• Journal of Microbiology
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• 제44권5호
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• pp.530-536
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• 2006

Bacteriophage P4, a satellite phage of coliphage P2, is a very useful experimental tool for the study of viral capsid assembly and cos-cleavage. For an in vitro cos-cleavage reaction study of the P2-P4 system, new shortened and selectable markers containing P4 derivative plasm ids were designed as a substrate molecules. They were constructed by swapping the non-essential segment of P4 DNA for either the kanamycin resistance (kmr) gene or the ampicillin resistance (apr) gene. The size of the genomes of the resulting markers were 82% (P4 ash8 delRI:: kmr) and 79% (P4 ash8 delRI:: apr) of the wild type P4 genome. To determine the lower limit of genome size that could be packaged into the small P4-size bead, these shortened P4 plasmids were converted to phage particles with infection of the helper phage P2. The conversion of plasmid P4 derivatives to bacteriophage particles was verified by the heat stability test and the burst size determination experiment. CsCl buoyant equilibrium density gradient experiments confirmed not only the genome size of the viable phage form of shortened P4 derivatives, but also their packaging into the small P4-size head. P4 ash8 delRI:: apr turned out to be the smallest P4 genome that can be packaged into P4-sized head.

• Near Infrared Analysis
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• 제2권1호
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• pp.43-53
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• 2001

A non-invasive spectroscopic method is presented for the measurement of pulmonary edema. Both early diagnosis and quantitative edema estimates were investigated. The spectroscopic determination of pulmonary edema involved the acquisition of diffuse reflectance spectra in the near-infrared (NIR) region with change in water concentration - water is the main constituent of edema fluid. Pulmonary edema was induced into the excised perfused lungs of seven animals by elevating the hydrostatic pressure. Estimates of edema were ascertained from a partial least squares regression of the measured spectral response. Actual edema was determined from the change (increase) in total lung weight. Estimates in relative lung weight increases due to in vitro edema were made with the near infrared spectra. The results revealed that fluid accumulation produced spectral changes in the O-H and C-H absorptions as well as scattering changes in the spectra. Histology of the lung was used to verify the presence or absence of interstitial and alveolar edema. Results demonstrated that near infrared spectroscopy might provide a new tool for clinical assessment of pulmonary edema.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2313-2318
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• 2003

By means of flexible forming processes in sheet metal manufacturing it is possible to produce parts of complex geometry within short manufacturing time. These procedures are suitable especially for prototyping or adjustment of deformation. Here formative procedures like laser forming are increasingly important, because they make the large-scale-like production of the prototypes with the required materials possible. High accuracy and reproducibility of the products is the precondition of the production. Due to the lack of a forming tool, complex geometries can hardly be manufactured within tolerances. To overcome this problem an automatic closed loop control system for the adjustment of deformations has been developed. An important element of the closed loop control system is the definition of a suitable irradiation strategy for laser forming. For the determination of the irradiation strategy a lot of influences must be taken into consideration from the field of material, geometry and laser. In this paper the improved closed loop control system and the development of an irradiation strategy for 4 mm deep buckles in an ALMgSi1 sheet will be represented. This system can be used e.g. in the automated adjustment of hail damage in car bodies or deformation by heat treatment.

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.1902-1906
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• 2010

A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) ($M_r$ = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power ($R_s$ = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권8호
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• pp.3943-3957
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• 2016

As digital evidence has a highly influential role in proving the innocence of suspects, methods for integrity verification of such digital evidence have become essential in the digital forensic field. Most surveillance camera systems are not equipped with proper built-in integrity protection functions. Because digital forgery techniques are becoming increasingly sophisticated, manually determining whether digital content has been falsified is becoming extremely difficult for investigators. Hence, systematic approaches to forensic integrity verification are essential for ascertaining truth or falsehood. We propose an integrity determination method that utilizes the structure of the video content in a Video Event Data Recorder (VEDR). The proposed method identifies the difference in frame index fields between a forged file and an original file. Experiments conducted using real VEDRs in the market and video files forged by a video editing tool demonstrate that the proposed integrity verification scheme can detect broken integrity in video content.

• Structural Engineering and Mechanics
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• 제73권6호
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• pp.641-649
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• 2020

The determination of midtower longitudinal stiffness has become an essential component in the preliminary design of multi-tower suspension bridges. For a specific multi-tower suspension bridge, the midtower longitudinal stiffness must be controlled within a certain range to meet the requirements of sliding resistance coefficient and deflection-to-span ratio. This study presents a numerical method to divide different types of midtower and determine rational range of longitudinal stiffness for rigid midtower. In this method, influence curves of midtower longitudinal stiffness on sliding resistance coefficient and maximum vertical deflection-to-span ratio are first obtained from the finite element analysis. Then, different types of midtower are divided based on the regression analysis of influence curves. Finally, rational range for longitudinal stiffness of rigid midtower is derived. The Oujiang River North Estuary Bridge which is a three-tower four-span suspension bridge with two main spans of 800m under construction in China is selected as the subject of this study. This will be the first three-tower four-span suspension bridge with steel truss girders and concrete midtower in the world. The proposed method provides an effective and feasible tool for engineers to design midtower of multi-tower suspension bridges.

• 한국유체기계학회 논문집
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• 제16권1호
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• pp.24-31
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• 2013

The motion of living organisms such as birds, fishes, and insects, has been analyzed for the purpose of the design of MAV(Micro Air Vehicle) and NAV(Nano Air Vehicle). In this research, natural motion was considered to be applied to the determination of the geometry and motion of AUV(Autonomous Underwater Vehicle). The flapping motion of a number of hydrofoil shapes in AUV was studied, and at the same time, the optimization of the hydrofoil shape and flapping motion was executed that allow the highest thrust and efficiency. The harmonic motion of plunging and pitching of NACA 4 digit series models, was used for the numerical analysis. The meta model was made by using the kriging method in Optimization method and the experimental points of 49 were extracted for the OA(Orthogonal array) in DOE(Design of experiments). Parametric study using this experimental points was conducted and the results were applied to MGA(Micro Genetic Algorithm). The flow simulation model was validated to be an appropriate tool by comparing with experimental data and the optimized shape and motion of AUV was turned out to produce highest thrust and efficiency.

• 한국환경성돌연변이발암원학회지
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• 제24권3호
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• pp.137-142
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• 2004

Dioxin-like compounds are ubiquitous environmental polltants that could be accumulated in biological system and toxic to human and wildlife. Given this issue, it is important to develop a reliable dioxin detection methods for a rational risk assesment of dioxin-like compounds. In this study, we tried to set up and validate a sensitive, reliable risk assessment of dioxin-like compounds. In this study, we tried to set up and validate a sensitive, reliable and rapid bioassay model, CALUX bioassay as a screening tool for routine measurement of dioxin-like conpounds in environmental matrices. For the valisation of CALUX bioassay, firstly, we performed dose-response assay for 2,3,7,8-TCDD, most potent dioxin-like compound, using two different methods CALUX and EROD assay. Induction of luciferase activity and CYPIA catalyzed EROD activity were dose-dependently induced by 2,3,7,8-TCDD, with initial induction at 0.1 pM and maximal induction at 1 nM. In order t determine whether the CALUX bioassay could predict the effects of dioxin-like compounds, 2,3,7,8-TCDD dose-response from CALUX was compared with that from EROD assay. The correlation coefficient ($r^2$) was found to be 0.89, indicating a good correlation between two different methods and the possibility of CALUX bioassay as a useful dioxin detecting method.

• 대한임상검사과학회지
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• 제40권2호
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• pp.94-97
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• 2008

The human leukocyte antigen (HLA) is the name of the major histocompatibility complex (MCH) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6. and encode cell surface antigen-presenting proteins and many other genes. HLA class I antigen (A, B & C) present peptides from inside the cell. These peptides are produced from digested proteins that are broken down in the lysozymes. Most expressed HLA loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this sutdy, the HLA-A genotypes were determined in twenty students unrelated koreans using the PCR-SSP (Polymerase Chain Reaction-Sequence Specific Primer) technique. Several specific primer pairs in assigning the HLA-A gene were used (A*0201, A*33, A*2401). The results of PCR-SSP, the HLA-A*0201 primer was detected eleven (55%), the HLA-A*33 were detected seven (35%) and the HLA-A*2401 were detected seven (35%). This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-A genotypes.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2000년도 국제심포지움 및 추계학술대회
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• pp.31-41
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• 2000

The major issue in the post genome sequencing era is determination of gene expression patterns in variety of biological systems. A microarray system is a powerful technology for analyzing the expression profile of thousands of genes at one experiment. In this study, we constructed cDNA microarray which carries 2,304 cDNAS derived from oligo-capped mouse cDNA library. Using this hand-made microarray we determined gene expression in various biological systems. To determine tissue specific genes, we compared Nine genes were highly-expressed in adult mouse brain compared to kidney, liver, and skeletal muscle. Tissue distribution analysis using DNA microarray extracted 9 genes that were predominantly expressed in the brain. A database search showed that five of the 9 genes, MBP, SC1, HiAT3, S100 protein-beta, and SNAP25, were previously known to be expressed at high level in the brain and in the nervous system. One gene was highly sequence similar to rat S-Rex-s/human NSP-C, suggesting that the gene is a mouse homologue. The remaining three genes did not match to known genes in the GenBank/EMBL database, indicating that these are novel genes highly-expressed in the brain. Our DNA microarray was also used to detect differentiation specific genes, hormone dependent genes, and transcription-factor-induced genes. We conclude that DNA microarray is an excellent tool for identifying differentially expressed genes.