• Journal of Photoscience
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• 제12권3호
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• pp.163-169
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• 2005

Recent reports suggest that floral organs such as sepals, petals, stamens, and carpels are specified by quaternary MADS protein complexes with different combinations. The formation of quaternary complexes of ABCDE MADS proteins may be the molecular basis of ABCDE model for the floral organ development. The MADS complexes involved in each floral organ development seem to be conserved in at least dicot species although detailed molecular mechanism is slightly different depending on species. Even in monocot, at least rice, MADS complexes similar to those in dicot exist, suggesting that the floral organ specification by MADS protein complexes may be conserved in most of plants. The MADS protein complexes may have more specific recognition of target genes or more transcription activation ability than monomers or dimers, resulting in finely regulated floral organ development.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.171-178
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• 2002

Chlorophyll (Chl) fluorescence imaging was used to investigate the effectiveness of in vivo incorporation methods for two chemicals, 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) and methyl viologen (MV) in rice, a monocot, and cucumber, a dicot, leaves. four different methods (vacuum infiltration, floating, transpiration-aided incorporation through petiole and spraying) were compared, and $F_i$ and $F_v$/$F_m$ were chosen for the imaging of the DCMU- and MV-treated leaves, respectively. The effects of the chemicals in plants were generally heterogeneous over the whole leaf area. Moreover, the effectiveness of the treatment of a chemical in plant leaves was dependent on chemical species, plant species, concentration of the chemical, the treatment method, the duration of the treatment, the existence of light and detergent, etc. In conclusion, we suggest that to achieve the proposed effects of chemicals in plants for an actual experiment, these factors must be considered before the chemical treatment, and the best method for the treatment of the chemicals tested was floating and vacuum infiltration in the dicot and the monocot leaves, respectively, as drawn from Chl fluorescence imaging analysis.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
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• pp.67-67
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• 2002

• 환경위생공학
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• 제5권2호
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• pp.89-99
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• 1990

Morphology, anatomy and diurnal acid fluctuations were investigated monthly for the succulent C4 dicot Portulaca grandiflora Hook. growing under natural environmental conditions. Modified Kranz-Type sturcture and typical CAM-like cells were shown in leaves and stems, respectively. Values of indices of mesophyll succulence inleaves stems were 5.62 and 16.68, respectively. The number of stomata were 1476$\textrm{cm}^{-2}$ in leaves while the stomata in stems were not observed through growing seasons from spring to summer. However, on Sep. 16, 1981 in defoliate autumn season, stomata were begun to be produced for the first time in stems and the number were increased gradually to 216$\textrm{cm}^{-2}$ on Oct. 29. This feature can be interpreted as a seasonal plant dimorphism. P. grandiflora exhibited a diurnal fluctuation of titratable acidity in leaves and stems. Seasonal amplitudes of acid fluctuation in stems were as follows: 27 $\mu$eq./g.f.wt. on Sep. 21; 57$\mu$eq/g.f.wt. on Oct. 3; 80$\mu$eq./g.f.wt. on Oct. 21. Such the results in seasonal amplitude were able to consider due to decrease of light period and cool night air temperature according to change of season. Also, the naturally defoliated stems which had already stomata exhibited usual diurnal acid fluctuation, on the other hand the treated stems which possessed artificially closed stomata showed a few of acid fluctuation. Especially, stomata in stems are developed by seasonal dimorphism and activated stomata participate in CAM behavior of stems.

• Molecules and Cells
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• 제40권11호
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• pp.828-836
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• 2017

Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.115-121
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• 2000

A cDNA clone encoding chloroplast triosephosphate isomerase (TPI-cp) was isolated from strawberry fruit cDNA library. Sequence analyses indicated that the cDNA contains an open reading frame of 314 amino acids (33.5 kDa) composed of a transit peptide (59 amino acids) in amino terminal region and mature protein (255 amino acids). The existence of transit peptide in the deduced amino acid sequence implies that it encodes a chloroplast isoform. The protein sequence is more similar to other plant chloroplast isoforms than cytosolic isoforms. RNA blot analysis indicated that its expression is ubiquitous in examined five tissues, flowers, leaves, petioles, roots and fruits, and shows differential pattern according to fruit ripening. Genomic DNA blot analysis showed that TPI-cp is encoded by multiple genes in strawberry. Through sequence comparison and phylogenetic tree construction, TPI-cp is distinctively grouped into dicot and chloroplast isoforms.

• Journal of Plant Biotechnology
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• 제5권3호
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• pp.149-155
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• 2003

Metabolic engineering for production of isoflavones in nonlegume plants could distribute the health benefits of these phytoestrogens in more widely-consumed grains. Series of investigation to check the ability of the heterologous isoflavone synthase enzyme to interact with the endogenous phenylpropanoid pathway have been conducted. Overall, results provide possibility of production of isoflavonoids in several plant tissue systems including soybean and nonlegumes. In tissue that undergoes naturally enhanced synthesis of anthocyanins, genistein production was enhanced. In a monocot cell system, introduced expression of a transcription factor regulating genes of the anthocyanin pathway was effective in conferring the ability to produce genistein in the presence of the isoflavone synthase gene. However, in this case the intermediate accumulated to high levels indicating an inefficiency in its conversion. Introduction of a third gene, chalcone reductase, provided the ability to synthesize an additional substrate of isoflavone synthase resulting in production of the isoflavone daidzein. These research efforts provide insight into requirements for metabolic engineering for isoflavone production in nonlegume dicot and monocot tissues.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.77-84
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• 2003

Metabolic engineering for production of isoflavones in non-legume plants could distribute the health benefits of these phytoe-strogens in more widely-consumed grains. We investigate the ability of the heterologous isoflavone synthase enzyme to interact with the endogenous phenylpropanoid pathway. Overall, results provide possibility of production of isoflavonoids in several plant tissue systems including soybean and non-legumes. In tissue that undergoes naturally enhanced synthesis of anthocyanins, genistein production was enhanced. In a monocot cell system, introduced expression of a transcription factor regulating genes of the antho-cyanin pathway was effective in conferring the ability produce genistein in the presence of the isoflavone synthase gene. However, in this case the intermediate accumulated to high levels indicating an inefficiency in its conversion. Introduction of a third gene, chalcone reductase, provided the ability to synthesize an additional substrate of isoflavone synthase resulting in production of the isoflavone daidzein. These research efforts provide insight into requirements for metabolic engineering for isoflavone production in non-legume dicot and monocot tissues.

• Journal of Plant Biology
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• 제39권1호
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• pp.9-13
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• 1996

A clone, LCM1, which encodes calmodulin (CaM) was isolated and characterized from monocot lily (Lilium longiflorum Thunb.) plants. The clone is 681 bps and contains the 447 bp coding region, 8 bp leader sequence, 210 bp 3'-untraslated region, and a poly(A) tail. The coding region of 149 amino acids encodes a protein of predicted Mr 17 kD. Comparison of the LCM1 amino acid sequence with other CaMs revealed that the protein is highly conserved among various living organisms. The expression level of calmodulin gene in lily was studied by RNA blot analysis. The LCM1 mRNA was present in all tissues tested. However, a higher level of calmodulin was observed in anther and floral bud. The level of calmodulin mRNA in anther was about 10 times higher than that in anther was about 10 times higher than that in vegetative tissues. The anther preferential expression of CaM in lily is currently investigated in dicot plants.

• 한국잡초학회지
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• 제17권3호
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• pp.256-261
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• 1997

배 과수원에 발생하는 잡초종을 잡초생장기인 6월 중순에 조사한 결과 광엽초종은 쑥, 명아주, 여뀌, 망초, 닭의장풀, 메꽃, 비름 등이 우점발생하였고 화본과초종은 피, 바랭이, 강아지풀 등이 발생빈도가 높은 경향으로 나타나 우리나라 과수원에 발생하는 대표적인 잡초종들이 거의 분포하였다. 새로운 경엽처리 제초제인 carfentrazone-ethyl의 단제와 glyphosate 및 glufosinate의 약량에 따른 혼합처리시 과수에 대한 약해증상은 나타나지 않았으며 carfentrazone-ethyl의 단제처리구에서는 광엽초종에 대한 살초효과는 뚜렷하였으나 화본과 및 사초과초종에 대한 효과는 미약하였다. Glyphosate나 glufosinate의 단제 처리구에서 보다 carfentrazone-ethyl과의 혼합처리구에서 glyphosate나 glufosinate를 표준약량의 절반으로 혼합하더라도 약효발현이 빠르게 나타났으며 혼합처리에 의해 화본과 및 사초과초종에 대한 살초효과가 상승하는 경향이었다. 처리후 10일까지 carfentrazone-ethyl은 glyphosate와의 혼합처리구에서보다 glufosinate와의 혼합처리구에서 광엽 및 화본과초종에 대한 살초효과가 높았으나 시간이 경과할수록 glyphosate와의 혼합처리구에서 약효의 지속기간이 길어점에 따라 후발초종에 대한 방제효과도 높은 것으로 판단되어 carfentrazone-ethyl은 glyphosate와 혼합처리시 과수원에 발생하는 잡초종을 효과적으로 방제할 수 있을 것으로 생각되었다.