• Child Health Nursing Research
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• 제13권1호
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• pp.51-57
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• 2007

Purpose: The purpose of this study was to look for physical growth and developmental retardation of the disabled children in registered educational institutions. Method: The participants in this study were 103 children. The data was collected from January to September 2006. Body weight, height and head circumference were measured and the Denver II developmental screening tool was used to measure personal-social development. Results: For 40.8% of the children body weight was less than the average for Korean children of the same age, for 49.5% height was lower than average and for 44.7% head circumference was lower. For 84.5% of the children there was a delay in personal-social development, for 87.4% a delay in fine motor-adaptive development, for 80.6% a delay in speech development and for 81.6% a delay in gross motor development. Conclusion: Based on the findings of this study, it is necessary to develop an individual developmental programs for these children based on their growth and development status.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.171-171
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• 2002

This study was carried out by an Korean GLP laboratory to assess the combined repeated dose, reproduction and developmental toxicity of benzoyl peroxide for OECD SIDS(Screening Information Data Set) program. Male and female Sprague Dawley rats were exposed to benzoyl peroxide at levels of 0, 250, 500 and 1,000 mg/kg/day for 29 days for male and for 41-51 days for female.(omitted)

• Clinical and Experimental Pediatrics
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• 제61권4호
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• pp.114-120
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• 2018

Purpose: Routine screening for toxoplasmosis, rubella, cytomegalovirus (CMV), and herpes simplex virus (TORCH) in intrauterine growth restriction (IUGR) and small for gestational age (SGA) neonates has become a common practice. However, the incidence of TORCH varies across countries, and the cost of TORCH testing may be disadvantageous compared to disease-specific screening. To evaluate the efficacy of TORCH screening, the medical charts of IUGR or SGA neonates born in a single institution in Bucheon, Korea from 2011 to 2015 were reviewed. Methods: The clinical data of the 126 IUGR or SGA neonates were gathered, including gestational age, Apgar scores, neonatal sonographic findings, chromosome study, morbidities, developmental follow-up, and growth catch-up. Maternal factors including underlying maternal disease and fetal sonography were collected, and placental findings were recorded when available. TORCH screening was done using serum IgM, CMV urine culture, quantification of CMV DNA with real-time polymerase chain reaction, and rapid plasma reagin qualitative test for syphilis. Tests were repeated only for those with positive results. Results: Of the 119 TORCH screenings, only one was positive for toxoplasmosis IgM. This result was deemed false positive due to negative IgM on repeated testing and the absence of clinical symptoms. Conclusion: Considering the incidence and risk of TORCH in Korea, the financial burden of TORCH screening, and the single positive TORCH finding in our study, we suggest disease-specific screening based on maternal history and the clinical symptoms of the neonate. Regarding CMV, which may present asymptomatically, universal screening may be appropriate upon cost-benefit analysis.

• Toxicological Research
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• 제19권2호
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• pp.123-131
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• 2003

This study was carried out to assess the combined repeated dose, reproduction and developmental toxicities of benzoyl peroxide for OECD SIDS (Screening Information Data Set) program. Male and female Sprague-Dawley rats were exposed to benzoyl peroxide at dose levels of 0, 250, 500 and 1,000 mg/kg/day for 29 days for males and for 41-51 days for females. No deaths were found in all animals including control group during exposure period. No hematological effects attributable to benzoyl peroxide were observed in all treated groups. Significant decrease in the weight of testes and epididymis were observed in males at 1,000 mg/kg/day. In females at 1,000 mg/kg/day, slight histopathological effects in uterus such as epithelial vacuolation or hyperplasia were observed. No treatment-related changes in precoital time and rate of copulation, fertility and gestation period were noted in all treated groups. There was no evidence of teratogenic effect of benzoyl peroxide, but body weight of pups at 1,000 mg/kg/day was significantly decreased. NOAEL for combined repeated dose and reproduction/developmental toxicity was 500 mg/kg/day.

• Child Health Nursing Research
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• 제12권2호
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• pp.260-267
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• 2006

Purpose: This pilot study was to develop Korean version of CDI(The Child Development Inventory) and assess the validation and reliability on K-CDI. Method: The K-CDI is assessed by parent-report method that has designed to screen children developmental functioning on 12 months to 6.5 years of age /or older children who are judged to be functioning in the one to six-year range. The inventory assesses areas of child development in the social, self-help, motor, language, letter and number skills. also, it includes various symptoms and behavior problems that young children may have. Participants were 130 children and their mothers who has lived in Seoul or Gyounggi-Do. Data was analyzed on the ratio of item response, Pearson's correlation, and Cronbach alpha. Results: 270 items with 8 sub-developmental realms were confirmed the reliabilities and validity on Korean children. Total 300 items were selected for restandardization. There was a significant correlation between the score of Social Maturity Scale and K-CDI. The corrlation coefficient alpha was .98. Conclusion: This study indicated that CDI is applicable in the clinical and early childhood educational setting for developmental assessment in Korea.

• 한국수정란이식학회지
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• 제13권2호
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• pp.97-106
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• 1998

The pronuclear injection of metallothionein-human growth hormone (MT-hGH) gene into rabbit zygotes was performed to establish in vitro developmental system and to detect the presence of the injected gene by nested PCR. Mature female New Zealand White rabbits were superovulated by eGG and hCG treatments. The rabbits were mated and the zygotes were collected from the oviducts 18-22 h after hCG injection by flushing with D-PBS. Two to three picoliters of MT-hGH gene was microinjected into male pronuclei. The foreign gene-injected zygotes were cultured in TCM-199 or RD mediurn containing 10% FCS with a monolayer of rabbit oviductal epithelial cefls in a 5% $CO_2$ incubator. The presence of injected DNA in rabbit embryos or blastomeres at different developmental stages .vas detected by a nested PCR analysis. The results are summarized as follows ; 1.The developmental rate of the MT-hGH gene-injected zygotes to blastocyst was significantly higher in TCM-199 medium (68.1%) than in RD medium (42.9%). 2.The gene injection into pronuclei at 18 or 22 hours post hCG treatment during pronuclear stage did not much affect on the in vitro development of the rabbit embryos. 3.The rate of gene-positive embryos detected by the nested PCR analysis was significantly decreased when they developed to blastocysts. The results indicate that the screening of transgene in rabbit embryos by nested PCR analysis could be a prornisible method for the preselection of transgenic embryos. Furthermore, the preselection of transgenic embryos would greatly reduce hoth the cost and effort of production of transgenic animals.

• 한국산학기술학회논문지
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• 제11권9호
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• pp.3390-3397
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• 2010

본 연구의 목적은 지역사회 아동을 대상으로 발달지연 선별검사 중 부모-보고형 검사인 Korean-Ages and Stages Questionnaires(K-ASQ)의 타당도와 효용성을 알아보고자 하였다. 연구방법에서 연구대상은 선별검사에 동의한 229명 아동의 부모들과 유전적 질환 혹은 선천적 발달 지연이 보고되지 않은 3~5세 아동들이었다. 연구결과 K-ASQ는 민감도 0.41%, 특이도 0.76%, 과다의뢰율 0.14%, 과소의뢰율 0.21%이였으며, 지연발달과 정상발달 예측율은 지연 발달에서는 K-ASQ 0.70%, 정상발달 예측율은 K-ASQ 0.77%로 나타났다. K-ASQ의 타당도는 정상아동을 정상으로 선별하는 특이도가 민감도보다 높았으며, 지연발달과 정상발달 예측율에서도 K-ASQ는 70% 이상으로 임상에서 아동발달 선별에 유용하게 사용될 수 있을 것이다.

• 지역사회간호학회지
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• 제22권2호
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• pp.212-222
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• 2011

Purpose: This study was conducted to survey children's health status and need of customized visiting health care services in one province. Methods: The participants in this study were 237 caregivers of infants and preschoolers. Data were collected at the participant's home or public health center. Results: Many of the children did not receive developmental screening tests or dental examinations. In the beneficiary group, the prenatal checkup rate and children's vaccination rate were lower, and caregivers had more health problems than the other groups. On the assessment of home safety, unsafe conditions were more frequently found in the beneficiary group. The caregivers in the beneficiary group showed lower child rearing confidence than the other groups, and wanted customized visiting health care services most in the areas of developmental screening, regular health check-up and counseling, nutritional supplementation, and oral health care. Conclusion: These results indicate that it is necessary for children and parents in poverty to be provided with professional home visiting interventions for the promotion of child health and prevention of developmental problems and diseases. These findings can be used for developing future customized visiting health care service programs for infants and children in this community area.

• 대한지역사회영양학회지
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• 제9권5호
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• pp.555-565
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• 2004

The objective of this study was conducted to investigate growth and development status of infants fed soy-based formulas over 3 months. The height and weight were measured and Z-scores were calculated by using standard of the same age groups. Their mothers were interviewed using questionnaires including general and environmental characteristics, total food intakes, soy based formula intakes and Ewha infant development screening test. Main results were as follows: 1) Nutrient intake levels of subjects were similar to or more than the level of Korean Recommended Dietary Allowances except for intakes of vitamin E (79.89% RDA), and the average status of nutrient intakes of infants were fairly good. 2) Z-scores of height for age (HAZ) and Z-scores of weight for age (WAZ), Kaup index, WLI and Ewha Infant Developmental Screening Test score of subjects were in the normal growth range. 3) There were no significant differences among soy based formula intake percentile groups in HAZ, WAZ, Kaup index, WLI and Ewha Infant Developmental Screening Test score. 4) Total energy intake was positively correlated with HAZ (p < 0.00, WAZ (p <0.00, and WLI (p < 0.05) in infants less than 12 month. Also, soy based formula energy intake was positively correlated with HAZ (p < 0.05) in infants less than 12 month. However, energy and soy based formula intake levels of infants over 12 month were not significant among variables. Considering results of this study, infants fed soy-based formulas over 3 months showed normal growth and development status. Further studies are needed to evaluate longterm growth and development in infants fed soy based formulas.

• The Plant Pathology Journal
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• 제21권3호
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• pp.288-292
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• 2005

Pathogenesis-related protein-1a (PR-1a) is strongly induced in tobacco plants by pathogen attack, exogenous salicylic acid (SA) application and by other developmental processes. In order to develop a rapid screening system for the selection of plant defense-inducing compounds originated from various sources, we have transformed tobacco Samsun NN plants with a chimeric construct consisting of GUS $(\beta-glucuronidase)$. In the $T_1$ generation, three transgenic lines having stable GUS expression were selected for further promoter analysis. Using GUS histochemical assay, we observed strong GUS induction driven by PR-1a promoter in PR1a-GUS transgenic tobacco leaves in response to the exogenous application of SA or benzol (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), a SA­derivative compound. In addition, GUS expression was maintained locally or systemically in PR1a-GUS transgenic line $\#5\;T_2$ generation) until after 3 days when they were treated with same chemicals. Our results suggested that the PR1a-GUS reporter gene system in tobacco plants may be applicable for the large-scale screening of defense-inducing substances.