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Antioxidative and Antimicrobial Activities of Monascus pilosus(Corn Silage Mold) Mycelial Extract and Its Culture Filtrate  

Kim, Jae-Won (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Lee, Sang-Il (Department of Food, Nutrition and Culinary Arts, Keimyung College)
Kim, Sung-Hwan (Kyongbuk Insitute of Health, Environment)
Lee, Ye-Kyung (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Kim, Soon-Dong (Faculty of Food Science and Industrial Technology, Catholic University of Daegu)
Publication Information
Food Science and Preservation / v.17, no.5, 2010 , pp. 741-751 More about this Journal
Abstract
We evaluated the nutritional value of a Monascus pilosus mycelial ethanolic extract (MEM) and culture filtrate (CFM) by determining the contents of monacolin K and citrinin, and by measuring antioxidant and antimicrobial activities. The yields of freeze-dried MEM and CFM powder were 4.02% and 3.35% of wet weight, respectively. Pigment content ($OD_{500}$ value) of MEM (0.79) and CFM (0.63) were lower than those of commercial rice beni-koji ethanolic extracts (EERB) (0.87), but were in good agreement with the L*, a*, and b* values and the hue angles of the products. The total monacolin K content of MEM (24.91 mg%) was higher than those of CFM (1.27 mg%) and EERB (14.65 mg%). However, the active monacolin K content of EERB (5.48 mg%) was higher than those of MEM (3.35 mg%) and CFM (0.4 mg%). Citrinin was not detected in any sample. The total polyphenol content of MEM (4.68%, w/w) was similar to that of CFM (4.29%, w/w), thus 13.75.20.94% higher than that of EERB. The total flavonoid content of EERB was 6.8.7.0-fold higher than those of MEM (0.64%, w/w) and CFM (0.66%, w/w). The total antioxidant capacity of CFM (3.51%, w/w) was 1.62.2.08-fold higher than those of MEM (2.74%, w/w) and EERB (1.69%, w/w). The electron-donating capacities of 1% (w/v) solutions of CFM, MEM, BHT, and EERB were 86.20%, 77.25%, 77.25%, and 44.82%, respectively, and the corresponding reducing powers ($OD_{700}$ values) were 2.1, 2.4, 1.1, and 1.6, respectively. SOD(superoxide dismutase)-like activities were in the order MEM (39.85%) > BHT (37.68%) > EERB (26.70%) > CFM (21.5%). Although the TBARS (% value) of MEM was a little lower than that of BHT, it was higher than those of CFM and EERB. The antibacterial activities of CFM acting on Bacillus brevis and Escherichia coli were somewhat higher than those of MEM, whereas the activities of MEM on Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, and Salmonella enteritidis were higher than those of CFM. However, the antibacterial activities of MEM and CFM were less than those of EERB and BHT. In conclusion, although further studies are needed, we offer experimental evidence that the by-products of M. pilosus MEM and CFM contain significant antioxidant and antimicrobial activities that may be useful in the development of healthy foods.
Keywords
M. pilosus; monacolin K; citrinin; antioxidant activity; antimicrobial activity;
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1 Marklund S, Marklund G. (1974) Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biol. Chem., 47, 468-474
2 Middleton EJ, Kandaswami C. (1994) Potential health promoting properties of citrus flavonoids. Food Technol., 48, 115-119
3 Im MJ, Manson PN, Bulkley GB, Hoopes JE. (1985) Effects of superoxide dismutase and allopurinol in survival of acute island skin flaps. Ann. Surg., 201, 357-359   DOI   ScienceOn
4 Endo A. (1980) Monacolin K, a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase. J. Antibiot., 33, 334-336   DOI
5 Dewanto V, Wu X, Adom KK, Liu RH. (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem., 50, 3010-3014   DOI   ScienceOn
6 Izawa S, Harada N, Watanabe T, Yamamoto A, Hayatsu H, Arimoto-Kobayashi S. (1997) Inhibitory effects of food-coloring agents derived from Monascus on the mutagenicity of heterocyclic amines. J. Agric. Food Chem., 45, 3980-3984   DOI   ScienceOn
7 Yasukawa K, Takahashi M, Yamanouchi S, Takido M. (1996) Inhibitory effect of oral administration of Monascus pigment on tumor promotion in two-stage carcinogenesis in mouse skin. Oncol., 53, 247-249   DOI   ScienceOn
8 Watanabe T, Mazumder TK, Yamamoto A, Nagai S, Arimoto-Kobayashi S, Hayatsu H, Terabe S. (1999) A simple and rapid method for analyzing the Monascus pigmentmediated degradation of mutagenic 3-hydroxyamino-1-methyl -5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography. Mutat. Res., 444, 75-83   DOI   ScienceOn
9 Palo MA, Vidal-Adeva L, Maceda L. (1961) A study on ang-kak and its production. Philippines J. Sci., 89, 1-22
10 Juzlova P, Martinkova L, Kren V. (1996) Secondary metabolites of the fungui Monascus: a review. J. Ind. Microbiol., 16, 163-170   DOI
11 Par MZ, Yoon EK, Kim SD. (2002) Stability of pigment troduced by Monascus pilosus. Korean J. Food Sci. Technol., 34, 541-545   과학기술학회마을
12 Shin SR, Hong JY, Nam HS, Yoon KY, Kim KS. (2006) Anti-oxidative effects of extracts of korean herbal materials. J. Korean Soc. Food Sci. Nutr., 35, 187-191   과학기술학회마을   DOI
13 Ryu CS, Kim YB, Hwang HJ. (1995) Antimicrobial effect of Monascus strain isolated from An-Khak. J. Food Hyg. Safety, 10, 271-277
14 Mah JH, Hwang HJ. (1996) Screening of Monascus strain for antimicrobial activity and effect of change of nutrition and incubation conditions on antimicrobial activity. J. Korean Soc. Food Sci. Nutr., 25, 1080-1086
15 Nozaki H, Date S, Kondo H, Kiyohara H, Takaoka D, Tada T, Nakayama M. (1991) Ankalactone, a new ${\alpha},{\beta}$ -unsaturated $\gamma$-lactone from Monascus anka. Agric. Biol. Chem., 55, 899-900   DOI
16 Martikova L, Juzlova P, Vesely D. (1995) Biological activity of polypeptide pigments produced by the fungus Monascus. J. Appl. Bacteriol., 68, 307-318
17 Lim CM, Kyung KH, Yoo YJ. (1987) Antimicrobial effects of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Korean J. Food Sci. Technol., 19, 54-60
18 Holasova M, Fiedlerova V, Smrcinova H, Orsak M, Lachman J, Vavreinova S. (2002) Buckwheat the source of antioxidant activity in functional foods. Food Res. Int., 35, 207-211   DOI   ScienceOn
19 Kitani K, Minami C, Amamoto T, Kanai S, Ivy GO, Carrillo MC. (2002) Pharmacological interventions in aging and age-associated disorders: potentials of propargylamines for human use. Ann. NY Acad. Sci., 959, 295-307   DOI
20 Na GM, Han HS, Ye SH, Kim HK. (2004) Physiological activity of medicinal plant extracts. Korean J. Food Preserv., 11, 388-393   과학기술학회마을
21 Hur SJ, Ye BW, Lee JL, Ha YL, Park GB, Joo ST. (2004) Effect of conjugated linoleic acid on color and lipid oxidation of beef patties during cold storage. Meat Sci., 66, 771-775   DOI   ScienceOn
22 Chung JH, Ho JS, Moon CK. (1990) Direct interaction of streptozotocin with TBA(thiobarbituric acid) in lipid peroxidation analysis. Korean J. Food Hyg., 5, 237-242
23 Kang YH, Park YK, Oh SR, Moon KD. (1995) Studies on the physiological functionality of pine needle and mugwort extracts. Korean J. Food Sci. Technol., 27, 978-984
24 Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J. Food Sci. Technol., 37, 233-240   과학기술학회마을
25 Choi CH, Song ES, Kim SJ, Kang MH. (2003) Antioxidative activities of Castanea crenata Flos. methanol extracts. Korean J. Food Sci. Technol., 35, 1216-1220   과학기술학회마을
26 Torel J, Gillard J, Gillard P. (1986) Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochem., 25, 383-385   DOI   ScienceOn
27 Osawa T. (1994) Novel natural antioxidant for utilization in food and biological system. In Postharvest Biochemistry of Plant Food Material in the Tropics. Uritani I, Garcia VV, Mendoza EM, eds. Japan Scientific Societies Press, Tokyo, Japan. p. 241-251
28 Conner DE, Beuchat LR. (1984) Sensitivity of heat-stressed yeasts to essential oils of plants. Appl. Environ. Microbiol., 47, 229-233
29 Kim DS, Ahn BW, Yeum DM, Lee DW, Kim ST, Park YH. (1987) Degradation of carcinogenic nitrosamine formation factor by natural food components. Bull. Korean Fish. Soc., 20, 463-468
30 Buege JA, Aust SD. (1978) Microsomal lipid peroxidation. Meth. Enzymol., 52, 302-310   DOI
31 Yoon EK, Kim MJ, Kim SD. (2002) Growth and pigment formation of genus Monascus on medium compositions. Korean J. Food Preserv., 9, 425-428   과학기술학회마을
32 Fosell ADG, Robertson A, Whelly WB. (1956) Monascorubramine. J. Chem. Soc. Spec. Publ., 5, 27-34
33 Endo A. (1985) Trends in Monascus koji and Monascus strains. Hako To Kogyo, 43, 544-552
34 Juzlova P, Martinkova L, Kren V. (1996) Secondary metabolites of the fungus Monascus: A review. J. Ind. Microbiol., 16, 16-170
35 Manzoni M, Rollini M. (2002) Biosynthesis and biotechnological production of statins by filamentous fungi and application of these cholesterol-lowering drugs. Appl. Microbiol. Biotechnol., 58, 555-564   DOI   ScienceOn
36 Prieto P, Pineda M, Aguilar M. (1999) Spectrophotometric quantitation of antioxidant capactity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem., 269, 337-341   DOI   ScienceOn
37 Duval B, Shetty K. (2001) The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J. Food Biochem., 25, 361-377   DOI   ScienceOn
38 Youn UK, Kim YH, Kim SD. (2003) Pigment and monacolin K content of beni-koji fermented with soybean curd residue. Korean J. Food Preserv., 10, 360-364   과학기술학회마을
39 Kang SG, Jung ST. (1995) Pigment production and color difference of liquid Beni-koji under submerged cultural conditions. Korean J. Appl. Microbiol. Biotechnol., 23, 472-478
40 Roman K, Vladimir K. (1993) Determination of Lovastatin (mevinolin) and mevinolinic acid in fermentation liquids. J. Chromatogr., 630, 415-417   DOI
41 Reinhard H, Zimmerli B. (1999) Reversed-phase liquid chromatographic behavior of the mycotoxins citrinin and ochratoxin A. J. Chromatogr., 862, 147-159   DOI
42 Blois MS. (1958) Antioxidant determinations by the use of stable free radical. Nature., 181, 1199-1120   DOI   ScienceOn
43 Saeedeh AD, Asna U. (2007) Antioxidant properties of various solvent extracts of mulberry(Morus indica L.) leaves. Food Chem., 102, 1233-1240   DOI   ScienceOn
44 Martikova L, Juzlova P, Vesely D. (1995) Biological activity of polypeptide pigments produced by the fungus Monascus. J. Appl. Bacteriol., 79, 609-616   DOI
45 Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. (1987) Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem., 51, 1333-1338   DOI
46 Su YC, Wang JJ, Lin TT, Pan TM. (2003) Production of the secondary metabolites $\gamma$-aminobutyric acid and monacolin K by Monascus. J. Ind. Microbiol. Biotechnol., 30, 41-46   DOI
47 Blanc PJ, Loret MO, Goma G. (1995) Production of citrinin by various species of Monascus. Biotech. Lett., 17, 291-294   DOI   ScienceOn
48 Fink-Gremmels J, Dresel J, Leistner L. (1991) Use of Monascus extracts as an alternative to nitrite in eat products. Fleischwirtsch, 71, 329-331
49 Park MZ. (2001) Study on soy sauce preparation fermented by Monascus pliosus KCCM 60084. Dept. of Food Sci. and Technol. The Graduate School in Gatholic University of Daegu, Kyungsan, Korea
50 Ryu BH, Ahn MK, Park JO. (1995) Production of cholesterol inhbitor, monacolin produced from Monascus pilosus M015. J. Korean Soc. Food Nutr., 24, 92-97
51 Yoshimura MS, Yamanaka K, Mitsugi K, Hirose Y. (1975) Production of Monascus pigment in a submerged culture. Agric. Biol. Chem., 39, 1789-1795   DOI
52 Broder CU, Koehler PE. (1980) Pigments produced by M. purpureus with regard to quality and quantity. J. Food Sci., 45, 567-569   DOI
53 Su YC. (1983) Fermentative production of anka pigments. Korea J. Appl. Microbial. Bioeng., 11, 325-337
54 Ju JY, Nam HW, Yoon JC, Shin CS. (1994) Extractive fermentation of red pigment using Monascus sp, J101. Korea J. Appl. Microbial. Biotechnol., 22, 85-91
55 Hiroi T, Shima T, Isobe A, Kimura S. (1975) Studies on the structure of two pigment obtained from Monascus sp. J. Jpn. Soc. Food Nutr., 28, 497-502
56 Inoue K, Shirai T, Ochiai H, Kasao M, Hayakawa K, Kimura M. (2003) Blood-pressure-lowering effect of a novel fermented milk containing $\gamma$-aminobutyric acid in mild hypertensives. Eur. J. Clin. Nutr., 57, 490-495   DOI   ScienceOn
57 Lin CF, Suen SJT. (1973) Isolation of hyperpigment productive mutants of Monascus sp. F-2. J. Ferment. Technol., 51, 757-759
58 Tsukioka MT, Suzuki HT, Kono T. (1986) Pigment production by mutants of Monascus anka (Studies on alcoholic beverage production using genus Monascus, Part I. Nippon Nogeikagaku Kaishi, 60, 451-455   DOI
59 Choi CS, Jeon CP, Lee JB, Lee OS, Rhee C.H, Kwon GS. (2006) Optimal culture conditions for production of yellow pigments from Monascus purpureus in liquid culture. Korean J. Food Preserv., 13, 192-197   과학기술학회마을
60 Kim CS, Rhee SH, Kim SH. (1977) Studies on production and characteristics of edible red color pigment produced by moli (Monascus sp.). Korean J. Food Sci. Technol., 9, 277-283
61 Kim EY, Rhyu MR. (2008) Antimicrobial activities of Monascus koji extracts. Korean J. Food Sci. Technol., 40, 76-81   과학기술학회마을
62 Lin CF, Hzuka H. (1982) Production of extracelluar pigment by a mutant of Monascus kaoliang sp. nov. Apv. Environ. Microbiol., 43, 671-676
63 Wang SF, Holliwell B, Richimond R, Skoweroneck WR. (1981) The role of superoxide and hydroxyl radicals in the degradation of hyaluronic acid induced by metal ions and ascorbic acid. J. Inorg. Biochem., 14, 127-134   DOI   ScienceOn
64 Eizyro N. (1932) Pigment of Monascus pulpurus Went(Part 1). J. Agric. Chem. Soc. Japan, 8, 1007-1015
65 Kang MR, Kim JY, Hyun YJ, Kim HJ, Yeo HY, Song YD, Lee JH. (2008) The effect of red-yeast-rice supplement on serum lipid profile and glucose control in subjects with impaired fasting glucose or impaired glucose tolerance. Korean J. Nutr., 41, 31-40   과학기술학회마을
66 Kiyoshi I, Yoshio M, Keisuke T, Nobukazu T, Sjpiocjo T, Sjorpi A, Makoto T. (1995) Effect of beni-koji extracts on blood pressure in primary hypertensive volunteers. Jpn. J. Nutr., 53, 263-271   DOI