• Genes and Genomics
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• v.40 no.11
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• pp.1127-1136
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• 2018

The Greater Sudbury Region has been known as one of the most ecologically disturbed areas in Canada for the past century. Plant adaptation to environmental stressors often results in modifications in gene expression at the transcriptional level. The main objective of the present study was to compare the expression of genes associated with nickel resistance in Acer rubrum and Populus tremuloides growing in areas contaminated and uncontaminated with metals. Primers targeting Nramps4, Nas 3, At2G, MRP4 and alpha-tubulin genes were used to amplify cDNA of both species. The expression of the At2G gene, was $2{\times}$ and $9{\times}$ higher in P. tremuloides than in A. rubrum for St. Charles (uncontaminated site) and Kelly Lake (metal contaminated site), respectively. There was a much smaller difference between the two species for the Nramps 4 gene as its expression was $2.5{\times}$ and $3{\times}$ higher in P. tremuloides compared to A. rubrum from St. Charles and Kelly Lake, respectively. The same trend was observed for the MRP4 gene whose expression was $2{\times}$ and $14{\times}$ higher in P. tremuloides than in A. rubrum from St. Charles and Kelly Lake, respectively. For the Nas 3 gene, the expression was similar in both sites. This gene was upregulated $11{\times}$ and $10{\times}$ in P. tremuloides compared to A. rubrum in samples from St. Charles and Kelly Lake, respectively. In general, no significant difference was observed between the metal contaminated and uncontaminated sites for gene expression. In depth analysis revealed that AT2G and MRP4 genes were significantly down regulated in A. rubrum from the metal contaminated sites compared to those from uncontaminated areas, but environmental factors driving this differential gene expression couldn't be established.

• Fashion & Textile Research Journal
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• v.15 no.6
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• pp.899-911
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• 2013

This study investigated the current curricula for the training of textile design specialists. In-depth interviews were conducted with 6 practitioners working in the textile design industry; subsequently, the curricula offered by 20 textile design-related departments at 4-year and 2-year colleges in Korea were used for the data analysis. The results of this study were: First, the problems of textile design education were (1) a limited understanding of commercial designs, (2) an education system concentrated on pattern designs, (3) limited creativity and design expression capacity, (4) limited practical ability and the analysis of collection. Second, most textile design departments at Korean colleges were located in the Seoul/Gyeonggi district and Gyeongbuk/Gyeongnam district. Third, textile material and imagination/expression subjects were a limited part of the curricula; subsequently, a long-term and systematic education system (by college year) was required for the use of basic education subjects. Fourth, there was a shortage of practical subjects in college education despite the perception of commercial designs; in addition, a professional design education (by material and use) were an important part of practical business.

• Computers and Concrete
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• v.32 no.1
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• pp.1-13
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• 2023

This paper discusses a framework for predicting the flexural strength of prestressed and non-prestressed FRP reinforced T-shaped concrete beams using soft computing techniques. An analysis of 83 tests performed on T-beams of varying widths has been conducted for this purpose with different widths of compressive face, beam depth, compressive strength of concrete, area of prestressed and non-prestressed FRP bars, elasticity modulus of prestressed and non-prestressed FRP bars, and the ultimate tensile strength of prestressed and non-prestressed FRP bars. By analyzing the data using two soft computing techniques, named artificial neural networks (ANN) and gene expression programming (GEP), the fundamental parameters affecting the flexural performance of prestressed and non-prestressed FRP reinforced T-shaped beams were identified. The results showed that although the proposed ANN model outperformed the GEP model with higher values of R and lower error values, the closed-form equation of the GEP model can provide a simple way to predict the effect of input parameters on flexural strength as the output. The sensitivity analysis results revealed the most influential input parameters in ANN and GEP models are respectively the beam depth and elasticity modulus of FRP bars.

• International Journal of Advanced Culture Technology
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• v.12 no.2
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• pp.110-118
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• 2024

This study was conducted to analyze the metaverse usage behavior of college students through in-depth interviews and to explore the characteristics of the major metaverse platforms with a focus on keywords. This study derived elements such as metaverse participation activities, real-world order, metaverse system, presence, and self-expression from previous research. This study examined how college students who use the metaverse perceive these variables. We present the following research results: First, the college students who participated in the study showed interest in content with many interactive elements on the metaverse platform and appeared to participate more actively in such content. Second, college students cited the ability to engage in economic activities in the metaverse as their greatest strength. It was expected that social norms applied in the real world would also be applied in the metaverse. Third, the Metaverse system, such as the Metaverse UI, UX, and servers, was found to be a factor that needs to meet users' expectations and hinders immersion. In this study, the characteristics of the metaverse platform were analyzed, focusing on essential keywords from in-depth interviews. The analysis results are as follows: It was expressed with keywords such as Zepeto has 'avatar' and 'personality expression,' Ifland has 'free decoration' and 'communication convenience,' Gathertown has 'movement convenience' and 'video chat.' Roblox has 'game-based' and 'dynamic.'

• Development and Reproduction
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• v.10 no.4
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• pp.271-275
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• 2006

The objective of this study was to examine the early growth of haddock, Melanogrammus aeglefinus larvae from a series of reared specimens for provide information to developmental biology and more information on the aspect of aquaculture in the larvae of this species. Larvae were reared in the laboratory and sampled periodically for developmental study until 67 days after hatching. An increase in total length of fish indicated continuous growth, described by the growth expression of the type $TL=3.5374e^{0.0536X}(r^2=0.8759$, where TL is total length and X is at days after hatching) and $BW=0.0002e^{0.1858X}(r^2=0.8671$, where BW is body weight and X is at days after hatching), respectively. Pattern of body depth and pectoral fin length are instantaneous growth which expression of the type $BD=0.3545e^{0.0778X},\;r^2=0.9563$(where BD is body depth and X is at days after hatching) for body depth growth and the type $PL=0.0111e^{0.1591X},\;r^2=0.9194$(where PL is pectoral fin length and X is at days after hatching) for pectoral fin length growth. The relationship of body depth and total length expressed as $BD=0.2397X-0.5735(r^2=0.9957$, where BD is body depth and X is total length), and pectoral fin length and total length is $PL=0.1929X-1.3767(r^2=0.9882$, where PL is pectoral fin length and X is total length) pectoral fin length against body depth simultaneously recorded for juvenile haddock(PL=0.8117BD-0.9718, $r^2=0.9814$, where PL is pectoral fin length and BD is body depth). Relationship of body depth and body weight was expressed the type of $BD=-9.4734X^2+19.046X+1.3672,\;r^2=0.941$(where BD is body depth and X is body weight), and pectoral fin length and body weight expressed the type of $PL=6.379X^2+14.023X+0.3774,\;r^2=0.9494$(where PL is pectoral fin length and X is body weight). From this point view, growth characteristics of juvenile haddock in this experiment may be useful to establish a successful culture technique for rearing larval haddock.

• The Journal of the Korea Contents Association
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• v.10 no.7
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• pp.198-207
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• 2010

Formation of optical meaning shown in the picture book screen is the original expression method in that it makes you understand properties or characteristics of subjects more easily and faster adding another images to them, exaggerating or reducing them as well as shows the phenomenon that you try to deliver. In this study, I first prepared the framework of classification on metaphorical expression methods of the picture books' illustration through theoretical study on the optical metaphorical characteristics and expression forms to analyze how the metaphorical expression methods shown in the picture books for children works optically and psychologically to them. Also, I divided the expression form into 2 types to derive optical psychological perceptional response of children through the empirical survey and analyzed linguistic psychological response of children by type utilizing it as the survey materials of in-depth interviews. Finally, I think that the result of this study will help screen production based on personality and creativity of illustrators and, in addition, it will be utilized in studies and tests of the effective expression methods for students who learn illustration.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.14
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• pp.5601-5605
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• 2014

Background: Agrocybe aegerita Lectin (AAL) has been identified to have high affinity for sulfated and ${\alpha}2$-3-linked sialic acid glycoconjugates, especially the sulfated and sialyl TF (Thomsen-Friedenreich) disaccharide. This study was conducted to investigate the clinicopathological and prognostic value of AAL in identifying aberrant glycosylation in colorectal cancer (CRC). Materials and Methods: Glycoconjugate expression in 59 CRC tissues were detected using AAL-histochemistry. Clinicopathological associates of expression were analyzed with chisquare test or Fisher's exact test. Relationships between expression and the various clinicopathological parameters was estimated using Kaplan-Meier analysis and Cox regression models. Results: AAL specific glycoconjugate expression was significantly higher in tumor than corresponding normal tissues (66.1% and 46.1%, respectively, p=0.037), correlating with depth of invasion (p=0.015) and TNM stage (p=0.024). Patients with lower expression levels had a significantly higher survival rate than those with higher expression (p=0.046 by log rank test and p=0.047 by Breslow test for overall survival; p=0.054 by log rank test and P=0.038 by Breslow test for progress free survival). A marginally significant association was found between AAL specific glycoconjugate expression and overall survival by univariate Cox regression analysis (p=0.059). Conclusions: Lower AAL specific glycoconjugate expression is a significant favorable prognostic factor for overall and progress free survival in CRC. This is the first report about the employment of AAL for histochemical analysis of cancer tissues. The binding characteristics of AAL means it has potential to become a powerful tool for the glycan investigation and clinical application.

• Journal of Gastric Cancer
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• v.19 no.3
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• pp.315-328
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• 2019

Purpose: To investigate the clinical value of serum miR-221-3p and miR-122-5p expression levels in the diagnosis and prognosis of gastric cancer. Materials and Methods: Serum samples from 141 gastric cancer cases (gastric cancer group), 110 gastric polyps (gastric polyp group), and 75 healthy people (healthy control) were used to detect miR-221-3p and miR-122-5p expression using real-time reverse transcription polymerase chain reaction. Results: Serum miR-221-3p expression was significantly higher in the gastric cancer group than in the gastric polyp group, and it was significantly lower than that before operation. The miR-221-3p expression was significantly higher in the death group than in the survival group. The proliferation and migration ability significantly increased and the apoptosis rate significantly decreased by miR-221-3p transfection in gastric cancer cells. In contrast, the function of miR-122-5p in gastric cancer cells was opposite of miR-221-3p. Serum miR-221-3p expression was negatively correlated with that of miR-122-5p in gastric cancer. Serum miR-221-3p and miR-122-5p expressions were significantly correlated with the degree of differentiation, tumor, node, metastasis stage, lymph node metastasis, and invasion depth. miR-221-3p and miR-122-5p expression levels were independent prognostic factors for postoperative gastric cancer. In the diagnosis and predicting prognosis of gastric cancer, receiver operating characteristic analysis revealed that the area under curve of combined detection of serum miR-221-3p and miR-122-5p expression had a greater diagnostic effect than either single maker. Conclusions: The miR-221-3p and miR-122-5p are involved in the development of gastric cancer, and they have important clinical values in gastric cancer diagnosis and prognosis.

• The Journal of the Korea Contents Association
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• v.9 no.6
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• pp.104-116
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• 2009

Today's Digital Environment made Typography to enter into a new phase. The Type which is a principal component of Typography is no exception to this aspect. However since the existing two-dimensional space has figurative potential, Visual Communication designers are still striving to explore both X axis and Y axis. In this context, Z axis which signifies 'Depth' is often recognized as an unfamiliar mathematical concept unrelated to Design Sensibility. If Z axis is used, the figurative aspect can be acquired, which was hard to be realized in the flat space, because the additional space of depth allows the expansion of omni-directional expression of target. Most notable is the fact that Z axis provided by Digital Environment has actually long existed and is inseparable from Type. This study, therefore, focuses on the three-dimensional expression of Type in the Visual Communication Design area by taking into account the above characteristics.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.134-141
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• 2016

To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor ${\alpha}$ (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor.