• 대한수의학회지
• /
• 제37권2호
• /
• pp.349-358
• /
• 1997

Mycobacterium paratuberculosis is the etiologic agent of Johne's disease, a chronic inflammatory bowel syndrome in ruminants. The attempts to control or eradicate the disease were severely hampered by the inadequacies of present diagnostic methods. The first purpose of this study was to detect Johne's disease out of 577 cows in the province of Kyunggi, Chungchong, Gangweon and the second purpose was to compare the results of non-absorbed ELISA, absorbed ELISA, PCR, and conventional culture methods. The third purpose was to increase diagnostic specificity, accuracy and rapidity. When non-absorbed ELISA test was conducted with Mycobacterium paratuberculosis antigen, the prevalence of positive was 10.9%. To increase diagnostic specificity, absorbed ELISA test with Mycobacterium phlei was used. In this test, the positive prevalence was 1.7%. For the specific detection of Mycobacterium paratuberculosis, PCR was applied to bacterial culture obtained from fecal samples of cattle. The DNA sequences derived from IS900 were used to prepare DNA primers for detection and identification of Mycobacterium paratuberculosis by PCR. PCR for M paratuberculosis isolated from fecal cultures amplified specific target DNA. PCR was much more rapid than that obtained by conventional culture technique in diagnosis of Johne's disease.

• 대한수의학회지
• /
• 제38권4호
• /
• pp.763-770
• /
• 1998

Salmonella typhimurium is a causitive agent of diarrhea, fever, gastroenteritis, septicemia and sudden death in piglet. The currently used methods such as IFA, ELISA, DNA hybridization assay is needed a long-time and difficult to detect the organism in carrier animal or contaminated sample with other agents. However, it is important to detect rapidly and sensitively S typhimurium in piglet with other infectious pathogens to minimize an economic loss. Two sets of PCR primer, rfbJ forward primer(5'-AGAATATGTAATTGTCAG-3') and reverse primer(5'-TAACCGTTTCAGTAGTTC-3') were designed to amplify a 882 by fragment of Salmonella serovar type B gene. The target genomic DNA for PCR was extracted from the cultivated materials with various enrichment periods in a nonselective enrichment agar and broth with clinical specimens. The PCR is carried out here made it possible to detect the gene from two hours. Also, the amplified fragment with PCR was cloned into pGEM-T vector and digested with restrict enzyme, and sequenced for the identification of Salmonella serotype B rfbJ gene. Duplicated cultivation agar-broth followed by PCR were performed to develop a rapid and sensitive detection of S typhlmurium based on serovar type. This duplicated cultivation-PCR method provides a sensitive and rapid diagnostic tool to detect Salmonella from infected piglet with improved sensitivity.

• Bulletin of the Korean Chemical Society
• /
• 제31권7호
• /
• pp.1907-1914
• /
• 2010

A novel catalytic kinetic method is proposed for the determination of Se(IV), Se(VI) and total inorganic selenium in water based on the catalytic effect of Se(IV) on the reduction of bromate by p-nitrophenylhydrazine at pH 3.0. The generated bromine, $Br_2$ or $Cl_2$ plus $Br_2$ in 0.1 M NaCl (or NaBr) environment efficiently decolorized Calmagite and the reaction was monitored spectrophotometrically at 523 nm as a function of time. In this indicator reaction, bromide acted as an activator for the catalysis of selenium (IV) and a reducing agent for selenium (VI) at pH 3.0, which allowed the determination of total selenium. The fixed time method was adopted for the determination and speciation of inorganic selenium. Under the optimum conditions, the calibration graph are linear in the range 1 - 35 ${\mu}gL^{-1}$ of Se(IV) for the fixed time method at $25^{\circ}C$. The detection limit based on statistical $3S_{blank}$/m-criterion was 0.215 ${\mu}gL^{-1}$ for the fixed time method (7 min). All of the variables that affect the sensitivity at 523 nm were investigated, and the optimum conditions were established. The interference effect of various cations and anions on the Se (IV) determination was also studied. The selectivity of the selenium determination was greatly improved with the use of the strongly cation exchange resin such as Amberlite IR120 plus. The proposed kinetic method was validated statistically and through recovery studies in natural water samples. The RSDs for ten replicate measurements of 5, 15 and 25 ${\mu}gL^{-1}$ of Se(IV) and Se(VI) was changed between 2.1 - 4.85%. Analyses of a certified standard reference material (NIST SRM 1643e) for selenium using the fixed-time method showed that the proposed kinetic method has good accuracy. Se(IV), Se(VI) and total inorganic selenium in environmental water samples have been successfully determined by this method after selective reduction of Se(VI) to Se(IV).

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권6호
• /
• pp.3963-3967
• /
• 2013

Background: The presence of Epstein-Barr virus (EBV) in Non-Hodgkin's lymphoma can be identified by immunohistochemistry for detection of EBV latent membrane protein (LMP). The role of EBV as an etiologic agent in the development of non-Hodgkin lymphoma has been supported by detection of high levels of latent membrane protein 1 (LMP-1) expression in tumors. However, no study has been conducted in a Pakistani population up till now to determine the frequency of Epstein-Barr virus positivity. The objective of our study was to determine a value for non-Hodgkin lymphoma patients using EBV LMP-1 immunostaining in our institution. Materials and Methods: This study was carried out at the Department of Histopathology, Armed Forces Institute of Pathology (AFIP), Pakistan from December 2011 to December 2012. It was a cross sectional study. A total of 71 patients who were diagnosed with various subtypes of NHL after histological and EBV LMP-1 immunohistochemical evaluation were studied. Sampling technique was non-probability purposive. Statistical analysis was achieved using SPSS version 17.0. Mean and SD were calculated for quantitative variables like patient age. Frequencies and percentages were calculated for qualitative variables like subgroup of NHL, results outcome of IHC for EBV and gender distribution. Results: Mean age of the patients was $53.6{\pm}16$ years (Mean${\pm}$SD). A total of 50 (70.4%) were male and 21 (29.6%) were female. Some 9 (12.7%) out of 71 cases were positive for EBV-LMP-1 immunostaining, 2 (22.2%) follicular lymphoma cases, 1 (11.1%) case of T-cell lymphoblastic lymphoma, 4 (44.4%) cases of diffuse large B cell lymphomas, 1 (11.1%) mantle cell lymphoma and 1 (11.1%) angioimmunoblastic T cell lymphoma case. Conclusion: In our study, frequency of EBV in NHL is 12.7% and is mostly seen in diffuse large B cell lymphoma. This requires further evaluation to find out whether this positivity is due to co-infection or has a role in pathogenesis.

• 상하수도학회지
• /
• 제28권2호
• /
• pp.171-179
• /
• 2014

In this study, a novel method was proposed to test the integrity of water treatment system specifically equipped with membrane filtration process. We applied the silica particles coated with a fluorescent agent (rhodamine B isothiocyanate) as a surrogate to detect a membrane process integrity and evaluate the reliability of effluent quality in the system. Additionally, a series of experiments was conducted to evaluate the sensitivity of the method through the laboratory scale experiment. The laboratory scale experiments showed that the feasibility of application of proposed method to detect a breach or damaged part on the membrane surface. However, the sensitivity on predicting the area of a breach was significantly influenced by the testing conditions such as a concentration of surrogate, filtration flux, and detection time. The lowest error of predicting the area of breach was 3.5% at the testing condition of surrogate concentration of 80 mg/L injected with flux of $20L/m^2/hr$ for 10 minutes of detection time for the breach having the actual area of $7.069mm^2$. However, the error of estimation was increased at the small breach with area less than $0.785mm^2$. A future study will be conducted to estimate a damaged area with more accuracy and precision.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2007년도 한국컴퓨터종합학술대회논문집 Vol.34 No.1 (C)
• /
• pp.332-335
• /
• 2007

이메일은 누구나 쉽게 정보를 교환할 수 있는 편리함 때문에 인터넷에서 가장 중요한 수단으로 사용되고 있다. 그러나 순수한 의사소통의 수단이 아닌 스팸메일의 범람은 성인뿐만 아니라, 어린이 청소년에게도 무차별적으로 전송됨으로써 심각한 부작용을 낳고 있다. 본 논문은 점차 지능화 되는 신 유형의 음란 스팸메일로부터 청소년을 보호하기 위하여 새로운 방법의 음란메일 차단시스템을 제안하고자 한다. 기존의 스팸메일 차단시스템은 사용자가 직접 음란한 메일이라고 판단되는 메일에 대해 일일이 키워드를 설정하거나, 메일 내용 중에 텍스트만을 추출하여 패턴 매칭방법으로 분류하는 것이 대부분이었지만, 본 논문은 기존 방법의 문제점을 해결하기 위하여 이미지 내 Skin-Color분포의 Human Detection 알고리즘과 웹 로봇 에이전트의 하이퍼링크 분석기법을 사용하였다. 성능 측정결과, 형태소 분석과 Human Detection 알고리즘을 병합하여 적용한 경우 성능 측정에서 90% 정도의 F-measure를 보였지만, 추가적으로 웹 로봇 에이전트의 하이퍼링크 분석기법을 병합하여 적용한 경우 97% 이상의 F-measure를 보이며, 신뢰성이 높은 음란스팸메일 차단 시스템을 구현할 수 있다는 것을 증명하였다.

• 한국정보과학회논문지:정보통신
• /
• 제35권4호
• /
• pp.301-310
• /
• 2008

기존의 MIPv6는 오랜 시간동안의 핸드오버로 인하여 많은 패킷 손실 및 오랜 세션 단절을 야기시킨다. 이러한 문제점들을 향상시키기 위하여 Fast handover for Mobile IPv6(FMIPv6) 프로토콜이 개발되었지만 여전히 터널링에 기반한 라우팅 방법은 패킷 순서 어긋남 문제로 인하여 성능이 하락하는 문제를 야기한다. 최근 모바일 단말에서의 이동성 관리 부하를 줄여주기 위하여, 네트워크 이동성 기반인 Proxy Mobile IPv6(PMIPv6)가 제안되었다. PMIPv6는 모바일 단말에서 수행하던 이동성 관리를 네트워크 에이전트에서 해줌으로서 단말의 부하를 줄이고 이동성 관리 지연 시간을 줄일 수 있다. 본 논문에서는 PMIPv6에서 안정되고 향상된 최적화 라우팅 기술이 접목된 빠른 핸드오버 방법인 Fast Proxy Mobile IPv6(EF-PMIPv6) 제안한다. 제안한 EF-PMIPv6는 고속의 IAPP 기술 및 ND 기법을 이용하여 기존의 MIPv6와 PMIPv6 기법에 비하여 고속의 핸드오버를 지원해 줄 수 있다. 또한 수식분석을 통하여 제안한 방법의 향상된 점을 보여준다. 분석에 있어서는 다양한 파라미터들을 이용하여 제안한 방법과 기존의 방법들에 있어서 핸드오버 지연시간을 비교하였다.

• The Plant Pathology Journal
• /
• 제36권5호
• /
• pp.418-427
• /
• 2020

Xanthomonas campestris pv. campestris (Xcc), the pathogen of black rot which is the most destructive disease of Brassica vegetables throughout the world. Here, we reported two novel sequence-characterized amplified region (SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of Xcc race 6 via re-alignment of the complete genome sequences of Xcc races/strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of Xcc races/strains/pathovars and two other plant infecting bacterial strains. The PCR result revealed that the XccR6-60 and XccR6-67 primer sets amplified 692-bp and 917-bp DNA fragments, respectively, specifically from race 6, while no visible amplification was detected in other samples. In addition, the SCAR primers were highly sensitive and can detect from a very low concentration of genomic DNA of Xcc race 6. However, the complete genome sequence of Xcc race 6 is not yet publicly available. Therefore, the cloning and sequencing of XccR6-60 and XccR6-67 fragments from race 6 provide more evidence of the specificity of these markers. These results indicated that the newly developed SCAR markers can successfully, effectively and rapidly detect Xcc race 6 from other Xcc races/strains/pathovars as well as other plant pathogenic bacteria. This is the first report for race-specific molecular markers for Xcc race 6.

• Journal of Microbiology and Biotechnology
• /
• 제27권1호
• /
• pp.130-140
• /
• 2017

It is vital to understand the changing characteristics of interphase microbial communities and interspecies synergism during the fermentation of Chinese liquors. In this study, microbial communities in the three indispensable phases (pit mud, zaopei, and huangshui) of Luzhou-flavored liquor manufacturing pits and their shifts during cellars use were first investigated by polyphasic culture-independent approaches. The archaeal and eubacterial communities in the three phases were quantitatively assessed by combined phospholipid ether lipids/phospholipid fatty acid analysis and fluorescence in situ hybridization. In addition, qualitative information regarding the microbial community was analyzed by PCR-denaturing gradient gel electrophoresis. Results suggested that the interphase microbial community profiles were quite different, and the proportions of specific microbial groups evolved gradually. Anaerobic bacteria and gram-positive bacteria were dominant and their numbers were higher in pit mud ($10^9$ cells/g) than in huangshui ($10^7$ cells/ml) and zaopei ($10^7$ cells/g). Hydrogenotrophic methanogenic archaea were the dominant archaea, and their proportions were virtually unchanged in pit mud (around 65%), whereas they first increased and then decreased in zaopei (59%-82%-47%) and increased with pit age in huangshui (82%-92%). Interactions between microbial communities, especially between eubacteria and methanogens, played a key role in the formation of favorable niches for liquor fermentation. Furthermore, daqu (an essential saccharifying and fermentative agent) and metabolic regulation parameters greatly affected the microbial community.

• 대한화장품학회지
• /
• 제30권4호
• /
• pp.479-483
• /
• 2004

MITF는 미백관련 유전자의 대표적인 조절 인자 단백질로서 미백관련 유전자의 E-box와의 결합정도를 단백질 칩을 이용하여 측정하였다. 융합 단백질 형태의 MITF를 유리 칩에 고정시켰고 E-box를 포함하는 DNA oligomer가 결합하는 것을 확인하였다. 형광법, SPR (surface plasmon resonance), SPRi (surface plasmon resonance imaging)방법 중 형광법이 가장 효과적이었으며, DNA 저해제를 사용시 결합이 감소하는 것을 확인하였다. 이 결과 MITF를 이용한 미백원료의 고속스크리닝(HTS)의 가능성을 보여주었다.