• The Journal of Engineering Geology
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• v.29 no.4
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• pp.553-563
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• 2019

In order to survey archaeological sites, drilling and excavation are carried out at the final stage. However, at the preliminary stage, non-excavation geophysical prospection is used for assessing underground archaeological ruins. Among the geophysical prospecting techniques, Ground Penetration Radar (GPR) prospection has effectively been applied to historical sites due to its high resolution at shallow depths. In this study, the GPR prospection was conducted to find underground ruins near Suseong-Dang, the place of ancient rituals in Buan area, Korea. First, the GPR prospection was conducted at three sites (Site-1, 2, and 3), and subsequently, the GPR prospection was carried out at Site-3 in more detail. As a result of the prospection, the underground layered structure of the survey area consists of three layers, which are soil layer, weathered rock, and sound rock from the surface. And the GPR anomaly to the archaeological structure was clearly identified at around 100-cm depth showing est-west direction that is parallel to the long-axis array. This GPR anomaly of irregular geomorphological features and intermittent distribution may be related to the ritual remains found in Suseong Dang. The GPR prospection could be effectively used to detect archaeological sites or remains buried in the ground.

• Journal of Life Science
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• v.26 no.5
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• pp.620-631
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• 2016

Viroids are about 250-400 base pair of short single strand RNA fragments have been associated with economically important plant diseases. Due to the lack of protein expression capacity associated with replication, it is very difficult to diagnosis viroid diseases in serological methods. For detecting viroid at plants, molecular-based techniques such as agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), DNA-hybridization, blotting analysis and conventional RT-PCR are reliable. Real-time RT-PCR methods that grafted on RT-PCR methods with improved confirmation methods have been also utilized. However, they are still labor-intensive, time-consuming, and require personnel with expertise. Loop-mediated Isothermal Amplification (LAMP) method is a nucleic acid amplification method under the isothermal condition. The LAMP methodology has been reported to be simple, rapid, sensitive and field applicable in detecting a variety of pathogens. The results of LAMP method can be colorized by adding a visible material such as SYBR green I, Evagreen, Calcein, Berberine and Hydroxy naphthol blue (HNB) with simple equipment or naked eyes. The combination of LAMP method and nucleic pathogens, viroids, can be used to realize simple diagnosis platform for the genetic point-of care testing system. The aim at this review is to summary viroid-caused diseases and the simple visible approach for diagnosing viroids using Loop-mediated Isothermal Amplification (LAMP) method.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.315-324
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• 2004

Cytochrome P450 aromatase is the enzyme responsible for biosynthesis of female sex hormone(estrogen) and 19-nortestosterone(nandrolone), a unique steroid hormone endogenously synthesized in the pig. By use of RT-PCR coupled with DHPLC technique (WAVE analysis), expression pattern of isoforms of porcine cytochrome P450 aromatase gene was investigated. Relatively higher expression of aromatase mRNA was observed in testis than in ovary and this result accounted for the previous findings of higher blood estrogen level in male compared with female in this species. The result from the DHPLC demonstrated that PCR amplified DNA fragments of ovary and testis tissues. using unique PCR primers for all three types of aromatase genes, were different from those of type II and ill genes. Further nucleotide sequence analyses of the plasmid clones containing the PCR products revealed that nucleotide sequences of all clones were identical to type I aromatase gene(ovary type). Thus, the result from the present study indicates that the ovary and testis express the same type of aromatase gene. Therefore, the efficacy of DHPLC techniques used for this study helped us to analyze tissue-specific expression of isoform of genes containing the nucleotide sequences with high homology.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.32 no.5
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• pp.495-503
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• 2014

As the number of vehicles has been sharply increases, the significance of safety and effective operation issues in the parking lot is being emphasized, which takes a part of the transportation system. Recently, there have been several studies for the parking management by detecting moving object, however, recognizing numbers of fast-moving vehicles simultaneously in the picture is still a challenging problem. The parking lot in public area, or large-sized buildings has clear parking section, whereas the sensor system is configured to monitor a plurality of parking spaces. Therefore, by considering those parking lots, we suggested to develop the real-time parking availability information system by applying the real-time image processing techniques. with the help of template matching. Following the study, we wanted to provide the alternative method for parking management system through the reference template makers by recognizing movements of parked vehicles with the size and shape, regardless of direct detecting of driving movements. In addition, we evaluated the applicability and performances of the information system, presented in this study, and implemented a prototype system to simulate the parking statuses of each floor. In fat, it was possible to manage and analyze statistics about the total number of parking spaces and the number of vehicles parked through real-time video flames. We expected that the result of the study will be advanced, following the user-friendliness and cost reduction in operating parking management system and giving information by efficient analysis of parking situation.

• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.2
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• pp.181-185
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• 2010

Purpose: Several studies report that detection of thyroglobulin (Tg) in fine-needle aspiration (FNA) biopsy washout fluid from lymph nodes identifies recurrences or metastases of differentiated papillary thyroid cancer (DPTC) in the neck with higher sensitivity and specificity than fine-needle aspiration cytology (FNAC). We evaluate the diagnostic efficacy and usefulness of Tg measurement in FNA washout fluid (FNA-Tg) and compare with FNAC. Materials and Methods: Forty-eight FNA samples of 37 patients who undergone ultrasonography to detect cervical lymph node metastasis of DPTC, were included for this study. Lymph node metastasis was confirmed by histopathologic examination or long-term imaging follow-up. Sensitivity, specificity and accuracy of FNA-Tg and FNAC were calculated. In 34 patients, we evaluated diagnostic accuracy of FNA-Tg according to the presence or absence of Tg antibody. Results: Sensitivity, specificity and accuracy of FNAC were 75.0%, 97.2% and 91.7%, respectively, and those of FNA-Tg were 100%, 88.9% and 91.7%, respectively. The presence of Tg antibody was not relevant to the diagnostic accuracy of FNA-Tg. Conclusion: FNA-Tg is a as accurate as FNAC with higher sensitivity. FNA-Tg and FNAC are complement techniques for diagnosing lymph node metastasis of DTPC.

• Journal of Korean Society of Transportation
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• v.32 no.4
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• pp.410-420
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• 2014

This research suggests a new tram signal priority model which determines signal timings and tram intersection waiting time using analytical method. This model can calculate the signal timings for Early Green and Green Extension among the active tram signal priority techniques by tram detection time of upstream detector. Moreover, it can determine the tram intersection waiting time that means tram intersection travel time delay from a vantage point of tram travel. Under the active tram signal priority condition, priority phases can bring additional green time from variable green time of non-priority phases. In this study, the signal timing and tram intersection waiting time calculation model was set up using analytical methods. In case studies using an isolated intersection, this study checks tram intersection waiting time ranged 12.7 to 29.4 seconds when variable green times of non-priority phases are 44 to 10 seconds under 120 seconds of cycle length.

• Journal of Conservation Science
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• v.22
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• pp.87-98
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• 2008

Microstructures and chemical compositions of 24 bronze mirrors recovered from the Korean peninsula were examined using the scanning electron microscope equipped with the energy dispersive spectrometer in an effort to characterize the treatments applied on their surface. Their provenance and chronology are mostly unspecified except for two objects from a Koryo burial site. In antiquity the surface of bronze mirrors was frequently finished by mere polishing when their tin content was high enough to guarantee the required reflectivity. In many cases, however, their surface was given a special treatment. The most typical treatment was to coat the surface with tin in two different processes referred to as wipe-tinning and amalgam-tinning. In wipe-tinning only tin was used, but in amalgam-tinning tin and mercury were used together. The surface was often coated with mercury in a process known as mercury-polishing. The present mirrors showed that all these techniques were in fact practiced, not only on the reflective surface but, in some cases, on the decorative surface. The detection of mercury played a crucial role in the assessment of a specific technique applied in each mirror. Mercury often remained in the substrate in the form of sulfide and thereby allowed the method of surface treatment to be estimated even when the coated layer was completely lost. The future study is expected to uncover the regional and temporal variation of the surface treatments to the better understanding of bronze mirrors with respect to provenance and chronology.

• Journal of Environmental Health Sciences
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• v.39 no.5
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• pp.408-417
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• 2013

Objectives: Phthalates are used as plasticizers in polyvinyl chloride (PVC) plastics. As phthalate plasticizers are not chemically bound to the PVC, they can leach, migrate or evaporate into indoor air and atmosphere, foodstuffs, other materials, etc. Therefore, humans are exposed through ingestion, inhalation, and dermal exposure over their entire lifetime, including during intrauterine development. In particular, university students have a great number of opportunities to contact products including phthalates during campus life (food packaging, body care products, cosmetic, lotions, aftershave, perfume etc.). The purpose of this study was to examine levels of phthalate exposure as undergraduate students begin to use pharmaceuticals and personal care products including phthalates. Methods: Phthalate metabolites, mono-ethyl phthalate (MEP), mono-n-butyl phthalate (MnBP), mono-isobutyl phthalate (MiBP), mono-2- ethylhexyl phthalate (MEHP), {(mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP}, and mono-(2-ethlyl-5-oxohexyl) phthalate (MEOHP} were examined. 80 urine samples collected from university students were analyzed using LC/MS/MS(API 4000, Applied Bioscience) with on-line enrichment and columnswitching techniques. This study was carried out at Y university located in Gyonggi Province from 2008 to 2011. Results: The detection limit of phthalate metabolites were 0.03 ng/mL for MEP, 0.11 ng/mL for MnBP, 0.08 ng/mL for MiBP, 0.93 ng/mL for MEHP, 0.19 ng/mL for MEOHP and 0.16ng/mL for MEHHP. MnBP showed the highest urinary levels (median: 31.6 ug/L, 24.8 ug/g creatinine (cr)). Concentrations were also high for MEHHP (median: 24.1 ug/L, 19.0 ug/g cr), followed by MEOHP (median: 22.8 ug/L, 17.9 ug/g cr). In individual cases, the maximum level reached up to 348 ug/L, and 291 ug/g cr, respectively. The urinary and creatinine adjusted levels of MEP were lower than those for DBP and DEHP metabolites, but were higher in 95th percentiles. As a result, the mean daily DEP intake value was 2.3 ${\mu}g/kg$ bw/day, 3.5 ${\mu}g/kg$ bw/day for DEHP and 4.9 ${\mu}g/kg$ bw/day for DBP. Conclusion: These students' phthalate exposure levels were below the international safe level set by the EU, but higher than the 2012 KFDA survey of the age group from 3 to 18.

• Progress in Medical Physics
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• v.14 no.3
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• pp.151-160
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• 2003

The application of more complex radiotherapy techniques using multileaf collimation (MLC), such as 3D conformal radiation therapy and intensity-modulated radiation therapy (IMRT), has increased the significance of verifying leaf position and motion. Due to thier reliability and empirical robustness, quality assurance (QA) of MLC. However easy use and the ability to provide digital data of electronic portal imaging devices (EPIDs) have attracted attention to portal films as an alternatives to films for routine qualify assurance, despite concerns about their clinical feasibility, efficacy, and the cost to benefit ratio. In this study, we developed method for daily QA of MLC using electronic portal images (EPIs). EPID availability for routine QA was verified by comparing of the portal films, which were simultaneously obtained when radiation was delivered and known prescription input to MLC controller. Specially designed two-test patterns of dynamic MLC were applied for image acquisition. Quantitative off-line analysis using an edge detection algorithm enhanced the verification procedure as well as on-line qualitative visual assessment. In conclusion, the availability of EPI was enough for daily QA of MLC leaf position with the accuracy of portal films.

• Analytical Science and Technology
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• v.24 no.5
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• pp.352-359
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• 2011

An analytical method for solvent-free determination of benzene, toluene, ethylbenzene, and xylenes (BTEX) in water using repetitive membrane extractions coupled to cryofocusing and GC-MS was derived. BTEX compounds that permeated through a nonporous silicone membrane from the aqueous phase and evaporated into the acceptor phase were purged into a cryofocusing trap ($-100^{\circ}C$) with helium gas. The BTEX compounds, thus enriched in the trap, were thermally desorbed into a capillary column GC and detected using an MS. The flow rate of the donor phase (30 mL water) was set at 10 mL/min, and membrane extractions, accomplished by returning the water drained from the extraction module to the sample container, were repeated three times at $20{\pm}2^{\circ}C$. Although recoveries (%) were variable, from the highest for benzene (approximately 80%) to the lowest for ethylbenzene and xylenes (3.5-10%), the method showed satisfactory precision (RSD 2.2-10%) with good-linearity calibration curves ($r^2$ 0.9976-0.9997 in 1-100 ${\mu}g$/L range) for all of the compounds. The method detection limits (MDLs) ranged from 0.16 to 1.8 ${\mu}g$/L. The results showed the method's advantages such as short analysis time and overall simplicity without solvent compared to the conventional techniques.