• Reproductive and Developmental Biology
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• v.40 no.2
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• pp.23-26
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• 2016

Transcription factor called activating enhancer binding protein 2C (AP2-gamma) is found in a variety of species and expressed from oocyte stage onwards, particularly restricted to the trophectoderm. Recent studies demonstrated that ablation of Tfap2c led to failure of tight junction biogenesis, particularly the knock-down embryos of Tfap2c did not form cavity from morula to blastocyst in mouse and pig. We speculated that the Tfa2pc may also be involved in desmosome biogenesis because blastocoel formation is coincident with the establishment of desmosome. To determine this, we depleted Tfap2c injecting siRNA into one-cell zygote and analysed the expression levels of genes that are required for desmosome complex such as PkP2, Pkp3, Dsc2, and Dsg2. We found only Pkp3 was up-regulated in the knockdowned morula embryos. Interestingly, upstream region of Pkp3 had putative Tfap2c binding sites. In conclusion, our results suggest that Tfap2c is not a crucial factor but somehow it might be involved in desmosome biogenesis directly or indirectly via Pkp3.

• The Plant Pathology Journal
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• v.36 no.2
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• pp.185-191
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• 2020

Streptomyces griseus S4-7, a well-characterized keystone taxon among strawberry microbial communities, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the present study, we created a proteomics reference map for S. griseus S4-7. Additionally, analysis of differentially expressed proteins was performed against a wblE2 mutant, which was deficient in spore chain development and did not express an antifungal activity-regulatory transcription factor. We believe that our data provide a foundation for further in-depth studies of functional keystone taxa of the phytobiome and elucidation of the mechanisms underlying plant-microbe interactions, especially those involving the Streptomyces genus.

• Journal of Embryo Transfer
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• v.10 no.3
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• pp.229-236
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• 1995

The experiments reported here take advantage of the large number of in vitro matured and in vitro fertilized(IVM /IVF) bovine oocytes which can be produced, permitting the design of controlled experiments to establish a simple defined medium for the study of early embryo requirements. A total of 1,386 IVM /IVF oocytes were used to compare a simple defined medium(KSOM) with more complex culture conditions used successfully for culture of bovine embryos but do not permit study of specific requirements. All experiments were extensively replicated factorials. In Experiment 1, KSOM was superior to Menezo B$_2$ medium in producing morulae plus blastocysts from IVM /IVF oocytes(33 vs 20%, P<0.()5). The yield of morulae plus blastocysts with KSOM was 22% and with BRLC added was 30%. In Experiment 2, (a 2x2 factorial of KSOM with or without BRLC and 0, 1 ng /ml of platelet derived growth factor, PDGF) more morulae plus blastocysts (40%) were produced in KSOM-BRLC co-culture containing 1 ng /ml PDGF than in the control KSOM(12%). In Experiment 3, there was no dose response when 0, 1 and 5 ng /ml of PDGF were added. The results with simple defined KSOM medium are sufficiently promising to indicate that specific requirements of the embryo may be examined in future studies with KSOM as a base.

• The Korean Journal of Health Service Management
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• v.12 no.1
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• pp.23-34
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• 2018

Objectives : This study developed and validated the local clinic Medical Doctor Role Stress Scale(MDRS). Methods : The interviews were conducted with 12 local clinic medical doctors to develop final preliminary questions. The scale was developed using statistical item analysis, exploratory and confirmatory factor analysis surveys with local clinic medical doctors in Seoul, Busan, Daejeon, and Gyeonggi province. Results : The MDRS developed in this study consisted of 14 items and three factors. The factors were role conflict, role ambiguity and role overload. The three factors explained 56.71% of the total variance, and the internal consistency of this scale was .90. The internal consistency for each factor was .81 ~ .90. Confirmatory factor analysis through a separate sampling met the fit criteria (CFI = .941, TLI = .927, RMSEA = .079, SRMR = .069). Conclusions : The results indicate that this scale is a reliable instrument for assessing local clinic medical doctor role stress.

• Journal of Information Management
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• v.43 no.3
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• pp.97-115
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• 2012

This paper derives determining factor for the management of oceanographic data in two ways. 1) The type of oceanographic observation and the raw data which were collected from marine physics, marine chemistry, marine biology, marine geology area were analyzed. 2) The services of the KODC(Korea Oceangraphic Data Center), NFRDI(National Fisheries Research & Development Institute), KHOA(Korea Hydrographic and Oceanographic Administration) were analyzed to derive metadata elements for retrieval. After analyze, the 42 deciding factor were derived in the 9 areas (general, Observer, satellites, observation instruments, observatories, space, information, projects, and observational data, data processing).

• Journal of the Korean housing association
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• v.17 no.2
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• pp.135-142
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• 2006

The purpose of this study is to obtain the fire safety for underground commercial space with analysing fire loads and ventilation factors. It was adopted the investigation method of field survey with commercial uses for more confidential data. The results of this study can be summarized as follows; 1) The ventilation factor shows $0.04{\sim}0.24m^{1/2}$ and it may develop ventilation-controlled fires which have much fire hazard more than fuel-controlled fires. 2) The highest value of fire load is $158.48kg/m^2$ and appeared in footwear shop which has the value $137.35kg/m^2$ for unfixed combustibles and $21.13kg/m^2$ for fixed combustibles. 3) The average value of burning rates which mean the velocity of fire development and the fire damage range is 0.143. Therefore it is desired to decrease the combustibles of compartment and is needed the method of dispersion arrangement of goods and storage to steel cabinet and so on.

• The Journal of Korean Academic Society of Nursing Education
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• v.22 no.2
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• pp.137-151
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• 2016

Purpose: The purpose of this study was to develop and validate a Nurse's Character Scale for Care in Clinical settings (NCS_C). Methods: The NCS_C was developed and validated as follows: Item generation, preliminary test of questionnaire, and tests of validity and reliability. One hundred and thirty six preliminary items were developed through a literature review and in-depth interviews by 10 nurse-experts and five patients. The content validity of the items was verified through an evaluation by five nurses and seven professors in the fields of Korean literature, pedagogy and nursing. Finally, 53 items were confirmed through item analysis, factor analysis and validity tests including convergent, discriminant and concurrent validities. Data were analyzed using factor analysis, Pearson correlation coefficients, and Cronbach's alpha. Results: Thirteen factors evolved from the factor analysis, which explained 63.1% of the total variance. The factors referred to the union of two dimensions: the professional dimension and the interpersonal dimension. The internal consistency, Cronbach's alpha, was .95, and reliability of the subscales ranged from .58 to .87. Conclusion: The results of this study suggest that the NCS_C is a reliable and valid primary scale to measure nurses' or students' character level for better care in clinical settings.

• Journal of Digital Convergence
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• v.11 no.3
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• pp.101-113
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• 2013

Although the methodological literature is replete with advice regarding the development and validation of multi-item scales based on reflective measures, the issue of index construction using formative measures has received little attention. The aim of this paper is to enhance researchers' understanding of formative measures of high-order factor and assist them in their index construction efforts. This article is also to provide some insights into the nature of formative indicators for researchers to reach an informed choice as to the appropriate high order formative measurement model for their needs. We first provide a brief background on formative indicators, drawing from the limited and fragmented literature on the topic. Next we give an example of constructing an index based on actual survey data and highlight the procedures used to assess its quality. We conclude the article with some thoughts about the use of indexes in empirical studies.

• Journal of Microbiology
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• v.44 no.2
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• pp.217-225
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• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.

• Korean Journal of Human Ecology
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• v.24 no.5
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• pp.617-629
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• 2015

The purpose of this study was to explore the effects of children's self-regulation and teacher-child relationships on children's social behaviors. Participants were 239 4- to 5-year-old children(114 boys, 125 girls) and their 23 teachers. The teachers completed rating scales to measure children's social behaviors and teacher-child relationships. Children's self-regulation was assessed by a mother reported questionnaire. Data were analyzed using descriptive statistics, Pearson's correlations and stepwise multiple regression analysis. The major results of this study were as follows: First, Children's social behaviors were partially correlated with children's self-regulation and teacher-child relationships. Second, The most influential factor by children's social behaviors was teacher-child relationship. Specially, teacher-child intimacy was the most powerful factor predicting children's asocial behavior and prosocial behavior. While teacher-child conflict was the most powerful factor influencing children's aggressive behavior, hyperactive-distractible behavior and anxious-fearful behavior. Findings are discussed in terms of the importance of teacher-child relationships in the context of program development and guidance for maladjusted children.