• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.94-94
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• 2017

Oryza sativa and Oryza glaberrima are rice cultivars distributed in Asia and Africa. There are several differences between these cultivars in morphological characteristics such as panicle structure and especially the secondary branch number of O. glaberrima is less than that of O. sativa. Generally, branch number of panicle related to a large vascular bundle number (VBN) among O. sativa and there is a wide variation of the VBN of the peduncle from where the bundles enter into the rachis branches. However, there is less information about VBN in O. glaberrima and also the relationship between VBN and branch numbers, the primary branch number (PBN) and secondary branch number (SBN). Additionally, the genetic factor for VBN and branch number in O. glaberrima is not completely exploited. In this study, phenotypic variation for VBN and panicle structure were investigated using a set of 40 $BC_3$ -derived from IRGC 104038 (O. glaberrima from Senegal) and 35 $BC_4$ -derived from IRGC103777 (O. glaberrima from Mali) introgression lines with a genetic background of japonica rice Taichung 65. Taichung 65 had 11.8 PBN, 16.0 SBN and 11.5 VBN, while IRGC 103777 had 12.0 PBN, 15.0 SBN and 15.3 VBN. The introgression lines derived from IRGC 104038 had range from 9.0 to 14.4 in the PBN, range from 9.6 to 33.5 in the SBN and range from 9.8 to 14.8 in the VBN. Additionally, the introgression lines derived from IRGC 103777 had range from 9.0 to 18.5 in the PBN, range from 10.3 to 39.0 in the SBN and range from 9.0 to 15.3 in the VBN. Among two set of introgression lines, there are significant correlation between VBN and PBN. Multiple introgression lines indicated higher PBN, SBN and VBN than Taichung 65 and these examined characteristics are supposedly controlled by quantitative traits loci. The genetic factor related to VBN and panicle architecture can be revealed using segregating population in future study.

• Molecules and Cells
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• v.39 no.10
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• pp.768-775
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• 2016

The Arabidopsis female gametophyte contains seven cells with eight haploid nuclei buried within layers of sporophytic tissue. Following double fertilization, the egg and central cells of the gametophyte develop into the embryo and endosperm of the seed, respectively. The epigenetic status of the central cell has long presented an enigma due both to its inaccessibility, and the fascinating epigenome of the endosperm, thought to have been inherited from the central cell following activity of the DEMETER demethylase enzyme, prior to fertilization. Here, we present for the first time, a method to isolate pure populations of Arabidopsis central cell nuclei. Utilizing a protocol designed to isolate leaf mesophyll protoplasts, we systematically optimized each step in order to efficiently separate central cells from the female gametophyte. We use initial manual pistil dissection followed by the derivation of central cell protoplasts, during which process the central cell emerges from the micropylar pole of the embryo sac. Then, we use a modified version of the Isolation of Nuclei TAgged in specific Cell Types (INTACT) protocol to purify central cell nuclei, resulting in a purity of 75-90% and a yield sufficient to undertake downstream molecular analyses. We find that the process is highly dependent on the health of the original plant tissue used, and the efficiency of protoplasting solution infiltration into the gametophyte. By isolating pure central cell populations, we have enabled elucidation of the physiology of this rare cell type, which in the future will provide novel insights into Arabidopsis reproduction.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.76-81
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• 1994

This experiment was carried out to select the best adaptable varieties of Tall fescue in mountainous pastures of Taekwalyon area. The evaluation was based on the data of varietial differences in the dry weight of plant@W) and yield components of the 2nd growth. Nine varieties of Tall fescue examined were Barcel, Barvetia, Demeter, Enforcer, Forager, Fuego, Johnstone, Safe and Stef. And, the final cutting times were Sept. 30 ($C_1$), Oct.14 ($C_2$),Oct. 28 ($C_3$), and Nov. 11($C_4$). The reults were summarized as follows: 1. Barcel and Forager with many tillers per plant(NT) showed a high dry weight of plant@W) of the 2nd growth. 2. The dry weight of plant(DW) of the 2nd growth indicated significantly positive correlation with the number of tillers per plant(NT), but there was not significant correlation with the dry weight of tiller(WT). 3. The maximum dry weight of plant@W) of the 2nd growth was obtained at $C_3$(28 Oct.). 4. The dry weight of the 2nd growth showed an inverse relations with the dry weight of the 1st growth as affected by various final cutting time in growth period of autumn.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.203-212
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• 1993

This experiments were carried out to estimate the optimum final cutting date in autumn and the selection of highly adaptable varieties in mountainous pastures of Taekwalyong area. The evaluations were based on the data of varietal differences of dry weight of plant(DW) and yield components of the 1st cutting as affected by various final cutting dates of last autumn. Nine varieties of tall fescue Barvetia, Fuego. Demeter, Safe. Barcel, Forager, Johnstone, Enforcer and Stef. were used and 4 final cutting date of autumn were $C_1$, cut on 30 Sept. $C_2$, on 14 Oct. $C_3$, on 28 Oct. and $C_4$. on 11 Nov.. respectively. The results obtained were as follows: I . Between the dry weight of plant(DM) and yield components of 1st cutting were different by various final cutting dates of autumn. The dry weight of plant(DW) was significantly positive correlated with heading characteristics of the 1st cutting in earlier cuttings($C_1- C_3$) of autumn, but there was not significant correlated with heading characteristics of the 1st cutting in the latest cutting($C_4$) of autumn. 2. The variety of Forager with heavier weight of heading tiller(HTW) and dry weight of tiller(WT) showed the highest dry weight of plant(DW) of the 1st cutting in earlier cuttings($C_1-C_3$) of autumn, whereas the variety of Barcel with high number of tillers per plant(NT) showed a high dry weight of plant(DW) of the 1st cutting in the latest cutting($C_4$) of autumn. 3. Optimum final cutting date and critical period of mountainous pastures in Taekwalyong area were estimated in late September($C_1$) and middle October($C_2$), sespectirety. 4. The dry weight of plant(DW) of the 1st cutting was significantly negative correlated with final cutting dates in growth period of autumn.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.213-220
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• 1993

This experiments were carries out to estimate the optimum final cutting date in autumn and the selection of highly adaptable varieties in mountainous pastures of Taekwalyong area. The evaluations were based on the data of varietal differences of dry weight of lant(DW) and yield components of the 1st cutting as affected by various final cutting dates of last autumn. Nine varieties of fall fescue Barvetia, Fuego, Demeter, Safe Barcel Forager, Johnstone, Enforcer and Stef, were used and 4 final cutting date of autumn were $C_1$, cut on 30 Sept. $C_2$, on 14 Oct. $C_3$, on 28 Oct. and $C_4$ on 11 Nov.. respectively. The results obtained were as follows; 1. Between the dry weight of plant(DM) and yield components of 1st cutting were different by various final cutting dates of autumn. The dry weight of plant(DW) was significantly positive correlated with heading characteristics of the 1st cutting in earlier cuttings$(C_1-C_3)$ of autumn, but there was not significant correlated with heading characteristics of the 1st cutting in the latest cutting$(C_4)$ of autumn. 2. The variety of Forager with heavier weight of heading tiller(HTW) and dry weight of tiller(WT) showed the highest dry weight plant(DW) of the 1st cutting in earlier cuttings$(C_1-C_3)$ of autumn, whereas the variety of Barcel with high number of tillers per plant(NT) showed a high dry weight of plant(DW) of the 1st cutting in the latest cutting$(C_4)$ of autumn. 3. Optimum final cutting date and critical period of mountainous pastures in Taekwalyong area were estimated in late September$(C_1)$ and middle October$(C_2)$, sespectirety. 4. The dry weight of plant(DW) of the 1st cutting was significantly negative correlated with final cutting dates in growth period of autumn.