• Proceedings of the KIEE Conference
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• 1995.11a
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• pp.597-599
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• 1995

We have analyzed the dielectrophoretic(DEP) force on a cell in a micro planar electrode structure. We fabricate a micro planar electrode structure using micro machining technology and measure the motion of a cell that is accelerated by DEP force. DEP force on a cell is calculated by curve fitting the motion of a cell. Radish and yeast are used for the experiment. In case of radish, DEP force is increased as the voltage and the frequency is increased, and in case of yeast, DEP force is increased only as the voltage is increased DEP force on a yeast does not vary when the frequency varies from 1 MHz to 3 MHz. The result will be helpful to the manipulation of cells using DEP force.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.147-148
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• 2013

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.50 no.3
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• pp.136-143
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• 2001

A micro particle manipulator, which is devised for trapping particles at fixed positions by negative dielectrophoretic force (DEP force), has been fabricated and experimented. It is composed of square type electrode arrays fabricated by nickel electroplating with the height of 28 ${\mu}m$. To improve the quality of electroplated nickel electrodes, plating conditions have been optimized. Micro particles used in this study are polystyrene spheres and their to the specific position and trapped. The DEP force along the moving path of the particles has been estimated by the motion equation of a single particle. The displacement of a particle with an elapsed time was measured using a high-speed camera (1000 frames/sec). The velocity and acceleration of the particle were calculated from the measured data. The DEP force acting on the particle was estimated.

• Journal of Biomedical Engineering Research
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• v.34 no.3
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• pp.123-128
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• 2013

The 'Dielectrophoretic Tweezers(DEP Tweezers)' can be used as a facile, economical toolkit for quantitative measurement of chemical and biological binding forces related to many biological interactions within a microfluidic device. Our experimental setup can probe the interaction between a single receptor molecule and its specific ligand. Immunoglobulin G(IgG) functionalized on polystyrene microspheres has been used to detect individual surface linked Staphylococcus protein A(SpA) molecules and to characterize the strength of the noncovalent IgG-SpA bond. It was measured and compared with the existing measurements. Measured single binding force of between Goat, Rabbit IgG and SpA were $17{\pm}7pN$, $74{\pm}16pN$. This work can be used to investigate several different ligand-receptor interactions and antigen-antibody interactions.

• Journal of Electrical Engineering and information Science
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• v.2 no.4
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• pp.66-71
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• 1997

The dielectrophoretic(DEP) force acting on a cell in an electric field is experimentally determined. A cell is accelerated by the DEP force in an electric field generated between micro planar electrodes. the position of the cell is measured and the velocity and acceleration of the cell are calculated based on the measured position data. The DE force is determined from the motion equation of a moving cell in suspension. The electrode structure is fabricated by micromachining technology and the height of electrodes is 1 $\mu\textrm{m}$. Radish cell and yeast are used in th experiments. In the case of radish cell, the DEP force increases as voltage or frequency(1MHz∼3MHz) increases. The voltage dependence can be explained that the DEP force increases when ▽│E│$^2$increases. The frequency dependence means that Re[x\ulcorner] of radish cell is maximized in a certain frequency. In the case of yeast, the DEP force increases only as voltage increases. The reason for the voltage dependence is the same with the case of radish. The DEP force increases only as voltage increases. The reason for the voltage dependence is the same with the case of radish. The DEP force on a yeast does not vary when the frequency varies from 1MHz to 3MHz. This result coincides with the fact that the value of calculated Re[x\ulcorner] is constant in the test frequency range.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.59 no.2
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• pp.385-389
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• 2010

This paper presents a microfabricated nanowire diluter which dilutes the concentration of nanowires in solution instead of by the conventional centrifuge process. The device has 16 pairs of gold electrodes in a micro channel composed of a glass substrate and PDMS. We prepared nickel nanowires by the template-directed electrodeposition method using nanoporous anodized aluminum template (AAO). We injected the Dimethylformamide (DMF) solution containing nanowires into the inlet of the diluter while applying square wave voltages on the electrodes to trap the nanowires at the subsequent gold electrodes by means of dielectrophoretic attraction forces. The concentration of nanowires at the outlet of the micro channel was changed as we expected, which illustrates that the device can effectively dilute nanowires and can be applied to a controlled assembly of nanowires.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.11
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• pp.824-831
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• 2008

We present a continuous size-dependent particle separator using a negative dielectrophoretic (DEP) virtual pillar array. Two major problems in the previous size-dependent particle separators include the particle clogging in the mechanical sieving structures and the fixed range of separable particle sizes. The present particle separator uses the virtual pillar array generated by negative DEP force instead of the mechanical pillar array, thus eliminating the clogging problems. It is also possible to adjust the size of separable particles since the size of virtual pillars is a function of a particle diameter and applied voltage. At an applied voltage of 500 kHz $10\;V_{rms}$ (root mean sqaure voltage) sinusidal wave and a flow rate of $0.40\;{\mu}l\;min^{-1}$, we separate $5.7\;{\mu}m$-, $8.0\;{\mu}m$-, $10.5\;{\mu}m$-, and $11.9\;{\mu}m$-diameter polystyrene (PS) beads with separation purity of 95%, 92%, 50%, and 63%, respectively. The $10.5\;{\mu}m$- and $11.9\;{\mu}m$-diameter PS beads have relatively low separation purity of 50% and 63%. However, at an applied voltage of $8\;V_{rms}$, we separate $11.9\;{\mu}m$-diameter PS beads with separation purity over 99%. Therefore, the present particle separator achieves clog-free size-dependent particle separation, which is capable of size tuning of separable particles.

• Proceedings of the KIEE Conference
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• 1993.11a
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• pp.201-203
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• 1993

Recently, micro-scaled cell fusion system in the bioiogical cell fusion is preferred to Nacro-scaled one by dint of microelectronic processes. The microfabricated cell fusion system has its components such as fusion chamber, selector and detector. In this paper, we describe the design rules of the micro-fabricated cell fusion system using dielectrophoretic force and analyze its components using finite element method.

• Proceedings of the KIEE Conference
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• 1994.07a
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• pp.183-185
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• 1994

A new method for determination of electric parameter of cell membranes is proposed. Two circular electrodes is designed to have repulsive force. From the potential energy analysis, stable points where a cell is levitated between electrodes exist and move as frequency or voltage change. The levitated cell in the stable point fall freely when DEP force is zero. The DEP force is dependent on the frequency and the force is zero at the critical frequency. The critical frequency is determined by measuring the difference between the time taken at zero-applied voltage and the time taken at the frequency and the voltage. For example, the critical frequency and stable points of N.crassa slime cell is numerically evaluated. In the exeriment, polystyrene in water is levitated at the stable point. We show that the stable point move as the applied voltage is changed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.04a
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• pp.57-58
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• 2009

The dynamics of nano or micro sized-particles in liquid crystal (LC) medium under an external electric field is of theoretical and technological interest. A fullerene of 10 wt% was doped into the LC medium and its electric field induced motion was controlled by both in-plane and vertical electric fields. In the proposed device, pixel electrode I and pixel electrode II were designed consecutively on the bottom substrate and common electrode on the top of the substrate. When the electric field was applied, the fullerenes start to move in direction of applied electric field. The dark, grey and white states in the proposed device can be obtained by suitable combination of the polarity of applied electric field at pixel electrode I, pixel electrode II and common electrode. The dynamical motions of fullerene particles in LC medium suggest that fullerene can be designed for electronic-paper like displays.