• Reproductive and Developmental Biology
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• v.33 no.4
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• pp.223-228
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• 2009

We attempted to control the maturation promoting factor (MPF) activity and investigated the subsequent reprogramming of bovine somatic cell nuclear transfer (SCNT) embryos. Serum-starved adult skin fibroblasts were fused to enucleated oocytes treated with 2.5 mM caffeine or $150\;{\mu}M$ roscovitine. The MPF activity, nuclear remodeling patterns, chromosome constitutions and development of SCNT embryos were evaluated. Methylated DNA of embryos was detected at various developmental stages. The MPF activity was increased by caffeine treatment or reduced by roscovitine treatment (p<0.05). Blastocyst development was higher in the caffeine-treated groups (27.6%) than that of the roscovitine-treated group (8.3%, p<0.05). There was no difference in the apoptotic cell index among the three groups. However, the mean cell number of blastocysts was increased in the caffeine-treated group (p<0.05). Higher methylation levels were observed in the Day 3 embryos of the roscovitine-treated group (50.8%), whereas lower methylation levels were noted at Day 5 in the caffeine-treated group (12.5%, p<0.05). These results reveal that the increase in MPF activity via a caffeine-treatment creates a more suitable condition for nuclear reprogramming after SCNT.

• Applied Chemistry for Engineering
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• v.7 no.3
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• pp.565-572
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• 1996

The optimum loading of carbon black was studied for the rubber compounds of natural rubber(NR), butadiene rubber(BR), and styrene-butadiene rubber(SBR) with different amount of oil. The optimum loading of carbon black was determined by the volume ratio of carbon black and L factor of Lee's theory. The optimum loading of carbon black was confirmed by the examination of physical properties of the rubbers. The optimum loading amounts of carbon black for the each rubber compound were 60 phr for NR, 57 phr for BR, 65 phr for SBR-A, 70 phr for SBR-B, and 76 phr for SBR-C, respectively. The optimum loading of carbon black was increased by 5 phr for every increment of 20 phr of oil content. It was revealed that the optimum loading amount of carbon black determined by L factor is closely related to the tensile strength of the rubber compounds. The optimum loading amount of carbon black was observed at the amount which shows the maximum value of tensile strength.

• YAKHAK HOEJI
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• v.45 no.2
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• pp.190-204
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• 2001

This study was conducted to investigate for its effects on reproductive and developmental toxicity of recombinant human epidermal growth factor (rhEGF) in Sprague-Dawley rats. Male rats were administered rhEGF at doses of 1, 10, 100, and 1000$\mu$g/kg/day, respective1y, by subcutaneous injection from 63 days before and throughout to mating period until the day before sacrifice. Female rats were administered rhEGF at the same doses from 14 days before mating to day 20 of gestation or to day 21 of lactation. We examined the male and female fertility indices and maternal toxicity of F0 parental animals. Also, we examined the external, visceral, or skeletal malformation of fetuses, growth and development, behavior, and/or reproductive performance of F1 animals. At the highest dose (1,000 $\mu$g/kg), the mean body weights of F0 animals were significantly increased in males and females at 3 or 2 weeks after treatment, respective1y. No clinical signs and food intakes were observed at any time during the experimental period by rhEGF treatment. In autopsy examination, the relative and absolute liver weights significantly increased in both sexes of 1,000 $\mu$g/kg. At the highest dose (1,000 $\mu$g/kg), there was a statistically significant increase of pregnancy period and the number of dead fetuses. Moreover, significant increase of mean fetal body weight and decrease of number of live fetuses, which related to the difficult dilivery were observed in highest dose group. In Fl examination, no adverse effects on external, visceral, and skeletal malformation, physical and functional development, behavior or reproductive ability of Fl animals were observed in any group. Also, there was no significant difference between control and treated groups in copulation or fertility indices of Fl animals. These results indicate that rhEGF had no adverse effect on fertility and reproductive ability of Sprague-Dawley rats.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.5
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• pp.261-266
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• 2003

Type 1 diabetes is an organ-specific autoimmune disease caused by the cytotoxic T cells-mediated destruction of the insulin-producing beta cells in the Langerhans pancreatic islets. Hepatocyte growth factor (HGF) is a potent mitogen and a promoter of proliferation of insulin producing beta cells of pancreatic islets. To study the role of HGF via viral vector in the development of streptozotocin (STZ)-induced diabetes in mice, we have developed an adenoviral vector genetically engineered to carry the gene for human HGF (hHGF) and evaluate the change of blood glucose, insulin level, and insulin-secreting beta cells of pancreatic islets. We demonstrate that the treatment with hHGF gene prevented the development of STZ-induced diabetes and increased serum insulin level to above normal range. Furthermore, it preserved pancreatic beta cells from destruction. These in vivo results may support previous findings that HGF is insulinotropic agent for beta cells and HGF treatment renders the cells to be resistant to the development of diabetes from STZ administration. We suggest that an adenoviral mediated hHGF gene therapy is a good candidate for the prevention and treatment of type 1 diabetes.

• Journal of Digital Convergence
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• v.14 no.12
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• pp.165-172
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• 2016

Since the advent of digital games with hardware development a key component of the game characters are changed to retard the rate at which types of users based on their favorite theory of human proportion. Many popular to gamers in the game according to the change in the retard rate in a unique image of the character. Studies on the head and body proportion are thought that up to now been insufficient. The purpose of this study is to examine a fundamental human proportion that has importance as a molding factor in the game character development, it investigated the characteristics of the game character, and classifying the retard rate of the game character, through the research method of analyzing the results, into the retard rate look for and investigate studying the effect on future game images. Five types of game characters that make up the analysis were analyzed with a unique image, and the more specialized users prefer retard rate, the figurative exaggeration to retard rate than the actual main body. Utilizing a bit exaggerated the difficulties of the character looks relatively small proportion character in the graphic wallpaper increased visibility. The proportion of a game character through the study is considered as a very important factor as the game image in the game development.

• Fashion & Textile Research Journal
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• v.15 no.5
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• pp.694-703
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• 2013

A variable dress design can be an alternative to satisfy a consumer need for diverse expression and self-realization at a lower cost factor. In the area of wedding dress, the change in the trend of wedding culture (which tends to demand more units of wedding dress) makes the cost factor more important in the purchase selection. A variable design has a clear advantage for wedding dresses and the wedding industry. This is the first research on a variable design that focuses on wedding dresses. This research develops a variable wedding design which respects consumer preferences independent of a variable wedding dress design that presents a new shape of silhouette or the development ofa new wedding dress materials. A survey on the supply side was conducted to examine market preferences by first browsing the Naver portal site and then checking the websites of major wedding dress suppliers. A questionnaire survey was conducted with a sample of 348 brides-to-be that inquired on wedding dress selection factors and purchase patterns. The survey shows that consumers prefer mermaid and A-line silhouettes, silk material, white-ivory color, and tube top necklines. The result conforms to the types commonly found in the designs of suppliers. We apply a detachable design to a basic mermaid silhouette and implemented change for 7 kinds of styles -based on the result of the survey. We suggest a variable wedding dress design as a new means to solve the cost concern and the customer need for diverse expression. The research represents a new life style for wedding culture and facilitates the development of the wedding industry.

• Clinical and Experimental Reproductive Medicine
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• v.47 no.2
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• pp.140-146
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• 2020

Objective: To investigate whether the degree of post-warming embryo or blastocyst development is associated with clinical pregnancy in vitrified embryo or blastocyst transfer cycles. Methods: Ninety-six vitrified cleavage-stage embryos and 58 vitrified blastocyst transfer cycles were selected. All transfer cycles were performed from February 2011 to March 2019, and all vitrified embryos or blastocysts were warmed from 4 PM to 6 PM and then transferred the next morning from 9 AM to 10 AM. The scores of the cleavage-stage embryos and blastocysts were assessed at warming and at transfer using the modified Steer method and the Gardner method, respectively. The mean embryo or blastocyst score, score of the single top-quality embryo or blastocyst, and the difference in the score between warming and transfer were compared between nonpregnant and pregnant women. Results: In the cleavage-stage embryo transfer cycles, both the top-quality embryo score at transfer and the difference in the score between warming and transfer were significantly associated with clinical pregnancy. A top-quality embryo score at transfer of ≥ 60.0 (area under the curve [AUC], 0.673; 95% confidence interval [CI], 0.531-0.815) and a difference in the score between warming and transfer of ≥ 23.0 (AUC, 0.675; 95% CI, 0.514-0.835) were significant predictors of clinical pregnancy. In blastocyst transfer cycles, the top-quality blastocyst score at transfer was the only significant factor associated with clinical pregnancy. A top-quality blastocyst score at transfer of ≥ 38.3 was a significant predictor of clinical pregnancy (AUC, 0.666; 95% CI, 0.525-0.807). Conclusion: The top-quality embryo score at transfer and the degree of post-warming embryo development were associated with clinical pregnancy in vitrified cleavage-stage embryo transfer cycles. In vitrified blastocyst transfer cycles, the top-quality blastocyst score at transfer was the only significant factor affecting clinical pregnancy.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.30 no.4
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• pp.175-196
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• 2019

Recently, domestic interest in gender equality is increasing, and it is expected that the demand for the collection of children's gender equality in the library will increase due to these social changes. In this study, the top 10 public libraries in Korea were selected from the list of collections of gender equality books and collection / loan data presented by various institutions such as public libraries and 'children's library research groups'. For this purpose, 10 libraries in Korea were selected and analyzed quantitatively and qualitatively through the collection status of the gender equality collections, loan status, turnover, Bonn's use Factor, and Trueswell's 80/20 rule. Based on the results of this analysis, we would like to suggest some issues to be reflected in library development policy of the future.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.2
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• pp.53-60
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• 2011

This study was conducted to investigate the effect of hydropower factors (watershed, gross head), operation ratio and unit electricity cost on the benefit-cost ratio (B/C ratio) of small hydropower using agricultural reservoirs. The equation of B/C ratio was expressed as a function of watershed area, gross head, operation ratio and unit electricity cost. The benefit increased with watershed area, gross head and unit electricity cost, while the cost increased with watershed area and gross head but decreased with operation ratio. The B/C ratio increased with watershed area, gross head, operation ratio and unit electricity cost. While the effect of gross head on the B/C ratio decreased with watershed area, the effect of operation ratio and unit electricity cost on the B/C ratio increased with watershed area. The operation ratio is an important factor to affect the B/C ratio and therefore we need to develop hydropower for the heightened dams to expect high operation ratio due to continuous water release. The unit electricity cost is also an important factor to affect the B/C ratio and the B/C ratio was always below 1 unless unit electricity cost is over 60 Won/kWh under given conditions. The reservoirs with economic feasibility for small hydropower development were three in 21 when the equation of B/C ratio was appled to the study reservoirs. The results can be used to choose the appropriate reservoir with economic feasibility for development of small hydropower.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.149-160
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• 2021

BACKGROUND: Concerns have been raised about the impact of recent high concentrations of fine dust on human health. Ammonia(NH₃) reacts with sulfur oxides and nitrogen compounds in the atmosphere to form ultrafine ammonium sulfate and ammonium nitrate (PM2.5). There is a growing need for accurate estimates of the amount of ammonia emitted during agricultural production. Therefore, in this study, ammonia emissions generated from the cultivation of leafy perilla in plastic houses were determined. METHODS AND RESULTS: Cow manure compost, swine manure compost, and poultry manure compost each at 34.6 ton ha^-1, the amount commonly used by farmers in the field, was sprayed on the soil surface. Just after spraying cow manure compost, swine manure compost, and poultry manure compost, the ammonia was periodically measured and analyzed to be 22.5 kg ha^-1, 22.8 kg ha^-1, and 85.2 kg ha^-1, respectively. The emission factors were estimated at 70.0 kg-NH₃ ton-N, 62.8 kg-NH₃ ton-N, and 234.1 kg-NH₃ ton-N, respectively. Most ammonia was released in the two weeks after application of the compost and then the amount released gradually decreased. CONCLUSION: Therefore, it is necessary to improve the emission factor through a study on the estimation of ammonia emission by type of livestock manure and major farming types such as rice fields and uplands, and to update data on the production, distribution, and sales of livestock manure.