• YAKHAK HOEJI
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• v.37 no.5
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• pp.504-510
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• 1993

Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3'-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5'-regulatory region(G2101$\rightarrow$AGG); IVS I(T2401$\rightarrow$A); Exon II(C2411 deletion); IVS II(A2740 dejection). The variations at the G2101 and T2401 were the same as those found in one American allele. The other two variations were observed only in the specific Korean allele. And, the enzyme digestion patterns among normal, insulin dependent diabetes mellitus, and non-insulin dependent diabetes mellitus were the same. On the other hand, PCR method showed the possibility of the quickaccess for the polymorphic region in terms of the restriction fragment length of polymorphism.

• Journal of Plant Biology
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• v.38 no.2
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• pp.129-136
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• 1995

Complementary DNA of the genomic RNA of odontoglossum ringspot virus Cymbidium strain (ORSV-Cy) was synthesized from polyadenylated viral RNA and cloned. Selected clones containing the viral RNA-dependent RNA polymerase gene of the virus has been sequenced by automated sequencing system. The complete nucleotide sequence of an open reading frame is 1377 base pairs in length, and encodes a protein of 458 amino acids about 52, 334 D. The 52 kD protein of ORSV shares four sequence motifs characteristic of viral RNA-dependent RNA polymerase. Comparison of the ORSV 52 kD protein sequence with that of other five viruses in tobamovirus group showed 76.0 to 60.7% homologies at the amino acid level and the conservation of the four motifs betwen the viruses.

• Journal of the Korean Institute of Telematics and Electronics
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• v.13 no.4
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• pp.1-5
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• 1976

Spectrum의 시간기복을 나타내는 시간 및 주파수의 함수 F(w,t,T) 및 W(w,t,T)를 정의하였고, 실례에 적용시켰다. Fourier변환을 f(t)와 Phasor ejwt와의 Correlation으로 정의하였고 그 변환시분이 시간마다의 성분의 합계이어서 t 시각에서의 Spectrum을 그 근거에서의 f(t)값에만 의함을 증명하였다. Two new time functions defining Time Dependent Fourier Transform F(w,t,T) and Time Dependent Spectrum Density W(w,t,T) are deduced. courier transform is defined as the correlation between the time function f(t) and phasor ejwt. Several theorems corncerning the new functions are proved in order to verify the instantaneous cause a effet of the function f(t) and the nuctuating spectrum.

• Journal of Korean Institute of Industrial Engineers
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• v.32 no.2
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• pp.98-103
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• 2006

In this research, the single machine capacitated lot-sizing and scheduling problem with sequence- dependent setup costs and setup times (CLSPSD) is considered. This problem is the extension of capacitated lot-sizing and scheduling problem (CLSP) with an additional assumption on sequence-dependent setup costs and setup times. The objective of the problem is minimizing the sum of production costs, inventory holding costs and setup costs satisfying customers' demands. It is known that the CLSPSD is NP-hard. In this paper, the MIP formulation is presented. To handle the problem more efficiently, a conceptual model is suggested, and one of the well-known meta-heuristics, the simulated annealing approach is applied. To illustrate the performance of this approach, various instances are tested and the results of this algorithm are compared with those of the CLPEX. Computational results show that this approach generates optimal or nearly optimal solutions.

• Journal of Korean Institute of Industrial Engineers
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• v.40 no.3
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• pp.305-312
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• 2014

This research deals with a problem that minimizes makespan in a non-identical parallel machine system with sequence and machine dependent setup times and machine dependent processing times. We first present a new mixed integer programming formulation for the problem, and using this formulation, one can easily find optimal solutions for small problems. However, since the problem is NP-hard and the size of a real problem is large, we propose four heuristic algorithms including genetic algorithm based heuristics to solve the practical big-size problems in a reasonable computational time. To assess the performance of the algorithms, we conduct a computational experiment, from which we found the heuristic algorithms show different performances as the problem characteristics are changed and the simple heuristics show better performances than genetic algorithm based heuristics for the case when the numbers of jobs and/or machines are large.

• Geomechanics and Engineering
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• v.12 no.3
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• pp.347-360
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• 2017

The problem of cylindrical cavity expansion incorporating deformation dependent of intermediate principal stress in rock or soil mass is investigated in the paper. Assumptions that the initial axial total strain is a non-zero constant and the axial plastic strain is not zero are defined to obtain the numerical solution of strain which incorporates deformation-dependent intermediate principal stress. The numerical solution of plastic strains are achieved by the 3-D plastic potential functions based on the M-C and generalized H-B failure criteria, respectively. The intermediate principal stress is derived with the Hook's law and plastic strains. Solution of limited expansion pressure, stress and strain during cylindrical cavity expanding are given and the corresponding calculation approaches are also presented, which the axial stress and strain are incorporated. Validation of the proposed approach is conducted by the published results.

• Journal of the Korean Mathematical Society
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• v.43 no.6
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• pp.1325-1338
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• 2006

For double arrays of constants ${a_{ni},\;1{\leq}i{\leq}k_n,\;n{\geq}1}$ and sequences of negatively orthant dependent random variables ${X_n,\;n{\geq}1}$, the conditions for strong law of large number of ${\sum}^{k_n}_{i=1}a_{ni}X_i$ are given. Both cases $k_n{\uparrow}{\infty}\;and\;k_n={\infty}$ are treated.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.51 no.6
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• pp.296-301
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• 2002

Modern power systems are very complex and to model them completely is impractical for electromagnetic transient studies. Therefore areas outside the immediate area of interest must be represented by some form of Frequency Dependent Network Equivalent (FDNE). In this paper a method for developing Frequency Dependent AC system Equivalent (FDACSE) using Z-domain rational Function Fitting is presented and demonstrated. The FDACSE is generated by Linearized Least Squares Fitting(LSF) of the frequency response of a Z-domain formulation. This 1 & 2 port FDACSE have been applied to the New Zealand South Island AC power system. The electromagnetic transient package PSCAD/EMTDC is used to assess the transient response of the 1 & 2 port FDACSE developed under different condition (linear load, fault and nonlinear loading). The study results have indicated the robustness and accuracy of 1 & 2 port FDACSE for electromagnetic transient studies.

• Atmosphere
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• v.22 no.3
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• pp.309-319
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• 2012

It is important to characterize and quantify the inherent error in the radar rainfall to make full use of the radar rainfall. This study verified the error structure of the reflectivity and corrected the range dependent error in the CAPPI using a VPR (vertical profile of reflectivity) model. The error of the CAPPI to display the reflectivity data becomes bigger for the range longer than 100 km. This range dependent error, however, is significantly improved by corrected the CAPPI data using the VPR model.

• International Journal of Control, Automation, and Systems
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• v.6 no.4
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• pp.515-525
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• 2008

In this paper, the problem of delay-dependent stabilization for singular systems with multiple internal and external incommensurate constant point delays is investigated. The condition when a singular system subject to point delays is regular independent of time delays is given and it can be easily test with numerical or algebraic methods. Based on Lyapunov-Krasovskii functional approach and the descriptor integral-inequality lemma, a sufficient condition for delay-dependent stability is obtained. The main idea is to design multiple memoryless state feedback control laws such that the resulting closed-loop system is regular independent of time delays, impulse free, and asymptotically stable via solving a strict linear matrix inequality (LMI) problem. An explicit expression for the desired memoryless state feedback control laws is also given. Finally, a numerical example illustrates the effectiveness and the availability for the proposed method.