• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.509-514
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• 2019

We investigated the beneficial effects of Ecklonia cava by-product (ECB), a residual product obtained after polyphenol extraction from Ecklonia cava, on normal rats. Male Sprague-Dawley rats were divided into the following three groups: Control group (NC), received basal diet; ECB 0.1 group, received basal diet supplemented with 0.1% ECB for 21 days; ECB 0.5 group, received basal diet supplemented with 0.5% ECB for 21 days. The productivity, serum immunoglobulin level, expression levels of muscle-related genes, and cecal microflora were measured in all the treatment groups to evaluate the potential use of ECB as a feed additive. The ECB 0.1 group exhibited enhanced expression of Myod, Myog, and Igf1 genes, which increased the body weight of rats. Additionally, treatment with ECB increased the cecal Lactobacillus spp. counts and the serum immunoglobulin G levels in rats. Thus, ECB can effectively increase body weight and can be a potential feed additive for improving growth.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.538-544
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• 1998

One hundred and twenty-eight finishing pigs (51.3 kg average initial body weight) were used to determine the effects of adding cellulase enzymes and lactobacillus acidophilus to sorghum-based diets on growth performance, carcass merit, and nutrient digestibility in finishing pigs. Treatments were: 1) corn-soybean meal-based positive control; 2) sorghum-soybean meal-based negative control; 3) Diet 2 with celluloytic enzymes; and 4) Diet 2 with a bacterial feed additive (lactobacillus acidophilus). There was a trend for greater average daily gain (ADG) in pigs fed com versus the sorghum treatments for day 0 to 28 (p < .09), but there was no effect of treatment (p > .15) on overall ADG (i.e., day 0 to 63). Feed consumption was not affected by treatment during the experiment (p > .19). Pigs fed the corn-soybean meal-based diet had 3.5% greater overall gain/feed than pigs fed the other diets (p < .009). Dressing percentage was not affected by treatment (p > .22), but there was a trend for backfat thickness at the last rib to be greater for pigs fed com versus the sorghum treatments (p < .09). Pigs fed the sorghum treatments had 1 % greater fat free lean index (p < .10) compared to pigs fed the corn-soybean meal-based positive control. Pigs fed com had greater apparent digestibilities of DM, N, and GE than pigs fed the sorghum treatments (p < .03), and greater DE intake (p < .07) suggesting that the increased carcass fatness for pigs fed the corn-based control diet resulted from greater energy status of those pigs. In conclusion, pigs fed the corn-soybean meal-based control diet had no improved growth performance but tended to be fatter than pigs fed sorghum. Adding cellulolytic enzymes or a bacterial feed additive to diets for finishing pigs did not affect growth performance, carcass merit, or nutrient utilization.

• Fisheries and Aquatic Sciences
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• v.23 no.4
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• pp.7.1-7.8
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• 2020

Background: To minimize the use of antibiotics and to obtain a more sustainable fish culture and aquaculture industry, development of alternative natural source of immunostimulant to replace antibiotic in aquafeed is highly needed. Objective: Dietary inclusion effect of yacon (YC), ginger (GG), and blueberry (BB) on growth, body composition, and disease resistance of black rockfish against Vibrio anguillarum was compared to ethoxyquin (EQ). Methods: Four hundred eighty juvenile (an initial weight of 4.2 g) fish were randomly distributed into 12 of 50 L flowthrough tanks (forty fish per tank). Four experimental diets were prepared; the control (Con) diet with 0.01% EQ inclusion, and YC, GG, and BB diets at 1% each additive inclusion. Each additive was included into the experimental diets at the expense of wheat flour. Each diet was assigned to triplicate tanks of fish and hand-fed to satiation twice daily for 8 weeks. At the end of 8-week feeding trial, 20 fish from each tank fish were artificially infected by intraperitoneal injection with 0.1 mL of culture suspension of pathogenic V. anguillarum containing 3.3 × 10⁶ cfu/mL respectively. Fish were monitored for the following 8 days after V. anguillarum infection and dead fish were removed every 6 h for the first 4 days and 12 h for the rest of the study. Results: Weight gain, specific growth rate (SGR), and feed efficiency ratio (FER) of fish fed the YC diet was higher than those of fish fed all other diets. However, feed consumption, protein efficiency ratio, and protein retention was not affected by dietary additive. Moisture, crude protein, and crude lipid content of the whole body of fish were affected by dietary additive. Analysis of the Kaplan-Meier survival curves showed that survival of fish fed the YC, BB, and GG diets was higher than the Con diet. Conclusion: Oral administration of YC can improve not only weight gain, SGR, and FER of black rockfish, but lower mortality of rockfish at occurrence of V. anguillarum.

• Animal Bioscience
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• v.34 no.3_spc
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• pp.405-416
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• 2021

Objective: This study evaluated the effects of feeding diets naturally contaminated with deoxynivalenol (supplemental 2 mg/kg) on health, growth, and the effects of a mycotoxin-detoxifying additive in newly-weaned pigs. Methods: Thirty-six pigs (27 day-old) were housed individually and assigned to 3 treatments for 5 weeks: CON (diet containing minimal deoxynivalenol), MT (diet with supplemental 1.9 mg/kg of deoxynivalenol), and MT+D (MT + mycotoxin-detoxifying additive, 0.2%, MegaFix, ICC, São Paulo, Brazil). The mycotoxin-detoxifying additive included bentonite, algae, enzymes, and yeast. Blood was taken at week 2 and 5. Jejunal tissue were taken at week 5. Data were analyzed using the MIXED procedure of SAS. Results: Pigs fed MT+D tended to have decreased (p = 0.056) averaged daily feed intake during week 1 than MT. At week 2, serum aspartate aminotransferase/alanine aminotransferase in MT tended to be lower (p = 0.059) than CON, whereas it was increased (p<0.05) for MT+D than MT, indicating hepatic damages in MT and recovery in MT+D. Pigs fed MT had lower (p<0.05) blood urea nitrogen/creatinine than CON, supporting hepatic damage. At week 5, pigs fed MT tended to have reduced (p = 0.079) glucose than CON, whereas it was increased (p<0.05) for MT+D than MT, indicating impaired intestinal glucose absorption in MT, which was improved in MT+D. Pigs fed CON tended to have increased (p = 0.057) total glutathione in jejunum than MT, indicating oxidative stress in MT. Pigs fed MT+D had a reduced (p<0.05) proportion of Ki-67-positive cells in jejunum than MT, indicating lower enterocyte proliferation in MT+D. Conclusion: Feeding supplemental 1.9 mg/kg of deoxynivalenol reduced growth and debilitated hepatic health of pigs, as seen in leakage of hepatic enzymes, impaired nitrogen metabolism, and increase in oxidative stress. The mycotoxin-detoxifying enhanced hepatic health and glucose levels, and attenuated gut damage in pigs fed deoxynivalenol contaminated diets.

• Nutrition Research and Practice
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• v.8 no.3
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• pp.233-240
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• 2014

Epidemiology studies indicate that diet or specific dietary components can reduce the risk for cancer, cardiovascular disease and diabetes. An underlying cause of these diseases is chronic inflammation. Dietary components that are beneficial against disease seem to have multiple mechanisms of action and many also have a common mechanism of reducing inflammation, often via the $NF{\kappa}B$ pathway. Thus, a plant based diet can contain many components that reduce inflammation and can reduce the risk for developing all three of these chronic diseases. We summarize dietary components that have been shown to reduce cancer risk and two studies that show that dietary walnut can reduce cancer growth and development. Part of the mechanism for the anticancer benefit of walnut was by suppressing the activation of $NF{\kappa}B$. In this brief review, we focus on reduction of cancer risk by dietary components and the relationship to suppression of inflammation. However, it should be remembered that most dietary components have multiple beneficial mechanisms of action that can be additive and that suppression of chronic inflammation should reduce the risk for all three chronic diseases.

• Journal of Aquaculture
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• v.20 no.1
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• pp.1-6
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• 2007

Two feeding experiments were conducted to investigate the effects of fermented cactus fruit (Opuntia ficusindica) fluid (FCFF) as a feed additive to a commercial diet on the growth of red sea bream, Pagrus major, and to determine an effective dose. FCFF was prepared by mixing crushed cactus fruit with a starch solution and commercially available microorganisms for 2 weeks at room temperature. Three triplicate groups of red sea bream had initial mean weights of 84.1 g (Exp-1) and 5.1 g (Exp-2) and were fed experimental diets containing 0%, 1%, and 5% FCFF for 2 months (Exp-1) and 0%, 0.2%, 0.5%, and 1% FCFF for 3 months (Exp-2), respectively. In experiment 1, the mean body weight of fish fed the diet containing 1% FCFF was significantly higher (P<0.05) than that of fish fed the diet without FCFF. The survival rate was highest in fish fed the diet containing 1% FCFF, although the difference was not statistically significant. The feed gain ratio (FGR), specific growth rate (SGR), and condition factor (CF) values of fish fed the diet containing 1% FCFF were higher than those of fish in the other dietary groups that received lower levels of FCFF. The daily feeding rate (DFR) of fish fed the diet containing 1% FCFF was slightly lower but not statistically different than the DFR values of fish in the other dietary groups. In experiment 2, the final mean body weight of fish fed the diet containing 1% FCFF was significantly (P<0.05) higher than the mean weight of fish in the control group. The FGR, SGR, and CF values of fish fed the diet containing 1% FCFF were better than the values from fish in the other dietary groups that received lower levels of FCFF, although the differences were not statistically significant. However, the DFR of fish fed the diet containing 1% FCFF was lower than those of fish in the other groups. These results suggest that FCFF could be used as a feed additive in commercial fish food and a preferable level of supplementation is at least 1.0% in fingerling and young red sea bream.

• Journal of Fisheries and Marine Sciences Education
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• v.25 no.6
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• pp.1285-1293
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• 2013

This study was conducted to investigate the effects of dietary inclusion of various additives Solid sulfur, fucoidan, and glucan on growth performance and immune responses in Juvenile olive flounder paralichthys olivaceus. Six experimental diets supplemented with : no additives (CON); 0.5 and 1.0% solid sulfur (S); 0.2 and 0.4% fucoidan (F); 0.1% glucan (G) of these additives diet on a dry-matter basis. Fish averaging $8.2{\pm}0.17g$ were fed one of six experimental diets in triplicate groups for 6 weeks. By the end of the feeding trial, weight gain, feed efficiency, specific growth rate, protein efficiency ratio, hepatosomatic index and condition factor of fish fed diet CON were significantly lower then those of fish fed the all additives diets (P < 0.05). In challenge test, fish were infected by intraperitonel injection of 0.1 ml bacterial suspension with Edwardsiella tarda per fish after the feeding trial. As a result fish fed All additive diet showed a lower cumulative mortality then did fish fed CON diet throughout the challenge test. In conclusion, these results indicated that solid sulfur, fucoidan and glucan enhanced the growth, feed efficiency and non-specific immune activity of juvenile flounder and protect the fish against microbial infections.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1274-1279
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• 2003

Thirty-six Naeimi ram lambs were equally and randomly allotted to four treatment groups with three replications per treatment to determine the simple and additive effects of monensin and zeranol on growth performance, carcass characteristics and nutrient digestibility. The treatment groups were: basal diet-fed lambs (C), monensin-fed lambs (M) where the basal diet was supplemented with 33 mg monensin per kilogram DM, lambs implanted with 12 mg zeranol (Z), and monensin-fed lambs implanted with zeranol (MZ). Lambs fed monensin-containing diet consumed 10.5% less (p<0.05) DM/100 kg weight and were 8.3% more (p<0.05) efficient in converting feed than lambs fed control diet. Zeranol implanted lambs tended to grow 35.2% (p<0.05) faster, consumed 5.1% more (p<0.05) feed and were (p<0.05) 21.9% more efficient in their feed conversion than control lambs. Responses of lambs to monensin and zeranol implants were not additive. Except for Z treatment, there were no marked differences in all carcass characteristics among the various treatment groups. Z-lambs produced 12.7% heavier (p<0.05) carcasses compared with those from C treatment. Also fat parameters, namely, kidney and pelvic fat (KP), body wall thickness and fat thickness, indicated trends for higher finish in Z treatment lambs (p<0.05) than for those lambs from other treatments. Except for CF and ADF, no significant differences in nutrients digestibility were noticed between various treatments; feeding monensin resulted in 24.5% and 8.5% depressions (p<0.05) in CF and ADF digestibility, respectively in comparison to C treatment. Nitrogen retention as percentage of total N-intakes was averaging 7.5 and 20.2% higher (p<0.05) in lambs implanted with zeranol than those fed the M and C diets, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.634-643
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• 2023

The effects of dietary supplementation with fermented blood meal (FBM) in two forms were evaluated on the growth performance, hematological parameters, innate immune response, antioxidant capacity, digestive enzyme activity and intestinal histology of Japanese eel Anguilla japonica. A commercial powder feed was used as a basal diet (Con). Six other diets were prepared by supplementing 2, 4 and 6% FBM either in powder or liquid form to the Con diet (designated as P2, P4, P6, L2, L4 and L6, respectively). In total 420 eels (initial mean body weight: 55.1±1.71 g) were distributed into 21 tanks (450L) at a density of 20 eels per tank. Three replicate groups of fish were fed one of the seven diets twice daily for 12 weeks. No significant differences were observed in the growth performance and survival among all groups. Fish fed FBM-containing diets showed significantly improved hematological parameters, innate immunity and antioxidant capacity than those in the Con group. Fish fed FBM-containing diets showed significantly enhanced digestive enzyme activity, villus length and goblet cells than those in fish fed Con diet. These results indicate that both powder and liquid FBM could be a good functional feed additive to improve innate immunity and digestion in Japanese eel.

• Nutritional Sciences
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• v.8 no.1
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• pp.3-9
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• 2005

Obesity can be defined as a metabolic disease due to an increased fat accumulation in the body caused by an imbalance of calorie intake and output The prevalence of obesity has increased substantially over the past 2-3 decades in developed and developing countries. The health impact of weight gain is so marked that obesity has now been classified as a major global public health problem In order to investigate the effect of diet conversion and oral administration of Platycodon grandiflorum extracts on the treatment of obesity, male Spraque-Dawley rats were divided into four groups: a group converted to normal diet (Control group), a group maintained high fat (30%) diet (H), and two groups with Platycodon grandiflorum extract added to the previously mentioned two groups. All animals were fed high fat diet for 7 weeks to induce the obesity. Then they were divided as mentioned above. Animals were fed experimental diet and Platycodon grandiflorum extract (150 mg/ml/rat/day) for 7 weeks. Body weight, adipose tissue weight (subcutaneous, epididymal, peritoneal fat pads) and serum lipids (total cholesterol and triglyceride) showed some differences among groups. The Platycodon grandiflorum feeding markedly decreased both body weight and adipose tissue weight in control group compared to H, high fat diet maintaining, group. Platycodon grandiflorum extracts significantly decreased the concentrations of serum lipids compared to H group. Fat cell numbers and sizes were significantly reduced in the oriental medicinal herb extract administrated group. Increased fatty acid binding protein (FABP) expression in high fat diet group was decreased by the dietary conversion to normal diet and the oral administration of Platycodon glandiflorum extracts. In contrast, there was no significant effect on FABP expression in the high fat maintenance group. In this study, the conversion from high fat diet to low fat or normal diet had a beneficial effect on body weight loss and serum lipid profiles. Dietary Platycodon glandiflorum extracts had an additive beneficial effect on the prevention and treatment of obesity.