• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.4
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• pp.749-760
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• 1998

The main purpose of this study was to evaluate the effect of dentin with various pretreatment application time on the dentin surface structure and the bond strength of glass ionomer restorative matrials between primary and permanent tooth. There are difference of composition, structure and pretreated effect of dentin conditioning between the primary and permanent teeth. Inspite of thease fact, we used the same application time of permanent tooth. This study intends to determine the most effective application time using SEM analysis of the surface change by dentin conditioning. Forty five intact primary and permanent teeth were splited mesiodistally with seperating diamond disk. Each eight specimens were prepared for shear bond strength test. (randomely devided eight group as follows:) group I conditioning for five seconds group II conditioning for ten seconds group III conditioning for fifteen seconds group IV conditioning for twenty seconds Additional specimens were prepared to SEM analysis. The result are as follows: 1. Primary teeth are higher shear bond strength in group I and group II. 2. There was no statistically significant difference between primary and permanent teeth in group III and group IV. 3. In SEM analysis, smear layer removal by dentin pretreatment of primary teeth was faster than permanent teeth. Primary teeth may require shorter pretreatment time of dentin for G-I restoration than that of permanent teeth.

• Restorative Dentistry and Endodontics
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• v.17 no.1
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• pp.36-54
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• 1992

The purpose of this study was to compare the shear bond strengths to ground dentin surfaces of four dentinal bonding agents in 193 teeth. Various dentin surfaces treated with four dentin bonding agents were attached with two restorative composite resins. The effectiveness of the bonding were tested by the monitoring the shear bond strength. The shear bond strengths were measured after 2 hours and 24 hours after surface conditioning with four dentin bonding agents. Effects of EDTA, the additive illumination, and sealer treatments without primer on bond strength to dentin surfaces were assessed. In addition the effects of the thickness of specimens ranging from 0.65 mm to 1.95 mm and the ratio of catalyst and base paste on the bond strength of chemical cure composite resin were estimated. The shear bond strength was determined by testing specimens in the Instron universal testing machine (Model No. 1122) at a crosshead speed of 1.0 mm/min. Following condusions were drawn: 1. The highest mean shear bond strengths of chemical cure composite resin to dentin conditioning with dentin bonding agents aged 2 hours were obtained, and then that was decreased with time followed by EDTA treatment. 2. In light cure composite resin, the shear bond strength was increased following dentin conditioning with bonding agents with time, irradiation time and EDTA treatment except in SB group. 3. The thicker the composite resin specimen was, the less the shear bond strength in chemical cure composite resin was. 4. In light cure composite resin, there was a little change in shear bond strength following dentin conditioning with bonding agents. 5. In chemical cure composite resin, the shear bond strength was the highest in the ratio of 1/1 of catalyst and base part. 6. Without a dentin primer, shear bond strength to dentin conditioned only with UB sealer was the highest among four sealers in light cure composite resin.

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.227-241
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• 1996

The effect of collagen dissolution in acid conditioned dentin was morphologically examined by both scanning and transmission electron microscopy. 18 freshly extracted human molars and dentin bonding systems of All Bond 2, Scotchbond Multipurpose, Superbond D-Liner were used in this study. For SEM preparation, each 3 of ~ exposed dentin surfaces were acid conditioned by using various acids within the above three bonding systems respectively. After acid conditioning of the other 3 exposed dentin surfaces as above, they were treated with 1.7% NaOCl for 2 minutes. The remaining 3 dentin surfaces were acid conditioned and treated with 3.3 % NaOCl for 2 minutes. All of the specimens were then fixed in 4 % glutaraldehyde for 12 h at $4^{\circ}C$ and dehydrated in ethanols grades from 50 % to 100 %, then surface changes of the specimens were observed by using SEM. For TEM preparation, exposed dentin surfaces were acid conditioned with the same acid as SEM specimens and treated with 1.7%, 3.3 % NaOCl respectively, then applied with corresponding bonding agents. After the procedures were finished, composite resin were applied on the dentin surfaces and light cured. Small, rectangular sticks with end dimensions of approximately 1 by 1 mm were sectioned and further sample preparative techniques for transmission electron microscopy were performed in accordance with the procedures used for ultrastructural TEM observations of calcified tissues. The results were as follows : 1. In the 1.7 % NaOCl retreated specimens after acid conditioning, the porous dentin surface of intertubular dentin and wide opening of dentinal tubules were appeared. And there were fine irregularities on the intertubular dentin, indicating a clear difference as compared with the acid conditioned specimens. 2. In the 3.3% NaOCl retreated specimens after acid conditioning, the intertubular dentin was further eroded causing a more porous and wider opening of dentinal tubules. Moreover, sharp irregularities on the intertubular dentin were more evident than those of acid conditioned and 1.7% NaOCl retreated specimens. 3. In all of the acid conditioned specimens, the resin-dentin hybrid layer of approximately 3.5mm thickness was formed and the collapsed collagen layer was observed on the uppermost part of hybrid layer in the specimens applied with All Bond 2. The collgen fibrils of intertubular dentin in specimens applied with Scotchbond Multipurpose were running perpendicular to the interface, and electron dense black layer demarcated from the deep unaltered dentin was more evident in the specimen applied with Superbond D-Liner than any other specimens. 4. In the 1.7 % NaOCl retreated specimens after acid conditioning, the resin-dentin hybrid layer of approximately 2.5-3.0mm thickness was formed and the collapsed collagen layer and longitudinally running collagen fibrils as shown in the acid conditioned specimens were observed in the specimens applied with All Bond 2 and Superbond D-Liner. 5. In all of the 3.3% NaOCl retreated specimens after acid conditioning, the evidence of resin-dentin hybrid layer was not identified ; nevertheless, the longitudinally running collagen fibrils remained slightly in the specimens applied with All Bond 2.

• Restorative Dentistry and Endodontics
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• v.18 no.1
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• pp.173-186
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• 1993

The purpose of this study is to observe the morphological changes of prepared dentin surfaces by 5 dentin conditioning agents. Freshly extracted 48 healthy human molars were used in this study. The teeth were stored at $4^{\circ}C$ physiologic saline solution befor experiment. The teeth were cross-sectioned to expose dentin below 3.0mm at the cusp tip and above 2.0mm at the cemento-enamel junction with Crystal Cutter (MC411 D, Maruto Co., Japan). The specimens were then divided into 12 groups. The sectioned dentin surfaces in group 1, 3, 5, 7, 9, and 11 were prepared with No. 301 diamond point under air-water spray and those in group 2, 4, 6, 8, 10, and 12 were prepared with No. 700 carbide bur. The prepared dentin surfaces were conditioned with Nitric acid, Citric acid, Poly acrylic acid, EDTA, and Phosphoric acid. All the specimens were gold-coated with Eiko ion coater (Eiko-engineering Co.) and observed in Hitachi S-2300 Scanning electron microscope at 20 KV. The following results from this study were obtained; 1. The dentinal smear layers prepared with diamond point were compacted than those prepared with the carbide bur. 2. The dentinal smear layers prepared with diamond point or carbide bur were thick but after treatment of dentin conditioning agents smear layers were removed almost. 3. Irrespective of the uses of the diamond point or the carbide bur the morphological changes of dentin surfaces treated with the same conditioning agents were similar. 4. Treatment of nitric acid and EDTA was a little effect in removing dentinal smear layer. 5. Treatment of citric acid and phosphoric acid removed the smear layer very effectively and showed dissolution of peritubular dentin and opening of dentinal tubules.

• Restorative Dentistry and Endodontics
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• v.17 no.1
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• pp.104-114
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• 1992

The purpose of this study was to evaluate the shear bond strength of glass ionomer cement(Ketacfil, ESPE, Co.) against dentin surface which had been treated with surface conditioning agents(distilled water, 5% sodium hypochlorite solution, Ketac - conditioner, 40% polyacrylic acid). In this study, 60 human molars with sound and healthy crown portion which were previously extracted for orthodontic or periodontal problem. The dentin surfaces of these teeth were exposed with wet trimmer and polished with 150 - grit and 600 - grit silicon carbide paper and the teeth were divided into four groups(15 teeth per group) according to the following surface conditioning methods. Group I : Surface treatment with distilled water as control group. Group II : Surface conditioning with 5% sodium hypochlorite solution. Group III : Surface conditioning with Ketac conditioner. Group IV : Surface conditioning with 40% polyacrylic acid. The shear bond strengths were measured by Autograph(Shimatzu Co. Japan). The result of the evaluations were then subjected to statistical analysis using one - way analysis of variance and Duncan test and the results were as follows : 1. The shear bond strength accrding to the dentin surface conditioning conditions was highest in Ketac conditioner group, with measurements of $44.44{\pm}0.74(kg/cm^2)$ and lowest in the distilled water group, with measurements of $28.84{\pm}0.88(kg/cm^2)$. 2. Statistically significant differences were found between surface conditioning with 5% sodium hypochlorite solution group or Ketac conditioner group and distilled water group(P<0.01). 3. Also, statistically significant difference was found between surface conditioning with distilled water group and 40% polyacrylic acid group(P<0.05). 4. Overall difference in statistical significance between the groups was not found (P<0.05). 5. Fractured dentin surface treated with conditioning solutions showed cohesive fracture. 6. Distilled water group and 5% sodium hypochlorite solution group removed the smear layer less effectively. 7. Conditioning dentin with Ketac conditioner and 40% polyacrylic acid resulted in the removal of a significant amount of the smear layer without removing the tubular plugs and dissolving the peritubular dentin.

• Restorative Dentistry and Endodontics
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• v.14 no.2
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• pp.65-76
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• 1989

The purpose of this study was to observe the effect on the removal of dentinal smear layer and morphological changes of reduced dentin surfaces by various dentin surface conditioners. Thirty-two healthy human premolars extracted due to periodontal or orthodontic reasons were used. The teeth were cross-sectioned to expose dentin at the middle portion of the crown with diamond rotary saw. The specimens were then divided into 8 groups. The sectioned dentin surfaces in group 1 to 4 were grinded with No. 400 grit silicone abrasive paper and those in group 5 to 8 were cut with #700 carbide bur under air-water spray. The grinded or cut dentin surfaces were conditioned with 3% $H_2O$, Dentin Conditioner(GC Inter. Corp., Japan), and Scotchprep(3M Dent Prod., U.S.A) according to manufacturer's directions. All the specimens were dried in room temperature for 48 hours, and gold-coated with Eiko ion coater(Eik-engineering Co.), and observed in Hitachi S-450 Scanning electron microscope at 15-25 KV. The following results were obtained; 1. The dentin surfaces grinded with the silicon abrasive paper were rougher in texture and heavier in amount of smear layer than those cut with the carbide bur. 2. Scrubbing of 3% $H_2O_2$ was not effective in removing dentinal smear layer. 3. 20-second conditioning of Dentin Conditioner (GC Inter. Corp) resulted in the removal of a significant amount of the smear layer without removing the tubular plugs and dissolving the peritubular dentin. 4. Scotchprep removed the smear layer very effectively. But at the same time it dissolved the peritubular dentin. 5. Irrespective of the uses of the silicon carbide abrasive paper or the carbide bur the morphological changes of dentin surfaces treated with the same conditioning agents were similar.

• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.468-476
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• 1998

The purpose of this study was to observe the morphologic change of dentinal surface, adhesion in interface between dentin and bonding agents, and penetration pattern of resin tags into dentinal tubles according to bonding procedure of ONE-STEP universal adhesive system. Ten extracted human molars were mounted in dental stone and sectioned to expose mid-coronal occlusal dentin and again sectioned tooth crown apically. Specimens were randomly assigned to three groups for dentin conditioning with 32% phoshoric acid, two coats of bonding agents after dentin conditioning, and bond of composite resin. The surfaces of dentin were treated with etch ant and applied bonding agent, and bonded composite resin according to the directions of manufacturer. Specimens which were boned composite were sectioned longitudinally for observing interfaces between resin and dentin. Two of specimens which were sectioned longitudinally were immersed in 6 N HCL for 30 seconds and 1% NaOCL for 12 hours to partially demineralize and deproteinize the dentin substrate. Each specimen was mounted on a brass stub, sputter-coated with gold and observed under SEM. The result were as follows : 1. On the dentinal surface which was conditioned with 32% phosphoric acid. the smear layer was completely removed. orifices of dentinal tubules were opened 3-$5{\mu}m$ wide. and dentinal surface was irregular. 2. On the dentinal surface which was applied ONE-STEP. bonding agent. resin particles were observed on the orifices of dentinal tubules and intertubular dentin. 3. There were close adaptation between dentin and resin and were the pattern which composite invaded into dentin. 4. 1-$3{\mu}m$-wide hybrid layer was visible in the interface between dentin and resin. 5. Long and funnel shaped resin tags were observed in demineralized specimens. and the surfaces of tags were rough.

• Journal of Periodontal and Implant Science
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• v.40 no.6
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• pp.257-264
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• 2010

Purpose: Connective tissue reattachment to periodontally damaged root surfaces is one of the most important goals of periodontal therapy. The aim of this study was to develop a root conditioning agent that can demineralize and detoxify the infected root surface. Methods: Dentin slices obtained from human teeth were treated with a novel root planing agent for 2 minutes and then washed with phosphate-buffered saline. Smear layer removal and type I collagen exposure were observed by scanning electron microscopy (SEM) and type I collagen immunostaining, respectively. Cell attachment and lipopolysaccharides (LPS) removal demonstrated the efficiency of the root conditioning agent. Results: SEM revealed that the smear layer was entirely removed and the dentinal tubules were opened by the experimental gel. Type I collagen was exposed on the surfaces of the dentin slices treated by the experimental gel, which were compared with dentin treated with other root planing agents. Dentin slices treated with the experimental gel showed the highest number of attached fibroblasts and flattened cell morphology. The agar diffusion assay demonstrated that the experimental gel also has effective antimicrobial activity. Escherichia coli LPS were effectively removed from well plates by the experimental gel. Conclusions: These results demonstrated that this experimental gel is a useful tool for root conditioning of infected root surfaces and can also be applied for detoxification of ailing implant surface threads.

• Journal of Periodontal and Implant Science
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• v.32 no.1
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• pp.13-23
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• 2002

The ultimate goal of periodontal therapy is directed to arresting the progression of the disease, and regenerating the fibrous attachment. In order to achieve such treatment aim, the plaque and calculus must be eliminated and the physiological conditions of the root surface must be changed to facilitate the attachment and migration of the new fibroblasts, The method of changing the proper root surface conditions to promote the healing of periodontal tissue involves mechanical procedures, such as scaling and root planing, and chemical procedures such as tetracycline-HCl. However, the formation of a long junctional epithelium was most frequently observed type of healing. Thus, the aim of this study was to examine in vitro the influence of surface conditioning of dentin by TC-HCl on human gingival epithelial cell attachment. Human gingival epithelial cells were obtained from healthy retromolar pad area(under the age 23 years). Seventy two teeth extracted from severe periodontitis were used as study material. To evaluate the epithelial cell attachment to dentin, the prepared specimen was divided to four groups. For the control group, only scaling and root planing were carried out, and for the test group, 1 to 3, the concentration of the TC-HCl was 50, 125 and 250mg/ml respectively. After cell cultivation time of 1-, 3-. 24 hour, for the indirect quantitative assessment of gingival epithelial cell attached to dentin sample, the absorbance of epithelial cell unattached to dentin was measured. The results were as follows; 1. There was no statistically significant difference between scaling and root planing group and TC-HCl 50mg/ml 125mg/ml and 250mg/ml group about absorbance of unattached epithelial cell to dentin sample(p>0.5). 2. As time passes, the absorbance of unattached gingival epithelial cell to dentin sample was decreased statistically significant(p<0.05). 3. There was no statistically significant difference among the TC-HCl group(p>0.05) We concluded that there was similar effect on gingival epithelial cell attachment between TC-HCl conditioning on root surface and only scaling and root planing treatment

• Proceedings of the KACD Conference
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• 2003.11a
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• pp.625-625
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• 2003

I. Objectives Self-etching primer adhesive system is affected to dentin surface conditioning and priming. Especially application time of self-etching primer is very important factor of clinical procedure which has direct influence on smear layer, etching reaction and primer penetration to dentin. This study evaluated the influence of application time of self-etching primers on microtensile bond strength (${\mu}{\;}TBS$) to dentin using three self-etching primer adhesive systems.(omitted)