한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of various carbon sources on the production of recombinant phospholipase C (PLC) by Pichia pastoris

  • Kim, Sun-Yong (Faculty of Applied Chemical Engineering, Bioprocess Technology lab, Chonnam National University) ;
  • Han, Kyung-Ah (Department of Material and Biochemical Engineering , Bioprocess Technology lab, Chonnam National University) ;
  • Rhee, Jong-Il (Faculty of Applied Chemical Engineering, Bioprocess Technology lab, Chonnam National University)
  • Published : 2005.04.15
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Abstract

The Pichia pastoris expression system is widely used for e production of recombinant proteins. In this study, we investigated the characteristics of cell growth and PLC production of recombinant Pichia pastoris. Especially, severed carbon sources like glycerol, glucose, sucrose, mannose, dextrose, xylitol, lactic acid, and acetic acid etc. were used to fermentations. The PLC activity was measured photometically.

20 g/L peptone, 20 g/L dextrose, 10 g/L yeast extract에 100 mg/L zeocin을 첨가하여 동일하게 전배양 한 재조합 Pichia pastoris X-33/pBPT44를 각기 다른 탄소원이 든 배지에 배양하면서 12시간 간격으로 샘플을 채취하여 배양시간에 따른 세포성장, pH, 각 탄소원에 따른 PLC 생산량 등을 측정하였다.

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