• 대한의생명과학회지
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• 제14권3호
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• pp.193-198
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• 2008

In order to evaluate on the effect of kaempferol on the cytotoxicity of oxygen tree radicals, XTT assay was performed to determine the cell viability after skin fibroblasts derived from human (Detroit 51) that were treated with various concentrations of hydrogen peroxide $(H_2O_2)$. And also, the effect of kaempferol on the cytotoxicity induced by H202 that was examined by cell viability, lactate dehydrogenase (LDH) activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in these cultures. $H_2O_2$ decreased cell viability in dose-dependent manner in these cultures and the $XTT_{90}\;and\;XTT_{50}$ values were determined at concentration of $35{\mu}M\;and\;90{\mu}M$ of $H_2O_2$ after skin fibroblasts derived from human were treated with $15{\sim}90{\mu}M$ of $H_2O_2$ for 6 hours, respectively. $H_2O_2$ was highly toxic on cultured skin fibroblasts derived from human by toxic criteria of Brenfreund and Puerner (1984). In the protective effect of kaempferol on $H_2O_2$-induced cytotoxicity, kaempferol increased DPPH radical scavenging activity and significantly decreased LDH activity. From these results, it is suggested that oxygen tree radical, $H_2O_2$, was highly toxic on cultured skin fibroblasts derived from human, and also kaempferol of flavonoid showed the protection on $H_2O_2$-induced cytotoxicity.

• 대한공간정보학회지
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• 제12권3호
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• pp.43-50
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• 2004

본 연구에서 교통수요 예측기법 중 통행량 분포기법의 이론적 배경에 대하여 광주광역시를 중심으로 컴퓨터 시뮬레이션을 통하여 분석 평가하고 모형에 내재되어 있는 매개변수의 특성변화를 연구하여 통행량 분포모형의 적용 타당성을 찾고자 하는데 연구의 목적이 있다. 본 연구에서는 통행분포모형의 정립을 목적으로 광주시의 20개 대존 중에서 도심지역에 해당되는 9개 존을 중심으로 한 통행목적별, 수단별, 출발 도착통행량 모형을 정립하였다. 여기에서는 기준 년도를 1996년으로 하고 2001년까지의 통행량을 분석하고 2008년도까지의 통행분포량을 예측하였다.

• 대한의생명과학회지
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• 제13권4호
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• pp.355-360
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• 2007

In order to evaluate on the cytotoxicity of methyl mercury (MM) and antioxidant effect of phenolic compound, poncirin against MM-induced cytotoxicity, XTT assay was performed to determine the cell viability after human skin fibroblasts (Detroit 51) were grown in the media containing various concentrations of methylmercuric chloride (MMC). And also, the antioxidant effect of poncirin on the cytotoxicity induced by MMC was examined by cell viability and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in these cultures. MMC decreased cell viability in dose-dependent manner in these cultures and the midcytotoxicity value was determined at concentration of 30 ${\mu}M$ MMC after human skin fibroblasts were treated with $10\sim50{\mu}M$ MMC for 72 hours, respectively. MMC was highly toxic on cultured human skin fibroblasts by toxic criteria. MMC-mediated cytotoxicity was related with oxidative stress by the diminution of toxic effect according to the treatment of vitamin E. In the antioxidant effect of poncirin, it showed vitamin E-like DPPH radical scavenging activity at 90 ${\mu}g/ml$ poncirin and also, remarkably increased cell viability compared with MMC-treated group. From these results, it is suggested that MMC-mediated cytoxicity was highly toxic and was related with oxidative stress in cultured human skin fibroblasts, and also phenolic compound such as poncirin showed the protection on MMC-induced cytotoxicity by antioxidant effect in these cultures.

• 설비공학논문집
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• 제23권11호
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• pp.747-753
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• 2011

The purpose of this study was to quantify the impact of the ventilation rate on heating and cooling energy consumption in a detached house. For it, a series of simulations for the application of the diverse ventilation rate (ACH) were computationally conducted for a prototypical detached residential building in the cold climate (Detroit, Michigan) and hot/humid climate (Miami, Florida) of USA. Analysis revealed that ventilation is a significant heat losing source in the cold climate; thus, the higher ventilation rate significantly increases the heating energy consumption and energy cost in the cold climate; while the impact on energy increase for heating and cooling energy consumption is similar in hot/humid climate with less significancy compared to cold climate. The research outcome of this study could be a fundamental data for determining the optimal ventilation rate in terms of indoor air quality, but also building energy performance well.

• 한국주거학회논문집
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• 제24권3호
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• pp.85-94
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• 2013

This research investigates and characterizes lifestyles and housing preferences of Korean-American Baby Boomers in the USA, and proposes future housing design and planning directions to meet their needs. A questionnaire survey was conducted from June to September 2012 examining lifestyles and preferred characteristics of postretirement homes. We targeted Korean-Americans born from 1955 to 1963 in either Korea or the USA who currently reside in New York, Washington DC, Chicago, Dallas, Detroit, Lansing, Grand Rapids, and San Francisco. To analyze the 247 responses, we employed factor analysis, cluster analysis, one-way ANOVA, and crosstabs. Respondents preferred three-bedroom, singlefamily housing types with a size of 26-35 pyong, favoring city outskirts or suburbs over urban areas. Four groups having different lifestyle types were identified: innovators, believers, fashion experiencers, and makers. Housing preference differed depending on lifestyle types. The 'innovator' group desired homes 56 pyong or larger, while the other groups preferred 26-36 pyong. The four lifestyle groups did not show statistically significant differences in most of preferred housing features, community facilities, or indoor environmental characteristics. Each group still showed slightly different preferences in some housing planning characteristics, which future planners can refer to when providing postretirement homes for them.

• 문화기술의 융합
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• 제5권4호
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• pp.131-136
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• 2019

테크노팝이라는 용어가 40년전 처음으로 사용된 이후 테크노 음악은 기술의 진보와 함께 많은 발전을 거듭했다. 그러나 테크노 음악을 음악사적 관점에서 고찰한 연구는 적은 편이며, 특히 그 태생적 특성과 관련하여 산업 기술적 측면의 발달에 대해서 이해해 볼 필요가 있다. 이에 본 논문에서는 테크노가 본격 태동하던 시기의 디트로이트 테크노와 관련한 역사적 맥락을 살펴보고 특히 큰 성공을 거둔 유럽의 테크노 문화, 그리고 테크노 음악의 산업적 의의에 대해 살펴보고자 한다. 또한 테크노 음악의 발전과 관련한 제언을 제공하고자 한다.

• 한국의상디자인학회지
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• 제23권4호
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• pp.95-103
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• 2021

This study aims to examine the antibacterial effects of cotton and silk fabrics naturally dyed with Artemisia princeps extract on antibiotic-resistant strains of bacteria. The concentrated natural dye of the Artemisia princeps extract was made at the liquor ratio of 1:10 at 40-60℃ for 60 minutes. The concentration of FeSO4·7H2O, Al2(SO4)3, and CuSO4 5H2O mordant was 3% (owf), and the liquor ratio was 1:20. In order to experiment on the antimicrobial activity of the naturally dyed fabrics, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591, was used by breeding it in Brain Heart Infusion Agar (BHA) containing Oxacillin (2㎍/ml), Fungizone (2.5㎍/ml), and Brain Heart Infusion broth (BHI; Detroit, MI, USA). As a result of examining the bacterial growth reduction rate on dyed cotton and silk fabrics against antibiotic-resistant strains, it was found that the copper mordant in cotton fabric shows the highest antibacterial activity with a bacterial growth reduction rate of 99.9%, and the non-mordant cotton fabric shows the lowest antibacterial activity with a reduction rate of 18.6%. In the case of the naturally dyed silk fabric, it indicates the highest reduction rate of strains in the Al mordanting (94.9%), and Cu mordanting (99.9%).

• Journal of the Korean Wood Science and Technology
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• 제46권2호
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• pp.166-177
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• 2018

Plant essential oils are defined as fragrant volatile oils extracted from leaves, stems, fruits, flowers, and roots of a plant. Such oils are composed of multiple components and multiple functions. By accumulation of inductive information, various plant essential oils have been studied for using in therapeutic medicine for various diseases. Despite of the apparent advantages of essential oils as a source of therapeutic medicines, plant essential oils have many limitations, including cytotoxic side effects. Therefore, it is necessary to evaluate the toxicity and the mechanisms of cytotoxicity of such oils. In this study, we evaluated the cytotoxicity to human-derived cell lines of 10 plant essential oils provided by National Institute of Forest Science (i.e., Larix kaempferi; Abies holophylla; Zanthoxylum ailanthoides; Pinus parviflora; Tsuga sieboldti; Chamaecyparis pisifera; Cryptomeria japonica; Pinus densiflora; Illicium anisatum; Pinus thunbergii). Cytotoxicity evaluations were accomplished by using CCK-assays and PCR-based cytotoxicity-related marker gene analyses with A549 cell line, and the Detroit551 cell line which are lung and skin cell line. The genes were analyzed included caspase-3 has a role in cell apoptosis, and the other cyclinA, cyclinB, cyclinD, and cyclinE regulated cell cycling for the cell proliferation. By examining the five cytotoxicity-related marker genes by performing real-time PCR and examined the cytostatic gene regulation associated with the various essential oils. The results of this study showed that the degree of cytotoxicity and the cytostatic gene regulation which could give precious information for using the plant essential oil for the clinical usages.

• 동의생리병리학회지
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• 제22권1호
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• pp.115-119
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• 2008

This study was done to evaluate the antioxidant effect of Crataegi Fructus (CF) extract on the oxidative stress induced by reactive oxygen species (ROS), The human skin fibroblasts (Detroit 551) were cultured with various concentrations of hydrogen peroxide $(H_2O_2)$. The cytotoxicity of $H_2O_2-induced$ oxidative stress was performed by XTT assay for the cell viability according to the dose- and time-dependent treatment. For the protective effect of CF extract on $H_2O_2-mediated$ oxidative stress, cell viability, lactate dehydroganase (LDH) activity, and ferric thiocyanate (FTC) assay for the inhibitive activity of lipid peroxidation on CF extract were carried out. In this study, $H_2O_2-mediated$ oxidative stress was decreased cell viability dose-, and time-dependent manner and increased LDH activity compared with the control in these cultures. In the protective effect, CF extract increased cell viability and decreased LDH activity on $H_2O_2-mediated$ oxidative stress, especially, CF extract has antioxidant effect by the showing the inhibitive activity of lipid peroxidation by FTC assay. From these results, It is suggested that $H_2O_2-mediated$ oxidative stress was highly toxic, and also, CF extract showed the protective effect on $H_2O_2-mediated$ oxidative stress by showing the increased cell viability, decreased LDH activity and lipid peroxidation inhibition in these cultures.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.494-502
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• 2018

Breast cancer is currently the most prevalent cancer in women, and its incidence increases every year. Azole antifungal drugs were recently found to have antitumor efficacy in several cancer types. They contain an imidazole (clotrimazole and ketoconazole) or a triazole (fluconazole and itraconazole) ring. Using human breast adenocarcinoma cells (MCF-7 and MDA-MB-231), we evaluated the effects of azole drugs on cell proliferation, apoptosis, cell cycle, migration, and invasion, and investigated the underlying mechanisms. Clotrimazole and ketoconazole inhibited the proliferation of both cell lines while fluconazole and itraconazole did not. In addition, clotrimazole and ketoconazole inhibited the motility of MDA-MB-231 cells and induced $G_1$-phase arrest in MCF-7 and MDA-MB-231 cells, as determined by cell cycle analysis and immunoblot data. Moreover, Transwell invasion and gelatin zymography assays revealed that clotrimazole and ketoconazole suppressed invasiveness through the inhibition of matrix metalloproteinase 9 in MDA-MB-231 cells, although no significant changes in invasiveness were observed in MCF-7 cells. There were no significant changes in any of the observed parameters with fluconazole or itraconazole treatment in either breast cancer cell line. Taken together, imidazole antifungal drugs showed strong antitumor activity in breast cancer cells through induction of apoptosis and $G_1$ arrest in both MCF-7 and MDA-MB-231 cells and suppression of invasiveness via matrix metalloproteinase 9 inhibition in MDA-MB-231 cells. Imidazole drugs have well-established pharmacokinetic profiles and known toxicity, which can make these generic drugs strong candidates for repositioning as antitumor therapies.