• Journal of Life Science
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• v.20 no.6
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• pp.853-860
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• 2010

The DevSR two-component system is a major regulatory system involved in redox sensing in Mycobacterium smegmatis. The DevSR system consists of the DevS histidine kinase and its cognate DevR response regulator. When exposed to hypoxic conditions, the DevS histidine kinase is activated to phosphorylate the DevR response regulator, leading to the transcriptional activation of the DevR regulation. The ligand-binding state of the heme embedded in the N-terminal GAF domain of DevS determines the kinase activity of DevS. In this study, we demonstrated that the redox-responsive cysteine (C547) in the C-terminal kinase domain is involved in the redox-dependent control of DevS kinase activity. The formation of an intersubunit disulfide bond between the C547 residues in the presence of $O_2$ led to inactivation of DevS kinase activity. The reduction of the oxidized DevS with reductants such as $\beta$-mercaptoethanol and dithiothreitol resulted in the restoration of DevS kinase activity. It was demonstrated in vivo by complementation test that the substitution of C547 to alanine partially impaired the sensory function of DevS in M. smegmatis.

• Molecules and Cells
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• v.40 no.9
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• pp.632-642
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• 2017

The DevSR (DosSR) two-component system, which is a major regulatory system involved in oxygen sensing in mycobacteria, plays an important role in hypoxic induction of many genes in mycobacteria. We demonstrated that overexpression of the kinase domain of Mycobacterium tuberculosis (Mtb) PknB inhibited transcriptional activity of the DevR response regulator in Mycobacterium smegmatis and that this inhibitory effect was exerted through phosphorylation of DevR on Thr180 within its DNA-binding domain. Moreover, the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.

• Proceedings of the Korean Society of Computer Information Conference
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• 2019.07a
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• pp.127-128
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• 2019

본 논문에서는 SR DEVS에서 부모 객체가 자식 개체로의 상속에서 입력 함수와 출력 함수의 상속 형태에 대한 특정한 상황을 고려한다. 히든 상속의 경우 자식 객체는 부모 객체로부터 부모의 자산을 상속받더라도 상속 함수에 대한 작용이 일어나지 않는다. 이 경우 부모에게서 넘겨받은 자산에 대해 상속받지 못한 것은 아니며 단지 숨겨진 상태를 의미한다.

• Proceedings of the Korean Society of Computer Information Conference
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• 2020.01a
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• pp.253-254
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• 2020

본 논문에서는 SR DEVS에서 객체간의 상속이 발생할 경우 상속 확률 변수에 대해 고려한다. 객체간 상속의 경우 상속 강도는 확률 변수에 의해 결정될 수 있다. 본 논문에서는 확률 변수로 베르누이 확률 분포를 고려하여 객체 상속에 대한 상속 강도를 고려한다.

• Journal of Life Science
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• v.24 no.12
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• pp.1330-1338
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• 2014

In Mycobacterium tuberculosis H37Rv 16 adenylyl cyclase (AC) genes have been identified, while 10 AC genes have been found in non-pathogenic Mycobacterium smegmatis. Expression of 6 AC genes (MSMEG_0218, MSMEG_3243, MSMEG_3780, MSMEG_4279, MSMEG_4477, and MSMEG_6154) among 10 AC genes in M. smegmatis was increased when M. smegmatis was subjected to hypoxic growth conditions. On the other hand, only MSMEG_3780 and MSMEG_4279 were slightly induced in the presence of NO. The cAMP levels in cells and culture media were 450- and 9.8-fold increased, respectively, when M. smegmatis was grown under hypoxic conditions relative to those grown aerobically. Intracellular levels of cAMP were increased 5.8-fold on the exposure to NO. The DevSR two-component system is known to be involved in the induction of many genes whose expression is induced under hypoxic conditions and in the presence of NO. Expression of 6 hypoxically induced AC genes was observed to be induced in a devR deletion mutant grown under hypoxic conditions, indicating that the induction of the 6 AC genes under hypoxic conditions is independent of the DevSR two-component system. In order to identify a trans-acting regulatory element that is pertinent in the hypoxic induction of MSMEG_3780, ligand-fishing analysis was performed using the upstream DNA of MSMEG_3780 and MSMEG_5136 protein was identified.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.1493-1496
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• 2012

소프트웨어 생명주기 중 가장 많은 비용이 유지보수 개발에서 발생한다. 대형 소프트웨어 시스템의 운영 유지보수 표준은 ISO/IEC 20000 으로 프로세스통합운영 및 개선활동 중심이어서 유지보수 개발에 대한 SR(Service Request) 처리시 개발 표준 및 구체적 절차가 미비하다. 그러나 대형 소프트웨어 시스템 구축 개발 시 많이 채택하고 있는 CMMI-DEV 표준을 ISO/IEC 20000 유지보수 조직 및 팀원이 내제화를 함으로써 유지보수 개발의 상호 보완적 프로세스로 명세 명확화 및 품질을 제고할 수 있고, 생산성과 효율성을 증대할 수 있다. 따라서 본 연구에서는 ISO/IEC 20000 유지보수 조직이 운영하고 있는 표준절차에 CMMI-DEV 표준 프로세스를 상호보완적으로 매칭한 표준절차를 적용한 통합모델을 제안한다. ISO/IEC 20000 모델의 개발단계에 CMMI-DEV 프로세스 모델을 적용한 통합 모델을 구축하고 실제 프로젝트에 적용하여 본 연구의 실효성을 검증한다.

• Proceedings of the Materials Research Society of Korea Conference
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• 1998.08a
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• pp.65.1-65
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• 1998

• Proceedings of the Materials Research Society of Korea Conference
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• 1998.08a
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• pp.65.2-65
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• 1998

• Journal of the Korea Society of Computer and Information
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• v.20 no.1
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• pp.137-142
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• 2015

In the object inheritance of SR DEVS, partial or overall asset inheritance can be possible. To a asset inherited from parent object, by respective dedicated function a child object can implement partial or whole asset, or not. Even though a child has a inherited asset, if the asset is the hidden property its implementation will not be provided. A inherited asset cannot be showed in whole asset implementation, or it can be implemented by special system state. Here, the whole asset implementation means may include time parameter. In this paper, we describe a determination probability scheme for partial or whole asset inherited from the parent object to determine the hidden inheritance. By the determination probability function it is decided that the inherited asset will be hidden or normal asset.