• Title/Summary/Keyword: Densitometry

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Changes in Electrophoretic Patterns of Egg White Proteins during Incubation (부화과정에 있어 난단백의 변화에 대하여)

  • Lee, C.Y.;Jo, D.H.;Cheon, H.W.
    • Applied Biological Chemistry
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    • v.14 no.3
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    • pp.201-205
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    • 1971
  • The electrophoretic patterns of the thin and thick layers of EWP were observered and the densitometry was conducted. 1. Most characteristic were the patterns of both layers at 7-day incubation compared with those of fresh stage. 2. After 15 days the patterns of both layers were almost same. 3. The ratio of some components of the fresh EWP is in a good accordance with the other wisely measured data.

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Studies on Quality Evaluation of Crude Drug Preparation(II) -Analysis of Saengmaek-san by Thin Layer Chromatography and High Performance Liquid Chromatography- (생약 복합제제의 품질평가에 관한 연구(제 2 보) -생진산(生賑散)의 품질평가 방법에 관하여-)

  • Hong, N.D.;Kim, J.W.;Won, D.W.;Kong, Y.C.;Kim, N.J.;Joo, S.M.
    • Journal of Pharmaceutical Investigation
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    • v.17 no.1
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    • pp.22-30
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    • 1987
  • Evaluation method of crude drug preparations was studied in Saengmaek-san. Zig-zag TLC scanning profiles and high performance liquid chromatograms were obtained from Saengmaek-san and its each crude drug. A method using TLC densitometry and high performance liquid chromatography was established for the precise determination of ginsenoside $Rb_1$ in Saengmaek-san containing Ginseng Radix. Consequently, ginsenosicle $Rb_1$ content was 0.45-0.48 mg per g of Saengmaek-san. This method was found to be useful for the quality evaluation of oriental medicinal preparations.

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Separation of Lactoferrin from Model Whey Protein Mixture by Reverse Micelles Formed by Cationic Surfactant

  • Noh, Kyung-Hyun;Rhee, Min-Suk;Imm, Jee-Young
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.131-136
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    • 2005
  • The selective extraction behavior of lactoferrin (Lf) from whey protein mixture was examined using reverse micelles formed by the cationic surfactant, cetyldimethylammonium bromide (CDAB). The major whey proteins, including ${\beta}$-lactoglobulin, ${\alpha}$-lactalbumin and bovine serum albumin, were solubilized from aqueous phase to organic phase while Lf was recovered in the aqueous phase. The solubilization behaviors of the proteins were manipulated by the process parameters such as the pH and salt concentration of the aqueous phase and the surfactant concentration in the organic phase. Efficient forward extraction was achieved with sodium borate buffer (50 mM, pH 9) containing 50 mM KCl and organic phase containing 100 mM CDAB. Based on SDS-PAGE and densitometry, about 96% of the initial Lf remained in the aqueous phase after forward extraction. The dialyzed Lf fully maintained its bacteriostatic activity against E. coli O157:H7.

Highly Economic and High Quality Zinc-flake Manufacturing by High Kinetic Processing

  • Ren, H.;Benz, H.U.;Chimal V., O.;Corral G., M.S.;Zhang, Y.;Jaramillo V., D.;Zoz, H.
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.975-976
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    • 2006
  • The present paper is a parameter study of zinc flake production using a Simoloyer CM01 horizontal high energy rotary ball mill. The manufactured flakes have a dimension in thickness (t) < $1{\mu}m$ and diameters (d) 5-100 ${\mu}m$, consequently a ratio d/t up to 200. The flake geometry is mainly controlled by the variation of process parameters such as rotary speed of the rotor, ratio of powder/ball charge, load ratio of the system, process temperature, operating model and the quantity of process control agent (PCA). The Zn flakes were characterized by SEM, tap densitometry, laser diffraction and water coverage measurement.

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Characterization of Soot Particles Generated in Non-sooting and Sooting Normal Diffusion Flames (Sooting 및 Non-Sooting 정상 확산 화염에서 생성되는 매연 입자의 특성에 대한 연구)

  • Choi, In-Chul;Lee, Jae-Bok;Hwang, Jung-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.7
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    • pp.984-993
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    • 2000
  • Characteristics of carbon soot particles generated in diffusion flames were studied. Non-sooting and sooting normal diffusion flames using propane or ethylene as fuel were selected. In the flames, soot volume fraction was measured by a thermocouple, and primary particle diameter and cluster size were analyzed by TEM photographs. The characteristics of soot particles depended on flame(non-sooting or sooting) and fuel(propane or ethylene) type. Unlike the sooting diffusion flames, particle growth and oxidation processes were clearly observed in the non-sooting diffusion flames. In the sooting diffusion flames, soot particle size was slightly changed at the flame tip.

Detection of N,N-Dimethylaniline in Ampicillin Trihydrate by Thin Layer Chromatography and Densitometry (박층크로마토그라피 및 농도 측정법에 의한 Ampicillin Trihydrate 중의 N,N-Dimethylaniline의 검색)

  • 이왕규;김박광;심창구
    • YAKHAK HOEJI
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    • v.22 no.4
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    • pp.238-241
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    • 1978
  • A simple and convenient method of detecting N, N-dimethy laniline (D.M.A.) residues in ampicillin trihydrate (A.T.) was established. D.M.A. was extracted by chloroform from the chloroform presaturated N/10-ammonia water solution of A.T. and chromatographed on silica gel G thin layer. Blue spot appeared in 15minutes after spray of 2, 6-dichloroquinonechlorimide solution was compared with the blue spot of reference concomitantly processed. The developing solvent was prepared by mixing equal volume of cyclohexane and chloroform. To quantitate the amount of D.M.A. in A.T., T.L.C. was performed with the Eastmann Chromatogram sheet, then color density was measured by Cosmo Superclick densitometer. The developing solvent at this time was cyclohexane-chlorofonn (3+7) mixture. The peak areas obtained with the amount of D.M.A ranging from 0.05 to 2.0 .mu.g were linear to color density. Better sensitive results would be available with the densitometer equipped with monochromator.

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BMD Analysis according to the Obesity Index in a Group of Adults (비만도에 따른 성인들의 집단별 골밀도 분석)

  • Kim, Sun-Geun;Kweon, Dae-Cheol;Oh, Chan-Ho;Song, Woon-Heung
    • Korean Journal of Digital Imaging in Medicine
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    • v.10 no.1
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    • pp.51-58
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    • 2008
  • Obesity which causing many physical and psychological problems is difficult to treatment. The purpose study was investigate relationships between weight, height, body mass index (BMI) and BMD of according to the low, normal, over, obesity, high group. According to obesity index, each group was divided into five proup. Study subjects were man(n=135) and woman(n=145). We checked the BMD of femoral neck and lumbar spine by using dual energy X-ray bone densitometry. Weight was positively correlation with height (p<0.05) by Pearson's correlation matrix. There was a significant difference in BMD of femoral neck and lumbar spine between four(low, normal, over, obesity) groups except for high obesity groul.

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Automatic Detection Method of the Region of Interest in the Measurement of Bone Mineral Density by Ultrasound Imaging (초음파 영상에 의한 골밀도 측정에서 관심영역의 자동 검출방법)

  • 신정식;안중환;한은옥;김형준;한승무
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.11
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    • pp.200-208
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    • 2004
  • In ultrasonic bone densitometry, the positioning of measurement site is decisive in precision and reproducibility. In this study, automatic Region of Interest (ROI) detection algorithm is suggested and adopted the method using the local minimum value by ultrasonic image. The preprocess before the local minimum method extracts out the bone area and calculates the geometrical information of bone. The developed ROI detection algorithm was applied to the clinical test for the subject of 305 female patients in the range of 22-88 years old. As the results, the accuracy of the algorithm was shown to be 98.3%. It was also found that bone density parameter was significantly correlated with age(r=0.85, p<0.0001).

Excess Volumes of Aqueous Solutions of Nonionic Amphiphile 2-(2-Hexyloxyethoxy)ethanol (비이온 양친매성 분자 2-(2-Hexyloxyethoxy)ethanol 수용액의 과잉 부피)

  • Lee, Jungno;Hwang, Yoonmi;Kang, Minhee;Lim, Kyung-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.3
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    • pp.516-525
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    • 2020
  • The densities of aqueous solutions of the amphiphile 2-(2-hexyloxyethoxy)ethanol (C6E2) were measured at 279.15 K and 282.15 K by vibrating-tube densitometry. Then using the density data of the binary C6E2 (1)/water(2) system, the excess volumes and partial molar volumes were determined at various compositions. Excess volume VE exhibits negative deviation for the whole region of composition, which implies relatively stronger attraction between molecules. At the C6E2 mole fraction of around 0.45, VE was at its minimum. Partial molar volume ${\bar{V}}_1$ increases monotonously with the mole fraction x1(=x) and ${\bar{V}}_2$ decreases with x. Any particular point in ${\bar{V}}_1$ and ${\bar{V}}_2$, which may point to molecular association, was unobserved.

Relationship of Bone Mineral Density Measured by Ultrasound Bone Densitometry and Body Composition or Backmuscle Strength (초음파방식으로 측정된 골밀도와 신체조성 및 배근력의 관계)

  • Lee, Won-Jeong
    • Journal of the Korean Society of Radiology
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    • v.13 no.5
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    • pp.721-728
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    • 2019
  • The purpose of this study was to evaluate the relationship between bone mineral density (BMD) and body composition (BC) or backmuscle strength (BS). Sixty-one participants were measured by BMD using ultrasound bone densitometry and Inbody for BC (i.e., SLM=Soft lean mass, SMM=Skeletal muscle mass, FS=Fitness score. et al.), BS after self-questionnaire for life cycle. This study was performed after approving Institutional Review Board and obtaining the informed concent from all participants. Participants was divided into two group by BMD T-score; $T-score{\geq}-1.0$, T-score<-1.0. Statistical analysis was performed by using SPSS ver. 22.0(USA), Spearma test for correlation between BMD and BC or BS. BMD or SLM, BS was increased with increasing physical activity or body mass index. SMM or SMM, FS of BMD $T-score{\geq}-1.0$ group was higher than that of T-score <-1.0 group as well as BS(p>0.05). BMD T-score was correlated positively with SLM(r= 0.424) or SMM(r= 0.431) in men, as well as in women(p<0.05). BS was correlated positively with SLM or SMM, FS. BS was significantly positive correlated with BMD in women group (r= 0.591, p= 0.001), but not in men group. We concluded that BMD is the relationship with SLM or SMM, as well as BS in women.