximum content of 28.3% in ammonium bicarbonate. When Tetraselmis sp. was cultivated using yeast extract, the highest lipid productivity of 36.0 mg L−1 d−1 was achieved, followed by glycine 21.5 mg L−1 d−1 and nitrate 19.9 mg L−1 d−1. Ammonium bicarbonate resulted in the lowest lipid productivity of 14.4 mg L−1 d−1. The major fatty acids in Tetraselmis sp. were palmitic, oleic, linoleic and linolenic acids, regardless of the nutritional compositions, indicating the suitability of this species for biodiesel production.

Comparison of Inoculation Effects for Different Seed and Nodule Sources in Alnus firma Grown in Yeosu Industrial Complex(I) (여수산업단지에서 자라는 사방오리나무에서 채취한 종자와 질소고정 뿌리혹의 접종 효과 비교(I))

  • Su-Young Woo;Oh-Kyu Kwon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.4 no.1
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    • pp.12-17
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    • 2002
  • This study identified the physiological characteristics of Alnus firma seedlings collected from air polluted industrial complex of Yeosu area with inoculation of nitrogen-fixing nodule. A. firma individuals resistant or sensitive to air pollution stress at the study area were selected. Seeds of the individuals were sown in a pot and inoculated with the nodules collected at the same area about 3 weeks after germination. To compare the inoculation effects, photosynthesis was measured among the A. firma seedlings under 12 inoculation combinations of seed sources and nodule sources. Photosynthetic activities of seedlings from resistant mother trees were obviously higher than those from sensitive mother trees. In general, the seedlings inoculated with nodule of sensitive trees, showed a worse physiological trend on every combination. In this study, inoculation effect was less important factor than seed sources to photosynthetic activities of A. firma. This physiological characteristic seemed to be determined by seed sources rather than nodule sources.