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Optimization of Ethanol Extraction of $\gamma$-oryzanol and Other Functional Components from Rice Bran (미강의 $\gamma$-oryzanol 및 생리활성물질의 에탄올 추출공정 최적화)

  • Jo, In-Hee;Choi, Yong-Hee
    • Food Science and Preservation
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    • v.17 no.2
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    • pp.281-289
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    • 2010
  • We determined the optimum ethanolic conditions for extraction of $\gamma$-oryzanol and other functional components from rice bran, using response surface methodology (RSM). A central composite design was used to investigate the effects of the independent variables of solvent ratio ($X_1$), extraction temperature ($X_2$), and extraction time ($X_3$), on dependent variables including yield ($Y_1$), total phenolic content ($Y_2$), electron-donating activity ($Y_3$), ferulic acid level ($Y_4$), and $\gamma$-oryzanol concentration ($Y_5$). Solvent ratio and extraction temperature were the most important factors in extraction. The maximum yield was at 22.56 mL/g ($X_1$), 78.19C ($X_2$), and 522.15 min ($X_3$), at the saddle point. Total phenolic levels were little affected by solvent ratio or extraction temperature. The maximum concentration of extracted total phenolics was 90.78mg GAE/100 g at 21.26 mL/g, $94.65^{\circ}C$, and 567.97 min. A maximum electron-donating ability of 54.72% was obtained with the parameters 20.20 mL/g,$81.89^{\circ}C$, and 701.87 min, at the highest point. The maximum level of ferulic acid components was 210.47 mg/100g at 5.22 mL/g, $79.66^{\circ}C$, and 575.24 min. In addition, the maximum $\gamma$-oryzanol concentration was 660.39 mg/100g at 5.10 mL/g, $81.83^{\circ}C$, and 587.39 min. The optimum extraction conditions were a solvent ratio of 10.45 mL/g, $80^{\circ}C$ extraction temperature, and 535 min extraction time. Predicted extraction levels under optimized conditions were in line with experimental values.

Site Characterization using Shear-Wave Velocities Inverted from Rayleigh-Wave Dispersion in Chuncheon, Korea (레일리파 분산을 역산하여 구한 횡파속도를 이용한 춘천시의 부지특성)

  • Jung, JinHoon;Kim, Ki Young
    • Geophysics and Geophysical Exploration
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    • v.17 no.1
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    • pp.1-10
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    • 2014
  • To reveal and classify site characteristics in densely populated areas in Chuncheon, Korea, Rayleigh-waves were recorded at 50 sites including four sites in the forest area using four 1-Hz velocity sensors and 24 4.5-Hz vertical geophones during the period of January 2011 to May 2013. Dispersion curves of the Rayleigh waves obtained by the extended spatial autocorrelation method were inverted to derive shear-wave velocity ($v_s$) models comprising 40 horizontal layers of 1-m thickness. Depths to weathered rocks ($D_b$), shear wave velocities of these basement rocks ($v_s^b$), average velocities of the overburden layer ($\bar{v}_s^s$), and the average velocity to a depth of 30 m ($v_s30$), were then derived from those models. The estimated values of $D_b$, $v_s^b$, $\bar{v}_s^s$, and $v_s30$ for 46 sites at lower altitudes were in the ranges of 5 to 29 m, 404 to 561 m/s, 208 to 375 ms/s, and 226 to 583 m/s, respectively. According to the Korean building code for seismic design, the estimated $v_s30$ indicates that the lower altitude areas in Chuncheon are classified as $S_C$ (very dense soil and soft rock) or $S_D$ (stiff soil). To determine adequate proxies for $v_s30$, we compared the computed values with land cover, lithology, topographic slope, and surface elevation at each of the measurement sites. Due to a weak correlation (r = 0.41) between $v_s30$ and elevation, the best proxy of them, applications of this proxy to Chuncheon of a relatively small area seem to be limited.

A Study on Laboratory Treatment of Metalworking Wastewater Using Ultrafiltration Membrane System and Its Field Application (한외여과막시스템을 이용한 금속가공폐수의 실험실적 처리 및 현장 적용 연구)

  • Bae, Jae Heum;Hwang, In-Gook;Jeon, Sung Duk
    • Korean Chemical Engineering Research
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    • v.43 no.4
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    • pp.487-494
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    • 2005
  • Nowadays a large amount of wastewater containing metal working fluids and cleaning agents is generated during the cleaning process of parts working in various industries of automobile, machine and metal, and electronics etc. In this study, aqueous or semi-aqueous cleaning wastewater contaminated with soluble or nonsoluble oils was treated using ultrafiltration system. And the membrane permeability flux and performance of oil-water separation (or COD removal efficiency) of the ultrafiltration system employing PAN as its membrane material were measured at various operating conditions with change of membrane pore sizes and soil concentrations of wastewater and examined their suitability for wastewater treatment contaminated with soluble or insoluble oil. As a result, in case of wastewater contaminated with soluble oil and aqueous or semi-aqueous cleaning agent, the membrane permeability increased rapidly even though COD removal efficiency was almost constant as 90 or 95% as the membrane pore size increased from 10 kDa to 100 kDa. However, in case of the wastewater contaminated with nonsoluble oil and aqueous or semi-aqueous cleaning agent, as the membrane pore size increased from 10 kDa to 100 kDa and the soil concentration of wastewater increased, the membrane permeability was reduced rapidly while COD removal efficiency was almost constant. These phenomena explain that since the membrane material is hydrophilic PAN material, it blocks nonsoluble oil and reduces membrane permeability. Thus, it can be concluded that the aqueous or semi-aqueous cleaning solution contaminated with soluble oil can be treated by ultrafiltration system with the membrane of PAN material and its pore size of 100 kDa. Based on these basic experimental results, a pilot plant facility of ultrafiltration system with PAN material and 100 kDa pore size was designed, installed and operated in order to treat and recycle alkaline cleaning solution contaminated with deep drawing oil. As a result of its field application, the ultrafiltration system was able to separate aqueous cleaning solution and soluble oil effectively, and recycle them. Further more, it can increase life span of aqueous cleaning solution 12 times compared with the previous process.

Grain-Size Trend Analysis for Identifying Net Sediment Transport Pathways: Potentials and Limitations (퇴적물 이동경로 식별을 위한 입도경향 분석법의 가능성과 한계)

  • Kim, Sung-Hwan;Rhew, Ho-Sahng;Yu, Keun-Bae
    • Journal of the Korean Geographical Society
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    • v.42 no.4
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    • pp.469-487
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    • 2007
  • Grain-Size Trend Analysis is the methodology to identify net sediment transport pathways, based on the assumption that the movement of sediment from the source to deposit leaves the identifiable spatial pattern of mean, sorting, and skewness of grain size. It can easily be implemented with low cost, so it has great potentials to contribute to geomorphological research, whereas it can also be used inadequately without recognition of its limitations. This research aims to compare three established methods of grain-size trend analysis to search for the adequate way of application, and also suggest the research tasks needed in improving this methodology 1D pathway method can corporate the field experience into analyzing the pathway, provide the useful information of depositional environments through X-distribution, and identify the long-term trend effectively. However, it has disadvantage of the dependence on subjective interpretation, and a relatively coarse temporal scale. Gao-Collins's 2D transport vector method has the objective procedure, has the capability to visualize the transport pattern in 2D format, and to identify the pattern at a finer temporal scale, whereas characteristic distance and semiquantitative filtering are controversial. Le Roux's alternative 2D transport vector method has two improvement of Gao-Collins's in that it expands the empirical rules, considers the gradient of each parameters as well as the order, and has the ability to identify the pattern at a finer temporal scale, while the basic concepts are arbitrary and complicated. The application of grain sire trend analysis requires the selection of adequate method and the design of proper sampling scheme, based on the field knowledge of researcher, the temporal scale of sediment transport pattern targeted, and information needed. Besides, the relationship between the depth of sample and representative temporal scale should be systematically investigated in improving this methodology.

Spatial Characteristics of Cultural Economy Emergence and Diffusion: Creative Retail Shops in Bukchon (문화경제의 발현과 확산의 공간적 특징: 북촌의 창의적 소매업을 중심으로)

  • Lee, Kyung-Ok;Lee, Keum-Sook
    • Journal of the Economic Geographical Society of Korea
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    • v.9 no.1
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    • pp.23-38
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    • 2006
  • This study attempts to investigate the spatial characteristics of cultural economies of Bukchon in Seoul, where abundant cultural assets exist. This area has been the administrative-political core locale since 14C, therefore, a lot of traditional- and contemporary-cultural facilities are remains, This area possessed unique place characteristics by coexisting traditional and modern cultures, and thus attracted to culture demanders. Recently many economic activities have increased in this area, and most of them are related with the cultural elements of the region, In particular creative retail shops, which produce goods by flexible-specialized production system, have been agglomerated in this area, The goods are designed, produced, and sold at the shop place. Most of them are uniquely designed hand made products, and produced a few amount. The economic value of these products related with the cultural environment of Bukchon. These creative shops emerged at the front of Samcheongdong street in the beginning, and then have diffused into the inside of Samcheongdong street and Hwagae street. The shops have very unique exteriors related with traditional houses in this area, and located on roadsides where are not many passerby. Most owners of the retail stores are highly educated and studied design, and produce goods by themselves at the shops. The reason that they opened their shops in Bukchon is directly related with the unique cultural environment of this area. They hope that more unique and artistic stores join this area so that developed this area as culture and fashion combined area.

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The Effects of the Breast Self-Examination Participant Education program on Breast Self-Examination Compliance and Health Promoting Behaviors in Adult Women (유방 자가 검진 참여 교육 프로그램이 유방 자가 검진 이행과 건강 증진 행위에 미치는 영향)

  • Park, Eun-Hwa;Ryu, Eun-Jung;Choi, Kyung-Sook
    • Asian Oncology Nursing
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    • v.3 no.2
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    • pp.133-144
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    • 2003
  • This study is carrying out a before and after experiment design for the non-equal comparative group to identify the effects of the breast self-examination education on breast self-examination participant education compliance and health promotion in women. The subject of this study was 58 women residing in Chungju. Their age ranged from 20 to 40. These women were not pregnant or did not breast feed, as well as they did not have any breast disease, at the time of survey. They were available for the response to the questionnaires, and understood the purpose of this study. They also agreed to participate in the study, and responded to the 3rd time questionnaires to the end. Thus, brochures and lectures were provided side by side to a group of 19 of those women, and only brochures were provided to another group of 39 of those women. With regard to education, a brochure and a program using a breast model were applied. Then, the frequency of the breast self examination compliance, breast self examination capability and the relations between the breast self examination and activities to promote health were measured, before the education, after 4 weeks of education and after 12 weeks of education. Before education and after 4 weeks of education. I collected the questionnaires myself visiting them, and after 12 weeks of education, the questionnaires were collected by mail. In relation to the study tool, the breast self examination activity was measured by two measuring tools: breast self examination activity frequency and breast self examination compliance capability. As for the frequency, the number of self examination for the period of 3 months, before the questionnaire survey, was measured in the form of self report. In relation to the tool to measure the breast self examination capability, the BSEPRI tool, which was developed by Wood in 1994, was used. Here, as the score was higher, the capability was indicated to be higher. The translated and revised version of Health Promoting Lifestyle Profile II (Walker, Sechrist & Pender. 2002) was used as a tool for health promotion. As the measured score was higher, the health promoting activity was indicated to be higher. The results of this study were as follows: 1. As a result of checking the breast self examination compliance frequency between the experiment group that received the breast self examination participant education and the comparative group that did not receive the education, there was a significant difference in interaction between groups by time, as time passed by. 2. As a result of checking the breast self examination compliance capability between the experiment group and the comparative group, there was a significant difference in interaction between groups by time, as time passed by. 3. As a result of carrying out a repetitive measurement analysis, between the experiment group that received the breast self examination education and the comparative group that did not receive the education, which was carried out to validate the hypothesis that the former would have higher health promoting activities than the latter, there was no significant difference after the breast self examination education was conducted.

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Effects of Protein and Carbohydrate Supplements on Feed Digestion in Indigenous Malaysian Goats and Sheep

  • Darlis, N. Abdullah;Halim, R.A.;Jalaludin, S.;Ho, Y.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.4
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    • pp.464-469
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    • 2000
  • Experiments were conducted to determine the effects of soybean meal (SBM) as a source of protein and sago meal (SM) as a source of carbohydrate on in situ and in vivo digestibility of dietary components in four male goats (Kambing Katjang) and four male sheep (Malin) weighing 25-35 kg. Rumen volume, as well as rumen fluid dilution rate were also determined. The animals were housed in single pens with individual feeding and drinking troughs and each animal was fitted with a rumen fistula. They were fed two diets : chopped rice straw+200 g soybean meal (SBM), and chopped rice straw+190 g soybean meal+300 g sago meal (SBM+SM). Rice straw was offered ad libitum. The supplements were isonitrogenous (80 g crude protein/animal/d), but the proportions of dry matter (DM), organic matter (OM), crude fibre (CF), neutral detergent fibre (NDF) and acid detergent fibre (ADF) were lower in the SBM supplement (191, 165, 11, 40, 15 g/animal/d for DM, OM, CF, NDF and ADF, respectively) than in the SBM+SM supplement (445, 423, 25, 102, 38 g/animal/d for DM, OM, CF, NDF and ADF, respectively). Two animals from each species were fed either supplement in a cross-over design in two periods. Each period lasted for four weeks. In situ and in vivo digestibility studies were carried out, followed by the determination of rumen volume and rumen fluid dilution rate. The results showed that straw DM and total DM intakes of goats (average of $48.7g/kg\;W^{0.75}$, $72.7g/kg\;W^{0.75}$, respectively) were significantly (p<0.01) higher than sheep (average of $3.56g/kg\;W^{0.75}$, $61.6g/kg\;W^{0.75}$, respectively), but OM, N and GE intakes were not significantly different between the two animal species. When the effect of supplements was compared, animals fed SBM+SM supplement had significantly (p<0.001) higher DM, OM and GE intakes than animals fed SBM supplement. Potential degradabilities of rice straw DM were significantly (p<0.01) higher in goats (average of 48.8%) than in sheep (average of 46.1 %). The supplements had no significant effect on the potential degradabilities of DM, OM and NDF, but they had a significant (p<0.05) effect on the degradation rates of DM and NDF. The addition of sago meal in the diet reduced the degradation rates of DM and NDF of rice straw in the rumen. Potential degradability of DM of soybean meal was not significantly different between animal species as well as between supplements. Sago meal was highly degradable. At 24 h of incubation in the rumen, 90-95% of DM loss was observed. There was a significant interaction between animal species and supplements in the in vivo digestibility of ADF and GE. In animals fed SBM supplement, the in vivo digestibility of ADF was significantly (p<0.05) higher in goats ($50.6{\pm}4.22%$) than in sheep ($44.4{\pm}3.21%$), but digestibility of GE was significantly (p<0.05) higher in sheep ($70.2{\pm}1.93%$) than in goats ($63.0{\pm}3.07%$). The digestibility values of CP and OM were significantly (p<0.05) higher in sheep when compared to goats. Animals fed SBM+SM supplement showed significantly (p<0.05) higher DM and OM digestibility values than animals fed SBM supplement, but digestibility values of CP were significantly (p<0.05) higher in animals fed SBM supplement. Differences in in vivo digestibility values of CF and NDF were not significantly different between animal species or supplements. Water intake, rumen volume ($1/kg\;W^{0.75}$), rumen fluid dilution rate and mean retention time were similar between the two animal species. However, rumen fluid dilution rate and mean retention time was significantly (p<0.01) affected by supplements. Animals fed SBM+SM had faster rumen fluid dilution rate and consequently shorter mean retention time.

Factors Influencing the Regular Oral Check-Ups: Based on the Data of the 2014 Korea National Health and Nutrition Examination Survey (우리나라 경제활동자의 구강검진 수진 영향요인: 국민건강영양조사 제6기 2차년도(2014) 자료를 바탕으로)

  • Kim, Dong-Hwi;Seo, Young-Joon
    • Journal of dental hygiene science
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    • v.17 no.4
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    • pp.323-332
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    • 2017
  • This study aimed to investigate the factors influencing the decision on whether to receive regular oral check-ups among the national health insurance beneficiaries with income and aged over 20 years in Korea. This was a cross-sectional study, using the data from the 2014 6th Korea National Health and Nutrition Examination Survey (KNHANES VI-2), conducted by the Korea Centers for Disease Control and Prevention. The final sample included 2,843 subjects who participated in the health examination survey and oral check-ups. The IBM SPSS Statistics ver. 20.0 was used for the statistical analysis, based on the complex sampling design. The Rao-Scott chi-square test was used to verify the difference in the distribution of independent variables with regard to oral check-ups. The binary logistic regression analysis was used to determine the influencing factors. The ratio of beneficiaries who received oral check-ups was less than half of those who received medical examinations. The Rao-Scott chi-square test, revealed significant differences in the age group (p<0.05) of the predisposing factors, toothache within a year (p<0.001), untreated dental caries (p<0.001) of the need factors, personal income level (p<0.001), educational level (p<0.001), type of medical insurance (p<0.001), and private health insurance (p<0.01) of the enabling factors. In the binary logistic regression analysis, factors influencing oral check-ups were age group, personal income level, educational level, toothache within a year, and untreated dental caries. These findings show a variety of factors influencing the utilization of regular oral check-ups.

A Knowledge-based Approach for the Estimation of Effective Sampling Station Frequencies in Benthic Ecological Assessments (지식기반적 방법을 활용한 저서생태계 평가의 유효 조사정점 개수 산정)

  • Yoo, Jae-Won;Kim, Chang-Soo;Jung, Hoe-In;Lee, Yong-Woo;Lee, Man-Woo;Lee, Chang-Gun;Jin, Sung-Ju;Maeng, Jun-Ho;Hong, Jae-Sang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.16 no.3
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    • pp.147-154
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    • 2011
  • Decision making in Environmental Impact Assessment (EIA) and Consultation on the Coastal Area Utilization (CCAU) is footing on the survey reports, thus requires concrete and accurate information on the natural habitats. In spite of the importance of reporting the ecological quality and status of habitats, the accumulated knowledge and recent techniques in ecology such as the use of investigated cases and indicators/indices have not been utilized in evaluation processes. Even the EIA report does not contain sufficient information required in a decision making process for conservation and development. In addition, for CCAU, sampling efforts were so limited that only two or a few stations were set in most study cases. This hampers transferring key ecological information to both specialist review and decision making processes. Hence, setting the effective number of sampling stations can be said as a prior step for better assessment. We introduced a few statistical techniques to determine the number of sampling stations in macrobenthos surveys. However, the application of the techniques requires a preliminary study that cannot be performed under the current assessment frame. An analysis of the spatial configuration of sampling stations from 19 previous studies was carried out as an alternative approach, based on the assumption that those configurations reported in scientific journal contribute to successful understanding of the ecological phenomena. The distance between stations and number of sampling stations in a $4{\times}4$ km unit area were calculated, and the medians of each parameter were 2.3 km, and 3, respectively. For each study, approximated survey area (ASA, $km^2$) was obtained by using the number of sampling stations in a unit area (NSSU) and total number of sampling stations (TNSS). To predict either appropriate ASA or NSSU/TNSS, we found and suggested statistically significant functional relationship among ASA, survey purpose and NSSU. This empirical approach will contribute to increasing sampling effort in a field survey and communicating with reasonable data and information in EIA and CCAU.

Optimization of the Addition of Garlic in Cheonggukjang using Response Surface Methodology (반응표면분석을 이용한 청국장 제조시 마늘의 첨가조건 최적화)

  • Hwang, Cho-Rong;Sim, Hye-Jin;Kim, Gyeong-Min;Cho, Kye-Man;Kim, Jeong-Hwan;Shin, Jung-Hye
    • Korean journal of food and cookery science
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    • v.29 no.6
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    • pp.661-669
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    • 2013
  • This study was performed to determine the optimal composition of Cheonggukjang added with garlic. The experiment utilized a central composite design (CCD). The evaluation was carried out by means of response surface methodology (RSM), which included 18 experimental points with three independent variables : the content of the garlic (1.3~9.7%, $X_1$), the steaming time of garlic (0~15.1 min, $X_2$), and the fermentation time of Cheonggukjang (48.2~71.8 h, $X_3$). The viscous substance ($Y_1$), acidity ($Y_2$), amino-type nitrogen ($Y_3$), ${\gamma}$-GTP activity ($Y_4$) and ABTS radical scavenging activity ($Y_5$). were assessed in four replicates with five dependent variables. The maximum content of the viscous substance was 13.02% at 6.53% ($X_1$), 6.81 min ($X_2$) and 55.18 h ($X_3$). The acidity was increased when the fermentation time was longer, and the minimum acidity point was 0.50% at 7.75% ($X_1$), 3.42 min ($X_2$) and 58.60 h ($X_3$), respectively. The content of the amino-type nitrogen at the experimental range studied was was 80.58~158.82 mg%, and the stationary point was at saddle point. Using ridge analysis, the maximum point was 156.97 mg% at 6.21% ($X_1$), 14.85 min ($X_2$) and 58.04 h($X_3$). The optimum conditions of ${\gamma}$-GTP activity was 5.73% ($X_1$), 6.99 min ($X_2$) and 57.96 h($X_3$), respectively, at the maximum point was 353.66 mU/mL. The maximum point of ABTS radical scavenging activity was 76.43% at 3.78% ($X_1$), 14.28 min ($X_2$) and 57.99 h($X_3$) at the saddle point, when the garlic steaming time was longer.