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Transmission electron microscopic ultastructure of the tegument of Fibricola seoulenis (Fibricola seouenis 표피의 투과전자현미경적 미세구조)

  • 손운목;이순형
    • Parasites, Hosts and Diseases
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    • v.31 no.4
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    • pp.301-314
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    • 1993
  • An electron microscopic study was performed to observe the ultrastructure of the tegument of U seoulensis. The outer surface of the tegument was covered with a tnlaminated plasma membrane. The electron-dense cytoplasmic layer was $2.5{\;}\mu\textrm{m}$ wide In the anterior portion and contained numerous vacuoles, mitochondriae and granular materials in its matrix. The basement layer was 330 nm wade or so, and Its numerous extensions protruded into the cytoplasmic layer. The sensory organ was composed of a small vesicle of $1.7{\;}{\times}{\;}1.1{\;}\mu\textrm{m}$ in dimensions, which possessed a cilium of $1.2{\;}{\times}{\;}0.19{\;}\mu\textrm{m}$ in size. The pharynx was composed of the epithelial layer of about $0.5{\;}\mu\textrm{m}$ wide, well developed muscle layer and basement layer. The tegument of the oral sucker was composed of a cytoplasmic layer of $0.4-0.5{\;}\mu\textrm{m}$ width, a narrow basement layer, a well developed muscle layer and tegumental cells. Some kinds of secretory granules that seemed to be originated from the cells of the oral sucker were observed In the parenchymal portions of the adjacent cells. The tribocytic organ consisted of numerous microvilli. The microvilli were 5 nm wide and heptalaminated. Two types of secretory granules originated from the gland cells of tribocytic organ were observed In the tegument and parenchyme. The tegumental cells were irregular in shape, and of which nuclei were multifarious.

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Ultrastructural and Immunohistochemical Study of Hepatic Fibrosis after the Ligation of the Common Bile Duct in Rats (백서의 총담관 결찰에 의한 간 섬유화의 초미세구조적 및 면역조직화학적 연구)

  • Moon, Kyung-Rye;Rho, Young-Ill;Seo, Woo-Chul;Park, Yeong-Bong;Kim, Man-Woo;Seo, Jae-Hong;Park, Sang-Kee
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.2 no.2
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    • pp.185-193
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    • 1999
  • Purpose: Proliferation of bile duct-like structures and fibrosis is a hepatic cellular reaction observed in most forms of human liver disease and in a variety of experimental conditions associated with liver injury. The aim of this study was to investigate the activation of Ito cells and bile duct proliferation in the rat after common bile duct ligation (CBDL). Methods: Hepatic morphological abnormalities were examined in rats whose bile ducts had been irreversibly ligated for 15, 21, 24 and 28 days. The liver was examined by immunohistochemical staining for ${\alpha}$-smooth muscle actin, the known marker of activated Ito cells, and light and electron microscopes. Results: After CBDL, the bile canalicular proliferation and interstitial fibrosis were gradually increased in the periportal areas extended to hepatic sinusoids. Ito cells positive for ${\alpha}$-smooth muscle actin were frequently observed in the periductular space and in perisinusoidal space of Disse. Ito cells and myofibroblasts were gradually increased in the interstitial fibrosis until the 28th day after CBDL. Ito cells and myofibroblasts had microfilaments with dense body at the periphery of the cell. Conclusions: Our results suggest that Ito cells may be fibroblastic or myogenic. It has also been postulated that during the development of hepatic fibrosis, Ito cells become myofibroblasts or fibroblast like cells.

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Effect of Addition of Cosolvent γ-Butyrolactone on Morphology of Polysulfone Hollow Fiber Membranes (폴리설폰 중공사막 구조에 대한 조용매 γ-Butyrolactone 첨가 영향)

  • Yun, Sukbok;Lee, Yongtaek
    • Applied Chemistry for Engineering
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    • v.25 no.3
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    • pp.274-280
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    • 2014
  • Polysulfone (PSf) hollow fiber membranes were prepared via the nonsolvent induced phase separation technique. The cosolvent of ${\gamma}$-butyrolactone (GBL) was added to the polymer solution containing a mixture of PSf and N,N-dimethylacetamide (DMAc). Water was utilized as a precipitation nonsolvent. The morphology of prepared membranes was investigated using a field emission scanning electron microscopy. The fabricated membrane showed a typical asymmetric structure such as the dense layer on the porous support layer by the addition of GBL to the polymer solution. As the concentration of GBL increased, the asymmetric porous structure was shown to be more intensified. It was thought that the added GBL played a role of enhancing the liquid-liquid phase separation of the polymer solution, since the cosolvent of GBL might change the thermodynamic solubility parameter of the doping solution. Permeation properties through the prepared hollow fiber membranes were characterized by measuring the pure water flux and the solute rejection using $0.05{\mu}m$ polystyrene latex (PSL) beads. Experimental results revealed that the use of PEG as the internal coagulant enhanced the pure water flux up to 130 times compared to the use of EG while the rejection of the PSL beads decreased only 5%.

Comparison of Grazing Characteristics of a Freshwater Bivalve Unio douglasiae (Unionidae) on the Cold and Warm Phytoplankton Communities in Eutrophic Lake (부영양호수의 저온기와 고온기 식물플랑크톤에 대한 말조개의 섭식능 비교)

  • Lee, Song-Hee;Baik, Soon-Ki;Hwang, Soon-Jin;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.115-123
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    • 2009
  • Grazing effects of a freshwater bivalve, Unio douglasiae, on both the phytoplankton communities of cold- and warm eutrophic lakes were examined in aquarium with sediment. The animal U. douglasiae used in the study was collected from the Gunsan (Jeonbuk), acclimatized in laboratory aquaria with washed sand from the mussel collection stream, and starved for $2{\sim}3$ days by the experiment. Grazing experiments were performed with the surface waters collected from Lake Ilgam (Seoul) in the cold period (March 31, 2008), and the warm period (June 19, 2008), respectively. Results clearly showed that the concentration of chlorophyll-a (chl-a) and algal abundance were decreased with the increase of mussel-treated density and treated-time. At the same animal density, U. douglasiae effectively decreased the chl-a of cold-lake water (CW), compared to warm-lake water (WW). Increasing the mussel density, the filtering rate (or algal abundance) of the mussel on the CW gradually decreased, while those of WW did not increase or decrease. Major phytoplankton species Synedra ulna and Stephanodiscus hantzschii, and Scenedesmus ecornis in CW, and those of Tetraedron regulare and Pediastrum simplex in WW, were quickly decreased after the mussel stocking. Interestingly, cyanobacterium Microcystis aeruginosa in WW, not dominant species, were less removed. These results suggest that a freshwater bivalve U. douglasiae have a strategic potential to control dense hibernal diatom or green algae, rather than the cyanobacterial bloom in eutrophic water.

Vegetation Structure in Otter (Lutra lutra) Home Range of Hwacheon, Gangwon-do (강원도 화천군 수달(Lutra lutra) 서식지의 식생 구조)

  • Seo, Hyungsoo;Shin, Youngseob;Lee, Kyungeun;Kim, Yoonmi;Jeon, Mina;Nam, Taek-Woo;Han, Sung-Yong;Choung, Yeonsook
    • Korean Journal of Ecology and Environment
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    • v.47 no.spc
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    • pp.66-73
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    • 2014
  • In order to determine whether vegetation would be one of the factors for the selection of otter home range, vegetation structure and other potential factors were studied in Hwacheon, Korea. Thirteen sites, otter's activity found and not found, were investigated in North Han River and connected tributary streams of Hwacheon-gun. Three types of vegetation were classified by cluster analysis, which is short grass, tall grass and shrub type. Vegetation zone of each channel is composed of either one type, or mosaic of tall grass and shrub type. Short grass type is common in Lake Paro and upper North Han-river where water level is highly variable throughout a year. Therefore, annual species such as Persicaria nodosa, Fimbristylis dichotomam and Chenopodium ficifolium are the most dominant. Shrub type is common at the downstream sites of Jichon stream and along mainstream of North Han River down Lake Paro. A shrub species, Salix koreensis, is the most common. Tall grass type is dominant occupying the most vegetation zone of the tributary channels. Phragmites japonica is absolutely dominant. Due to its dense cover, a few plant species are co-existed. Otter activity was found in all three vegetation types and no marked activity was found at some sites of tall grass type. There is no difference in species composition and physiognomy between tall grass sites with and without otter activity, while it shows significant difference in fish availability between two groups. Overall we found that home range of otters in the region is along the mainstream and downstream of tributary streams with high fish availability in all vegetation types and in various human activity levels.

The Morphology of the Hemocytes of the Clam, Ruditapes philippinarum (Mollusca: Bivalvia) (한국산 바지락(Ruditapes philippinarum) 혈구의 분류)

  • Kang, Sun-Gu;Park, Sung-Woo;Kim, Young-Gill
    • Journal of fish pathology
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    • v.13 no.2
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    • pp.129-136
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    • 2000
  • To understand host-defence mechanism of clam(Ruditapes philippinarum) hemocyte against foreign materials, classification and their seasonal change in the number were performed. clams collected from a farm in Julpo Bay, Gochang, Chollabuk-Do were used in this experiment. Lots of hemocytes were found between the muscle fibers and connetive tissue of posterior adductor muscle. Hemocytes of R. philippinarum were classified into granulocytes and agranulocytes. Granulocytes were composed of three types, basophilic granulocyte, acidophilic granulocyte and fibrocyte in accordance with the staining affinities of their cytoplasmic granules. Fibrocyte has filopodia and vesicle in endoplasm and bigger than other granulocytes in size. Agranulocytes were less in the number and smaller in the size compared to those of granulocytes. Hyalinocytes had no granule in their cytoplasm. The nucleus located in the center of the cell was oval or spherical shaped. In electron microscopic observation, granulocytes and hyalinocytes contained electron-dense vesicles and some small lucid vesicles in their cytoplasm, respectively. Granulocytes phagocytosed more zymosan particles than hyalinocytes. Acidophilic granulocytes showed higher phagocytic ratio than basophilic granulocytes. Total hemocyte numbers showed the highest at April to August and the lowest at October to December. In the composition of each hemocyte, basophilic granulocytes were always more than acidophilic granulocytes and hyalinocytes.

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Synthesis of ITiO(Indium Titanium Oxide) particle by sol-gel and investigation on light transmittance of deposited ITiO thin film (졸-겔법에 의한 ITiO(Indium Titanium Oxide) 입자의 합성과 ITiO 박막의 광투과도 조사)

  • Go, Eun Ju;Kim, Sang Hern
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.4
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    • pp.705-716
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    • 2017
  • In this study, Indium-Titanium hydroxide particle with 0.5, 1.0, 1.5 wt% of $TiO_2$ were synthesized by sol process and adding the base, ITiO(Indium Titanium Oxide) particles were obtained by gelling at $200^{\circ}C$ and $500^{\circ}C$. The ITiO particle's size with gel process at $200^{\circ}C$ was smaller than ITiO particle's size with gel process $500^{\circ}C$. The ITiO particle with gel process at $200^{\circ}C$ was used to fabricate dense ITiO target. ITiO targets with 0.5, 1.0, 1.5 wt% of $TiO_2$ were fabricated and used to obtain ITiO thin films onto glass by sputtering. Among those sputtered ITiOs' thin films, ITiO thin film with 0.4 % of $O_2$ and 0.5 wt% of $TiO_2$ showed the lowest specific resistance, highest charge mobility and lowest carrier concentration. It was found the light transmittance of the ITiO film were increased highly compared to light transmittance of ITO (Indium Tin Oxide) thin film over Infrared wavelength ranges.

The Properties of OPC-Slag Cement Mixed with Nano-Silica Solution by Mixing Water Weight Replacement Method (나노실리카 졸을 배합수 중량치환 방법으로 혼합한 OPC-slag cement의 특성)

  • Seo, Ki-Young;Kim, Taewan;Kim, Seong-Do
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.1
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    • pp.24-34
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    • 2020
  • This research is a study on the characteristics of OPC-slag cement using nano-silica solution (NSS) with water-weight substitution method. The new replacement method is a fundamental step to study the behavior of cement with higher NSS replacement rates than previous studies. NSS was replaced by 10%, 20%, 30%, 40%, and 50% of the mixing water weight. As a result, the mechanical and microstructural characteristics were improved. This can be summarized in two ways. First, when the NSS is replaced with mixing water, the homogeneous dispersion action of the nano-silica particles is improved. This promotes initial hydration. Second, substitution of NSS with higher density than mixing water reduces w / b. This forms a dense hydration reaction material. The new substitution method did not show any degradation of mechanical and microstructural properties as compared with the results using the powdered nano-silica particles revealed in the previous study. Therefore, it is considered that the method of weight substitution of NSS used in this study can be applied to the formulation of OPC-slag cement.

An Experimental Study on the Creep Behavior of Frozen Sand (동결 사질토의 크리프 거동에 관한 실험적 연구)

  • Chae, Deokho;Kim, Youngseok;Lee, Jangguen;Cho, Wanjei
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.2
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    • pp.27-36
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    • 2014
  • Due to the latitudinal location of Korea, the seasonally frozen ground has been focused on as research topics such as the frost heaving under the asphalt road rather than the permafrost ground. However, the recent construction of the second Korean Antarctic research station, the Jangbogo station and the participation on the development of the natural gas pipeline in Russia arouse the research interests on the behavior of the permafrost ground. At the design process of the geotechnical structures on the permafrost ground, the evaluation of the creep characteristics of the frozen soil is very crucial. Since the domestic specification on the frozen soil testing does not exist currently, it is necessary to evaluate the creep characteristics of frozen soils systematically with regard to the affecting factors. Therefore, the creep characteristics of the frozen specimens of dense Jumoonjin sand were evaluated under various loads at -5 and $-10^{\circ}C$. Based on the test results, as the load became close to the strength and the temperature became lower, the duration of the secondary creep became shorter and more distinct tertiary creep responses were observed.

Low-Temperature Sintering and Microwave Dielectric Properties of the $B_2O_3-$ and CuO-added $Ba(Mg_{1/3}Nb_{2/3})O_3$ Ceramics ($B_2O_3$ 와 CuO가 첨가된 $Ba(Mg_{1/3}Nb_{2/3})O_3$ 세라믹스의 저온소결과 마이크로파 유전특성 연구)

  • Lim, Jong-Bong;Son, Jin-Ok;Nahm, Sahn;Yu, Myeong-Jae;Lee, Woo-Sung;Kang, Nam-Kee;Lee, Hwack-Joo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2004.07b
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    • pp.838-841
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    • 2004
  • [ $B_2O_3$ ] added $Ba(Mg_{1/3}Nb_{2/3})O_3$ (BBMN) ceramics were not sintered below $900^{\circ}C$. However, when CuO was added to the BBMN ceramic, it was sintered even at $850^{\circ}C$. The amount of the $Ba_2B_2O_5$ second phase decreased with the addition of CuO. Therefore, the CuO additive is considered to react with the $B_2O_3$ inhibiting the reaction between $B_2O_3$ and BaO. Moreover, it is suggested that the solid solution of CuO and $B_2O_3$ might be responsible for the decrease of the sintering temperature of the specimens. A dense microstructure without pores was developed with the addition of a small amount of CuO. However, a porous microstructure with large pores was formed when a large amount of CuO was added. The bulk density the dielectric constant $({\varepsilon}_r)$ and the Q-value increased with the addition of CuO but they decreased when a large amount of CuO was added. The variations of those properties are closely related to the variation of the microstructure. The excellent microwave dielectric properties of Qxf=21500 GHz, ${\varepsilon}_r=31$ and temperature coefficient of resonance frequency$({\tau}_f)=21.3\;ppm/^{\circ}C$ were obtained for the $Ba(Mg_{1/3}Nb_{2/3})O_3+2.0\;mol%B_2O_3+10.0$ mol%CuO ceramic sintered at $875^{\circ}C$ for 2h.

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