• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.1017-1018
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• 2004

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1551-1558
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• 2014

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

• Journal of Fisheries and Marine Sciences Education
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• v.22 no.4
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• pp.589-603
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• 2010

The purpose of this study is to develop a integrated(fusion) utilization model aimed at maximizing the development/management functions of urban estuarine area and to present an integrated concept by analyzing the existing document and data. The main points of this study are as follows: 1) The integrated utilization model suggested in this paper goes beyond the existing idea of sustainable utilization and conservation, putting an emphasis on rational decision-making in estuary management and peaceful coexistence between human and nature. 2) Policies for utilization/development/conservation/regeneration of urban estuarine area include the establishment of communication system between human and nature and a safety net considering both human and nature, the support for environment-friendly community development and the importance of preserving valuable ecosystem and wetland habitat. 3) Lastly, this study suggested that the major tasks for the fusion utilization model development are the integrated management of estuary areas, the conservation and preservation of wetland ecosystem, proper utilization of estuary's productivity, and the introduction and action plans of the integrated management model including the best way to address contamination and disaster management.

• Bulletin of the Korean Chemical Society
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• v.34 no.5
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• pp.1541-1542
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• 2013

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.828-829
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• 2005

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.05a
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• pp.83-86
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• 2001

This paper aims to make a feasibility study and to propose a master plan for the development of "integrated ocean energy utilization system", which utilizes various renewable ocean energies. Fossils energy, as the most important energy resources which are inevitable for the living and industrial development, becomes exhausted and its consumption creates serious environmental problem. It is important to utilize renewable ocean energy for a sustainable and environmentally friendly development. We survey the integrated utilization of ocean energy based on surveyed energy density distribution and propose concepts of integrated ocean energy utilization plant for both onshore and offshore system. The results of this study can support national effort for renewable energy development utilizing integrated ocean energy and refer as a guideline for the technical development of sustainable integrated ocean energy.

• Bulletin of the Korean Chemical Society
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• v.32 no.4
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• pp.1368-1370
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• 2011

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.730-738
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• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.

• IEMEK Journal of Embedded Systems and Applications
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• v.6 no.3
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• pp.183-189
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• 2011

For the rapid development of embedded software, a variety of development tools have been developed. But, the embedded software developers do not use the available tools fluently and do not improve software productivity as expect because there are short of supporting utilization convenience of development tools. In this paper, we suggest a tool of building custom development environment for improving the utilization convenience of embedded software development tools. The custom development environment helps developers to rapid selection and access of appropriate tools required to develop the embedded software on the specific embedded system. In addition, it enables developers to handle the outputs from development tools associatively. Moreover it is very flexible in extension and integration because it's implementation based on Eclipse technology. We also perform the evaluation of our tool and successfully validate the utilization convenience of embedded development tools. Therefore, we expect that this tool enables developers to use the embedded development tools efficiently, and then it help improving embedded software productivity.

• Food Science of Animal Resources
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• v.40 no.4
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• pp.497-511
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• 2020

This study aimed at comparing the storage stability, fatty acids profile, volatile flavor compounds and eating quality of Hanwoo beef longissimus thoracis (LT) muscles between two marbling morphological groups (fineness and coarseness). The 1⁺ grade Hanwoo LT muscles were measured for marbling fleck morphology at the 13^th thoracic vertebrae location by using computerized image analysis. Results reveal that both marbling groups had similar proximate composition (protein, fat, moisture and collagen), color and technological quality traits (p>0.05). However, the coarse marbling group presented greater C18:2n6 and polyunsaturated fatty acids contents compared to the fine marbling group (p<0.05). Furthermore, the fine marbling group presented lower volatile basic nitrogen (VBN) and 2-thiobarbituric acid reactive substance (TBARS) contents compared to the coarse marbling group after 14 d storage (p<0.05). Also, higher amounts of C18:2n6 oxidation-derived volatile compounds such as hexanal and 3-ethyl-2-methyl-1,3-hexadiene, were found in the coarse marbling group. In term of sensory aspects, the finely-marbled group received higher scores of flavor and taste compared to the coarse marbling group (p<0.05). Overall, the marbling flecks morphology partially showed its effects on the storage stability, fatty acids profile and eating quality of the 1⁺ grade Hanwoo beef LT muscle.