• Journal of Fisheries and Marine Sciences Education
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• v.23 no.1
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• pp.75-91
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• 2011

The purposes of this study were to compare and analyze the local primary textbooks for the subject of 'We are the First Graders'. This study analyzed the common points and the differences among three textbooks developed by the three educational bureaus of Seoul, Daegu and Busan. The criterion of analysis were scope, sequence and integration of curriculum representing the principles for developing curriculum. The results were as follows: First, the scopes of topics included in 2007 National Curriculum of 'We are the First Graders' were faithfully reflected in the three local textbooks. Second, the sequences of textbooks were generally arranged according to the order of curriculum content except for some contents. As for 'Wise School Life', some contents were presented at the first unit of the three textbooks and the others were gradually expanded at the latter of the textbooks. Third, the integration of curriculum appeared meaningfully in the three textbooks. Based on these results, this study suggested some recommendations on the activites for the adaptation at the early stage in the primary school under 2009 National Curriculum.

• International Journal of Oral Biology
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• v.46 no.3
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• pp.140-145
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• 2021

This study aimed to develop Lautropia mirabilis-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used in designing of the qPCR primers, RTLam-F4 and RTLam-R3. The specificity of the qPCR primers were performed by conventional PCR with 37 strains of 37 oral bacterial species, including L. mirabilis. The sensitivity of the primers was determined by qPCR with the serial dilution of purified genomic DNA of L. mirabilis KCOM 3484, ranged from 4 ng to 4 fg. The data showed that the qPCR primers could detect only L. mirabilis strains and as little as 40 fg of genome DNA of L. mirabilis KCOM 3484. These results indicate that this qPCR primer pair (RTLam-F4/RTLam-R3) may be useful for species-specific detection of L. mirabilis in epidemiological studies of oral bacterial infectious diseases such as periodontal disease.

• Journal of Korean Port Research
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• v.11 no.1
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• pp.65-73
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• 1997

The objective of this paper is to suggest a decision support system allowing automatic loading and discharging planning for tanker ships, and provide a working prototype. Particular attention is placed on developing a feasible system for improving the speed and accuracy of cargo planning by combining practice and theoretical solution methods. The system is consisted of a simulation model for tank allocation and an expert system for working sequence. Several program tests are undertaken with randomly generated input data. The results suggest that the system is quite meaningful even though there are still some problems to be solved. The paper concludes with a discussion of issues for future development of the decision support system.

• Journal of the Korean Society for Precision Engineering
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• v.12 no.8
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• pp.106-113
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• 1995

Surface mounters are used to mount the parts of electronic products on PCB(Printed Circuit Board. Those machines rely on high precision, high productivity, and software for accomodation of use. In this paper, two algorithms and a software which can give the users the acco-modation and high productivity for rotary type surface mounters are presented. The algo- rithms for development of of the software consider all factors which affect the effeciency of the operation. And the interface between user and machine for the accomodation of use can be achieved through the developed software.

• Animal Bioscience
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• v.36 no.10
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• pp.1517-1529
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• 2023

Objective: The objective of this study was to investigate the phylogenetic and expression analysis of the angiopoietin-like (ANGPTL) gene family and their role in lipid metabolism in pigs. Methods: In this study, the amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. According to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: The sequence length of ANGPTLs in pigs was between 1,186 and 1,991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene shared a common origin. Quantitative reverse-transcription polymerase chain reaction analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 in the liver and ANGPTL4 in the liver, intestine and subcutaneous fat of Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusion: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species, it will also help to better understand the role of ANGPTL3, ANGPTL4, and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for developing strategies to improve meat quality of pigs.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.8A
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• pp.1149-1155
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• 1999

LEO(Low altitude Earth Orbit) Satellite systems have been utilized in the field of earth and scientific observation (cartography mission, ocean color monitoring, bioglogical coeanography, space environments observation by space physics sensor, and meteorological observation, atmospheric observation etc.), and the field of military (military communications and secret information, enemy reconnaissance etc.), and recently been developing in the field of mobile satellite commnication of GMPCS for commercial utilization. In Korea, KOMPSAT I satellite and ground system are been developing and planed to be lunched on October 1999 In this paper, the link budge of the TT&C system for LEO satellite is described and the relations between elevation angle and pass time of LEO satellite are calculated according to satellite moving. And the packet error rates of receiving data are derived three packet error rates(PER) of real-time(RT) mode, playback(PB) mode, and real-time and range tone(RT+RNG) mode are estimated according to pass time of satellite. The results of PER are the best at real-time and the worst at real-time mode and range mode at the all pass time of satellite. The average error free packet(EFP)s of real-time mode, playback mode, and real-time and range tone for the pass time of satellite are obtained as 99.999999%, 99.999912%, 99.995945% respectively. Therefore, transmission sequence of telemetry data are determined such as PER sequence according to pass time, namely, real-time, playback, and real-time and range mode.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.292-297
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• 2022

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

• Development and Reproduction
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• v.16 no.4
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• pp.301-307
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• 2012

Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

• Journal of Educational Research in Mathematics
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• v.18 no.3
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• pp.373-389
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• 2008

In this paper, we designed a teaching unit for the learning mathematization of secondary pre-service teachers through exploring the relationship between partition models and generalized fibonacci sequences. We first suggested some problems which guide pre-service teachers to make phainomenon for organizing nooumenon. Pre-service teachers should find patterns from partitions for various partition models by solving the problems and also form formulas front the patterns. A series of these processes organize nooumenon. Futhermore they should relate the formulas to generalized fibonacci sequences. Finding these relationships is a new mathematical material. Based on developing these mathematical materials, pre-service teachers can be experienced mathematising as real practices.

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.124-128
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• 2003

In order to isolate and characterize a novel fungal strain capable of producing cellulase, each samples of the old rice straw, soil, and the old tree were screened by congo red test. One of the fungi screened has been identified as Aspergillus tubingensis strain from the results of the phylogenic analysis based on partial DNA sequence and the basis of its biochemical properties. A carboxymethyl cellulase activity of the strain was higher than that of A. oryzae KCTC 6291. In CMCase activity measurement, it wasn't sensitive about pH 2.0, 3.0, 4.0, but the enzyme was more stable than A. oryzae under the various pH and temperature conditions and the enzyme activity was more similar to neutrality and alkali. Therefore, it could be suggested that the isolated strain has a potential possibility for the developing of the probiotics.