• 한국균학회지
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• 제39권3호
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• pp.227-228
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• 2011

한국 식품연구원에서 분양받은 48종의 효모들의 PD broth 배양 농축물들의 혈전용해활성을 조사하였다. Saccharomyces cerevisiae Y99-7의 PD broth 배양 농축물이 25 mm의 용해환(clear zone)을 보여 가장 높은 혈전용해 활성을 보였다. 또한 선발 효모를 PD broth 배지로 $30^{\circ}C$에서 24 h 배양하였을 때 yeast extract-peptone dextrose 배양농축물 보다 높은 혈전용해활성을 보였다.

• 한국의류학회지
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• 제32권2호
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• pp.319-327
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• 2008

The purpose of this study is to propose the development of high quality electromagnetic wave shielding fabrics. Silver nitrate is used for polyester fabric as an electromagnetic wave shielding material. The effects of activators and electroless silver plating condition on the evenness and adhesion of silver to fabrics, are observed through the SEM micrographs. Surface morphology and wash-ability are measured using SEM. The results are as follows: The optimum weight loss by alkaline hydrolysis of polyester fabrics is about 20%. The optimum concentration of $SnCl_2$ and $PdCl_2$in catalyst reaction using $PdCl_2$ as an activator is 2.5g/L and 0.5g/L, respectively. The optimum concentration of dextrose to improve adhesion between the silver plating and fabrics is 45g/L. The optimum concentration of silver nitrate in the catalyst reaction, using $PdCl_2$ as an activator is 56g/L, respectively. The optimum plating temperature and time are $15^{\circ}C$ and 30minutes, respectively.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제48권1호
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• pp.33-40
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• 2022

Objectives: Prolotherapy is a method that has gained popularity in recent years and has been reported to have positive short-term and long-term clinical results in maxillofacial surgery, especially temporomandibular joint (TMJ) hypermobility. This study aimed to evaluate the changes in the trabecular structure of mandibular condyles in patients who underwent prolotherapy due to TMJ hypermobility using the fractal analysis method. Materials and Methods: Forty-five patients who received dextrose prolotherapy at a concentration of 20% and fifteen control patients were included in the study. All patients had panoramic radiographs just before (T0) and six months after treatment (T1). The patients who received treatment were divided into three groups according to the number of prolotherapy injections. The regions of interest were selected from bone areas close to the articular surfaces of the condyles. The fractal dimension (FD) values were calculated. Results: The main effect of time on the FD value was significant [F (1, 56)=86.176, P<0.001]. This effect was qualified by a significant time×group interaction effect [F (3, 56)=9.023, P<0.001]. The decreases in FD values in all treatment groups between T0 and T1 times were significant (P=0.004). However, changes in FD values were not significant in the control group (P=0.728). Conclusion: Dextrose prolotherapy without the effect of the number of injections caused a decrease in FD values in the mandibular condyles over time.

• 생명과학회지
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• 제9권2호
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• pp.153-159
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• 1999

S. cerevisiae의 대표적인 구성적 promoter로 GAPDH, ADH1 및 ENO1를 사용하고 이들 promoter 하류에 INUl의 ORF를 in frame으로 연결한 각각의 plasmid pYIGP, pADHl-lNU 및 pENO-INU를 구축하였다. 이들 plasmid 를 함유한 재조합 S. cerevisiae SEY2102 균주들을 sucrose 함유 평판배지에서 선별한 후, 초기 포도당 농도가 2$\%$ 또는 4$\%$인 배지에서 배양했을 때, 모든 균주들은 12시간 이후부터 정지기에 들어갔으며, 정지기에서도 느리지만 균체증식과 inulinase 발현은 계속되었다. 4% 포도당 배지에서 inulinase 총발현량은 ADH1 promoter 계를 제외하고 GAPDH와 ENO1 promoter의 경우 2$\%$ 포도당 배지 때 보다 약 2배 증가한 2.0 unit/mL과 1.4 unit/mL를 각각 보였다. 단위균체농도당 inulinase 활성 즉, 비활성은 GAPDH와 ENOl promoter계의 경우 포도당 농도가 4$\%$일 때 2$\%$때보다 약 63$\%$ 정도의 비활성 증가를 나타내었다. 하지만 ADH1 promoter의 경우는 오히려 비활성이 약 40$\%$ 감소하였다. 그러나, plasmid 안정성 측면에서는 ADH1과 ENO1 promoter 발현계가 GAPDH promoter 경우의 34$\%$보다 훨씬 뛰어난 80$\%$이상을 보였다. 결론적으로 높은 포도당 농도에서 구성적 promoter의 활성 (발현능)은 GAPDH, ENO1, ADH1 promoter 순으로 나타났지만, 초기 포도당 농도가 높을 때나 에탄을 생산이 심각한 유가식 배양에서는 ENO1 promoter가 inulinase의 구성적 발현ㆍ생산에 더 적합할 것으로 사료된다.

• 대한수의학회지
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• 제15권2호
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• pp.207-213
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• 1975

Skimmed goat milk heated at $92^{\circ}C$ for 10 minutes was used as a basal extender for bull semen. The extenders for liquid semen were prepared by adding simultaneously at various ratio of 5% dextrose solution and egg yolk to skimmed goat milk. After bull seven was diluted with the extenders at the rate of 20 million spermatozoa per ml of the extenders. The extenders were stored at $5^{\circ}C$ and the survival rates of spermatozoa were examined at 4 and 24 hours, and 3, 5 and 7 days after dilution. The extenders for frozen semen were prepared by adding various ratlo of glycerol to skimmed goat milk containing 20 parts of 5% dextrose solution and 3 parts of egg folk to 77 parts of skimmed goat milk. After bull semen was diluted with the extenders at the rate of 40 million spermatozoa per ml of the extenders, the extenders were frozen in liquid nitrogen tank. The frozen extenders were thawed at $40^{\circ}C$ for 2 minutes, and the revival rates of the spermatozoa in the extenders were examined. These thawed extenders were stored at $5^{\circ}C$ and the survival rates of the spermatozoa were examined at 10 minutes and 24 hours and 3 and 5 days after thawing. The results obtained were as follows: 1. Among the extenders stored at $5^{\circ}C$, the survival rate of the sperm was the highest in the extender including 20 parts of 5% dextrose solution and 3 parts of egg yolk to 77 parts of skimmed goat milk, and the survival rate was significantly higher that of the spermatozoa in egg folk-2.9% sodium citrate (1 : 4) extender. (P<0.05) 2.Among the extenders frozen in liquid nitrogen tank, the revival rate of the spermatozoa was the highest in the extender containing 7ml of glycerol per 100ml of the extender with consisted of 77 parts of skimmed goat milk, 20hparts of 5% dextrose solution and 3 parts of egg yolk, and the revival rate was significantly higher than that of the spermatozoa in egg yolk-2.9% sodium citrate (1 : 4) extender containing 8ml of glycerol per 100ml of the extender (p<0.01). 3. Among the extenders stored at $5^{\circ}C$ after thawing, the survival rate of the spermatozoa was the highest in the extender containing 7ml of glycerol per 100ml of extender which consisted of 77 parts of skimmed goat milk, 20 parts of 5% dextrose solution and 3 parts of egg yolk, and the survival rate was significantly higher than that of the spermatozoa in egg yolk -2.9% sodium citrate (1 : 4) extender containing 8ml of glycerol per 100ml of the extender (p<0.01).

• 한국유기농업학회지
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• 제5권1호
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• pp.87-99
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• 1996

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP134 against pathogens causing major airborne diseases to apple tree, effect of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~$35^{\circ}$C, and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharides)>sucrose(disaccharides)$\geq$saccharose(di-saccharides)>starch (polysaccharides). Among different sugars, bacterial growth was favored by in the order of brown, black and white sugars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inoganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.171-174
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• 2005

20 g/L peptone, 20 g/L dextrose, 10 g/L yeast extract에 100 mg/L zeocin을 첨가하여 동일하게 전배양 한 재조합 Pichia pastoris X-33/pBPT44를 각기 다른 탄소원이 든 배지에 배양하면서 12시간 간격으로 샘플을 채취하여 배양시간에 따른 세포성장, pH, 각 탄소원에 따른 PLC 생산량 등을 측정하였다.

• Mycobiology
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• 제38권3호
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• pp.189-194
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• 2010

This study investigated the cultural characteristics of Shimizuomyces paradoxus in different nutritional and environmental conditions. The highest mycelial growth was observed in Schizophyllum (mushroom) genetics complete medium plus yeast extract agar medium, and the optimal temperature and pH were $25^{\circ}C$ and pH 8.0, respectively. The optimal carbon and nitrogen sources were 1% dextrose and 1% peptone in agar. However, in liquid culture the highest dry mycelium weight was found for the potato dextrose agar and potato sucrose agar broths. The optimum inoculum size was five mycelial discs (5 mm) per 100 mL of broth, and the optimum liquid culture period was 25 days. This is the first ever report of S. paradoxus cultural characteristics.

• 생약학회지
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• 제11권2호
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• pp.69-74
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• 1980

The rhizome of Polygonatum robustum Nakai (Liliaceae) has been used as a crude drug for the purpose of tonic and thirst cure in Korea. The dried rhizomes were extracted with hot ether, then the residue was extracted with hot alcohol. 1) The three kinds of chemical constituents, substance, I, II and III were isolated by silicagel column chromatography from ether and alcohol extracts of the rhizomes. Substance I was confirmed as ${\beta}-sitosterol$, $C_{29}H_{50}O$ by mass and IR spectroscopy. Substance II was identified as stigmasterol by GLC. 2) Substance III was obtained by crystallization from the column chromatography of alcohol fraction. It was suggested as diosgenin based on chemical and spectral discussions. 3) The concentration of blood sugar was significantly decreased in the group administered the ether extract with 20% dextrose and adrenaline in comparison to that of 20% dextrose and adrenaline along.

• Journal of Plant Biology
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• 제12권2호
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• pp.1-6
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• 1969

Effects of the special media on the mycelium growth in Agaricus campestris were studied. The results might be summarized as follows: 1. The mycelium growth fo Agaricus campestris were scarecely stimulated on the Peptone basal medium which was added 0.5gr. of Peptone and Dextrose basal medium which was added 1.5gr. of dextrose during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts showed a considerable growth for 144 hours. The order is as follows; Carrot-basal medium(4ml./100ml.)>Tomato-basal medium(2ml./100ml.)>Spinach-basal medium(3ml./100ml.). However, the spinach-basal medium among these three media were no significant difference.