• Title/Summary/Keyword: Developing embryo

Search Result 167, Processing Time 0.042 seconds

Studies on the Toxicity of Alcohol in the Developing Chick Embryo

  • Kim Jin Sik;Kim Su Won;Ryu Hye Myung;Nam Jin Sik;Min Byung Tae;Park Soo Hyun;Jeon Jung Tae;Yoo Min
    • Biomedical Science Letters
    • /
    • v.10 no.3
    • /
    • pp.305-308
    • /
    • 2004

  • We have examined alcohol-induced malformation of chick embryo. Alcohol was considered to induce the malformation of developing embryo and to have bad effects on embryonic stage. We injected alcohol into air sac on day 4 of incubation. Ten ％ alcohol-treated group showed a little decrease on their body length compared to the untreated group and distilled water-treated group. Thirty ％ alcohol-treated group showed significant decrease on their body length compared to the untreated group and 10％ ethanol treated group. In addition, we have observed malformation of eyeballs and bills. These results indicate that alcohol affects chicken developments and brings on malformation of developing stage.

  • PDF

TERATOGENICITY STUDY OF SODIUM GLYCYRRHIZINATE IN THE DEVELOPING CHICK EMBRYO

  • Park, Jae-Jun;Lee, Yong-Soon;Ahn, Hee-Yul
    • Toxicological Research
    • /
    • v.5 no.2
    • /
    • pp.167-171
    • /
    • 1989

  • Potential teratogenicity of sodium glycyrrizinate in developing chick embryo was investigated. Body length was shortened significantly by dosage related when compared to untreated and vehicle control, but there was no significant difference on body weight, hind-limb length, claw length in all of the groups.

  • PDF

Congenital Malformation Caused by Bisphenol A in Developing Chick Embryo

  • Kim Su Won;Kim Jin Sik;Ryu Hye Myung;Nam Jin Sik;Cheigh Hong Sik;Min Byung Tae;Park Soo Hyun;Yoo Min
    • Biomedical Science Letters
    • /
    • v.10 no.4
    • /
    • pp.397-401
    • /
    • 2004

  • We have examined congenital malformation in developing chick embryo caused by endocrine disruptor, bisphenol A (BPA). We injected BPA into the air sac of developing egg on day 4 of incubation. BPA-treated group with a concentration of 10 ㎍/egg showed a little decrease on their body length compared to the untreated group. But the group treated with 50㎍/egg revealed severe malformation in eyeballs and bills. The group treated with 100㎍/egg could not continue their development after few days of incubation. These results indicate that BPA clearly inhibits the normal development in chick and it should be toxic to the developing fetus at early stage and in various tissues. The study should contribute to the understanding of toxic effect of BPA in developing human fetus when exposed to the BPA.

  • PDF

Sex Linked Developmental Rate Differences in Murrah Buffalo (Bubalus bubalis) Embryos Fertilized and Cultured In Vitro

  • Sood, S.K.;Chauhan, M.S.;Tomer, O.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.12 no.1
    • /
    • pp.15-21
    • /
    • 1999

  • The aim of the present study was to determine the effect of paternal sex chromosome on early development of buffalo embryos fertilized and cultured in vitro. Embryos were produced in vitro from abattoir derived buffalo oocytes. The cleaved embryos were cocultured with buffalo oviductal epithelial cells and evaluated on day 7 under the phase contrast microscope to classify development. The embryos which reached the morula/blastocyst stage were fast developing, the embryos which were at 16-32 cell stage were medium developing and the embryos below 16 cell stage were slow developing. The embryos which showed some fragmentation in the blastomeres or degenerated blastomeres, were degenerating. Sex of emberyos (n=159) was determined using PCR for amplification of a male specific BRY. 1 (301 bp) and a buffalo specific satellite DNA (216 bp) fragments. The results thus obtained show that 1) X and Y chromosome bearing sperms fertilize oocytes to give almost equal numbers of cleaved XX and XY embryos, 2) male embryos develop faster than female embryos to reach advanced stage and 3) degeneration of buffalo embryos is not linked with the paternal sex chromosome. We suggest that faster development of males is due to differential processing of X and Y chromosome within the zygote for its activation and / or differential expression of genes on paternal sex chromosome sex chromosome during development of buffalo embryos fertilized and cultured in vitro which may be attributed to a combination of genetic and environmental factors.

  • https://doi.org/10.5713/ajas.1999.15 인용 PDF