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Antioxidative and Antimicrobial Activities of Monascus pilosus(Corn Silage Mold) Mycelial Extract and Its Culture Filtrate (Monascus pilosus 균사체 및 배양여액의 항산화 및 항균활성)

  • Kim, Jae-Won;Lee, Sang-Il;Kim, Sung-Hwan;Lee, Ye-Kyung;Kim, Soon-Dong
    • Food Science and Preservation
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    • v.17 no.5
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    • pp.741-751
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    • 2010
  • We evaluated the nutritional value of a Monascus pilosus mycelial ethanolic extract (MEM) and culture filtrate (CFM) by determining the contents of monacolin K and citrinin, and by measuring antioxidant and antimicrobial activities. The yields of freeze-dried MEM and CFM powder were 4.02% and 3.35% of wet weight, respectively. Pigment content ($OD_{500}$ value) of MEM (0.79) and CFM (0.63) were lower than those of commercial rice beni-koji ethanolic extracts (EERB) (0.87), but were in good agreement with the L*, a*, and b* values and the hue angles of the products. The total monacolin K content of MEM (24.91 mg%) was higher than those of CFM (1.27 mg%) and EERB (14.65 mg%). However, the active monacolin K content of EERB (5.48 mg%) was higher than those of MEM (3.35 mg%) and CFM (0.4 mg%). Citrinin was not detected in any sample. The total polyphenol content of MEM (4.68%, w/w) was similar to that of CFM (4.29%, w/w), thus 13.75.20.94% higher than that of EERB. The total flavonoid content of EERB was 6.8.7.0-fold higher than those of MEM (0.64%, w/w) and CFM (0.66%, w/w). The total antioxidant capacity of CFM (3.51%, w/w) was 1.62.2.08-fold higher than those of MEM (2.74%, w/w) and EERB (1.69%, w/w). The electron-donating capacities of 1% (w/v) solutions of CFM, MEM, BHT, and EERB were 86.20%, 77.25%, 77.25%, and 44.82%, respectively, and the corresponding reducing powers ($OD_{700}$ values) were 2.1, 2.4, 1.1, and 1.6, respectively. SOD(superoxide dismutase)-like activities were in the order MEM (39.85%) > BHT (37.68%) > EERB (26.70%) > CFM (21.5%). Although the TBARS (% value) of MEM was a little lower than that of BHT, it was higher than those of CFM and EERB. The antibacterial activities of CFM acting on Bacillus brevis and Escherichia coli were somewhat higher than those of MEM, whereas the activities of MEM on Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, and Salmonella enteritidis were higher than those of CFM. However, the antibacterial activities of MEM and CFM were less than those of EERB and BHT. In conclusion, although further studies are needed, we offer experimental evidence that the by-products of M. pilosus MEM and CFM contain significant antioxidant and antimicrobial activities that may be useful in the development of healthy foods.

Site Characteristics, and Vegetation Structure, and Dynamics of Forest Communities growing Kalopanax septemlobus (Thunb.ex Murray) Koidz. in Gangwon-do (강원도 음나무 자생 임분의 입지환경, 식생구조 및 동태)

  • 이철호;최영철;김세현;권기원
    • Korean Journal of Plant Resources
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    • v.13 no.3
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    • pp.227-242
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    • 2000
  • Site characteristics, vegetation structure and dynamics of Kalopanax septemlobus forests in Mt. Heungjeong, Mt. Balwang and Mt. Gariwang were studied to provide the information need for national resources conservation and restoration of K. septemlobus forest in Gangwon-do of Korea. The K. septemlobus forests were distributed at 780m to 1,300m in altitude, steep slope, ridges-slope areas with facing northeast to northwest in good nutrition area. The annual diameter growth of those trees ranged 1.60mm to 2.41mm. Forty-three plots(20$\times$20m) were set up at three natural population of Kalopanax septemlobus forest and TWINSPAN method was used for vegetation structure analysis. The result of TWINSPAN ordination show that geographical features played a role in determining community types in the study areas. Community type of the study areas classified into three groups by TWINSPAN; K. septemlobus-Quercux mongolica- Acer mono community(Mt. Balwang), K. septemlobus-Q. mongolica- Tilia amurensis community(Mt. Heungjeong), K. septemlobus-Betula costata-Q. mongolica community(Mt. Gariwang). In most of the K. septemlobus forests, major woody species appeared predominantly were Q. mongolica, T. amurensis, Acer pseudo-sieboldianum, Lindera obtusiloba, A. mono, Magnolia sieboldii, Ulmus davidiana var. japonica. And also, Those species were surveyed in all the areas. Species diversity(H') of investigated areas ranged 0.3124~0.13992 and this value relatively higher than that of other forests with similar site. The range of similarity indices between surveyed areas was 64.1~64.54%. The distribution pattern of Morista's index show that Kalopanax septemlobus distributed randomly in tree and concentrated a few sample plots in subtree layer and shurb layer. K. septemlobus occupied 37.1~58.8% of tree layer story, 8.4~17.6% of subtree layer, 0~0.38% of shurb layer each surveyed area. The association analysis showed that Kalopanax septemlobus positively associated with Betula ermani, Deutzia glabrata, Carpinus cordata, Fraxinus mandshurica and negatively associated with Symplocos chinensis var. pilosa, Styrax obassia, Acer mono, Euonymus macroptera, Ulmus davidiana var. japonica. According to the diameter distribution of Kalopanax septemlobus community, the community of Mt. Gariwang will gradually decline and replaced by Quercus community. The communities of Mt. Heungjeong and Mt. Balwang has its seedlings and saplings, so it may be sustained.

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Statics corrections for shallow seismic refraction data (천부 굴절법 탄성파 탐사 자료의 정보정)

  • Palmer Derecke;Nikrouz Ramin;Spyrou Andreur
    • Geophysics and Geophysical Exploration
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    • v.8 no.1
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    • pp.7-17
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    • 2005
  • The determination of seismic velocities in refractors for near-surface seismic refraction investigations is an ill-posed problem. Small variations in the computed time parameters can result in quite large lateral variations in the derived velocities, which are often artefacts of the inversion algorithms. Such artefacts are usually not recognized or corrected with forward modelling. Therefore, if detailed refractor models are sought with model based inversion, then detailed starting models are required. The usual source of artefacts in seismic velocities is irregular refractors. Under most circumstances, the variable migration of the generalized reciprocal method (GRM) is able to accommodate irregular interfaces and generate detailed starting models of the refractor. However, where the very-near-surface environment of the Earth is also irregular, the efficacy of the GRM is reduced, and weathering corrections can be necessary. Standard methods for correcting for surface irregularities are usually not practical where the very-near-surface irregularities are of limited lateral extent. In such circumstances, the GRM smoothing statics method (SSM) is a simple and robust approach, which can facilitate more-accurate estimates of refractor velocities. The GRM SSM generates a smoothing 'statics' correction by subtracting an average of the time-depths computed with a range of XY values from the time-depths computed with a zero XY value (where the XY value is the separation between the receivers used to compute the time-depth). The time-depths to the deeper target refractors do not vary greatly with varying XY values, and therefore an average is much the same as the optimum value. However, the time-depths for the very-near-surface irregularities migrate laterally with increasing XY values and they are substantially reduced with the averaging process. As a result, the time-depth profile averaged over a range of XY values is effectively corrected for the near-surface irregularities. In addition, the time-depths computed with a Bero XY value are the sum of both the near-surface effects and the time-depths to the target refractor. Therefore, their subtraction generates an approximate 'statics' correction, which in turn, is subtracted from the traveltimes The GRM SSM is essentially a smoothing procedure, rather than a deterministic weathering correction approach, and it is most effective with near-surface irregularities of quite limited lateral extent. Model and case studies demonstrate that the GRM SSM substantially improves the reliability in determining detailed seismic velocities in irregular refractors.

Effect of soil-ameliorator mixtures on nutrient leaching in sandy paddy soil (사질답토양(砂質畓土壤)에 수종(數種) 개량제(改良劑) 시용(施用)이 양분용탈(養分溶脫)에 미치는 영향(影響))

  • Ahn, Sang-Bae;Park, Jun-Kyu;Yeon, Beong-Yeal;Yuk, Chang-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.131-138
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    • 1987
  • Experimental informations on the possible alternative resources of soil addition in sandy paddy soils were obtained by applying fertilizer N, P, and K to the top of 26 cm long columns containing the soil-ameliorator mixture and by determining the concentration and leaching loss of nutrients in percolated water and permeability. 1. Addition of red earth and compost to soils decreased pronouncedly the permeability. Relative magnitude of permeability was compost+slag+red earth > compost+red earth > compost > red earth > compost+slag > slag > non-added soil. 2. Concentration and leaching loss of $NH_4-N$ and $SiO_2$ were high by addition of compost-slag or red earth mixture to soils. The present of these nutrients in soils after experiment was, also, higher than that in non-added soil and in red earth to soils. 3. Those of K, Ca, and Mg were similar to $NH_4-N$ and $SiO_2$. Especially, leaching loss and present of K in soils by addition of compost to soils were higher dramatically than those of non-added soil and of red earth to soils. 4. Those of $Fe^{{+}{+}}$ in non-added soil were much higher than those by addition of compost and slag to soils. These values were the highest in 12 days after submergence, while these of $Mn^{{+}{+}}$ the lowest. 5. Concentration of $NH_4-N$ was high by addition of compost to soils, while the present of it in soils after experiment was tended to be contrary.

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Effect of Biomass and N Production by Cultivation Methods of Leguminous and Gramineae Green Manures on Rice Growth in Central Regions of Korea (중부지역 답리작에서 두과 및 화본과 녹비작물의 재배방법에 따른 biomass, 질소 함량이 벼 수량에 미치는 영향)

  • Jeon, Weon-Tai;Seong, Ki-Yeong;Kim, Min-Tae;Oh, In-Seok;Choi, Bong-Su;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.853-858
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    • 2011
  • The cultivation methods are important for determining crop yield of green manure. The effect of cultivation methods of green manure crops (GMC) on biomass and rice yield was investigated. This experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts from Oct. 2007 to Oct. 2008 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Seven GMC (hairy vetch, barley, Chinese milk vetch, rye, crimson clover, oats, rattail fescue) were cultivated and incorporated on paddy soil by broadcasting before rice harvesting (BBRH) and partial tillage seeding (PTS). Among the three leguminous GMC, the biomass and N production were the highest at the hairy vetch of PTS. Among the four gramineae GMC, the biomass and N production tended to be higher in the rye of BBRH and barley of PTS. The C/N ratio (56.5~74.2) of rye was high compared with hairy vetch (14.1). Among the GMC, the incorporation of hairy vetch increased $NH_4$-N contents in rice paddy soil at 14 and 42 days after transplanting. These results showed that hairy vetch had no significant to rice yield compared with conventional fertilization. Therefore, hairy vetch seems to be the most efficient green manure crop as an alternatives to chemical N fertilizer in the central regions of Korea.

Analysis of Fungicide Prochloraz in Platycodi Radix by GC-ECD (GC-ECD를 이용한 한약재 길경(Platycodi Radix) 중 살균제 Prochloraz의 분석)

  • Oh, Gyeong-Seok;Yoon, Myung-sub;Yang, Seung-Hyun;Choi, Hoon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.4
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    • pp.353-358
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    • 2021
  • BACKGROUND: Prochloraz has been widely used as an imidazole fungicide on fruits and vegetables in Korea. Analytical approaches to evaluate prochloraz residues in herbal medicine are required for their safety management. In this study, we developed a GC-ECD method for quantitative determination of prochloraz in Platycodi Radix. The metabolite 2,4,6-trichlorophenol (2,4,6-T) was used as a target compound to evaluate total prochloraz residues as it is categorized to a representative residue definition of prochloraz. All residues containing 2,4,6-T were converted to 2,4,6-T and subjected to GC-ECD. METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining prochloraz and it metabolite 2,4,6-T in Platycodi Radix. Prochloraz and its metabolite 2,4,6-T residuals were extracted using acetone. The extract was diluted with and partitioned directly into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was decomposed to 2,4,6-T, and then the partitioned ion-associate was finally purified by optimized aminopropyl solid-phase extraction (SPE). The limits of quantitation of the method (MLOQs) were 0.04 mg/kg and 0.02 mg/kg, respectively for prochloraz and 2,4,6-T, considering the maximum residue level (MRL) of prochloraz as 0.05 mg/kg in Platycodi Radix. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (82.1-89.7%). Good reproducibilities were obtained (coefficient of variation < 2.8%), and the linearities of calibration curves were reasonable (r2 > 0.9986) in the range of 0.005-0.5 ㎍/mL. CONCLUSION(S): The method developed in this study was successfully validated to meet the guidelines required for quantitative determination of pesticides in herbal medicine. Thus, the method could be useful to monitor prochloraz institutionally in herbal medicine.

Compositional changes in maesil-cheong formulated with turanose during the storage period (투라노스 당침을 통해 제조된 매실청의 저장기간 중 성분 함량 변화)

  • Kim, Jung-Geun;Yoo, Sang-Ho
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.688-694
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    • 2021
  • Turanose is a potential candidate for use as a functional sweetener because of its gentle taste, low calorie, and non-cariogenicity. The aim of this study was to replace sucrose with turanose to produce health-beneficial maesil-cheong. Quality effects of turanose on maesil-cheong were evaluated by determining the contents of free sugars, organic acids, amygdalin, and antioxidant activity. The pH and Brix values of sucrose- and turanose-based maesil-cheong remained at the same level between 2.83 and 3.00 and 54.6-58.6°Bx, respectively, after 90-day storage. Among oxalic, malic, and citric acids, citric acid content was the highest in both maesil-cheong samples. Turanose did not significantly hydrolyze in maesil-cheong, whereas sucrose was completely hydrolyzed to glucose and fructose. Thus, turanose is suitable for the development of acidic maesil-cheong to improve its health promoting effect. Turanose showed product qualities similar to sucrose-based maesil-cheong. Turanose can be used as a functional sweetener or bulking agent in processed foods.

Detection of Arctic Summer Melt Ponds Using ICESat-2 Altimetry Data (ICESat-2 고도계 자료를 활용한 여름철 북극 융빙호 탐지)

  • Han, Daehyeon;Kim, Young Jun;Jung, Sihun;Sim, Seongmun;Kim, Woohyeok;Jang, Eunna;Im, Jungho;Kim, Hyun-Cheol
    • Korean Journal of Remote Sensing
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    • v.37 no.5_1
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    • pp.1177-1186
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    • 2021
  • As the Arctic melt ponds play an important role in determining the interannual variation of the sea ice extent and changes in the Arctic environment, it is crucial to monitor the Arctic melt ponds with high accuracy. Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2), which is the NASA's latest altimeter satellite based on the green laser (532 nm), observes the global surface elevation. When compared to the CryoSat-2 altimetry satellite whose along-track resolution is 250 m, ICESat-2 is highly expected to provide much more detailed information about Arctic melt ponds thanks to its high along-track resolution of 70 cm. The basic products of ICESat-2 are the surface height and the number of reflected photons. To aggregate the neighboring information of a specific ICESat-2 photon, the segments of photons with 10 m length were used. The standard deviation of the height and the total number of photons were calculated for each segment. As the melt ponds have the smoother surface than the sea ice, the lower variation of the height over melt ponds can make the melt ponds distinguished from the sea ice. When the melt ponds were extracted, the number of photons per segment was used to classify the melt ponds covered with open-water and specular ice. As photons are much more absorbed in the water-covered melt pondsthan the melt ponds with the specular ice, the number of photons persegment can distinguish the water- and ice-covered ponds. As a result, the suggested melt pond detection method was able to classify the sea ice, water-covered melt ponds, and ice-covered melt ponds. A qualitative analysis was conducted using the Sentinel-2 optical imagery. The suggested method successfully classified the water- and ice-covered ponds which were difficult to distinguish with Sentinel-2 optical images. Lastly, the pros and cons of the melt pond detection using satellite altimetry and optical images were discussed.

Analyzing the Characteristics of Atmospheric Stability from Radiosonde Observations in the Southern Coastal Region of the Korean Peninsula during the Summer of 2019 (라디오존데 고층관측자료를 활용한 한반도 남해안 지역의 2019년도 여름철 대기 안정도 특성 분석)

  • Shin, Seungsook;Hwang, Sung-Eun;Lee, Young-Tae;Kim, Byung-Taek;Kim, Ki-Hoon
    • Journal of the Korean earth science society
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    • v.42 no.5
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    • pp.496-503
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    • 2021
  • By analyzing the characteristics of atmospheric stability in the southern coastal region of the Korean Peninsula in the summer of 2019, a quantitative threshold of atmospheric instability indices was derived for predicting rainfall events in the Korean Peninsula. For this analysis, we used data from all of the 243 radiosonde intensive observations recorded at the Boseong Standard Weather Observatory (BSWO) in the summer of 2019. To analyze the atmospheric stability of rain events and mesoscale atmospheric phenomena, convective available potential energy (CAPE) and storm relative helicity (SRH) were calculated and compared. In particular, SRH analysis was divided into four levels based on the depth of the atmosphere (0-1, 0-3, 0-6, and 0-10 km). The rain events were categorized into three cases: that of no rain, that of 12 h before the rain, and that of rain. The results showed that SRH was more suitable than CAPE for the prediction of the rainfall events in Boseong during the summer of 2019, and that the rainfall events occurred when the 0-6 km SRH was 150 m2 s-2 or more, which is the same standard as that for a possible weak tornado. In addition, the results of the atmospheric stability analysis during the Changma, which is the rainy period in the Korean Peninsula during the summer and typhoon seasons, showed that the 0-6 km SRH was larger than the mean value of the 0-10 km SRH, whereas SRH generally increased as the depth of the atmosphere increased. Therefore, it can be said that the 0-6 km SRH was more effective in determining the rainfall events caused by typhoons in Boseong in the summer of 2019.

Quality Evaluation through Inter-Comparison of Satellite Cloud Detection Products in East Asia (동아시아 지역의 위성 구름탐지 산출물 상호 비교를 통한 품질 평가)

  • Byeon, Yugyeong;Choi, Sungwon;Jin, Donghyun;Seong, Noh-hun;Jung, Daeseong;Sim, Suyoung;Woo, Jongho;Jeon, Uujin;Han, Kyung-soo
    • Korean Journal of Remote Sensing
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    • v.37 no.6_2
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    • pp.1829-1836
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    • 2021
  • Cloud detection means determining the presence or absence of clouds in a pixel in a satellite image, and acts as an important factor affecting the utility and accuracy of the satellite image. In this study, among the satellites of various advanced organizations that provide cloud detection data, we intend to perform quantitative and qualitative comparative analysis on the difference between the cloud detection data of GK-2A/AMI, Terra/MODIS, and Suomi-NPP/VIIRS. As a result of quantitative comparison, the Proportion Correct (PC) index values in January were 74.16% for GK-2A & MODIS, 75.39% for GK-2A & VIIRS, and 87.35% for GK-2A & MODIS in April, and GK-2A & VIIRS showed that 87.71% of clouds were detected in April compared to January without much difference by satellite. As for the qualitative comparison results, when compared with RGB images, it was confirmed that the results corresponding to April rather than January detected clouds better than the previous quantitative results. However, if thin clouds or snow cover exist, each satellite were some differences in the cloud detection results.