• 한국산학기술학회논문지
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• 제12권6호
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• pp.2807-2814
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• 2011

본 논문은 컬러공간 특성을 이용하여 유해동영상을 식별하는 알고리즘을 개발하고, 실험을 통하여 알고리즘의 효율성을 검증한다. 유해동영상 식별 알고리즘은 2차원 투영맵에 기초하고 있다. 비디오 이미지의 컬러특성을 추출하는데 있어 2차원 투영맵은 후보 프레임을 효과적으로 추출하는데 적용되어진다. 본 연구에서는 제시된 유사도 계산 알고리즘을 이용하여 추출된 프레임과 기준 이미지 간의 유사도를 먼저 계산하고, 유사도 평가를 통하여 유해동영상 후보프레임을 식별해 내고 임계치를 적용하여 최종 판단을 내린다. 제시된 알고리즘을 적용한 실험결과, 유해동영상을 찾는데 있어 컬러히스토그램보다 본 연구에서 제안한 2차원 투영맵을 이용한 기법이 계산속도와 식별능력 면에서 더 우수함을 입증하였다.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.742-753
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• 2012

Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity. We have previously reported that the soil bacterial isolate Achromobacter sp. AO22 is multi-heavy metal tolerant and exhibits a mer operon associated with a Tn21 type transposon. The present study reports that AO22 also hosts a unique cop locus encoding copper homeostasis determinants. The putative cop genes were amplified from the strain AO22 using degenerate primers based on reported cop and pco sequences, and a constructed 10,552 base pair contig (GenBank Accession No. GU929214). BLAST analyses of the sequence revealed a unique cop locus of 10 complete open reading frames, designated copSRABGOFCDK, with unusual separation of copCD from copAB. The promoter areas exhibit two putative cop boxes, and copRS appear to be transcribed divergently from other genes. The putative protein CopA may be a copper oxidase involved in export to the periplasm, CopB is likely extracytoplasmic, CopC may be periplasmic, CopD is cytoplasmic/inner membrane, CopF is a P-type ATPase, and CopG, CopO, and CopK are likely copper chaperones. CopA, B, C, and D exhibit several potential copper ligands and CopS and CopR exhibit features of two-component regulatory systems. Sequences flanking indicate the AO22 cop locus may be present within a genomic island. Achromobacter sp. strain AO22 is thus an ideal candidate for understanding copper homeostasis mechanisms and exploiting them for copper biosensor or biosorption systems.