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Enterotoxin Production and DNA Fingerprinting of Staphylococcus aureus Isolated from Diverse Samples by Pulsed-Field Gel Electrophoresis

  • Suh, Dong-Kyun
    • Biomedical Science Letters
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    • v.11 no.3
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    • pp.295-299
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    • 2005
  • Staphylococcus aureus is an important animal and human pathogen implicated in a variety of disease including food-poisoning caused by staphyloccal enterotoxins (SEs). In order to investigate the difference in genomic types and to monitor the transmission of S. aureus isolates, a total of 25 S. aureus isolates from different sources were determined for their genotypic characteristics by pulsed-field gel electrophoresis (PFGE) in addition to their ability to enterotoxin production and antibiotic resistance patterns in this study. All the isolates were susceptible to amikacin, and the resistance pattern to ampicillin and penicillin were most common among 14 different patterns. Eleven of 24 isolates produced one of three SEs, SEA, SEC or SED. Sixteen representative PFGE patterns were obtained by Smal restriction fragments of S. aureus isolates. Analysis of dendrogram based on PFGE band patterns suggested that food-poisoning outbreaks be caused by the diverse sources of food, of which their raw materials were infected with S. aureus. Also, it could be concluded that PFGE was a powerful tool for epidemiological tracing of infection source for food-initiated outbreaks.

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An Experimental Study of Fire Suppression Using a Water Mist in a Compartment (물분무를 이용한 화재제어에 관한 실험적 연구)

  • Kim, Sung-Chan;Park, Hyun-Tae;Ryou, Hong-Sun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.3
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    • pp.367-373
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    • 2003
  • The present study investigates the fire suppression characteristics using a water mist fire suppression system. The fire extinguishing times are measured for various fire sources, fuel types, and different total flooding rates of water mist. Pool fire with hydrocabon fuel is successfully extinguished within a minute under the operating conditions of the water mist system. Two different regimes of the smoke layer cooling are observed, such as rapid and slow cooling processes. The regimes are divided by threshold time which is calculated with auto-correlation function. The threshold time for the initial cooling decreases with increasing water flow-rates and fire sources. These initial cooling effects play an important role in preventing the occurance of flashover fire by the initial fire suppression.

Comparison of Radiosensitivity of Bacteria Isolated from Given Radiation Exposure History (방사선 피폭역을 달리하여 분리한 세균의 방사선감수성 비교)

  • Kim, Gi Su;Min, Bong Hui;Lee, Gang Sun
    • Korean Journal of Microbiology
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    • v.12 no.2
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    • pp.69-69
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    • 1974
  • This experiment was carried out to identify and to compare the radiosensitivities of bacteriz isolated from the sources of different radiation exposure histories. Among 10 strains isolated in this investigation, 4 strains of bacteria, Bacillus firmus, Bacillus brevis, Baciilus subtilis and Bacillus sphaericus were isolated from high and low radioactive sites simulaneously. Bacterial strains isolated from radioactive sources such as reactor and isotope production rooms were more resistant to irradiation than the microganisms from medical products and laboratories, however, there was no significance in radiosensitivity in the same species of bacteriz, even if they were isolated from different radiation exposure histories.

The Simulated study of different partial discharge sources in GIS (모의된 GIS에서 발생하는 부분방전에 관한 연구)

  • Kang, Dae-Yong;Wu, Guangning;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 1998.07e
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    • pp.1737-1739
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    • 1998
  • The paper presents experimental results of Partial Discharge(PD) in Gas Insulated Switchgear(GIS), obtained using a computer-aided PD measurement system. Different discharge sources are simulated and identified. These included internal, interface, surface and protrusion. For internal discharge, it showed symmetrical discharge type between positive and negative cycle. Interface discharge showed smaller number and higher peak than internal discharge but symmetrical discharge as well. For surface discharge, it showed small number and high peak in positive half cycle but large number and low peak in negative half cycle. For protrusion discharge, small number and high peak of discharge were shown only in negative half cycle.

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Heat source modeling of laser arc hybrid welding considering keyhole formation (키홀 형성을 고려한 레이저 아크 하이브리드 용접 열원 모델링)

  • Jo, Yeong-Tae;Na, Seok-Ju
    • Proceedings of the KWS Conference
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    • 2005.06a
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    • pp.97-99
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    • 2005
  • Laser arc hybrid process is actively researched as a new welding method since it has several advantages by the combination of laser beam and electric arc. By the coupling of two different heat sources, laser and arc mutually assist and influence. High power laser can make the deep keyhole and arc plasma can form the large bead shape. In this paper the effect of two different heat sources to weld bead are investigated and as a result of analysis, it is shown that the lower part of keyhole is heated by laser and the upper part of weld pool is dominantly heated by arc.

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The polymerization rate and the degree of conversion of composite resins by different light sources

  • Ryoo, Joo-Hee;Kwon, Hyuk-Choon
    • Proceedings of the KACD Conference
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    • 2003.11a
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    • pp.565-566
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    • 2003
  • The clinical performance of light polymerized composite resins is greatly influenced by the quality of the light curing unit used. Commonly used halogen light curing units(LCUs) have some specific drawbacks such as decreasing light output with time. To overcome this, the blue LED LCUs are newly developed and introduced. The purpose of this study was to observe the reaction kinetics and the degree of polymerization of composite resins when cured by different light sources and to evaluate the effectiveness of the blue LED LCUs compared with conventional halogen LCUs.(omitted)

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Use of stem cells in bone regeneration in cleft palate patients: review and recommendations

  • Amiri, Mohammad Amin;Lavaee, Fatemeh;Danesteh, Hossein
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.48 no.2
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    • pp.71-78
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    • 2022
  • This study was conducted to review the efficacy of different sources of stem cells in bone regeneration of cleft palate patients. The majority of previous studies focused on the transplantation of bone marrow mesenchymal stem cells. However, other sources of stem cells have also gained considerable attention, and dental stem cells have shown especially favorable outcomes. Additionally, approaches that apply the co-culture and co-transplantation of stem cells have shown promising results. The use of different types of stem cells, based on their accessibility and efficacy in bone regeneration, is a promising method in cleft palate bone regeneration. In this regard, dental stem cells may be an ideal choice due to their efficacy and accessibility. In conclusion, stem cells, despite the lengthy procedures required for culture and preparation, are a suitable alternative to conventional bone grafting techniques.

Examining Decision-Making of Participating in Open Innovation Platform: The Case of Biotechnology Industry

  • Son, Insoo;Lee, Jong-Ho;Lee, Dongwon
    • Asia Marketing Journal
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    • v.19 no.4
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    • pp.61-85
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    • 2018
  • Open innovation is based on a different knowledge landscape, with a different logic about the sources and uses of technologies. It implies that firms increasingly rely on external sources of innovation by emphasizing these ideas and resources. Using datasets from the UK biotechnology industry, this paper explores firms' willingness to participate in open innovation. The results indicate that the switching cost is identified as the direct predictor of the willingness to participate in open innovation. While high commitment based on the trust with partner increases switching costs, IT infrastructure within the firm decreases switching costs to open innovation. Taken together, this research broadens the study on open innovation by applying the switching cost as a medium of knowledge transfer in the biotech industry.

Locality Aware Multi-Sensor Data Fusion Model for Smart Environments (장소인식멀티센서스마트 환경을위한 데이터 퓨전 모델)

  • Nawaz, Waqas;Fahim, Muhammad;Lee, Sung-Young;Lee, Young-Koo
    • Proceedings of the Korea Information Processing Society Conference
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    • 2011.04a
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    • pp.78-80
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    • 2011
  • In the area of data fusion, dealing with heterogeneous data sources, numerous models have been proposed in last three decades to facilitate different application domains i.e. Department of Defense (DoD), monitoring of complex machinery, medical diagnosis and smart buildings. All of these models shared the theme of multiple levels processing to get more reliable and accurate information. In this paper, we consider five most widely acceptable fusion models (Intelligence Cycle, Joint Directors of Laboratories, Boyd control, Waterfall, Omnibus) applied to different areas for data fusion. When they are exposed to a real scenario, where large dataset from heterogeneous sources is utilize for object monitoring, then it may leads us to non-efficient and unreliable information for decision making. The proposed variation works better in terms of time and accuracy due to prior data diminution.

GREENHOUSE GAS EMISSIONS FROM ONSITE EQUIPMENT USAGE IN ROAD CONSTRUCTION

  • Byungil Kim;Hyounkyu Lee;Hyoungbae Park;Hyoungkwan Kim
    • International conference on construction engineering and project management
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    • 2011.02a
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    • pp.286-291
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    • 2011
  • Onsite usage of construction equipment accounts for a 6.8% of air pollution in Korea. The high concentration of carbon dioxide in such emissions impact not only climate change, but also people's health. However, greenhouse gas emissions from onsite equipment usage have not yet been fully investigated. This study presents a comparative analysis on how much greenhouse gas is generated by various equipment types used in different construction activities. Two ongoing cases which involve a typical road construction project in Korea were selected for the comparison purpose. Greenhouse gas emissions from each onsite equipment usage of the different activities were estimated on the ground of design documents. The estimates were compared and analyzed to derive the main sources of greenhouse gas emissions. The result showed that earthwork constituted the largest part-more than 90%-among work types. Dump truck, bulldozer, and loader were major sources for such emissions. The study results are expected to be used as a basis for reduction of greenhouse gas emission from onsite equipment usage.

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